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        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
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        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="OAV94486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblFungi" id="PTTG_03045-t43_2">
        <property type="protein sequence ID" value="PTTG_03045-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03045"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03045"/>
    <dbReference type="OMA" id="SIECIDM"/>
    <dbReference type="OrthoDB" id="273556at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:protein ubiquitination"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:Ras protein signal transduction"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
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    <dbReference type="InterPro" id="IPR011422">
        <property type="entry name" value="BRAP2/ETP1_RRM"/>
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        <property type="entry name" value="RBD_domain_sf"/>
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    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="Znf_UBP"/>
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    <dbReference type="PANTHER" id="PTHR24007">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR24007:SF7">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
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        <location>
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            <end position="537"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="5">
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50089"/>
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            <dbReference type="EnsemblFungi" id="PTTG_03045-t43_2-p1"/>
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            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
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            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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            <fullName evidence="3">LysM domain-containing protein</fullName>
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        <name evidence="2" type="ORF">PTTG_03085</name>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000039">
        <property type="protein sequence ID" value="OAV94398.1"/>
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    </dbReference>
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    <dbReference type="EnsemblFungi" id="PTTG_03085-t43_2">
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        <property type="gene ID" value="PTTG_03085"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03085"/>
    <dbReference type="OrthoDB" id="2107166at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
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        <location>
            <begin position="1"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="83"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV94398.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03085-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="126" mass="13637" checksum="65F98C9A191D2C82" modified="2015-04-01" version="1">MPASLTHHPLTTTTTPAAEQPQASTSHASPDITRPTLKRLTADQQPHTPDTASQIWKTKQPPSRTNGKQPSTQPTTPLANNPNRIKGDRDVLIHRVERGHSLPGIALSYGISVQSPLLPIARPPSR</sequence>
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    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_04212</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000037">
        <property type="protein sequence ID" value="OAV94696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4ETT3"/>
    <dbReference type="EnsemblFungi" id="PTTG_04212-t43_2">
        <property type="protein sequence ID" value="PTTG_04212-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04212"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04212"/>
    <dbReference type="OrthoDB" id="1670005at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004721">
        <property type="entry name" value="DHOdimr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137:SF1">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OAV94696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04212-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="167" mass="18234" checksum="8A83FA7ACE74A9A1" modified="2015-04-01" version="1">MTSIDSIVIPCPFDAHVHLRQPGELLELVVAQIASGGIQSVYVMPNTVPPITTLEMAVKYRQTLEKLEPSIQFFISLYLSPSLTPSDIRRAKACGVSGVKSYPRGVTTNSAEGVESYEIYYPVFEAMEQENLVLNLHGEVPSTQTGVSVFSTTRTSLSQDFYGGLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4EU09</accession>
    <name>A0A0C4EU09_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_04288</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000043">
        <property type="protein sequence ID" value="OAV94115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4EU09"/>
    <dbReference type="EnsemblFungi" id="PTTG_04288-t43_1">
        <property type="protein sequence ID" value="PTTG_04288-t43_1-p1"/>
        <property type="gene ID" value="PTTG_04288"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04288"/>
    <dbReference type="OMA" id="NDHCRRR"/>
    <dbReference type="OrthoDB" id="2431475at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40132">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40132:SF1">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV94115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04288-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="222" mass="25798" checksum="ED5227F140D9FD70" modified="2015-04-01" version="1">MDKYFAAGYDPRLDVNLDDITNPQTGIIEGGNYDDWEIILAQMKAKRDERAREKERKLELKLHAAQRKLRKSEKKEHHRSLKSRSKGKRSKDASDDELDDLDRERRRRKRNEKKKKKERRDHSASKTDQDNIDDNDREKRRRKKGKQRNSKPGSESDSSSSSSSDSHSSSTSSSSNDHCRRRHREPSGKDRYDTKSAGLIAFNGFSKLGTLREWDRGKPSPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4EU19</accession>
    <name>A0A0C4EU19_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SH3 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_04298</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
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                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
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                <person name="Sakthikumar S."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
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                <person name="Feldgarden M."/>
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                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
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                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
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                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
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                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Khalil H.B."/>
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                <person name="Sakthikumar S."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
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                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
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                <person name="Szabo L.J."/>
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                <person name="Feldgarden M."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
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                <person name="Khalil H.B."/>
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                <person name="Linning R."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
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                <person name="Szabo L.J."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000015">
        <property type="protein sequence ID" value="OAV97094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4EY84"/>
    <dbReference type="EnsemblFungi" id="PTTG_05783-t43_1">
        <property type="protein sequence ID" value="PTTG_05783-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05783"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05783"/>
    <dbReference type="OMA" id="IQFIGHY"/>
    <dbReference type="OrthoDB" id="2124888at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046521">
        <property type="term" value="P:sphingoid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009305">
        <property type="entry name" value="Mpo1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28026:SF9">
        <property type="entry name" value="2-HYDROXY-PALMITIC ACID DIOXYGENASE MPO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28026">
        <property type="entry name" value="DUF962 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G05310)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06127">
        <property type="entry name" value="Mpo1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV97094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05783-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="26816" checksum="1C4F7D9D7F8CA1A9" modified="2015-04-01" version="1">MSSTREQQSKTAIDQDTPLLPTTKSYSIFNLEDQFLFYGQYHHNPVNILIHLICVPVIFFTSLILAHQFGFFPHTVLGAVELPELLVRTGWFGQTGAMYELNMSTVTSIGYALYFVALEPVAGLLYMPILLAFGHWANLIVTSFPDSYFNPTLAIWVLSWILQFVGHGHFEKRKPALIDNLFQSIVLAVFFVWIEALFFLGYKPALARSIHRKIAAAVAQFKASSPSHSPQSPPPSKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4EYW2</accession>
    <name>A0A0C4EYW2_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AMP-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_06017</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000097">
        <property type="protein sequence ID" value="OAV90647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4EYW2"/>
    <dbReference type="EnsemblFungi" id="PTTG_06017-t43_1">
        <property type="protein sequence ID" value="PTTG_06017-t43_1-p1"/>
        <property type="gene ID" value="PTTG_06017"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06017"/>
    <dbReference type="OMA" id="RWEPVFH"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF83">
        <property type="entry name" value="ACYL-COA SYNTHETASE LONG-CHAIN, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="6">
        <location>
            <begin position="95"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV90647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06017-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="679" mass="74524" checksum="FB827001085CB041" modified="2015-04-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4F4R4</accession>
    <name>A0A0C4F4R4_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_08104</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000016">
        <property type="protein sequence ID" value="OAV96920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4F4R4"/>
    <dbReference type="EnsemblFungi" id="PTTG_08104-t43_2">
        <property type="protein sequence ID" value="PTTG_08104-t43_2-p1"/>
        <property type="gene ID" value="PTTG_08104"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08104"/>
    <dbReference type="OrthoDB" id="2495796at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    <proteinExistence type="predicted"/>
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        <source>
            <dbReference type="EMBL" id="OAV96920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08104-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="458" mass="51084" checksum="3F3A7E78609C7A62" modified="2015-04-01" version="1">MDVLKEELELTDEADGDQVNTLIRRAHFVRFPERDASTLTEDQMLAALDGSTASAGEKLKPADQIQWATDIVRQGFDAEYQRHDLIVAPTLEKLQRFAGQWKRDKYEAPYTLLIGPTMSGKTRLLKELAKHVCVAYICLRPLGSTGQPPRSELATHFFPNGKARLDLEKHYSYLLAAILNTISEFFSRPDIRARSLEDRLAAWFDYSFQMDKTPKAEYNANVEKEMKSIQAEGLAPMQFLGAAATRMSQSIAFEGDRRLKMLLAIDEAGNLDNQIDKELQIPYFCVFRRALSHIPAKLGFFGVVTDTTLRVANFNPVLTQDSSSRCLALGVDLFPPIYQISSPDVFVPKALSSWDELLSAERLFSYGSPFYGLYFKGVMRGVMKGVPGTAVESTSKIAEAKLVCSSQLVSTEAMELSSPQCFAILGSVIQTRVCLYSPINSELVANHTRYAENQGNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4F530</accession>
    <name>A0A0C4F530_PUCT1</name>
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        <submittedName>
            <fullName evidence="11">CAMK/CAMK1/CAMK1-RCK protein kinase, variant</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PTTG_08224</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000412">
        <property type="protein sequence ID" value="OAV87431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4F530"/>
    <dbReference type="EnsemblFungi" id="PTTG_08224-t43_1">
        <property type="protein sequence ID" value="PTTG_08224-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08224"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08224"/>
    <dbReference type="OMA" id="KQYYYIV"/>
    <dbReference type="OrthoDB" id="1738954at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14096">
        <property type="entry name" value="STKc_RCK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="167"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="541"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="592"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="542"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="594"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="606"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="294"/>
            <end position="295"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OAV87431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08224-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="663" mass="73940" checksum="F85EBE02CA0DD3DF" modified="2015-04-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4F5T4</accession>
    <name>A0A0C4F5T4_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ceramide glucosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.80</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Glucosylceramide synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">UDP-glucose ceramide glucosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">UDP-glucose:N-acylsphingosine D-glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PTTG_08487</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="17">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.80" evidence="5"/>
    <dbReference type="EMBL" id="ADAS02000027">
        <property type="protein sequence ID" value="OAV95572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4F5T4"/>
    <dbReference type="EnsemblFungi" id="PTTG_08487-t43_2">
        <property type="protein sequence ID" value="PTTG_08487-t43_2-p1"/>
        <property type="gene ID" value="PTTG_08487"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08487"/>
    <dbReference type="OMA" id="HELRWNR"/>
    <dbReference type="OrthoDB" id="1483400at2759"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008120">
        <property type="term" value="F:ceramide glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006679">
        <property type="term" value="P:glucosylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02520">
        <property type="entry name" value="Glucosylceramide_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000159">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.48, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025993">
        <property type="entry name" value="Ceramide_glucosylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726:SF0">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13506">
        <property type="entry name" value="Glyco_transf_21"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="254"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="422"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="437"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="474"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OAV95572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08487-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="547" mass="60645" checksum="129FBE9BF9F8B685" modified="2015-04-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4F856</accession>
    <name>A0A0C4F856_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="8">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PTTG_09343</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="8"/>
    <dbReference type="EMBL" id="ADAS02000051">
        <property type="protein sequence ID" value="OAV93368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4F856"/>
    <dbReference type="EnsemblFungi" id="PTTG_09343-t43_2">
        <property type="protein sequence ID" value="PTTG_09343-t43_2-p1"/>
        <property type="gene ID" value="PTTG_09343"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09343"/>
    <dbReference type="OMA" id="TWMCEAH"/>
    <dbReference type="OrthoDB" id="17199at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000003">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039799">
        <property type="entry name" value="ALR/ERV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645">
        <property type="entry name" value="ALR/ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645:SF0">
        <property type="entry name" value="FAD-LINKED SULFHYDRYL OXIDASE ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="10">
        <location>
            <begin position="89"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OAV93368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09343-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="200" mass="22541" checksum="E5BB40707768158B" modified="2015-04-01" version="1">MDPSTSSTFVASSSSETSPPLPNKNAKKRNPAGVLGPDGKPCKVCSGFQAWTKQAKQDVKQDEMKGHSKEARIEEGTTERTIVEERADCPADSNRLGRHTWTLLHTIGSYYPVERPSTAQKDSVRQLINSLATIYPCQPCASHLQDYLHRFPPKTDNRSQLERWLCDAHNEVNERLGKELFDCSLVSKRWRDGWDDGHCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180FZ31</accession>
    <name>A0A180FZ31_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Zf-4CXXC_R1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PTTG_30375</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02003219">
        <property type="protein sequence ID" value="OAV85640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180FZ31"/>
    <dbReference type="EnsemblFungi" id="PTTG_30375-t43_1">
        <property type="protein sequence ID" value="PTTG_30375-t43_1-p1"/>
        <property type="gene ID" value="PTTG_30375"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_30375"/>
    <dbReference type="OrthoDB" id="2497737at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040221">
        <property type="entry name" value="CDCA7/CDA7L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018866">
        <property type="entry name" value="Znf-4CXXC_R1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31169">
        <property type="entry name" value="OS05G0300700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31169:SF8">
        <property type="entry name" value="ZINC-FINGER DOMAIN OF MONOAMINE-OXIDASE A REPRESSOR R1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10497">
        <property type="entry name" value="zf-4CXXC_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Zinc-finger" evidence="11">
        <location>
            <begin position="57"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="137"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="264"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
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            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
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            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
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        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="321"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF10497"/>
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        <source>
            <dbReference type="EMBL" id="OAV85640.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblFungi" id="PTTG_30375-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180FZ40</accession>
    <name>A0A180FZ40_PUCT1</name>
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        <recommendedName>
            <fullName evidence="3">COX assembly mitochondrial protein</fullName>
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    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_30343</name>
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    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for mitochondrial cytochrome c oxidase (COX) assembly and respiration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the CMC family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02003011">
        <property type="protein sequence ID" value="OAV85685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180FZ40"/>
    <dbReference type="EnsemblFungi" id="PTTG_30343-t43_1">
        <property type="protein sequence ID" value="PTTG_30343-t43_1-p1"/>
        <property type="gene ID" value="PTTG_30343"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_30343"/>
    <dbReference type="OrthoDB" id="532630at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Cyt_c_biogenesis_Cmc1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08583">
        <property type="entry name" value="Cmc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV85685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_30343-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="59" mass="6852" checksum="92F5574749203ED5" modified="2016-09-07" version="1">MHPHLTGNKVEACGPIIEALHQCHQSGFWTYYTGGCNDIRRQLDKCLHAEVEWQDLRLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180FZ66</accession>
    <name>A0A180FZ66_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">chitin deacetylase</fullName>
            <ecNumber evidence="9">3.5.1.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PTTG_30323</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="14">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + n H2O = chitosan + n acetate</text>
            <dbReference type="Rhea" id="RHEA:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9597"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57704"/>
            <dbReference type="EC" id="3.5.1.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:10465"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
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    <dbReference type="EC" id="3.5.1.41" evidence="9"/>
    <dbReference type="EMBL" id="ADAS02002904">
        <property type="protein sequence ID" value="OAV85714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180FZ66"/>
    <dbReference type="EnsemblFungi" id="PTTG_30323-t43_1">
        <property type="protein sequence ID" value="PTTG_30323-t43_1-p1"/>
        <property type="gene ID" value="PTTG_30323"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_30323"/>
    <dbReference type="OrthoDB" id="407355at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004099">
        <property type="term" value="F:chitin deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
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    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050248">
        <property type="entry name" value="Polysacc_deacetylase_ArnD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10587:SF135">
        <property type="entry name" value="CHITIN DEACETYLASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10587">
        <property type="entry name" value="GLYCOSYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="6" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008109461" description="chitin deacetylase" evidence="11">
        <location>
            <begin position="33"/>
            <end position="378"/>
        </location>
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    <feature type="domain" description="NodB homology" evidence="12">
        <location>
            <begin position="162"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048494"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OAV85714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_30323-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180FZY2</accession>
    <name>A0A180FZY2_PUCT1</name>
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        <submittedName>
            <fullName evidence="14">PHYTOCHROME_2 domain-containing protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="13" type="ORF">PTTG_30150</name>
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    <organism evidence="13">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="14">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02002006">
        <property type="protein sequence ID" value="OAV85985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180FZY2"/>
    <dbReference type="EnsemblFungi" id="PTTG_30150-t43_2">
        <property type="protein sequence ID" value="PTTG_30150-t43_2-p1"/>
        <property type="gene ID" value="PTTG_30150"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_30150"/>
    <dbReference type="OrthoDB" id="2015534at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009584">
        <property type="term" value="P:detection of visible light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
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    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
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    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
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    <dbReference type="InterPro" id="IPR016132">
        <property type="entry name" value="Phyto_chromo_attachment"/>
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    <dbReference type="InterPro" id="IPR001294">
        <property type="entry name" value="Phytochrome"/>
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    <dbReference type="InterPro" id="IPR013515">
        <property type="entry name" value="Phytochrome_cen-reg"/>
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    <dbReference type="InterPro" id="IPR043150">
        <property type="entry name" value="Phytochrome_PHY_sf"/>
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    <dbReference type="PANTHER" id="PTHR43065:SF10">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065">
        <property type="entry name" value="SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
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    <dbReference type="Pfam" id="PF00360">
        <property type="entry name" value="PHY"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PHYTOCHROME_2"/>
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    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0157">Chromophore</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="1" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Phytochrome chromophore attachment site" evidence="12">
        <location>
            <begin position="15"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="386"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="442"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="442"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OAV85985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_30150-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="465" mass="51861" checksum="5E073910899C414A" modified="2016-09-07" version="1">MLYNRDQPTARLMCKSQEELSKPLNMTHCHLRAMSPIHIKYLANMGVRSSFLILIIMNDELWGLISCHSYGRFGQRVTFPTRQFCTMLGDTVSRNILRLNLSQRLQSRKLINTTSTSKNPSGYIVTKAEDLLWLFDAQSGVLSIGEEAKILGTVSNSQEILAILEYLRVRKYDSIQVSTEINVTFPDIEYPGGFELISGVLVIPLSRSGQDFICFFRPAQSKEIHWAGNPHEKIMKDDKNGNILEPRKSFKIWSETVKERSKAWTADQLETGSVLSLVYGKFIDVWRQKEAAVQNNQLQALLLSNASHEVRTPLHQILSTLELALDGQLDEDTRDNLLKSYTASRALVHVINDLLHFSRSSVISHTSSTRGPPTIEQPVLIAGMSHAAPSAAAPSSRSDPSQCSSPPTILSDPDSYITTRFEYQQGEDGLLILTGRRDQASNARYANATSDPTTPSAQPHSMALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G2A8</accession>
    <name>A0A180G2A8_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleolar 27S pre-rRNA processing Urb2/Npa2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_12580</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000867">
        <property type="protein sequence ID" value="OAV86718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_12580-t43_1">
        <property type="protein sequence ID" value="PTTG_12580-t43_1-p1"/>
        <property type="gene ID" value="PTTG_12580"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12580"/>
    <dbReference type="OrthoDB" id="2499190at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="534"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV86718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12580-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1290" mass="145096" checksum="A1E826A93619D509" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G3F5</accession>
    <name>A0A180G3F5_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_06142</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000521">
        <property type="protein sequence ID" value="OAV87225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G3F5"/>
    <dbReference type="EnsemblFungi" id="PTTG_06142-t43_1">
        <property type="protein sequence ID" value="PTTG_06142-t43_1-p1"/>
        <property type="gene ID" value="PTTG_06142"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06142"/>
    <dbReference type="OrthoDB" id="106784at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase RBBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033489">
        <property type="entry name" value="RBBP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025829">
        <property type="entry name" value="Zn_knuckle_CX2CX3GHX4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439:SF0">
        <property type="entry name" value="CELL DIVISION CYCLE AND APOPTOSIS REGULATOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13696">
        <property type="entry name" value="zf-CCHC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
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                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
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                <person name="Goldberg J."/>
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                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
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                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
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                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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                <person name="Zeng Q."/>
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                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
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                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Khalil H.B."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
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            <dbReference type="EMBL" id="OAV87595.1"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12406-t43_2-p1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
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    </evidence>
    <sequence length="420" mass="47538" checksum="0EF8643469835BC4" modified="2016-09-07" version="1">MKRTRGLSHDIMPPAGADPFADISHLAAHRFTARLPQSAIILLAVFLVFHILIAAFNLAILIFPYVGRAKRQLWTYKKLYIYDRSGEKLYSTPLYLVNAGVLMSVSQLLSSVTTQVYILMHIRMNLSEHYALRCQFIPVLGLVFIFDTYSYWSMAHCFLALYYSNKNFAVKPKRLSWLRSPRSINIFFLIFPIFVTVATIIVITRMSMVYHDLQVQTTSLRATLAQGTLIWKQLELPGHSAEEIASLSSQLTTVQSRLGSLLVPTGTLALKLLDRFNSIRSVMLFFIPATSLVFVLSFWKLAQKYKSRGDPPTELISKADLSDSWHAQSNSQHISVRSHVSPTSLTLFQTLQSDPQFLRLTIRAFATLLGMLVALVFWVLAIFKSNDMMIDPFWHGLATWLPAVSGTWTAIPLAWQSVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G4J4</accession>
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                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000355">
        <property type="protein sequence ID" value="OAV87561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G4J4"/>
    <dbReference type="EnsemblFungi" id="PTTG_05363-t43_2">
        <property type="protein sequence ID" value="PTTG_05363-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05363"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05363"/>
    <dbReference type="OrthoDB" id="2502977at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025256">
        <property type="entry name" value="TM7S3/TM198-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13886">
        <property type="entry name" value="TM7S3_TM198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008109549" evidence="7">
        <location>
            <begin position="26"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="TM7S3/TM198-like" evidence="8">
        <location>
            <begin position="64"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="321"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="359"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="389"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13886"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OAV87561.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05363-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G5H7</accession>
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            <fullName evidence="14">sn-1-specific diacylglycerol lipase</fullName>
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        <name evidence="17" type="ORF">PTTG_03325</name>
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    <organism evidence="17">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="19">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycerol + H2O = a 2-acylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33275"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17389"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.116"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:33276"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.1.116" evidence="14"/>
    <dbReference type="EMBL" id="ADAS02000268">
        <property type="protein sequence ID" value="OAV87844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G5H7"/>
    <dbReference type="EnsemblFungi" id="PTTG_03325-t43_2">
        <property type="protein sequence ID" value="PTTG_03325-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03325"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03325"/>
    <dbReference type="OrthoDB" id="438440at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046340">
        <property type="term" value="P:diacylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052214">
        <property type="entry name" value="DAG_Lipase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45792">
        <property type="entry name" value="DIACYLGLYCEROL LIPASE HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45792:SF8">
        <property type="entry name" value="DIACYLGLYCEROL LIPASE-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="16">
        <location>
            <begin position="422"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="379"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OAV87844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03325-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="759" mass="84320" checksum="614ABB4A409C25C5" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G5L2</accession>
    <name>A0A180G5L2_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">[histone H3]-trimethyl-L-lysine(4) demethylase</fullName>
            <ecNumber evidence="4">1.14.11.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PTTG_09541</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="19 20" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="20">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 2-oxoglutarate + 3 O2 = L-lysyl(4)-[histone H3] + 3 formaldehyde + 3 succinate + 3 CO2</text>
            <dbReference type="Rhea" id="RHEA:60208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.67" evidence="4"/>
    <dbReference type="EMBL" id="ADAS02000253">
        <property type="protein sequence ID" value="OAV87908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G5L2"/>
    <dbReference type="EnsemblFungi" id="PTTG_09541-t43_1">
        <property type="protein sequence ID" value="PTTG_09541-t43_1-p1"/>
        <property type="gene ID" value="PTTG_09541"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09541"/>
    <dbReference type="OrthoDB" id="1678912at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034647">
        <property type="term" value="F:histone H3K4me/H3K4me2/H3K4me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16100">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15543">
        <property type="entry name" value="PHD_RSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000016">
        <property type="entry name" value="Putative Lysine-specific demethylase 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048615">
        <property type="entry name" value="KDM5_C-hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004198">
        <property type="entry name" value="Znf_C5HC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF33">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21323">
        <property type="entry name" value="KDM5_C-hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02928">
        <property type="entry name" value="zf-C5HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="16">
        <location>
            <begin position="33"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="ARID" evidence="15">
        <location>
            <begin position="98"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="14">
        <location>
            <begin position="305"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="17">
        <location>
            <begin position="474"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="200"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="773"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="811"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1034"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1112"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="219"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="825"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="870"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="937"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="979"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1132"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1145"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="1186"/>
            <end position="1195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OAV87908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09541-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="1195" mass="133250" checksum="6FEA7CCD95095F6D" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G6N0</accession>
    <name>A0A180G6N0_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AMP_N domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_08456</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000192">
        <property type="protein sequence ID" value="OAV88264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G6N0"/>
    <dbReference type="EnsemblFungi" id="PTTG_08456-t43_1">
        <property type="protein sequence ID" value="PTTG_08456-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08456"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08456"/>
    <dbReference type="OrthoDB" id="4215474at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226:SF4">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase P N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV88264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08456-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="481" mass="53086" checksum="A1538CFCF8BBACEF" modified="2016-09-07" version="1">MEMLPDGSAVIIAGARTKWMAHHVFYPFRQSSDFWYLTGFQEPDSCLLLEKGNSTKGYTMTMFVRPKDPFQEMWEGARSGIDGVTEWFGADITYDISDLASRLKPVLTKSTNSPVYLDLPSDIEFPNLPPWKSTVPLIDFFSKNNSQTGNLTDFLSSAPGNFSSSSRSDLDSCLAVLATSRSVNRGQTNQSKYRPIRSLQAKLDSIKMIKSEAELKLLRMAADISAHGFKAAMRLAAECDSEGSRSEHDLVQTFEGACHQHSPGVGGRMGYVPVCAAGPAALTIHYTFNDRALKPGNQLVLLDAGFEYAGYVADITRTFPVGNKGKFTSAQKDLYEVVKNAEKELITQCHQGSGHSLSSLHRASCQLLKEGLVKLGFNLDHPDALNRLYPHYIGHPVGTDLHDTPTWNRSHRLKAGSVITIEPGVYVPDEDRYPKHFRGIGIRVEDMVHVRETDQVILSCNTPKEVVDVEACGSGLLDGIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G7J0</accession>
    <name>A0A180G7J0_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CCHC-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_09409</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000171">
        <property type="protein sequence ID" value="OAV88528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G7J0"/>
    <dbReference type="EnsemblFungi" id="PTTG_09409-t43_2">
        <property type="protein sequence ID" value="PTTG_09409-t43_2-p1"/>
        <property type="gene ID" value="PTTG_09409"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09409"/>
    <dbReference type="OrthoDB" id="1111734at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033194">
        <property type="entry name" value="MFAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009730">
        <property type="entry name" value="MFAP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15327">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06991">
        <property type="entry name" value="MFAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="358"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="339"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="398"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="448"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OAV88528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09409-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="536" mass="62164" checksum="99F0A3DB5DB7739D" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G8L6</accession>
    <name>A0A180G8L6_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gem-associated protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_04736</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gemin-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000146">
        <property type="protein sequence ID" value="OAV88964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G8L6"/>
    <dbReference type="EnsemblFungi" id="PTTG_04736-t43_2">
        <property type="protein sequence ID" value="PTTG_04736-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04736"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04736"/>
    <dbReference type="OrthoDB" id="428895at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035426">
        <property type="entry name" value="Gemin2/Brr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12794:SF0">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12794">
        <property type="entry name" value="GEMIN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04938">
        <property type="entry name" value="SIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV88964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04736-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="28625" checksum="2F7E5243A071974D" modified="2016-09-07" version="1">MECLPMGPNIFLLREARARPRVVAKENPYKLPNRSLPPPARVECSRSGPSDTWRKKFLDRFMNMRQSLQAQEPWHWDLADDFRRPPPDNDESNWKIFISGKRTNLKDHPPRVPIPLFLKALEQATVIAVLNHYQTWIQERIKALATMVEHNSDTELQSNTEDAPESQEIILLSPSDGAWLIGLLAVLDAVLTSEDVFKLRELARSCKHVIRITNAALELTQDSSAEQEAGVAWMVIAAVADVWGQKDLWDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G938</accession>
    <name>A0A180G938_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">25S rRNA adenine-N(1) methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PTTG_12520</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(1) position of an adenine present in helix 65 in 25S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BMT2 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="ADAS02000136">
        <property type="protein sequence ID" value="OAV89226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G938"/>
    <dbReference type="EnsemblFungi" id="PTTG_12520-t43_1">
        <property type="protein sequence ID" value="PTTG_12520-t43_1-p1"/>
        <property type="gene ID" value="PTTG_12520"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12520"/>
    <dbReference type="OrthoDB" id="5954793at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016433">
        <property type="term" value="F:rRNA (adenine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03044">
        <property type="entry name" value="BMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021867">
        <property type="entry name" value="Bmt2/SAMTOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21008:SF1">
        <property type="entry name" value="25S RRNA (ADENINE(2142)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21008">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SENSOR UPSTREAM OF MTORC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11968">
        <property type="entry name" value="Bmt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OAV89226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12520-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="418" mass="47122" checksum="DF8934DEE55A0C3A" modified="2016-09-07" version="1">MVQANRRPGAKSKSTRNSLQAAILSKLTRTKQTKQLAITPSTSEPNANSQSTSSQTETLDRPSIDPTPEVSSSSLSSQSTSLEEIPAETDLKRKRLDPDKIEITSTVIKNKVKPSRQKINHPETEVEQEDVDQQENKQKLEQLIRKHSKKIKNDPSKLISEFHRIEKQLSQANLDPLTRHKLILAQKKLGGLEAYQTASKIGGAVEKGGETGKWCAQTLIDLDLRPSGPPATINKISLLDVGAIDGKSYAKYTDWISTTSIDINPIDESLVARYDFFKFPLPKSELQKFDVVCLSLVLNFIGDLKMRGEMIKRARRFLKPAGNLFIVLPLACLNNSRYLDFKRFRMILNYLGFKVLKQHNSSKLTYWFLEKEDHQSNTVDETQPDFTSPNHSKSASSLRFPKVEIRSGPSRNNFCIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G942</accession>
    <name>A0A180G942_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_12447</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000144">
        <property type="protein sequence ID" value="OAV89008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G942"/>
    <dbReference type="EnsemblFungi" id="PTTG_12447-t43_1">
        <property type="protein sequence ID" value="PTTG_12447-t43_1-p1"/>
        <property type="gene ID" value="PTTG_12447"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12447"/>
    <dbReference type="OrthoDB" id="2506480at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV89008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12447-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="524" mass="59887" checksum="6A6FE7A7DDB2B5BC" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G9P3</accession>
    <name>A0A180G9P3_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Queuosine 5'-phosphate N-glycosylase/hydrolase</fullName>
            <ecNumber evidence="1">3.2.2.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Queuosine-nucleotide N-glycosylase/hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_07774</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of queuosine 5'-phosphate, releasing the nucleobase queuine (q). Is required for salvage of queuine from exogenous queuosine (Q) that is imported and then converted to queuosine 5'-phosphate intracellularly.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>queuosine 5'-phosphate + H2O = queuine + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:75387"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17433"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:194371"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:75388"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the QNG1 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="1"/>
    <dbReference type="EMBL" id="ADAS02000136">
        <property type="protein sequence ID" value="OAV89219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G9P3"/>
    <dbReference type="EnsemblFungi" id="PTTG_07774-t43_1">
        <property type="protein sequence ID" value="PTTG_07774-t43_1-p1"/>
        <property type="gene ID" value="PTTG_07774"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_07774"/>
    <dbReference type="OrthoDB" id="416777at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019438">
        <property type="entry name" value="Q_salvage"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21314">
        <property type="entry name" value="QUEUOSINE 5'-PHOSPHATE N-GLYCOSYLASE_HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21314:SF1">
        <property type="entry name" value="QUEUOSINE SALVAGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV89219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_07774-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="302" mass="33959" checksum="C43F10294070C418" modified="2016-09-07" version="1">MYASRPGQIINGCPSVFSGDEDLFRGDLCQYVDCSPATTTNNSFAMADSRLEIAPLPAQDITIADAIKASCKECLERYGININKSACEDFLLKLDRDEYEKRVEAEPVHLRFPLQFSSQLDELNVVAMVCLLEGFHSYEDLLKEKTGRGVWETMRYLVLAAYLSPESQSTSILTTRGLVEATPEYISTVLSLPLHIERPIAENSPISLAEPDRAVARFVEDLLEVLHSTGKVLQQSGLPNLASFLATRTTLNEDEMIRQLTGAFSTFRDMYTLDGKCAFNEYFRIWLLTLVETIIPQCRFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GAA9</accession>
    <name>A0A180GAA9_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_12332</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000123">
        <property type="protein sequence ID" value="OAV89611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GAA9"/>
    <dbReference type="EnsemblFungi" id="PTTG_12332-t43_1">
        <property type="protein sequence ID" value="PTTG_12332-t43_1-p1"/>
        <property type="gene ID" value="PTTG_12332"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12332"/>
    <dbReference type="OrthoDB" id="77607at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV89611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12332-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="186" mass="21286" checksum="A49D4343D87FEA3D" modified="2016-09-07" version="1">MSQDDPVDGPTFQEKKVRFDGPSSREDTRESSNTQPPESSLPADFFDDPSQAPTKVGIQAEEEEDLEWKAFEAALQEEAGTKNSPANLFAQASVIAEPVLYESEKPSETRISQDPELEDSEMIEGEQEQEEDPIEKKIREEKEEIMDRIQNEEREQLEADQRVLRMKARVNAFRASRKRTPRNLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GAB0</accession>
    <name>A0A180GAB0_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_05252</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000122">
        <property type="protein sequence ID" value="OAV89627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GAB0"/>
    <dbReference type="EnsemblFungi" id="PTTG_05252-t43_2">
        <property type="protein sequence ID" value="PTTG_05252-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05252"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05252"/>
    <dbReference type="OrthoDB" id="2495534at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV89627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05252-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="129" mass="14415" checksum="27A83095F496207B" modified="2016-09-07" version="1">MDIAGGPHSVCDPERGYDTKKITGACLWTGQRQSPEFTKDGYYPGWVNGDHKENCYRKLWLKPDQGPVVYAPVIDGCAFANEGQTISEDDGCATIWVTRKLFTALGGKKDQHSVWIHSWDFEKHQGPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GBC5</accession>
    <name>A0A180GBC5_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Geranylgeranyl transferase type-2 subunit beta</fullName>
            <ecNumber evidence="8">2.5.1.60</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PTTG_08985</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="8"/>
    <dbReference type="EMBL" id="ADAS02000136">
        <property type="protein sequence ID" value="OAV89203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GBC5"/>
    <dbReference type="EnsemblFungi" id="PTTG_08985-t43_3">
        <property type="protein sequence ID" value="PTTG_08985-t43_3-p1"/>
        <property type="gene ID" value="PTTG_08985"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08985"/>
    <dbReference type="OrthoDB" id="5428259at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02894">
        <property type="entry name" value="GGTase-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026873">
        <property type="entry name" value="Ptb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF11">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="9">
        <location>
            <begin position="3"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV89203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08985-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="223" mass="24479" checksum="87DB1F8417C2DBBA" modified="2016-09-07" version="1">MQNAIDVLEKPKVIDYILSLFDPERGSFSGDSWGETDARFSYCAVSGLALLGGLERLDRERVVGFISRCQNFDGGFGMVEGAESHAAYVWTCVGTLAILGRLDMVDKNTLGWWLSERQVPNGGLNGRPEKLEDVCYSWWALASLVIIGKAEWIDRSKLTSFILSCQDPDSGGIADRPGDMPDVWHTVFGLAGLSMLGYEGLKPVDPVFCMPAQFTKGLVEENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GCB8</accession>
    <name>A0A180GCB8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_08353</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000107">
        <property type="protein sequence ID" value="OAV90179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GCB8"/>
    <dbReference type="EnsemblFungi" id="PTTG_08353-t43_1">
        <property type="protein sequence ID" value="PTTG_08353-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08353"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08353"/>
    <dbReference type="OrthoDB" id="2497382at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV90179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08353-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="345" mass="40321" checksum="5594DB3B83F5C402" modified="2016-09-07" version="1">MADALEPTRFEEVRFSDDPETEITWLQEIASDACEILSSVFTQQRQASIEPRRQSRAYYTLHPYYYPQIVWTVDQILIGIVDLFVIHMISLQDEEPWPVESGQAALGNLLRLIDQMISDLQHPRRMVQAKWQRVVREIELMELAVIKCSKPKELRENINPEGIDGRIDLQWIEWVPREEMDQSQADVLESFLPVLKICRILLNKLSRPTSSEPLVLLEDDMDILNGTYAQTEQMEQTLRMFTIAVLKLRPREARKRMADSFANFGYLLQKNQSYLRACECDNPDDSALVQKALAWCQRWTVQYQTAASNFKKKLASCYDAEFPYYDDSGDETERVISRRRFFGMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GCC1</accession>
    <name>A0A180GCC1_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CCHC-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_28423</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000108">
        <property type="protein sequence ID" value="OAV90108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GCC1"/>
    <dbReference type="EnsemblFungi" id="PTTG_28423-t43_1">
        <property type="protein sequence ID" value="PTTG_28423-t43_1-p1"/>
        <property type="gene ID" value="PTTG_28423"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_28423"/>
    <dbReference type="OrthoDB" id="2512852at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="342"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV90108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_28423-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="406" mass="44718" checksum="160E8894A089A507" modified="2016-09-07" version="1">MMSRRNSVNSHASGREEEEFVPDGGARMASLFDLFTGGNSGRGGDETIRPLNNPTGAQPGGSATGHLNVQNRGAPPHIPPPQQGAAGARPGNVVNDLSRAHQDFLAARLQLEQAKLVSQTAKLINDWWTDGKRLAANGANLTQWLKELEEIGRSNMSDGDFFTQPIQNTTFEKIARMVVLEGVHPSLVPDLQRAKSALEMLSILKKRFLVVSRAAQMNVWNRFLDFSVNDAEGAGLSSTMRNLYTEWNNLNVRIHSDAFFGFVFQQAVMQSLVAFKNDFLQWVKTSMQHDESKKTLADSSAPSVLLAEGGEEEVNFDALLADVPEENWPDVLDFYATTAHWCWQCGAADHYLSECPQRVKPNQINKKLRGPGVSPFPMPAHRTQATFVGSLYTQPTSAQSPNQSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GCG8</accession>
    <name>A0A180GCG8_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nodulin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PTTG_12602</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
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                <person name="Young S."/>
                <person name="Zeng Q."/>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
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            <strain evidence="8">Isolate 1-1 / race 1 (BBBD)</strain>
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                <person name="Feldgarden M."/>
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                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
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                <person name="Goldberg J."/>
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                <person name="Heilman E."/>
                <person name="Heiman D."/>
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                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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                <person name="Khalil H.B."/>
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                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
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                <person name="Goldberg J."/>
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                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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                <person name="Khalil H.B."/>
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                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
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                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GEJ3"/>
    <dbReference type="EnsemblFungi" id="PTTG_28082-t43_2">
        <property type="protein sequence ID" value="PTTG_28082-t43_2-p1"/>
        <property type="gene ID" value="PTTG_28082"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_28082"/>
    <dbReference type="OrthoDB" id="5525680at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052053">
        <property type="entry name" value="IM_YidH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187:SF2">
        <property type="entry name" value="DUF202 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187">
        <property type="entry name" value="FGR18P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV91146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_28082-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="104" mass="11635" checksum="B478E3B0D1B3FCCE" modified="2016-09-07" version="1">MDHEASTNLDLTSVKPDSPIHSWFSSPLIIPNDGSTARDHLGRDRTWMQWIRLSTLLVLVSLATCISFPSDSDEHDHGDPEDDKHSEEYLNPVQTKYSSSLGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GFG1</accession>
    <name>A0A180GFG1_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Flavoprotein domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_05102</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000080">
        <property type="protein sequence ID" value="OAV91456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GFG1"/>
    <dbReference type="EnsemblFungi" id="PTTG_05102-t43_3">
        <property type="protein sequence ID" value="PTTG_05102-t43_3-p1"/>
        <property type="gene ID" value="PTTG_05102"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05102"/>
    <dbReference type="OrthoDB" id="1532798at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="3">
        <location>
            <begin position="11"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV91456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05102-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="208" mass="23088" checksum="04BAF650A713B00F" modified="2016-09-07" version="1">MEEQSKPGKLNVLIGTSGSVATIKLPTIVQELSKYEMLEVQVVTTPSALHFFEPSQLVTAKGEPVKVWTDEEEWQSWKKIPDPIVHIELRRWADVILVCPCSANTLAKISNGLCDNLLTSLIRATNPKDTPVIIFPSMNTLMYEHPLTRKQIKIVAEEIGIEVNGPISKTLACGDKGLGAMTEWRDIVDLVVTRFLTKKMSIGNTCES</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GFG5</accession>
    <name>A0A180GFG5_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MFS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_04704</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000085">
        <property type="protein sequence ID" value="OAV91188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GFG5"/>
    <dbReference type="EnsemblFungi" id="PTTG_04704-t43_2">
        <property type="protein sequence ID" value="PTTG_04704-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04704"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04704"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000171">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF12">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G01740)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OAV91188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04704-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="473" mass="51416" checksum="1BA50130406C86B9" modified="2016-09-07" version="1">MQEIGMGRYQWKLFVVVGFGWAADNLWPIVTSLILVPVSQEFSAKQPAFLTLSQNLGLLFGALIWGFGCDIFGRKLGFNITIFTTAIFALLSASSTNFISICLCVFFWSTGVGGNLPVDSAIFLEFLPGSHQYLLTVLSIFWSIAQLLVTLLAWPLLSNFSCQPKAQSCTRGENAGWRWLLISVGSAAFLMFLARFFTIEIHESPKYLMGKGRPEEAVAVVQEVARRNGVKCSLTIAHLEQFGSIENVVEKSNELTSPKTLLMRTLRRFDFQHLSGLFNTPVLALSTGLITTIWALIGLAFPLYNAFIPLIETQRGIKFGDETSTTYRNSLIIAAVGLPGSAIGALMTQTPQLGRKGSLAITSALTGISLFGSLTAKSSSILLVWNCLFGLNTNFLLSILYSYTPEIFVTKNRGTGNALTASVGRICGTLAPVVFMCTNIKSSLPVYISGSLFFAVAFTSLLLPLESHGSASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GFH8</accession>
    <name>A0A180GFH8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_27945</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000080">
        <property type="protein sequence ID" value="OAV91440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GFH8"/>
    <dbReference type="EnsemblFungi" id="PTTG_27945-t43_1">
        <property type="protein sequence ID" value="PTTG_27945-t43_1-p1"/>
        <property type="gene ID" value="PTTG_27945"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_27945"/>
    <dbReference type="OrthoDB" id="2507080at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="412"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV91440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_27945-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="501" mass="55066" checksum="07D731FEFA4E1080" modified="2016-09-07" version="1">MNSLTASFTTILTPTPLIVNVPIKGFEFNMFSEKYVHLMANRQEGNSFPRPNSEQRRISRQGAAVIGPLELAAAYTSASRQLGYPGVINFLGTSFNSTTAGVVSAVAARKEDALDADSDRPLTLNFRDLKSPFKFVDPSFKPSKKFTFNFTTIQQGFTADVRCQALPQNPTAQQPGLSVLFDSNVTLPDQTLNSLWRYFVQCPSQGLPSAPSNKSILINPVGSMILSHTCFNTNFEGSSVLGSQLLILGGYGPMYSFITPRACEFTPYSTLVEVQYSNIANVSRIISREPITPATAAVASRMYGAVLDATEQYVVSFRNKFGDDICALYGISSQLESTNDPLLNTILEAYLRGLVETRASTTKTPPIASAPGHLLAASLQDLSPEWLRSFDGIWSYETLGWHNAYESIKSTVIGIVPILLITTTTIFLVLFSWLSLKGTRHLRDEPFDPNNLVTLLQAGRDGSVVDILGHRKNLDSALGRDVRIQLHRHATQWQLRPVKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GFJ1</accession>
    <name>A0A180GFJ1_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_27986</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="ADAS02000081">
        <property type="protein sequence ID" value="OAV91425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GFJ1"/>
    <dbReference type="EnsemblFungi" id="PTTG_27986-t43_3">
        <property type="protein sequence ID" value="PTTG_27986-t43_3-p1"/>
        <property type="gene ID" value="PTTG_27986"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_27986"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV91425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_27986-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="210" mass="24495" checksum="A6F66F8215E272DB" modified="2016-09-07" version="1">MVMDLLGCSLDRYLTARGKFSLKTVLMLADQMIVRLQQLHSAGFVHRDIKPGNFVMGRGSGSNVVHLIDFGLSCRYRCEDYQHIPYKEGQPLVGTACYSSICTHLGIQQTRRDDLEALCYMLISFLTGNLPWLGIRAETKLEKHQIMAEQKIRTPMEKLCKGLPEEFSYFLAYARALRYKDRPDYEHIRRMFKSLFLRKGYRMDFVFDWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GFJ4</accession>
    <name>A0A180GFJ4_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PTTG_08186</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PIAS family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000086">
        <property type="protein sequence ID" value="OAV91102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GFJ4"/>
    <dbReference type="EnsemblFungi" id="PTTG_08186-t43_3">
        <property type="protein sequence ID" value="PTTG_08186-t43_3-p1"/>
        <property type="gene ID" value="PTTG_08186"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08186"/>
    <dbReference type="OrthoDB" id="28127at2759"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.780">
        <property type="entry name" value="PINIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023321">
        <property type="entry name" value="PINIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038654">
        <property type="entry name" value="PINIT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF4">
        <property type="entry name" value="TONALLI, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14324">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51466">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PINIT" evidence="11">
        <location>
            <begin position="45"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="SP-RING-type" evidence="10">
        <location>
            <begin position="250"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="351"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="360"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OAV91102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08186-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="458" mass="50152" checksum="F9042AB95D39AD7D" modified="2016-09-07" version="1">MRSGFPIKQPSNHNQNYSANSGINPNPPSQGFPPKPHQVHPSYSHAPFNSRALPISFEKSPFYRVDSAASGVAICHRAAQNDRKSVPLSLILSADQRAKMSQTHMNAIGSQYQLRLFATSEAFYNHPSYATGPHTQSLPPALIEFPTTTDIKLNTCSITSNLKGIKKQPGTAPPPDLALVPGRNGSGQALDLKEGRPNQIEIAYSNSDKRFYFVIYLVEYFGVYGLMKNLKANRKRGHDQVLQEIIASSGDEDVMTSASEVTLKDPVVFSRIKTPIRSTRCKHLQCFDAEMFYTMMEQTPTWLCPVCNSKLKNDDIAVDEYFESILKAAPSSIDTVVIEADGKWHDEKFEHGTSLKKRASQSVMSGESGASGSRPSGGVGGAHKQGAPSGLHGHPDGSLHQKRKVTVLELESDDNAGENDDDDDDDDDQESSRAQPAKRHKAFGHAADCVIDLTLDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GFM1</accession>
    <name>A0A180GFM1_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Flavoprotein domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_05102</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000080">
        <property type="protein sequence ID" value="OAV91455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GFM1"/>
    <dbReference type="EnsemblFungi" id="PTTG_05102-t43_1">
        <property type="protein sequence ID" value="PTTG_05102-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05102"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05102"/>
    <dbReference type="OrthoDB" id="1532798at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="3">
        <location>
            <begin position="11"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV91455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05102-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="216" mass="24089" checksum="F6A51BFD8E0780B2" modified="2016-09-07" version="1">MEEQSKPGKLNVLIGTSGSVATIKLPTIVQELSKYEMLEVQVVTTPSALHFFEPSQLVTAKGEPVKVWTDEEEWQSWKKIPDPIVHIELRRWADVILVCPCSANTLAKISNGLCDNLLTSLIRATNPKDTPVIIFPSMNTLMYEHPLTRKQIKIVAEEIGIEVNGPISKTLACGDKGLGAMTEWRDIVDLVVTRFLTKKMSIGNTYLVESCRGQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GGI2</accession>
    <name>A0A180GGI2_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PRELI/MSF1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_02666</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000073">
        <property type="protein sequence ID" value="OAV91857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GGI2"/>
    <dbReference type="EnsemblFungi" id="PTTG_02666-t43_2">
        <property type="protein sequence ID" value="PTTG_02666-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02666"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02666"/>
    <dbReference type="OrthoDB" id="407630at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV91857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02666-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="215" mass="24028" checksum="519B0A4D345C7BBD" modified="2016-09-07" version="1">MVVFTAQHLFPFAFRDVAIGIWHKYPNEHSTHITSVDVLDRSFLPDGTIRTERLISIRQHAPRWIMKLVGGAEEQYVREVIFYKVPSSADQSPMVLMGSVNLSMSSILVCREQIRYQPFHPQASNFVQRADIQAQGTLAIGESWGILGSKLESWARDRFATNAESGRLGFNNVLTTLFQNSQGRGSSGGEDSRQRSSNGPSNTSLTNDSEKPKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GGL7</accession>
    <name>A0A180GGL7_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_03750</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000075">
        <property type="protein sequence ID" value="OAV91771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GGL7"/>
    <dbReference type="EnsemblFungi" id="PTTG_03750-t43_2">
        <property type="protein sequence ID" value="PTTG_03750-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03750"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03750"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF479">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008109899" evidence="6">
        <location>
            <begin position="26"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV91771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03750-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="482" mass="52738" checksum="5A29DAF768917B02" modified="2016-09-07" version="1">MDSPAHTRLSFFFLLLSVLIVQLRAATPPGTKPSTPSGSGHLVFKSPLARQYAVPERLPTVPFPIQPSYAGRLDASPTPKQASKIFFWFVPADDARGSNDLTIWINGDDCSGLIGAFNGNGPITMRGKTAVKNPNSWTRSSSMLYLDAPLNAGYSTGPVKQKGSADIGTLVFHFLVSFLKVFPEMKGKNLYLAGQAYSGTYVSYAADKIYAQQSMLALKLQGTLLVNAFISTAELQQHIPAASYVTKNNDVFQFDPKMLKDLQATDNACGLTEYLTQNLKYPPRGALPPVTKAIDPKQAPLFRDKCLIYYDMYHGTFEDFNLWNIHAKRGTSDPNYTKFTYPGQPEFQKAFHVDGARRWHSCSNMDSVFPNGDASPPATSKVLPNVIERSKRTVIAHGSLDGSLPVDGIKLAIQGMTWGGKQGFQTPINSNFLVSGKPSGKFHTERGLTFVEVTQSGSMMPQDQGEAALALLEFLLGHRPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GHL0</accession>
    <name>A0A180GHL0_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_03701</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000068">
        <property type="protein sequence ID" value="OAV92165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="PTTG_03701-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03701"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03701"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV92165.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03701-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="377" mass="40555" checksum="B07A3768559C601E" modified="2016-09-07" version="1" fragment="single">MTQNNKFRSGIRLVRLVAVIFLVNGCKPMYLPNSQLSAEWFAWDEDLVDLDHIFPSGGSSGDEAAGQADTHHEPFAGGHLHPIGLPAGPSASHVCPPQDYHHIQTAAEQRPSGTLAHGKRMSLDLHNLFGSGFEPAQNFELLDQLLADGTPASNGFAAAGASRALDHIFPSALSSQARAAGQANHPQPSSALASDQSLLPDYYYPAQTNAKERTVNSQPYHALDISFDSGELFEPGLLPHDFDILTEFLGHGTPDPDGLTGVRAAQSHHGVAHVSDSHSHTFTGPGPSNLPAFSDYPFMRSSGTTSGLHESPSRKRKQTVGLQAKSIKPQDLLFTQSKPRSGIPKLYIPFPESQRPALSKKLPPQGPLGYYMNDLAL</sequence>
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        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
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                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
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                <person name="White J."/>
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                <person name="Izard J."/>
                <person name="Baranova O.V."/>
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                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
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    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
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    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
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        <property type="gene ID" value="PTTG_12081"/>
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    <dbReference type="OrthoDB" id="2499762at2759"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EMBL" id="OAV91989.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12081-t43_2-p1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="317" mass="35720" checksum="7E56AAA8780B569B" modified="2016-09-07" version="1">MRFATPPPPPMWCRSSFPISPHQSRRPPKPGWPHWRVSIPAGPNRVQPDHQGPALDDRSTRYRRTAAPSACAVRTLLLNLYPNVDRSKSDAMSQTAYQETLTRQLQLSKELYLDPGFFSSLSNLRSIHLAMIKLSISSFTFLLSSLAPSLSELGLHDVYFIGAEDTVPIFRAIFKLPHLHSLSLTGRLVSGRCIIPMLLYPSNYFDSGKSLETLSLCDEGLTSAGELMLTPWDLVQCLENLLPDGRSHTDDHHDSSQAKYPSPLKIANYRFCMSDPEWHQPFRALYVSARAELLGIRVEVAHHTDPLQRIKWMSSTS</sequence>
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<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GHR0</accession>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
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                <person name="Saif S."/>
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                <person name="Shenoy N."/>
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                <person name="Sykes S."/>
                <person name="Thomson T."/>
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                <person name="White J."/>
                <person name="Yandava C."/>
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                <person name="Haas B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">1-1 BBBD Race 1</strain>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
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    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
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            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
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    <dbReference type="EMBL" id="ADAS02000071">
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="ARF3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28245:SF1">
        <property type="entry name" value="ARF3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08616">
        <property type="entry name" value="SPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="529"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="718"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="529"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="729"/>
            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV92009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03586-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="799" mass="88749" checksum="C278C98A41A76B06" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GHR2</accession>
    <name>A0A180GHR2_PUCT1</name>
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        <recommendedName>
            <fullName evidence="3">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_05080</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000080">
        <property type="protein sequence ID" value="OAV91493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GHR2"/>
    <dbReference type="EnsemblFungi" id="PTTG_05080-t43_1">
        <property type="protein sequence ID" value="PTTG_05080-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05080"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05080"/>
    <dbReference type="OrthoDB" id="25002at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="64"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="257"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="343"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="273"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="409"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="463"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="512"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV91493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05080-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="538" mass="59395" checksum="F1198DE646DC093C" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GI37</accession>
    <name>A0A180GI37_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_09890</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000077">
        <property type="protein sequence ID" value="OAV91623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GI37"/>
    <dbReference type="EnsemblFungi" id="PTTG_09890-t43_2">
        <property type="protein sequence ID" value="PTTG_09890-t43_2-p1"/>
        <property type="gene ID" value="PTTG_09890"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09890"/>
    <dbReference type="OrthoDB" id="3341596at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051714">
        <property type="entry name" value="Znf_CCHC_NABP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23002">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <source>
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        <source>
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            <dbReference type="EnsemblFungi" id="PTTG_09890-t43_2-p1"/>
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        <source>
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</entry>
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    <name>A0A180GIR1_PUCT1</name>
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        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000074">
        <property type="protein sequence ID" value="OAV91833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="PTTG_27865"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_27865"/>
    <dbReference type="OrthoDB" id="3357224at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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</entry>
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    <accession>A0A180GIS2</accession>
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    <reference evidence="2" key="1">
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
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                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000062">
        <property type="protein sequence ID" value="OAV92545.1"/>
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        <property type="protein sequence ID" value="PTTG_08636-t43_2-p1"/>
        <property type="gene ID" value="PTTG_08636"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08636"/>
    <dbReference type="OrthoDB" id="2502260at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
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        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV92545.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08636-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="204" mass="22947" checksum="A13B00B6C666FAE9" modified="2016-09-07" version="1">MGHLRRFSESSVRDKYESYPANCYRLWVTKHPSGARPARMTRRSFDFPLRAGTGGLKSVPMGSFLSPTVEEEEMSGSGDEPGLADDKNGDKLHAPECRTRFHRHQGPLRREQVLAPSCEPGNPHPRGTAGLKILIKRLSLSLLKTRSFVRTRSQPELSMVHSFDHPGPSRVDRQALVFGDENPEKIDERSGEFYKTFFPSLVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GJ25</accession>
    <name>A0A180GJ25_PUCT1</name>
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        <submittedName>
            <fullName evidence="10">BZIP domain-containing protein</fullName>
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        <name evidence="9" type="ORF">PTTG_03490</name>
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    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
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    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
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                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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            <strain evidence="9">1-1 BBBD Race 1</strain>
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        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">1-1 BBBD Race 1</strain>
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        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
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            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
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            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
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                <person name="Earl A."/>
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                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
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                <person name="Goldberg J."/>
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                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
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                <person name="Larson L."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
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                <person name="Wang M."/>
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                <person name="Szabo L.J."/>
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    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_08315</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000054">
        <property type="protein sequence ID" value="OAV93157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GK57"/>
    <dbReference type="EnsemblFungi" id="PTTG_08315-t43_2">
        <property type="protein sequence ID" value="PTTG_08315-t43_2-p1"/>
        <property type="gene ID" value="PTTG_08315"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08315"/>
    <dbReference type="OrthoDB" id="4605274at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053228">
        <property type="entry name" value="Stereospecific_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37574">
        <property type="entry name" value="LIPASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37574:SF1">
        <property type="entry name" value="LIPASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008109988" description="Lipase" evidence="2">
        <location>
            <begin position="19"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="110"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV93157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08315-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="434" mass="45575" checksum="FA884F37FE0E031F" modified="2016-09-07" version="1">MIAPVAVVLFLLVLHARATEPAELEKENKGQADLSKRGLLPSLLGGSPGRGQSGIGGLLGGSPSHGYGGGPQSIGSGLVDGVTGLLPLGPQSPGGSLNDLLNGVPKYDQAATTAGPRPPTARGDAPWSQSERTYRQQITCLGHTQGQRGIVLLVPPTGGNGQQVWPRSPYAQLPRYGFSICWVDNLSKSTGDAQLTAEFIAYAIIHLAAQSRQPLNVISYSQGGMDAQWAFTFWPSTRRLVTNFIALASPFHGTYAANVVCPLLEVAGGCLPAVWQMMVNSRFQQALNARAPNSGARALVPTTSIYSYDDEIVLPQVGGRPISALDGASNIAVQDVCGAQHFADHFLIVGDHGAFGIALNALMSGRPANRANIDKSYCNQLAGLGGQLGSLGNDLKYAFATVVGNTGDRINSLLKTLTTLVSPELGIEKLDRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GKT6</accession>
    <name>A0A180GKT6_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial inner membrane protease subunit</fullName>
            <ecNumber evidence="8">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PTTG_01579</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase S26 family. IMP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="8"/>
    <dbReference type="EMBL" id="ADAS02000051">
        <property type="protein sequence ID" value="OAV93406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="ADAS02000051">
        <property type="protein sequence ID" value="OAV93407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GKT6"/>
    <dbReference type="EnsemblFungi" id="PTTG_01579-t43_1">
        <property type="protein sequence ID" value="PTTG_01579-t43_1-p1"/>
        <property type="gene ID" value="PTTG_01579"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_01579-t43_2">
        <property type="protein sequence ID" value="PTTG_01579-t43_2-p1"/>
        <property type="gene ID" value="PTTG_01579"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_01579"/>
    <dbReference type="OrthoDB" id="308440at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052064">
        <property type="entry name" value="Mito_IMP1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019757">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Lys-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12383:SF16">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12383">
        <property type="entry name" value="PROTEASE FAMILY S26 MITOCHONDRIAL INNER MEMBRANE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00760">
        <property type="entry name" value="SPASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S26" evidence="9">
        <location>
            <begin position="37"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV93406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_01579-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="198" mass="21778" checksum="C134716CB019E436" modified="2016-09-07" version="1">MSGASTFKTTLHLIKRAVQVTAGVTLFKDKVGDLRWCEGGSMLPTLNVTGDLLLQIPLSSVFNFSLRRDRETEKSSQALRMSEPTTSRLNLNRGDLVNFTSPLNPSVLACKRILGLPGDKILVDDHPHSFNPTPDLLNTPGYDYQYSRKSLLTIPEGHLWLQGDNYAVSIDSRTYGPVPIGLVSGKIVARVSVLFLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GKV8</accession>
    <name>A0A180GKV8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="10">tRNA-synt_1c domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_08026</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000055">
        <property type="protein sequence ID" value="OAV93099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GKV8"/>
    <dbReference type="EnsemblFungi" id="PTTG_08026-t43_1">
        <property type="protein sequence ID" value="PTTG_08026-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08026"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08026"/>
    <dbReference type="OrthoDB" id="428822at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049940">
        <property type="entry name" value="GluQ/Sye"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311:SF2">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="An anticodon-binding domain of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="8">
        <location>
            <begin position="10"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OAV93099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08026-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="336" mass="37639" checksum="CE9D5B920F47CCDA" modified="2016-09-07" version="1">MRQGLRSSGSNLTYDRKCLQMSEAEVAEKLSTGTPHVVRLKNSDAPVYVDDLVYGQLTFTNNTQDDVILIKADGTPTYHFANVVDDHHMGISHVLRGEEWLSSTPKHILLYNAFGFPLPKFAHLPLLVNADGSKLSKRSGDVRVEDYISKGYEPEALLNFVALMGMNHSRHGADEGKTGDKTTDVMTMEQMISAFSIDVIGKHRSTMSQPKLIHLNQQHIARCLLSTDPAQVQPWIARARSILLSGPGKPEVSDAYVHRVLLAMKDRLHVFTDIKYLAEYFFTLPDLTSSEACEMRKNVPDVLYRACFPFAASEILRSFCHFKAGGGGFSFWWMVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GKX8</accession>
    <name>A0A180GKX8_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Abasic site processing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_01603</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SOS response-associated peptidase family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000051">
        <property type="protein sequence ID" value="OAV93446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GKX8"/>
    <dbReference type="EnsemblFungi" id="PTTG_01603-t43_3">
        <property type="protein sequence ID" value="PTTG_01603-t43_3-p1"/>
        <property type="gene ID" value="PTTG_01603"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_01603"/>
    <dbReference type="OrthoDB" id="2111841at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106300">
        <property type="term" value="P:protein-DNA covalent cross-linking repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1680.10">
        <property type="entry name" value="SOS response associated peptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003738">
        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036590">
        <property type="entry name" value="SRAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604:SF0">
        <property type="entry name" value="ABASIC SITE PROCESSING PROTEIN HMCES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604">
        <property type="entry name" value="DC12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02586">
        <property type="entry name" value="SRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143081">
        <property type="entry name" value="BB1717-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OAV93446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_01603-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="27838" checksum="A30DC34FECA75EB6" modified="2016-09-07" version="1" fragment="single">MCGRFALSLKPEEISSRLTDIQLHVDSWLNVDEYRPNYNLAPQNRAAVVRRMDPKENRLVIDCMKWGLVPHWSESPPTHGSLLNTINARDDKVVEPRGLWNTIKGKKRCIILCEGFFEWLTKGKDKIPHFTKRTGGQLMCLAGLWESVTYKGATEELHTFTIITTSSNKYLSFLHDRMPVILTDPESIETWLDTSSGEWSGNLAKLLKPFDLSDGLVCYPVPKEVGKVGNQSADFLKIQRFLVCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GL75</accession>
    <name>A0A180GL75_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_00904</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000052">
        <property type="protein sequence ID" value="OAV93294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GL75"/>
    <dbReference type="EnsemblFungi" id="PTTG_00904-t43_2">
        <property type="protein sequence ID" value="PTTG_00904-t43_2-p1"/>
        <property type="gene ID" value="PTTG_00904"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00904"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV93294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00904-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="287" mass="31030" checksum="B017EF9B777F0E85" modified="2016-09-07" version="1">MVELNNKPVRGKRILVTQANESQYQEHQAANQSKPKHPHQNNSNHQGRPGQRGRPVGPDPMRPTTLSILKSQQQPKGVNNKIAALEAKLASMQRPGISSATNPSSSNSSLPSTSSLLSSKPSFLPEHPNTLRAGAHKTTSSMRSCNPITTTSLRSSKNSTAPYSNLKNPPNWDAIGLTLKEQLKRTRELQSIPTKAAKPAPTADHQSDLPPSSTSPRPKKRRSYENSSKALGQSDSNLANTDGSNEVSAQFTEKACDTENGFKRQKLDAFSALSEPSEASTLPEPVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GMV7</accession>
    <name>A0A180GMV7_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribonuclease Z</fullName>
            <ecNumber evidence="4">3.1.26.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PTTG_08554</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="16">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase Z family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="4"/>
    <dbReference type="EMBL" id="ADAS02000052">
        <property type="protein sequence ID" value="OAV93263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GMV7"/>
    <dbReference type="EnsemblFungi" id="PTTG_08554-t43_1">
        <property type="protein sequence ID" value="PTTG_08554-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08554"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08554"/>
    <dbReference type="OrthoDB" id="527344at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990180">
        <property type="term" value="P:mitochondrial tRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07718">
        <property type="entry name" value="RNaseZ_ELAC1_ELAC2-C-term-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047151">
        <property type="entry name" value="RNZ2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027794">
        <property type="entry name" value="tRNase_Z_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553:SF49">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13691">
        <property type="entry name" value="Lactamase_B_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNase Z endonuclease" evidence="13">
        <location>
            <begin position="18"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="12">
        <location>
            <begin position="524"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="142"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OAV93263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08554-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="669" mass="73561" checksum="5FEFF0D49ACB9096" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GMZ8</accession>
    <name>A0A180GMZ8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_06744</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000044">
        <property type="protein sequence ID" value="OAV94045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GMZ8"/>
    <dbReference type="EnsemblFungi" id="PTTG_06744-t43_2">
        <property type="protein sequence ID" value="PTTG_06744-t43_2-p1"/>
        <property type="gene ID" value="PTTG_06744"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06744"/>
    <dbReference type="OrthoDB" id="2499436at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV94045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06744-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="99" mass="10805" checksum="04718D5720AD8D67" modified="2016-09-07" version="1">MEAHDHGAHFNHTQPAADHHPGLAGNTQGANIQNEASRPDENVIGSITKVDETQTRVPFKAKVEGYAKKIAGKTFGKEHEVAAGEARLRGEDQPKHYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GNZ8</accession>
    <name>A0A180GNZ8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Autophagy-related protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_06736</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000044">
        <property type="protein sequence ID" value="OAV94032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GNZ8"/>
    <dbReference type="EnsemblFungi" id="PTTG_06736-t43_1">
        <property type="protein sequence ID" value="PTTG_06736-t43_1-p1"/>
        <property type="gene ID" value="PTTG_06736"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06736"/>
    <dbReference type="OrthoDB" id="20368at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043548">
        <property type="term" value="F:phosphatidylinositol 3-kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.40">
        <property type="entry name" value="Autophagy protein 6/Beclin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007243">
        <property type="entry name" value="Atg6/Beclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038274">
        <property type="entry name" value="Atg6/Beclin_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041691">
        <property type="entry name" value="Atg6/beclin_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12768">
        <property type="entry name" value="BECLIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12768:SF4">
        <property type="entry name" value="BECLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17675">
        <property type="entry name" value="APG6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Atg6/beclin coiled-coil" evidence="3">
        <location>
            <begin position="132"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="188"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV94032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06736-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="318" mass="36187" checksum="13A0BAD5E178B71B" modified="2016-09-07" version="1">MFGNIGEDGHCQRCKQPLRIDDELAPSAYDLIASLSDRGGTPQPTPQRARNQPHTRANRLPTPPPSRDQCTHPGPAESFVVLSESTLGAARAQPDLRHEPPQSHGHPCPKTTDRVFKLAELLSGNPPKFKHPLCTECTDKLLEWMHNQLQSAKSNRDRYAIFERELNREKSAAAEGSYESCKKIKQDIEELKIRESVAIEKLRETETECEKVEAELREIERLESKQDKEEEQFWDDYNQYLLEYEDIQSQLDSLTTRYRNDQNELAKLRATNVYKDAFCIGCETGVGTINGLRLGRLTDVPIPLDPHGLLLPHRTHQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GQA4</accession>
    <name>A0A180GQA4_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Arf-GAP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_08830</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000039">
        <property type="protein sequence ID" value="OAV94462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GQA4"/>
    <dbReference type="EnsemblFungi" id="PTTG_08830-t43_2">
        <property type="protein sequence ID" value="PTTG_08830-t43_2-p1"/>
        <property type="gene ID" value="PTTG_08830"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08830"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08839">
        <property type="entry name" value="ArfGap_SMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000010">
        <property type="entry name" value="Stromal membrane-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051718">
        <property type="entry name" value="ARF_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044732">
        <property type="entry name" value="ArfGAP_SMAP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705:SF14">
        <property type="entry name" value="ARF-GAP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="3">
        <location>
            <begin position="17"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="151"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="189"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="236"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV94462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08830-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="248" mass="27523" checksum="49E90F3013B6B83A" modified="2016-09-07" version="1">MATRSARDNKALNARHAEVLKTMLRQPENKLCADCKRNDPRWASTNLGCFMCIRCSGIHRSMGVHITRIKSIDLDTWTPEQVACVQRWGNKRANAYWEAHLRPGHMPPDHKIESFIRSKYESKRWAMEGPVPEPETLDGDEHVAPAAPAPVAQKAVPSNPSITKVTPPTMSKPATSVPMTDNSMDFFGGPTEQPAKPASGAANAKTLRQLQSLPRPLLEGKMSRTTSFHFLRPNPNKATQVQSARPQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GQC4</accession>
    <name>A0A180GQC4_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_00787</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000035">
        <property type="protein sequence ID" value="OAV94895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GQC4"/>
    <dbReference type="EnsemblFungi" id="PTTG_00787-t43_2">
        <property type="protein sequence ID" value="PTTG_00787-t43_2-p1"/>
        <property type="gene ID" value="PTTG_00787"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00787"/>
    <dbReference type="OrthoDB" id="2505307at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV94895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00787-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="290" mass="29352" checksum="5F0475A8F388A437" modified="2016-09-07" version="1">MLLRKQPLPEIPTSPVDELPAGGVSSGGLLPSSRGSSTRKNTGPAYGLPAGANISESNILPLPTSKQSGGAPLIQAGSSDSPTQPTPAVRGSASGVPDANLSTNLPPNKNGTALQAPGDLTPARPSGTDQARINGSVTPTGSSSNLTSNILPSNSSSLPPKKTPTSPTSAEHSQGKTGKPTEDQKTAIVVSSVLLGALLILGVALAITRRKLNKREELKRQEEGTLQATPGELGKRGTVMVGRGLESSHGLTSSGGWLDSRSSEFGATRTLSVSSSSTSADSIEAELNPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GQC9</accession>
    <name>A0A180GQC9_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fungal-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_06941</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">1-1 BBBD Race 1</strain>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
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            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="EnsemblFungi"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
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        <property type="gene ID" value="PTTG_06941"/>
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    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06941-t43_2-p1"/>
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            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="253" mass="28466" checksum="9671CF099D5DCEFF" modified="2016-09-07" version="1">MKLLRTHSLPFEKIVKCRANLTPSFSKMEWNHAIQLWPQYKVITDIPEYNPIEPYIPKTFAAEVFRAIEKAEVDTSQSGAADNEARVAVLIRVILVEISSLFSGAVSNDAEGYIAGTASSQGRIESIYTVLGACVNVVLEVKAGGIRNRNFAQMMAELESCDMLNVSRQLNLNTLQGVLSSQTEWHFWAYDRRGFTVSEAIAPYHRDEKQEIMNRLKIVARLFGQFLKGYCPALHAFIRRSEGKASLNHSSQH</sequence>
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    <accession>A0A180GRC1</accession>
    <name>A0A180GRC1_PUCT1</name>
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        <submittedName>
            <fullName evidence="12">HSF_DOMAIN domain-containing protein</fullName>
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        <name evidence="11" type="ORF">PTTG_05194</name>
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    <organism evidence="11">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Homotrimer. Homotrimerization increases the affinity of HSF1 to DNA. Interacts with transcriptional coregulator SSA1 on chromatin.</text>
    </comment>
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            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the HSF family.</text>
    </comment>
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        <property type="protein sequence ID" value="OAV95074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblFungi" id="PTTG_05194-t43_4">
        <property type="protein sequence ID" value="PTTG_05194-t43_4-p1"/>
        <property type="gene ID" value="PTTG_05194"/>
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    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
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        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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            <end position="97"/>
        </location>
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        </location>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
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    </evidence>
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    </evidence>
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            <dbReference type="EMBL" id="OAV95074.1"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05194-t43_4-p1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GTC4</accession>
    <name>A0A180GTC4_PUCT1</name>
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        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
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    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_02272</name>
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    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
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                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
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                <person name="Khalil H.B."/>
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                <person name="Levin J.Z."/>
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                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GTD6"/>
    <dbReference type="EnsemblFungi" id="PTTG_11924-t43_2">
        <property type="protein sequence ID" value="PTTG_11924-t43_2-p1"/>
        <property type="gene ID" value="PTTG_11924"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_11924"/>
    <dbReference type="OrthoDB" id="2500360at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051857">
        <property type="entry name" value="Asn_synthetase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937:SF1">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asparagine synthetase" evidence="5">
        <location>
            <begin position="424"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="564"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OAV96015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11924-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="574" mass="63466" checksum="C561FCC4E09151D0" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GTF4</accession>
    <name>A0A180GTF4_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lactoylglutathione lyase</fullName>
            <ecNumber evidence="3">4.4.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Aldoketomutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Ketone-aldehyde mutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Methylglyoxalase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">S-D-lactoylglutathione methylglyoxal lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PTTG_02109</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="15">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit. In the homodimer, two zinc ions are bound between subunits.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glyoxalase I family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.5" evidence="3"/>
    <dbReference type="EMBL" id="ADAS02000022">
        <property type="protein sequence ID" value="OAV96087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GTF4"/>
    <dbReference type="EnsemblFungi" id="PTTG_02109-t43_1">
        <property type="protein sequence ID" value="PTTG_02109-t43_1-p1"/>
        <property type="gene ID" value="PTTG_02109"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02109"/>
    <dbReference type="OrthoDB" id="16820at2759"/>
    <dbReference type="UniPathway" id="UPA00619">
        <property type="reaction ID" value="UER00675"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07233">
        <property type="entry name" value="GlxI_Zn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004361">
        <property type="entry name" value="Glyoxalase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018146">
        <property type="entry name" value="Glyoxalase_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00068">
        <property type="entry name" value="glyox_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374:SF30">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE GLYOXALASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00934">
        <property type="entry name" value="GLYOXALASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="VOC" evidence="13">
        <location>
            <begin position="11"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604361-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR604361-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OAV96087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02109-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="157" mass="18039" checksum="C35EF682385BEBF6" modified="2016-09-07" version="1">MSHIASQQTFKLNHTMFRIKDPKISLEFYQDVLGMKLLRKMEVESAKFTNYFLGFQDSNQDLKSASPLHREGIVELCHNWGTESDDNFSGYHNGNKSPQGFGHLAITCDDVEKTCAYLEEKQVKFQKKLNDGSMKGIAFIQDPDGYWIEILGNNFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GTG9</accession>
    <name>A0A180GTG9_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Methylenetetrahydrofolate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PTTG_04810</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000026">
        <property type="protein sequence ID" value="OAV95679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GTG9"/>
    <dbReference type="EnsemblFungi" id="PTTG_04810-t43_2">
        <property type="protein sequence ID" value="PTTG_04810-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04810"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04810"/>
    <dbReference type="OrthoDB" id="16284at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:methylenetetrahydrofolate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.220:FF:000002">
        <property type="entry name" value="Methylenetetrahydrofolate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004621">
        <property type="entry name" value="Fadh2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053806">
        <property type="entry name" value="MTHFR_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00677">
        <property type="entry name" value="fadh2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754:SF3">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD-linked oxidoreductase"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MTHFR SAM-binding regulatory" evidence="9">
        <location>
            <begin position="302"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU004254"/>
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        <source>
            <dbReference type="Pfam" id="PF21895"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV95679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04810-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="640" mass="72346" checksum="24E7A941B438C539" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GTI5</accession>
    <name>A0A180GTI5_PUCT1</name>
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        <recommendedName>
            <fullName evidence="4">Uracil catabolism protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_02141</name>
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    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
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    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000022">
        <property type="protein sequence ID" value="OAV96117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="PTTG_02141-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02141"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02141"/>
    <dbReference type="OrthoDB" id="2153176at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
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    </dbReference>
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            <dbReference type="EMBL" id="OAV96117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02141-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="48569" checksum="69210708A955A035" modified="2016-09-07" version="1">MTSPPKGTDYLKSLGSIRERCQQVFNLTVDQDQLEFWSVDQSTLPSIVDFCSYLISRDYGANYQSIPPHGRWRHFLANERDRISPLLEQWSADSSVDPVETGRRLVDLFVVAVLMDAGAGDEWKFVEPLEESCSTSANANESLEQGIGRSEGLAVAEFMQSKAGNQMVGLEGRSNLLQKLGCLLSSGSKYFPAQEPNSQSRPGNLIDYILTHPDVHPLPRAVMRGEISMSIDVLWEVIIDEKDGLGSIWPKEGRQPIGGKFIGDVWTCEALNRVTKDGGHVPFHKLSQWLTYSLIEVLEKTLKWVITGSEKMTGLPEYRNGGLLIDFQYIQPKPMAFIKSLGLPEADSSSFSLMDTLPPLAASHPAIVEWRALTVIALDRIKDSINEKLELVGSAQALSLPQVLEAATWKGGREIARIKRAQDGSSAGPPIKVISDGTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GUG5</accession>
    <name>A0A180GUG5_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prolyl 4-hydroxylase alpha subunit Fe(2+) 2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_26514</name>
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    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GUG5"/>
    <dbReference type="EnsemblFungi" id="PTTG_26514-t43_2">
        <property type="protein sequence ID" value="PTTG_26514-t43_2-p1"/>
        <property type="gene ID" value="PTTG_26514"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_26514"/>
    <dbReference type="OrthoDB" id="124582at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099:SF7">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_26514-t43_2-p1"/>
        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="761" mass="85149" checksum="9CF5D4CB67A37164" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GUH9</accession>
    <name>A0A180GUH9_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_26387</name>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000020">
        <property type="protein sequence ID" value="OAV96420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GUH9"/>
    <dbReference type="EnsemblFungi" id="PTTG_26387-t43_2">
        <property type="protein sequence ID" value="PTTG_26387-t43_2-p1"/>
        <property type="gene ID" value="PTTG_26387"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_26387"/>
    <dbReference type="OrthoDB" id="2507296at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV96420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_26387-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="232" mass="26186" checksum="99B6F9BA4D7BA6E6" modified="2016-09-07" version="1">MPDVSPPKPSSSQDKQSEDTAKNLDPKSPPDYVVNAQALVKPPAPAMKNLSRPPDQVERRIAVNPIIKREKAAKEIPPTSKSKNKTPEDIRKSYPLRNTASHKTPTQQTLHEIMTMSIEELELRHRHALKKLQQRSVENQSAGTTDKDRLRKKYEQEIDLLRIKMKLDETDGRSGARGSHDKRINSSSLSPSDFKPAHGVPQQGSSKKVDSGRQEIPSPFSNQSRQKTWLEY</sequence>
</entry>
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    <accession>A0A180GUK5</accession>
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            <fullName evidence="6">Mitochondrial outer membrane protein IML2</fullName>
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        <name evidence="3" type="ORF">PTTG_03220</name>
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    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
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        <lineage>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000023">
        <property type="protein sequence ID" value="OAV95982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GUK5"/>
    <dbReference type="EnsemblFungi" id="PTTG_03220-t43_2">
        <property type="protein sequence ID" value="PTTG_03220-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03220"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03220"/>
    <dbReference type="OrthoDB" id="2154985at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005634">
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39C"/>
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        <property type="entry name" value="Iml2-TPR_39"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV95982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03220-t43_2-p1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
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    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GVI5</accession>
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        <recommendedName>
            <fullName evidence="11">TOG domain-containing protein</fullName>
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    <gene>
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    <organism evidence="12">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
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    <reference evidence="12" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLASP family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000017">
        <property type="protein sequence ID" value="OAV96805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GVI5"/>
    <dbReference type="EnsemblFungi" id="PTTG_03543-t43_2">
        <property type="protein sequence ID" value="PTTG_03543-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03543"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03543"/>
    <dbReference type="OrthoDB" id="46159at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031110">
        <property type="term" value="P:regulation of microtubule polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902903">
        <property type="term" value="P:regulation of supramolecular fiber organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF9">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="11">
        <location>
            <begin position="2"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="184"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="257"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="498"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="506"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="578"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="985"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OAV96805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03543-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="985" mass="107079" checksum="EA9E761A219E12FC" modified="2016-09-07" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GVN3</accession>
    <name>A0A180GVN3_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_01002</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000019">
        <property type="protein sequence ID" value="OAV96564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GVN3"/>
    <dbReference type="EnsemblFungi" id="PTTG_01002-t43_2">
        <property type="protein sequence ID" value="PTTG_01002-t43_2-p1"/>
        <property type="gene ID" value="PTTG_01002"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_01002"/>
    <dbReference type="OrthoDB" id="2495654at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051654">
        <property type="entry name" value="Meroterpenoid_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897:SF1">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OAV96564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_01002-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="39771" checksum="603E9B032D90F1EE" modified="2016-09-07" version="1">MSGFFFDYILYCFVPIISSVSSTLSYSTDTIFPMNSSEKDIFLPCAPSSPPDNCGIEKDDVSLPLKGVQAVYPLDESIWANDLEFFKDATGIRDAAALKKHIMKVRETAYAKFPYPCIWAFEFLKGKTTSHPFYTHVKDKHHSDQHQKFFIDLGTFVGVDIRQVVKDGWNPNNVLGIDIHGEWRDLGHQLFNDRNEALPFYLGNILEVDTLNNEPSVEPEARPPLELRGLKNLNQLKGRVSFISANQLFHLFSKEQQVELAKRCGQLLSHESGAAIFGTQLGLPEEKLEVYKLYLHSPTSWEKMWKDVLGEKIQVVVRLEKAEDTHPYRSFESVQDVYWLYWSVARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GWK5</accession>
    <name>A0A180GWK5_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="13">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PTTG_08937</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="19 20" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="20">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="13"/>
    <dbReference type="EMBL" id="ADAS02000016">
        <property type="protein sequence ID" value="OAV96901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GWK5"/>
    <dbReference type="EnsemblFungi" id="PTTG_08937-t43_2">
        <property type="protein sequence ID" value="PTTG_08937-t43_2-p1"/>
        <property type="gene ID" value="PTTG_08937"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08937"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000729">
        <property type="term" value="P:DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17920">
        <property type="entry name" value="DEXHc_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18794">
        <property type="entry name" value="SF2_C_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000296">
        <property type="entry name" value="ATP-dependent DNA helicase RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000340">
        <property type="entry name" value="Bloom syndrome, RecQ helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000495">
        <property type="entry name" value="RecQ family helicase MusN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018982">
        <property type="entry name" value="RQC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF153">
        <property type="entry name" value="RECQ-LIKE DNA HELICASE BLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09382">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00956">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="509"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="714"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="102"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="242"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1022"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1167"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="242"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="269"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="333"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="427"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1051"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1195"/>
            <end position="1210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OAV96901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08937-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="1232" mass="135841" checksum="3888411C6ED0D315" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GWK7</accession>
    <name>A0A180GWK7_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="8">J domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_08936</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000016">
        <property type="protein sequence ID" value="OAV96904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GWK7"/>
    <dbReference type="EnsemblFungi" id="PTTG_08936-t43_1">
        <property type="protein sequence ID" value="PTTG_08936-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08936"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08936"/>
    <dbReference type="OrthoDB" id="5894at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051964">
        <property type="entry name" value="Chaperone_stress_response"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
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        </location>
    </feature>
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        <location>
            <begin position="153"/>
            <end position="185"/>
        </location>
    </feature>
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            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
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            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
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        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="5">
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            <end position="340"/>
        </location>
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            <begin position="341"/>
            <end position="365"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV96904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08936-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="388" mass="45077" checksum="C1A4B2AA0D992DEA" modified="2016-09-07" version="1">MFAKIQQAYEILSDEDERAFYDRHRDELLNANDDGLDDFDPTHLKPTTSRSSLNPGITSKQLLKFFDSTLWKGDLSDSATSFFTIYRNLFNQLSVEEKIARKDTTIVYPSFGTSGSSYDHDIDGTRALKHFYSSWTNFATQKSFEWVEPHRTNQQVDRRIKRLIEKENQRERENARREYNETIRSLVSFVKKRDPRFAASAASNPERWRAQEIQRIKRELREVAERRAKEREDEARQFREQAWQRQKDERKESSGTEDGQAESATEEQSEEEEEEEPEAPVNDWYCAACSKDFKSQGAWDNHERSKKHRQNAQRLRKQMLKEDAELSISSLVSSNLPSNSETRSQTPDALQKVQGVSDQEQTSTAEIEDGLENIALNQPKSSTDNLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GWN6</accession>
    <name>A0A180GWN6_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_02750</name>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000015">
        <property type="protein sequence ID" value="OAV97170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="PTTG_02750-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02750"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02750"/>
    <dbReference type="OrthoDB" id="10067079at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Prkrip1"/>
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        <property type="entry name" value="PRKR-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13507:SF0">
        <property type="entry name" value="PRKR-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06658">
        <property type="entry name" value="DUF1168"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV97170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02750-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="193" mass="21627" checksum="C475ECC3C035AFC5" modified="2016-09-07" version="1">MMKNVQGSSAGAGSGEFHVYKQSRRREYERLRLMDENEEKERLKAEFESKQAEIKQQVEAKTNKNRLKRQKKKDLRKKKSQKLDDQGRPKKANQAGEEQSGDSGSDGSGSETENDPKKKRKKLGAAPIAEGMVFKQPNQNDEDDPENDEQEQAQNPSEDKLDNPDPTSAPSDQLSTTVPVAQSVGLIIVDDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GWP2</accession>
    <name>A0A180GWP2_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">WH1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_26081</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000014">
        <property type="protein sequence ID" value="OAV97215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GWP2"/>
    <dbReference type="EnsemblFungi" id="PTTG_26081-t43_1">
        <property type="protein sequence ID" value="PTTG_26081-t43_1-p1"/>
        <property type="gene ID" value="PTTG_26081"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_26081"/>
    <dbReference type="OrthoDB" id="8963340at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00132">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01205">
        <property type="entry name" value="EVH1_WASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000010">
        <property type="entry name" value="Related to Neural Wiskott-Aldrich syndrome protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033927">
        <property type="entry name" value="WASPfam_EVH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="WH1" evidence="7">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="CRIB" evidence="6">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="WH2" evidence="8">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="215"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="287"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="443"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="489"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OAV97215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_26081-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="51672" checksum="5A3BB83604F041AB" modified="2016-09-07" version="1">MSDLLRLPGPQQVWSYTGLSGALVFGRPSSGIAGSFWFKLIDLSGTRGTIWEHEVYEDGPGRPGHGHFGYHQERTFWHTFQGDDCMIAFVFVNEDEASAIHKKVINRSKYTSKSAAKVKPVEAPLPAAPTPVLAPSGKKKGAIDKSMISAPTDFKRVAHMGYDSNAGFTAENVDPSWAQLLTNLTNMGFSKDEINNSESFIKDYVNQAGGIDQVLSSHNNTSASPPTRVPPPPANPITSKRVKPPAPPAPTRRTGTSNENHNPATPAPSARVPSAPSRAPPIAAAPSRPPPTMPVTPAPSAPVSNPPAPPTAPPPPVSVKSAPPPPSAPPPPVMAPPPPAPPPPPPPSSHAPPPPPPPPPPPPPSSHAPPPPNAPPPPAPPAPPAPAPPAPPPPPSNAPPPPPAPSSSISPVSNGSRADLLSSIQGKGIHALKKTPHGASAHPSDSANPLSQSTRSIGSAEDHDGGGGGGNLAASLAAALNKRKGTMGEDSEEEAESDDEWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GWP4</accession>
    <name>A0A180GWP4_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CxC1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_12763</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000016">
        <property type="protein sequence ID" value="OAV96944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GWP4"/>
    <dbReference type="EnsemblFungi" id="PTTG_12763-t43_2">
        <property type="protein sequence ID" value="PTTG_12763-t43_2-p1"/>
        <property type="gene ID" value="PTTG_12763"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12763"/>
    <dbReference type="OrthoDB" id="2498701at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="138"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV96944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12763-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="469" mass="54052" checksum="80023100658D482C" modified="2016-09-07" version="1">MPTTRFRFKSADRAQRLRRQRNAERDAETLARLRQHELSDRIRQQNSHNQYNPADQPVENAMDYDAPFPAHNDEAVDLADTIGSHEWVDLVEEPLDEIDLAMASERERHRHQARNFNWNALLNQLYPEYMKLKGATQNWAGPNCYNDFHRCTSTYQNKTRRSVDFIGIHGQARQTFTFCECTLDAVKLLQLGYIAASPVQPQTAFSLPLLIFHNHLWNRCHVGALPFMEALNQFLEPRSQRLYAKKGTHARPLRKPFSAAVDLFRQLEEKTDKLIFNVLKMSRQDKLAHNACPACFGPETLNSSEYSDLTRNKLIVCLDGNFQHRHQTKASRNDEQVRIPPTFLRPSAVDEASQEICASETLGMTDDQTDRCTESHKAADDKRNESTWKGCDDTGLMGCCCRHDAVLYFANIYKSGEKRHFPMAIIKKLLADIDPDRPVGFLYDIGCSLDKYIRRVCIHNSLILSLYFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GX17</accession>
    <name>A0A180GX17_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_26119</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000014">
        <property type="protein sequence ID" value="OAV97300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GX17"/>
    <dbReference type="EnsemblFungi" id="PTTG_26119-t43_1">
        <property type="protein sequence ID" value="PTTG_26119-t43_1-p1"/>
        <property type="gene ID" value="PTTG_26119"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_26119"/>
    <dbReference type="OrthoDB" id="2498948at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV97300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_26119-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="277" mass="32564" checksum="BF4742C24BFAA1CC" modified="2016-09-07" version="1">MKMISTHDYHFERCKFYRCCELMFIPSNLSDHLHRLFMHSIELIKASKVSRNYAETTECQNLERRILRDAAEFSDYTRQTIKWLQGSDLYIIQEEWREKEDQLDVLLQVFTDLTHPTSVRQRNNTSALRKHVNELAELTIPLVKLTRIFSKKVSNTATTKLPFTLDTNLNSRTLCTLHELPESIERYFYQLVKAFRDAYTTNELVDRQIVIGYPLRHISHILETTLVLLALYLIPLPTTDTNHDSPESIYKAWFSAWQEAWHGAFNNISCALQTFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GX18</accession>
    <name>A0A180GX18_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MOR2-PAG1_N domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_05795</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000015">
        <property type="protein sequence ID" value="OAV97074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GX18"/>
    <dbReference type="EnsemblFungi" id="PTTG_05795-t43_2">
        <property type="protein sequence ID" value="PTTG_05795-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05795"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05795"/>
    <dbReference type="OrthoDB" id="6287725at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025614">
        <property type="entry name" value="Cell_morpho_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039867">
        <property type="entry name" value="Furry/Tao3/Mor2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295">
        <property type="entry name" value="FURRY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295:SF30">
        <property type="entry name" value="PROTEIN FURRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14222">
        <property type="entry name" value="MOR2-PAG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cell morphogenesis protein N-terminal" evidence="1">
        <location>
            <begin position="38"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV97074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05795-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="211" mass="23342" checksum="BDBD9CDB27C2154B" modified="2016-09-07" version="1" fragment="single">MCWKSCQAKSSDDYAIQRALVTSGNSLNLRRVTAVLSERKHLALVYILCRALISVVQVVTREALGEDLGTRLEEIIFNSIKNADPHVTARTPNKQANMNMFAYLLGALSNIRFTSVSNRFAAEMDAMNWVAHLRDQKDGEARLGYLVQGVQFLKLKVYPLEAFKDTADFIATFATLFESSRGSSVKTAMTKTLGPLLAQVVQSATAEVNHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GXM0</accession>
    <name>A0A180GXM0_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutaminase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_00108</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000016">
        <property type="protein sequence ID" value="OAV97022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GXM0"/>
    <dbReference type="EnsemblFungi" id="PTTG_00108-t43_1">
        <property type="protein sequence ID" value="PTTG_00108-t43_1-p1"/>
        <property type="gene ID" value="PTTG_00108"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00108"/>
    <dbReference type="OrthoDB" id="3918848at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052743">
        <property type="entry name" value="Glutaminase_GtaA"/>
    </dbReference>
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        <property type="entry name" value="GtaA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033433">
        <property type="entry name" value="GtaA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31987:SF1">
        <property type="entry name" value="GLUTAMINASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31987">
        <property type="entry name" value="GLUTAMINASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17168">
        <property type="entry name" value="DUF5127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16335">
        <property type="entry name" value="GtaA_6_Hairpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaminase A N-terminal" evidence="2">
        <location>
            <begin position="149"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaminase A central" evidence="1">
        <location>
            <begin position="391"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV97022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00108-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="720" mass="80730" checksum="2324FA45A6BABFB1" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GXM5</accession>
    <name>A0A180GXM5_PUCT1</name>
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        <submittedName>
            <fullName evidence="8">CBFD_NFYB_HMF domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_01902</name>
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    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000012">
        <property type="protein sequence ID" value="OAV97586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GXM5"/>
    <dbReference type="EnsemblFungi" id="PTTG_01902-t43_2">
        <property type="protein sequence ID" value="PTTG_01902-t43_2-p1"/>
        <property type="gene ID" value="PTTG_01902"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_01902"/>
    <dbReference type="OrthoDB" id="386949at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF9">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00615">
        <property type="entry name" value="CCAATSUBUNTA"/>
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        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
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    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV97586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_01902-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="152" mass="16458" checksum="D56F8B20DD9224FC" modified="2016-09-07" version="1">MEEVERLFPTGSLSVIAPHPTKTPAEADPTPNPTSGGSNNGNTGGAGPIKDTEVDQFNPQHLLLPLSNISKLMKASVPLDSKISNPSKILIQACVSEFISFLSSDANEQVLAEKRRTLNGVDLICAVKRLGFEGYYEALEIYLAKYRTGLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GXP0</accession>
    <name>A0A180GXP0_PUCT1</name>
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        <recommendedName>
            <fullName evidence="4">Chitobiosyldiphosphodolichol beta-mannosyltransferase</fullName>
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    <gene>
        <name evidence="12" type="ORF">PTTG_05886</name>
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    <organism evidence="12">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic surface of the ER.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.142" evidence="3"/>
    <dbReference type="EMBL" id="ADAS02000014">
        <property type="protein sequence ID" value="OAV97304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GXP0"/>
    <dbReference type="EnsemblFungi" id="PTTG_05886-t43_1">
        <property type="protein sequence ID" value="PTTG_05886-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05886"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05886"/>
    <dbReference type="OrthoDB" id="614844at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004578">
        <property type="term" value="F:chitobiosyldiphosphodolichol beta-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026051">
        <property type="entry name" value="ALG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036">
        <property type="entry name" value="BETA1,4 MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036:SF0">
        <property type="entry name" value="CHITOBIOSYLDIPHOSPHODOLICHOL BETA-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OAV97304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05886-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="381" mass="43099" checksum="DF6A6A54D5E65919" modified="2016-09-07" version="1">MVQNPPAIPTLPIVQLSRLMIGSKLIIDWHNTAYSILALKFRTERHPMVRLAKWIEAKFGKHATLHLFVTEAEKQALSEMWNLRGSKKVFYDRPPKSFCRLTVPEIHSFLSQSSFNYNSVLQQMFIGGELEKETILSYEDPETKTAKMKADRPALVVSSTSWTIDEDFSVLIDALSMYARSKKSQKSIPKLLCLITGKGPLKAHYLEIIERTSSEEGWKELGIVCQSVWFDDPEHYRQLLGAADLGISLHQSSSGLDLPMKVVDMFGCRLPVCARNFNCISELVKHGQNGLVFDSAVELSEQLKELLTGFNPARSMKELASSNSNSRTLKDLQTGIENAMYGAVLSTNDKDLDRLQSSGCRSWSCWEEEWNQQVFQTVQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GXP5</accession>
    <name>A0A180GXP5_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_01910</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000012">
        <property type="protein sequence ID" value="OAV97597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GXP5"/>
    <dbReference type="EnsemblFungi" id="PTTG_01910-t43_2">
        <property type="protein sequence ID" value="PTTG_01910-t43_2-p1"/>
        <property type="gene ID" value="PTTG_01910"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_01910"/>
    <dbReference type="OrthoDB" id="2500038at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV97597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_01910-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="17887" checksum="0CB9A1F0410452F2" modified="2016-09-07" version="1">MCVEPTREDRAAKHRQNNFMLLNIEGTTSLLAQVVTPKTFLAALITLDGRVVAFHIHPSAIVADEDEQSDGSDQAKIAAAIASGLWREDCYQRPFSSTSKTEVAREVRNLDAVCELGQLRLNFTPPFLLVLVGKTGSVSIDTLSMKAQLLQDQLKGPFSNIVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GXQ8</accession>
    <name>A0A180GXQ8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CHY-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_05761</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000015">
        <property type="protein sequence ID" value="OAV97128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GXQ8"/>
    <dbReference type="EnsemblFungi" id="PTTG_05761-t43_2">
        <property type="protein sequence ID" value="PTTG_05761-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05761"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05761"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045041">
        <property type="term" value="P:protein import into mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052604">
        <property type="entry name" value="Mito_Tim_assembly_helper"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28082:SF2">
        <property type="entry name" value="CHY-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28082">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV97128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05761-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="87" mass="10087" checksum="9FBC030662236AB6" modified="2016-09-07" version="1">MVMMCKKCKKAFRKMVEEFEESDEYCPHCDNHYIIEAKTAKPMVGIEGEDARVDARGLATRDERSNKVDRQAELNKLLEEEFASKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GXR1</accession>
    <name>A0A180GXR1_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BRCA2 OB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_01925</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000012">
        <property type="protein sequence ID" value="OAV97617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GXR1"/>
    <dbReference type="EnsemblFungi" id="PTTG_01925-t43_1">
        <property type="protein sequence ID" value="PTTG_01925-t43_1-p1"/>
        <property type="gene ID" value="PTTG_01925"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_01925"/>
    <dbReference type="OrthoDB" id="21095at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015525">
        <property type="entry name" value="BRCA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015252">
        <property type="entry name" value="BRCA2_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036315">
        <property type="entry name" value="BRCA2_hlx_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015187">
        <property type="entry name" value="BRCA2_OB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289:SF0">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN BRCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09169">
        <property type="entry name" value="BRCA-2_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09103">
        <property type="entry name" value="BRCA-2_OB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81872">
        <property type="entry name" value="BRCA2 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Breast cancer type 2 susceptibility protein helical" evidence="3">
        <location>
            <begin position="805"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="domain" description="BRCA2 OB1" evidence="2">
        <location>
            <begin position="858"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="946"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1054"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="363"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="434"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1058"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1073"/>
            <end position="1087"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV97617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_01925-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1132" mass="122596" checksum="7719FE4873B407DF" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GY30</accession>
    <name>A0A180GY30_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pyruvate carboxylase</fullName>
            <ecNumber evidence="6">6.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PTTG_08105</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="16">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20844"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="6.4.1.1" evidence="6"/>
    <dbReference type="EMBL" id="ADAS02000016">
        <property type="protein sequence ID" value="OAV96913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GY30"/>
    <dbReference type="EnsemblFungi" id="PTTG_08105-t43_1">
        <property type="protein sequence ID" value="PTTG_08105-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08105"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08105"/>
    <dbReference type="OrthoDB" id="196847at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07937">
        <property type="entry name" value="DRE_TIM_PC_TC_5S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000018">
        <property type="entry name" value="Biotin carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000033">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000012">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.600.10">
        <property type="entry name" value="pyruvate carboxylase f1077a mutant domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055268">
        <property type="entry name" value="PCB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005930">
        <property type="entry name" value="Pyruv_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009554">
        <property type="entry name" value="PRK12999.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01235">
        <property type="entry name" value="pyruv_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778">
        <property type="entry name" value="PYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778:SF2">
        <property type="entry name" value="PYRUVATE CARBOXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00878">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
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    </dbReference>
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        <property type="entry name" value="post-HMGL domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0092">Biotin</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="12">
        <location>
            <begin position="55"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="11">
        <location>
            <begin position="175"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="13">
        <location>
            <begin position="593"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="602"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="674"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="9">
        <location>
            <position position="771"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="801"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="803"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="936"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OAV96913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08105-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="1127" mass="123574" checksum="A6B98A4363BDE046" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GYM2</accession>
    <name>A0A180GYM2_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_00147</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PpiC/parvulin rotamase family. PIN4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="6"/>
    <dbReference type="EMBL" id="ADAS02000013">
        <property type="protein sequence ID" value="OAV97418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GYM2"/>
    <dbReference type="EnsemblFungi" id="PTTG_00147-t43_1">
        <property type="protein sequence ID" value="PTTG_00147-t43_1-p1"/>
        <property type="gene ID" value="PTTG_00147"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00147"/>
    <dbReference type="OrthoDB" id="1911748at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043323">
        <property type="entry name" value="PIN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45995">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13616">
        <property type="entry name" value="Rotamase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PpiC" evidence="8">
        <location>
            <begin position="29"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
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                <person name="Tanner A.C."/>
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                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12">1-1 BBBD Race 1</strain>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12">1-1 BBBD Race 1</strain>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
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        <property type="gene ID" value="PTTG_12012"/>
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        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SUV39H"/>
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    </evidence>
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            <dbReference type="Google" id="ProtNLM"/>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
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                <person name="Feldgarden M."/>
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                <person name="Zeng Q."/>
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                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
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                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
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                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
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                <person name="Park D."/>
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                <person name="Saif S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">1-1 BBBD Race 1</strain>
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    </reference>
    <reference evidence="7" key="2">
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
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                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
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    <reference evidence="8" key="4">
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
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        <property type="gene ID" value="PTTG_11806"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_11806"/>
    <dbReference type="OrthoDB" id="39175at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF33">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
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            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="427"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="EMBL" id="OAV98095.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11806-t43_2-p1"/>
        </source>
    </evidence>
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        <source>
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        </source>
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</entry>
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    <name>A0A180GZR2_PUCT1</name>
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    <organism evidence="1">
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        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
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    <reference evidence="1" key="1">
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000011">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblFungi" id="PTTG_09808-t43_2">
        <property type="protein sequence ID" value="PTTG_09808-t43_2-p1"/>
        <property type="gene ID" value="PTTG_09808"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09808"/>
    <dbReference type="OrthoDB" id="775571at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <source>
            <dbReference type="EMBL" id="OAV97742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09808-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="297" mass="33093" checksum="A40C2C771710CB2F" modified="2016-09-07" version="1">MKLQISAWRHGIKLSDDPLATIQRSKGVSLPTMQDIEQELDIGDFLKAISTGLKPYDNQVGGSRHIDQRRKGLIETVEERLRSCRVDRAGLLQPMELPSLDLLTEVTVIESLQSRSLEIDQCLLSFFLIDLDFLDHLKILGQFMFCHDVMFNQRLTNALFSSSPGEDSSQRTGWRTVGVSGKLLRRGIWPPGGFDLAFALRTVILDSVAPDQRPAGYRVTLEDQLSFAIRPGIKDSAAGESGYRANTIDGFGFLMIDFKPPCLLPSHCLSHLQACTTWLAENSGPGVTFFTRHCKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GZS2</accession>
    <name>A0A180GZS2_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_11695</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000008">
        <property type="protein sequence ID" value="OAV98346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblFungi" id="PTTG_11695-t43_1">
        <property type="protein sequence ID" value="PTTG_11695-t43_1-p1"/>
        <property type="gene ID" value="PTTG_11695"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_11695"/>
    <dbReference type="OrthoDB" id="2500414at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="290"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV98346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11695-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="391" mass="43205" checksum="A04ED9D48FD5B8A5" modified="2016-09-07" version="1">MGLVVHPMFCLRSQVPANHPDPLVLTGKKVAWRAWSYLDSFVKTSGPSSILRCLPGYNTSKPVDMDVIPKESFKNQGCEPIVRRLVDASNIWEILLSLDESAKQTISPGGWDLLNLLVTAWEIDFKNQEVPEIQTQSLHQLSKKGPIQERKTCFPHFLRQLGDAPAGGFRIDTRVYDIIFEPFSRNSASPTYVWGCATNSLARAQELSIRLLHLLDRLDCIQLLEPGRLREDIVIRMLGLETNALRTFVNVLPEDHLLRTRLLSRFLESVTRSTPTPLVGFTLSNSQSSAASLSPGQAGLNGTASTTGLRRTPSRTALKFDSQSSISNSKSQLLSLSSLITQLLPRPLLEIPATGKRRSSAGSKLDPSALFKCRIALSLRLECHLGVVVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H3B6</accession>
    <name>A0A180H3B6_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AMP-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_06670</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000003">
        <property type="protein sequence ID" value="OAV99485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H3B6"/>
    <dbReference type="EnsemblFungi" id="PTTG_06670-t43_2">
        <property type="protein sequence ID" value="PTTG_06670-t43_2-p1"/>
        <property type="gene ID" value="PTTG_06670"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06670"/>
    <dbReference type="OrthoDB" id="1898221at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF149">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="51"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV99485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06670-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="455" mass="50254" checksum="8B4516611AE5AB6F" modified="2016-09-07" version="1">MEATGNQIIYQSPWGSILPQPAKPLNIWDFIRSTISEEALKSHESRCIEVESGEAWTLSQILNHAETLAKYLVDSFGGRRSTWNTEGANVDYDVITIGVLAPNIPAFPIVSYSCMAAGLPVAPLPVGSSSSEIAYLVRLAQCEIVFVHPSQLSIIKKSGYPPERIILTDSVEGWDGQTLPDLMVIAEKLPAFATDRKHPMPKHEVALVVFSSGSSGNPKAVMITHQNICALLISRALTSGPNPGVKKKTVLLSVLPMYHIYGFLLSVIIPLLDGVTQCTLRSWNVQTVFRAIAKYNITTMGLVPTMALQIISAADRLENVDLSSLTSVFVGSSAINPSQKQRLFDLLKSRGALAAEHDGSSIIDGYGMNYLGYHHLVKGRHSCHPCTSGNRRFFDAWLPSAYHFRILQQSYGRRYAQAHGWGVVFAWPYNHERLSCRSGGFSRHLHIGWVVENWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H3C3</accession>
    <name>A0A180H3C3_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">DNA excision repair protein ERCC-5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PTTG_02358</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="19">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Single-stranded DNA endonuclease involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Essential for the incision step of excision-repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the XPG/RAD2 endonuclease family. GEN subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000004">
        <property type="protein sequence ID" value="OAV99109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_02358-t43_1">
        <property type="protein sequence ID" value="PTTG_02358-t43_1-p1"/>
        <property type="gene ID" value="PTTG_02358"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02358"/>
    <dbReference type="OrthoDB" id="31113at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048256">
        <property type="term" value="F:flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09904">
        <property type="entry name" value="H3TH_XPG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09868">
        <property type="entry name" value="PIN_XPG_RAD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000025">
        <property type="entry name" value="DNA repair protein RAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000030">
        <property type="entry name" value="Flap endonuclease GEN-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001044">
        <property type="entry name" value="XPG/Rad2_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-G CELLS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171:SF7">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00066">
        <property type="entry name" value="XRODRMPGMNTG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="16">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="15">
        <location>
            <begin position="854"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="131"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="310"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="529"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="563"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1125"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="456"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="591"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="603"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="640"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="673"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="690"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="702"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="716"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1144"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1189"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1220"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1254"/>
            <end position="1266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OAV99109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02358-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1285" mass="142848" checksum="C6B8931639A38931" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H4C3</accession>
    <name>A0A180H4C3_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="7">4.2.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ATP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_25245</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6S)-NADHX + ATP = ADP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.93" evidence="7"/>
    <dbReference type="EMBL" id="ADAS02000002">
        <property type="protein sequence ID" value="OAV99661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H4C3"/>
    <dbReference type="EnsemblFungi" id="PTTG_25245-t43_2">
        <property type="protein sequence ID" value="PTTG_25245-t43_2-p1"/>
        <property type="gene ID" value="PTTG_25245"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_25245"/>
    <dbReference type="OrthoDB" id="8110916at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047453">
        <property type="term" value="F:ATP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF C-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="100"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="141"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OAV99661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_25245-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="254" mass="28169" checksum="D44F00FB77A2244E" modified="2016-09-07" version="1">MVIKTYSPDLMVHSYLSSPEHPEAYAAHQKEFDQLMGRLHVLVVGPGLGRDTEMQDWAEWTLRTAMKKKIHLVLDADALWLLQNKPEILRGYPHAILTPNHVEFQRLLKACSIDPRENDGDDGRLALELSKALGGCTILQKGAMDLVARVGSEVAKVSCQGSPKRCGGQGDILSGLVGTWCAWSKLYLDTKPKSHDQPISPEEAWVIAAVLGAEITRTCSRLAYQKFGRSMQSSDMLSYIGEAFEQVMHGHTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H5F0</accession>
    <name>A0A180H5F0_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RanBD1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_07361</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000001">
        <property type="protein sequence ID" value="OAW00032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H5F0"/>
    <dbReference type="EnsemblFungi" id="PTTG_07361-t43_1">
        <property type="protein sequence ID" value="PTTG_07361-t43_1-p1"/>
        <property type="gene ID" value="PTTG_07361"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_07361"/>
    <dbReference type="OrthoDB" id="185618at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000769">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF87">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE RANBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH domain-like"/>
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    </dbReference>
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        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
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        </location>
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        </location>
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            <begin position="481"/>
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        </location>
    </feature>
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        <location>
            <begin position="11"/>
            <end position="22"/>
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        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="490"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAW00032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_07361-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="507" mass="54487" checksum="A44E90204B066419" modified="2016-09-07" version="1">MADKTSPTTAEAHEVKTVREQVEGMGVETGQQTAVSSSDQKNLKHSTPRGQHNELDNSPPSSTKAKELPASWDAYADLQSPFACFAPRTTTWSYAQPSDQGPGSPKEGSNLHARASAPPPKQVSTAPAFSLTGQQQASANQLCPNKFPSGSDFFLKGKRNTSVADPSPVLDEDSASQSKKPKLPPPFNSHPDLQPPSAQPSTVSNFLNVHPPSSKASSACADNGETDEPPSSTRSPANPSPQPQQPSSQSLGFSAFATSAGFGDSSKKPTVDWSDGRGSSPSVFDQQPPSSSKPADPSQPDSQQPPVSEWGGGQTAERKTHLALSAELGQEDKHEGFLATEVITGEENEELVKSVRCKMFTLGEDQSWRERGTGGLRLLRTKEEPYRYRLIMRADAVLRVLLNVPIFNGFSYRPTQDKFLTFASTVALNPSTGSGPGSTAPAELEGESGKTQFQQYLCRFGKPEARQEIIDSIEQCLKELARSNPDQSTQDDKENRENASVDASDLV</sequence>
</entry>
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    <accession>A0A0C4EQ10</accession>
    <name>A0A0C4EQ10_PUCT1</name>
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        <submittedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_02863</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000043">
        <property type="protein sequence ID" value="OAV94057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4EQ10"/>
    <dbReference type="EnsemblFungi" id="PTTG_02863-t43_2">
        <property type="protein sequence ID" value="PTTG_02863-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02863"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02863"/>
    <dbReference type="OMA" id="RAKEECQ"/>
    <dbReference type="OrthoDB" id="6159137at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="34"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="184"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV94057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02863-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="215" mass="25364" checksum="D119A434E6713BCC" modified="2015-04-01" version="1">MERSRSPLGDRIGPANGSQTTDRGRSSDVANPGNNIHVSGISLKADDPDLEDLFSKYGTVTKSQLMRDPHTREIRGFGFVTMETSEQADIAISSLHGTEFLGKTLTVEKARRARGRTPTPGQYRGPAKRDDPHRSYEPRGGYPYRYDPYDRRYDDRLRRDYDDGRYPIRRDYEDRRYRYDDRVPSYDRYGYAGPMRDHDRFDRRRDDRDYDRRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4F0P4</accession>
    <name>A0A0C4F0P4_PUCT1</name>
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        <submittedName>
            <fullName evidence="11">AcidPPc domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PTTG_06661</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
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        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the type 2 lipid phosphate phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000003">
        <property type="protein sequence ID" value="OAV99472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="ADAS02000003">
        <property type="protein sequence ID" value="OAV99473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4F0P4"/>
    <dbReference type="EnsemblFungi" id="PTTG_06661-t43_1">
        <property type="protein sequence ID" value="PTTG_06661-t43_1-p1"/>
        <property type="gene ID" value="PTTG_06661"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_06661-t43_3">
        <property type="protein sequence ID" value="PTTG_06661-t43_3-p1"/>
        <property type="gene ID" value="PTTG_06661"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06661"/>
    <dbReference type="OMA" id="ADDCPCY"/>
    <dbReference type="OrthoDB" id="301434at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF28">
        <property type="entry name" value="DIHYDROSPHINGOSINE 1-PHOSPHATE PHOSPHATASE LCB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="401"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="9">
        <location>
            <begin position="47"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV99472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06661-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="426" mass="47578" checksum="78DA80E48ECF259B" modified="2015-04-01" version="1">MTILESIQRSYRSGPLDRLMVHSSWLGSHQFFILALPLIFWLGDHHFGRSQVYILGMSVYFTGILKDLFCIPRPYSPPIQRLAISNHASEYGFPSSHSAISASTFLMGLQYALHVQSIVQQLALFLGLILYGFLLVFGRVYCGMHSIQDVACGWTIGVFAWTIHRVFGEVVERWATSTQASVPLVLIVCGLILTAAHPQPVDDCPCFEDSTAFVAVSVGVMIGHRIANDLILHQMKDPALSGLRFPQALIKLFLGLAVIFGWRFLMKELLSRLLPPLFRLFAPLLELPRKHYAPTKDYGRYRDELRERGRRTGLSARLIPSVVDLPNMTRTLRNRFAPESPPADSPVAHPAQALHPALHNRTPNAIEIDPLVDAQKPSTLDSKLPPAPPSKHYDVDVLMRLIVYVGIGMLSSGLLPIFFDRIGLDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4F3U2</accession>
    <name>A0A0C4F3U2_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_12518</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000136">
        <property type="protein sequence ID" value="OAV89217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4F3U2"/>
    <dbReference type="EnsemblFungi" id="PTTG_12518-t43_2">
        <property type="protein sequence ID" value="PTTG_12518-t43_2-p1"/>
        <property type="gene ID" value="PTTG_12518"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12518"/>
    <dbReference type="OMA" id="DWASIPR"/>
    <dbReference type="OrthoDB" id="271862at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22453">
        <property type="entry name" value="KH-I_MUG60_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056553">
        <property type="entry name" value="KH_Mug60-KHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627:SF76">
        <property type="entry name" value="KH DOMAIN-CONTAINING PROTEIN YLL032C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627">
        <property type="entry name" value="SCP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24563">
        <property type="entry name" value="KH_Mug60-KHD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="246"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="331"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV89217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12518-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="636" mass="71492" checksum="D28DB20D4581CFDD" modified="2015-04-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4F5X2</accession>
    <name>A0A0C4F5X2_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_08527</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
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                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
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                <person name="Stolte C."/>
                <person name="Sykes S."/>
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                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
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                <person name="Nusbaum C."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
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                <person name="Levin J.Z."/>
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            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
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                <person name="Feldgarden M."/>
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                <person name="Zeng Q."/>
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                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
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                <person name="Chapman S.B."/>
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                <person name="Heiman D."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Khalil H.B."/>
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                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
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                <person name="Szabo L.J."/>
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                <person name="Zeng Q."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">1-1 BBBD Race 1</strain>
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        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02001560">
        <property type="protein sequence ID" value="OAV86157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G0H5"/>
    <dbReference type="EnsemblFungi" id="PTTG_30052-t43_1">
        <property type="protein sequence ID" value="PTTG_30052-t43_1-p1"/>
        <property type="gene ID" value="PTTG_30052"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_30052"/>
    <dbReference type="OrthoDB" id="2506732at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV86157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_30052-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="486" mass="55902" checksum="3B66337F11B0A2BA" modified="2016-09-07" version="1">MMFGTSVFHSYVHEWSCQVKYNPRLNKAWGLSDGEGLERLWAFLSPLVSILRVSSCFRRLMAVHNRTLYYSAGLNETAGEWLLSKFASADDIIKTARIALDALNQLSNPHSMTAGTYTDNFFHQQWEDKRAYHLETTRSYQEKQKKELGQLLCLEEQLETEWSCEGLSPAQGIARARIVSCLSDQILEQRTKVGDPTMLENLSVPEHDKMLKIWYLKTDLRHKFLALIEEKKPLVCVCRPGEQTTLGTDGQQKLIKSVRKQAQALRKVLNKYNSQVREFIRLFPERAHPRQLEYNELLKLESEDAFWNDGLFTNRNEPWAIDPITQKGIQHLAAYNRGLEEQRCLGWEVRRAMRWAVERHDTLSHLILQMTTVTGGSNCEWTELAAHPILQTLVAHPILQSLAAPKKTTVARSLLQYELVKIHQLQIAWQRTCITILNNTQQQLGDKNLRELWSRQLEHIELGYYSMIPGSDTDAGETVNGASKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G2G9</accession>
    <name>A0A180G2G9_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ADP-ribosylation factor-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PTTG_09161</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000820">
        <property type="protein sequence ID" value="OAV86778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G2G9"/>
    <dbReference type="EnsemblFungi" id="PTTG_09161-t43_1">
        <property type="protein sequence ID" value="PTTG_09161-t43_1-p1"/>
        <property type="gene ID" value="PTTG_09161"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09161"/>
    <dbReference type="OrthoDB" id="414781at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="88"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="159"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="262"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OAV86778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09161-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="35944" checksum="EAD8B34CA8C9E199" modified="2016-09-07" version="1">MLEKLSVVTMLEKVKTIFSPSQPGLNPSQIAPTIGQNIGRITLSSTYLKFWDLGGSKDIRSIWEKYYEEADAICWVLDSQDRFRNGWKSDGNARNSHEAQGGSGGDSKGKGKAKAVNESPAGSERGEGWAELEKVLQHPSIAHSNIPVLVIANKQDREARPLPGQKSAAYDPPLQSSTSTSEVDLEDQHHQQLDPMTVEEIKEMFNRLVMESERSEKARSLGLSEAHVLGVSSLTGQGIREAINWLFFRVATKGQSRKTQHLKNQQNLLKQQQQEQQRSNPYLQLSTNEHSTVNHNRDPSSLKNNFIKLVDLSKFNHSTSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G3N0</accession>
    <name>A0A180G3N0_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-rRNA-processing protein IPI3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PTTG_05581</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the RIX1 complex, composed of IPI1, RIX1/IPI2 and IPI3 in a 1:2:2 stoichiometry. The complex interacts (via RIX1) with MDN1 (via its hexameric AAA ATPase ring) and the pre-60S ribosome particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the WD repeat IPI3/WDR18 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000514">
        <property type="protein sequence ID" value="OAV87244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G3N0"/>
    <dbReference type="EnsemblFungi" id="PTTG_05581-t43_1">
        <property type="protein sequence ID" value="PTTG_05581-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05581"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05581"/>
    <dbReference type="OrthoDB" id="756370at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120330">
        <property type="term" value="C:rixosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045227">
        <property type="entry name" value="WDR18/Ipi3/RID3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18763:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18763">
        <property type="entry name" value="WD-REPEAT PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="304"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OAV87244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05581-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="397" mass="42419" checksum="CBCDF2679C19FF38" modified="2016-09-07" version="1">MCSVWSIPTLVDEALDSNLSHTPYSIFSDHTLPISDLCISRGCFPDLRIFTASLDQTIKIWSPLYPSSPLLSTFAVPGPAHHLAIDPLERFIIVSYATMLKNEAEESANDTTAAQPQQSTAIPASTVRIIPLFSRPSHSATQGGVSRVHGPGGLVQQIESGDQEVTYTSPRSTQITALHLPSPIVSSSVVLCGLSNGKIVHLSMPSLQPLGQTVPHPNATGDVKDPVVYMNTFARPVDLLSSSAADALSGPDLAGSSVISPRPVGVLGRVVGRNGDPQRGPGSLQASHTQQACREMYTMLKIEQASDPGLVEDDPFSLPSRETRPDNSALHALPDHSHQSIVQDHQQNSQTDSIEKLTAETSHLKAQLKQALAFNDSLWAGIVDGTLTFKEGTLPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G4K8</accession>
    <name>A0A180G4K8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_11777</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000815">
        <property type="protein sequence ID" value="OAV86783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G4K8"/>
    <dbReference type="EnsemblFungi" id="PTTG_11777-t43_2">
        <property type="protein sequence ID" value="PTTG_11777-t43_2-p1"/>
        <property type="gene ID" value="PTTG_11777"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_11777"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV86783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11777-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="282" mass="30238" checksum="BAB26202CDED62CA" modified="2016-09-07" version="1" fragment="single">MTIIFPHFQFKNRTLFLRMNTRHTVSSRNFLFLAFFFLIVSNSVFTFRTKNSQTNTRLQLRSMVPETRMPIAANRRLTKRTFGPKPDTPGPKPDSPLKDGLPPKGSLPSGDLDLGLSGDKDKGPPPKSGSPGKDSGKPLDLGAPLPKPGPPKSGPPSKLGPLPKPGPLLGSESSEISGNVETKTIRIGGGKDCDKKKKINKNPPEKSTDDSQLEISGPTVIKIEVVSGGCSTCCKTCTEDKLKEATDSPPPKKKTPDEPIPEISSGKGGEPNKPKPIPEISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G511</accession>
    <name>A0A180G511_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Vacuolar calcium ion transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PTTG_03007</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="15">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Has a role in promoting intracellular calcium ion sequestration via the exchange of calcium ions for hydrogen ions across the vacuolar membrane. Involved also in manganese ion homeostasis via its uptake into the vacuole.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Vacuole membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000313">
        <property type="protein sequence ID" value="OAV87690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G511"/>
    <dbReference type="EnsemblFungi" id="PTTG_03007-t43_1">
        <property type="protein sequence ID" value="PTTG_03007-t43_1-p1"/>
        <property type="gene ID" value="PTTG_03007"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03007"/>
    <dbReference type="OrthoDB" id="1699231at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004798">
        <property type="entry name" value="CAX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00378">
        <property type="entry name" value="cax"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF22">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0050">Antiport</keyword>
    <keyword evidence="6 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 10" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008109570" description="Vacuolar calcium ion transporter" evidence="11">
        <location>
            <begin position="20"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="12">
        <location>
            <begin position="4"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="12">
        <location>
            <begin position="218"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OAV87690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03007-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="362" mass="38307" checksum="D7E877D77038D2D8" modified="2016-09-07" version="1">MADFIISFVAIIPLAGLLGDATEQVALKVGSTLGGLLNATFGNAVEAIVGIVALSQNQLRIVQTSMLGSILSNILLVLGCSFIAAGFKFKESRFQATAAQASASLMLLACIALVIPAAYFSSFLDKINSGRAQHPGAIPSAQDLANGLALGLSSTHDLPGKEVAQHGLLVISRGTSVLMLAMYILYLYFQLSTHSYLYEDTSSETEEEEARMNFRTATGALVGVTVVTAFCAEYLVDSIDEFAQQAGIPKAFIGLILLPIVGNAAEHVTAVFMAMKGKMELAIGVAVGSSIQIAVGVIPIMVIIGWATGKELTLFFENFETICLVFSVLLTTLLIQDGKSNYMEGAMLVSLYVIIGLAFWVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G600</accession>
    <name>A0A180G600_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Prefoldin subunit 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_04747</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000266">
        <property type="protein sequence ID" value="OAV87852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G600"/>
    <dbReference type="EnsemblFungi" id="PTTG_04747-t43_2">
        <property type="protein sequence ID" value="PTTG_04747-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04747"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04747"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23156">
        <property type="entry name" value="Prefoldin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000014">
        <property type="entry name" value="Prefoldin subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016655">
        <property type="entry name" value="PFD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV87852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04747-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="140" mass="16288" checksum="01B4FBB6FDA3C003" modified="2016-09-07" version="1">MEKSTLQKQLGFEEKIPELERTLEAVELLQLKKEANESLETHFELADTVYTSAVVEPVEEVYLWLGANTMLAYPLSEARELLKNKIESAKLKLEEVSEEQAYLRNQITTTQVNIARVFNWDVKRRKERRAKEAENNHKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G611</accession>
    <name>A0A180G611_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PTTG_04398</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="19 20" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="19">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="5"/>
    <dbReference type="EMBL" id="ADAS02000220">
        <property type="protein sequence ID" value="OAV88048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G611"/>
    <dbReference type="EnsemblFungi" id="PTTG_04398-t43_1">
        <property type="protein sequence ID" value="PTTG_04398-t43_1-p1"/>
        <property type="gene ID" value="PTTG_04398"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04398"/>
    <dbReference type="OrthoDB" id="413077at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1150:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11610:FF:000009">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000003">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="17">
        <location>
            <begin position="546"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OAV88048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04398-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="949" mass="106559" checksum="AB82F708D97DC1A3" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G748</accession>
    <name>A0A180G748_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-alpha-acetyltransferase 40</fullName>
            <ecNumber evidence="4">2.3.1.257</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PTTG_12323</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="16">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-seryl-[histone H2A] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[histone H2A] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50600"/>
            <dbReference type="Rhea" id="RHEA-COMP:12742"/>
            <dbReference type="Rhea" id="RHEA-COMP:12744"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.257"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-terminal L-seryl-[histone H4] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[histone H4] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50596"/>
            <dbReference type="Rhea" id="RHEA-COMP:12740"/>
            <dbReference type="Rhea" id="RHEA-COMP:12743"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.257"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. NAA40 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.257" evidence="4"/>
    <dbReference type="EMBL" id="ADAS02000174">
        <property type="protein sequence ID" value="OAV88471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G748"/>
    <dbReference type="EnsemblFungi" id="PTTG_12323-t43_3">
        <property type="protein sequence ID" value="PTTG_12323-t43_3-p1"/>
        <property type="gene ID" value="PTTG_12323"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12323"/>
    <dbReference type="OrthoDB" id="424551at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043998">
        <property type="term" value="F:histone H2A acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010485">
        <property type="term" value="F:histone H4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039949">
        <property type="entry name" value="NAA40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20531">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20531:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="13">
        <location>
            <begin position="90"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OAV88471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12323-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="203" mass="23293" checksum="C009E05FA596FE2C" modified="2016-09-07" version="1">MSEWTAQTCQDGHEPRASAKSSSRSPTTQVINLTQNKLSGDIIELDIMLDPIRRSADLTPELKKECFEIFESNMKQIYLKSSHGYKPKAKKRELFHPDSRFLLASTPDEGKSLLPVHGFLMWRFDFEECFSPEEGMVEVAYCYEIQLKPETRGKGLGKLLMEILERIGSSWAMKKLMLTVQTGTWIFLSPSPDDSMRYPQTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G833</accession>
    <name>A0A180G833_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-alpha-acetyltransferase 40</fullName>
            <ecNumber evidence="4">2.3.1.257</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PTTG_12323</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="14">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-seryl-[histone H2A] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[histone H2A] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50600"/>
            <dbReference type="Rhea" id="RHEA-COMP:12742"/>
            <dbReference type="Rhea" id="RHEA-COMP:12744"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.257"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-terminal L-seryl-[histone H4] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[histone H4] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50596"/>
            <dbReference type="Rhea" id="RHEA-COMP:12740"/>
            <dbReference type="Rhea" id="RHEA-COMP:12743"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.257"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. NAA40 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.257" evidence="4"/>
    <dbReference type="EMBL" id="ADAS02000174">
        <property type="protein sequence ID" value="OAV88472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G833"/>
    <dbReference type="EnsemblFungi" id="PTTG_12323-t43_2">
        <property type="protein sequence ID" value="PTTG_12323-t43_2-p1"/>
        <property type="gene ID" value="PTTG_12323"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12323"/>
    <dbReference type="OrthoDB" id="424551at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043998">
        <property type="term" value="F:histone H2A acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010485">
        <property type="term" value="F:histone H4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039949">
        <property type="entry name" value="NAA40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20531">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20531:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OAV88472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12323-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="151" mass="17497" checksum="5EB455BCF4785439" modified="2016-09-07" version="1">MKQIYLKSSHGYKPKAKKRELFHPDSRFLLASTPDEGKSLLPVHGFLMWRFDFEECFSPEEGMVEVAYCYEIQLKPETRGKGLGKLLMEILERIGSSWAMKKLMLTVQTENENAVQFYRSLNFLPDEISPSQVEQPEGEQKADYEILSKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G8T6</accession>
    <name>A0A180G8T6_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_28807</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
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                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
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                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
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                <person name="Zeng Q."/>
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                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000183">
        <property type="protein sequence ID" value="OAV88353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G8W9"/>
    <dbReference type="EnsemblFungi" id="PTTG_08444-t43_2">
        <property type="protein sequence ID" value="PTTG_08444-t43_2-p1"/>
        <property type="gene ID" value="PTTG_08444"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08444"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053238">
        <property type="entry name" value="RING-H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155:SF627">
        <property type="entry name" value="OS06G0192800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008109693" evidence="6">
        <location>
            <begin position="24"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="558"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="174"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="431"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="489"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="200"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="507"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OAV88353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08444-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="591" mass="64273" checksum="4436B3C373DEFF74" modified="2016-09-07" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G8X4</accession>
    <name>A0A180G8X4_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methyltransf_11 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_28802</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000139">
        <property type="protein sequence ID" value="OAV89137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G8X4"/>
    <dbReference type="EnsemblFungi" id="PTTG_28802-t43_1">
        <property type="protein sequence ID" value="PTTG_28802-t43_1-p1"/>
        <property type="gene ID" value="PTTG_28802"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_28802"/>
    <dbReference type="OrthoDB" id="411785at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051419">
        <property type="entry name" value="Lys/N-term_MeTrsfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176:SF80">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="2"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OAV89137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_28802-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="156" mass="18030" checksum="39B84C3658CA2649" modified="2016-09-07" version="1">MLGCGNSSLSKDMYDSGFQKIVNVDYSQILIDRMRSQHDENCPEMSWIQADVRHLPFADSSFDAAIDKGTMDALMCAKGDVWDPPEEVVENCKMEVDEVARILKPGGVFIYVTFGQPHFRKRHLQRPGIWSVEVIELGDMFHYYFYVMRKQMEPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G9W8</accession>
    <name>A0A180G9W8_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Threonine dehydratase</fullName>
            <ecNumber evidence="12">4.3.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Threonine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PTTG_06119</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="16">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-threonine = 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22108"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.3.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="12"/>
    <dbReference type="EMBL" id="ADAS02000129">
        <property type="protein sequence ID" value="OAV89410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G9W8"/>
    <dbReference type="EnsemblFungi" id="PTTG_06119-t43_1">
        <property type="protein sequence ID" value="PTTG_06119-t43_1-p1"/>
        <property type="gene ID" value="PTTG_06119"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06119"/>
    <dbReference type="OrthoDB" id="4418812at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00054"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04907">
        <property type="entry name" value="ACT_ThrD-I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1020.10:FF:000001">
        <property type="entry name" value="L-threonine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000008">
        <property type="entry name" value="L-threonine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1020.10">
        <property type="entry name" value="Biosynthetic Threonine Deaminase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001721">
        <property type="entry name" value="TD_ACT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038110">
        <property type="entry name" value="TD_ACT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005787">
        <property type="entry name" value="Thr_deHydtase_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01124">
        <property type="entry name" value="ilvA_2Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006674">
        <property type="entry name" value="PRK09224.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF11">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00585">
        <property type="entry name" value="Thr_dehydrat_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51672">
        <property type="entry name" value="ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ACT-like" evidence="14">
        <location>
            <begin position="482"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001274"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004810"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362012"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OAV89410.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06119-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="570" mass="61988" checksum="91157E81A1924F06" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GAG0</accession>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_08581</name>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000121">
        <property type="protein sequence ID" value="OAV89696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GAG0"/>
    <dbReference type="EnsemblFungi" id="PTTG_08581-t43_1">
        <property type="protein sequence ID" value="PTTG_08581-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08581"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08581"/>
    <dbReference type="OrthoDB" id="10486286at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV89696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08581-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="503" mass="58455" checksum="D8768420514EC004" modified="2016-09-07" version="1">MYSLREARSRERKESDHESLQFTMDPSTDESQQRPIEADINEYKRFVTLHSCKENQQTEHFAVVSKVVGIERLRNYHDDLEEILKLKSPDTNQEDVISQWVKFFQGYKSSFTRLKNVLNLTDADHENQIKQLVELAHFIRFPTVKEPSGMSQEEMLTKIQSTKESRKSQEELTEFKRFKKLHVYPEEADKKFISIVEAAGVARLRKYHEDLEMCIESLEVGCQSSFRVYFKRFYQGASDSLRELKENVGLKDEDEVLENQIDKLTQLAYFKRFPTRQDSGMLPHQMLLALGPTNSPPKTAIDTVERLAWSANIVREGFEVPYVAYDSIVTPALETLRDYIVKWESNLYRAPYTSIVGPTMSGKTRLILELAKHIPVVYICLRPPNSTGQPPRSELADLMLPGRALQVDLKKRYTRLLHAIFRVVASFFSKPKRQHQAIQDQLNAWNKYSLQLNDAPVPFARDVQKEMEMLAHSPVKTTALQDAARKMYQSIPGQKEWLHCYWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GAG3</accession>
    <name>A0A180GAG3_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_08581</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000121">
        <property type="protein sequence ID" value="OAV89695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GAG3"/>
    <dbReference type="EnsemblFungi" id="PTTG_08581-t43_2">
        <property type="protein sequence ID" value="PTTG_08581-t43_2-p1"/>
        <property type="gene ID" value="PTTG_08581"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08581"/>
    <dbReference type="OrthoDB" id="10486286at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV89695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08581-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="504" mass="58583" checksum="B4015553201E99A1" modified="2016-09-07" version="1">MYSLREARSRERKESDHESLQFTMDPQSTDESQQRPIEADINEYKRFVTLHSCKENQQTEHFAVVSKVVGIERLRNYHDDLEEILKLKSPDTNQEDVISQWVKFFQGYKSSFTRLKNVLNLTDADHENQIKQLVELAHFIRFPTVKEPSGMSQEEMLTKIQSTKESRKSQEELTEFKRFKKLHVYPEEADKKFISIVEAAGVARLRKYHEDLEMCIESLEVGCQSSFRVYFKRFYQGASDSLRELKENVGLKDEDEVLENQIDKLTQLAYFKRFPTRQDSGMLPHQMLLALGPTNSPPKTAIDTVERLAWSANIVREGFEVPYVAYDSIVTPALETLRDYIVKWESNLYRAPYTSIVGPTMSGKTRLILELAKHIPVVYICLRPPNSTGQPPRSELADLMLPGRALQVDLKKRYTRLLHAIFRVVASFFSKPKRQHQAIQDQLNAWNKYSLQLNDAPVPFARDVQKEMEMLAHSPVKTTALQDAARKMYQSIPGQKEWLHCYWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GAK8</accession>
    <name>A0A180GAK8_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">L-type lectin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_28655</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000125">
        <property type="protein sequence ID" value="OAV89529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GAK8"/>
    <dbReference type="EnsemblFungi" id="PTTG_28655-t43_1">
        <property type="protein sequence ID" value="PTTG_28655-t43_1-p1"/>
        <property type="gene ID" value="PTTG_28655"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_28655"/>
    <dbReference type="OrthoDB" id="10265193at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07308">
        <property type="entry name" value="lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000434">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051136">
        <property type="entry name" value="Intracellular_Lectin-GPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005052">
        <property type="entry name" value="Lectin_leg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223:SF28">
        <property type="entry name" value="LECTIN, MANNOSE BINDING 1 LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223">
        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03388">
        <property type="entry name" value="Lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="L-type lectin-like" evidence="7">
        <location>
            <begin position="325"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="22"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="471"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OAV89529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_28655-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="549" mass="60530" checksum="C53D9F35FF068556" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GBH6</accession>
    <name>A0A180GBH6_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_07444</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000111">
        <property type="protein sequence ID" value="OAV90010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GBH6"/>
    <dbReference type="EnsemblFungi" id="PTTG_07444-t43_1">
        <property type="protein sequence ID" value="PTTG_07444-t43_1-p1"/>
        <property type="gene ID" value="PTTG_07444"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_07444"/>
    <dbReference type="OrthoDB" id="412109at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043124">
        <property type="term" value="P:negative regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038753">
        <property type="entry name" value="NFKBIL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15263">
        <property type="entry name" value="I-KAPPA-B-LIKE PROTEIN IKBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15263:SF1">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="166"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="166"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV90010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_07444-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="349" mass="40335" checksum="649011FF6ADAFBFB" modified="2016-09-07" version="1">MVVGKLRMKNTDDNHQSSQINKPRKRRQRTRSPSIDPPPEPYGPGNVMDEFPSGSSSGSRALKADKGKSRQQPGQAADDDFQTKLRDALNADNEDLRLESLLEDEVTLPRRWQDHQSRAHAHSDLNGHQLARLEPAEYEEYIRKRMWSQSHQKNYLYLKDQEEQKRKAREEAQQARKKQRADAKLRAERESLKQANQKLKSQQRCRDAYTRRWELLNYLLNSDGSKAETAGEGESGSRQKLKDEMGQVIEFEEIPWPVYPPSESDDPISAVERLNISSIREFLVGHLADDRSTAPARKKIIRAALLSYHPDRFDRLVHRVKDIDKAPQKAKEWGLRVSQVLNELNQTID</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GDJ9</accession>
    <name>A0A180GDJ9_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1,4-alpha-glucan-branching enzyme</fullName>
            <ecNumber evidence="4">2.4.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glycogen-branching enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PTTG_00048</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Glycogen-branching enzyme participates in the glycogen biosynthetic process along with glycogenin and glycogen synthase. Generates alpha-1,6-glucosidic branches from alpha-1,4-linked glucose chains, to increase solubility of the glycogen polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="4"/>
    <dbReference type="EMBL" id="ADAS02000095">
        <property type="protein sequence ID" value="OAV90725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GDJ9"/>
    <dbReference type="EnsemblFungi" id="PTTG_00048-t43_1">
        <property type="protein sequence ID" value="PTTG_00048-t43_1-p1"/>
        <property type="gene ID" value="PTTG_00048"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00048"/>
    <dbReference type="OrthoDB" id="196493at2759"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11321">
        <property type="entry name" value="AmyAc_bac_euk_BE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02854">
        <property type="entry name" value="E_set_GBE_euk_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000001">
        <property type="entry name" value="1,4-alpha-glucan branching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000080">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000250">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF3">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="11">
        <location>
            <begin position="150"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000463-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OAV90725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00048-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="669" mass="77242" checksum="06A730B1A598FA85" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GDL4</accession>
    <name>A0A180GDL4_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="3 9">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PTTG_09722</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="3 9"/>
    <dbReference type="EMBL" id="ADAS02000093">
        <property type="protein sequence ID" value="OAV90807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GDL4"/>
    <dbReference type="EnsemblFungi" id="PTTG_09722-t43_2">
        <property type="protein sequence ID" value="PTTG_09722-t43_2-p1"/>
        <property type="gene ID" value="PTTG_09722"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09722"/>
    <dbReference type="OrthoDB" id="60984at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000015">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 9" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="6 9" id="KW-0521">NADP</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="10">
        <location>
            <begin position="23"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="204"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OAV90807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09722-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="498" mass="56422" checksum="0069F5DCCC98AF11" modified="2016-09-07" version="1">MASDIPSCGGSNAGQLKENTIIVVLGASGDLAKKKTFPALFGLYFNGFLPAGTRIIGYARTKMEQEEFHKRVSQCIKAPIPAMKKKLEEYLGICSYVDGQYNEDASFQKLEEVIKEKEKDFKGSDRHRIFYMALPPSVFIDVANGLKKNNYSPNGFNRIVVEKPFGMDLPTSRQLMGNLKALWKEEETFRIDHYLGKEMVKNLLVLRFGNVILDASFNKNLISNVQITFKEPFGTEGRGGYFDEFGIIRDVMQNHLLQVLSLLTMERPVSFSAEDIRDEKVKILRFIPPIQREHSLLGQYTGADGKPGYLDDDTVPKGSICPTFAALVLYINSPRWEGVPFVLRAGKALNEQKTEIRIQYKDVTQGIFKDIARNELVIRVQPGEAVYLKMNAKAPGLAMKSVSTEMDLTYKRRFSDLKIPEAYEALILDAIKGDRSNFVRDDELEIAWKIFTPLLHYIDQEKPKPEPYAYGSRGPEGLDDFITKHAGYQRFTQGYKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GE14</accession>
    <name>A0A180GE14_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_12350</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000094">
        <property type="protein sequence ID" value="OAV90779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GE14"/>
    <dbReference type="EnsemblFungi" id="PTTG_12350-t43_2">
        <property type="protein sequence ID" value="PTTG_12350-t43_2-p1"/>
        <property type="gene ID" value="PTTG_12350"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12350"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV90779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12350-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="430" mass="49093" checksum="F305EDE521EAF326" modified="2016-09-07" version="1">MAESLSQPSLKSRNRKCAKEILFSINAVRTHPFKSWPRKSLLTLKDFNSAKLISYPGSLYVDLVTNEFERLKSKYYHPPRLGMPDETNINQAPSDISTDRKVPEGLANELQFILLPRLLEQICTLSSTLELPALLNEPESNLSLVLQLQADLECSLDRIQYLGATLWSITRSLRNRTNDHHLKGLKSFRLSRVKQSLPIMADDMYHLFDVANQLIQQTELSSVPCMAVCDSYSLEERLHWAVYFPWQLTRNIIERNMEFIIHRLNYPTYATKGENQLQRPVEFGDDTVIQLNKLMLPIMKLCVIFLGKFSSRGLAKERLPIFMEICSDQIEILGASADPMIPGLRRLLRKLRTADGPGGGVTSDDLTSLAKSLKSCLESPLPLIHMYFIPLIPDLNVQKHYTHWCETWNNLIALAIHNFTRVAESFVAIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GEQ5</accession>
    <name>A0A180GEQ5_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PTTG_08186</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PIAS family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000086">
        <property type="protein sequence ID" value="OAV91101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GEQ5"/>
    <dbReference type="EnsemblFungi" id="PTTG_08186-t43_1">
        <property type="protein sequence ID" value="PTTG_08186-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08186"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08186"/>
    <dbReference type="OrthoDB" id="28127at2759"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.780">
        <property type="entry name" value="PINIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023321">
        <property type="entry name" value="PINIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038654">
        <property type="entry name" value="PINIT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF4">
        <property type="entry name" value="TONALLI, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14324">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51466">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PINIT" evidence="11">
        <location>
            <begin position="179"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="SP-RING-type" evidence="10">
        <location>
            <begin position="384"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="122"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="485"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="143"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="494"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="544"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OAV91101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08186-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="592" mass="65104" checksum="8F3B1DF04C3EC693" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GER9</accession>
    <name>A0A180GER9_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GMC_OxRdtase_N domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_02175</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000093">
        <property type="protein sequence ID" value="OAV90822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GER9"/>
    <dbReference type="EnsemblFungi" id="PTTG_02175-t43_2">
        <property type="protein sequence ID" value="PTTG_02175-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02175"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02175"/>
    <dbReference type="OrthoDB" id="269227at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF138">
        <property type="entry name" value="DEHYDROGENASE PKFF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008109834" evidence="2">
        <location>
            <begin position="22"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="3">
        <location>
            <begin position="329"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV90822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02175-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="496" mass="54516" checksum="1317F14FBEC9D69C" modified="2016-09-07" version="1">MGFSFFIVCLLVSFMGGNVLSSNHQEHRRFDIPTLPVLGSIFEGKTYDYIIIGAGTAGMTLAARLSEDPDIHVAVVEAGIDYALSPANKPFVDTPGADTLGCGSKQDDAINDAIDWRFNTTPQSGANNRTVRYARGKTIGGSSARNFMVYQRASKGSLDKWAELTGDPEWSFEKRFVDYQKSVSFTAPKYDLRQESPRAQYDQLAFTQSHGPVQVSYPNMAQPFSKYMQLSMNEKGIPTSQDFNGGNLSGVQYASTTIDPQNGHRSSSRSFFTAARSRSNLVVYTTAMVKRITFDESAPPRAEGIEFIYTLTGLADKLLARKEVIVSAGAFQSPQLLMVSGIGPKDQLTANQIPIRVENPNVGQGMQDQLSSFNKDSSVHHCLALILCYSSNLIKFSIFFGPSYPVHSIETLTRVATRPDYLASQFLNFTLSQQGLLTNNVADMISFESFDISKLTQLNSHPLSAYPTDWPHVEYLSAAGVSGSPVLVYNNKRALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GF16</accession>
    <name>A0A180GF16_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_12146</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000086">
        <property type="protein sequence ID" value="OAV91129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GF16"/>
    <dbReference type="EnsemblFungi" id="PTTG_12146-t43_2">
        <property type="protein sequence ID" value="PTTG_12146-t43_2-p1"/>
        <property type="gene ID" value="PTTG_12146"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12146"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV91129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12146-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="236" mass="26397" checksum="D1D620B16937EA2F" modified="2016-09-07" version="1">MSQNGRGRPINRGGLSNRMEENRRRATGQQHLHTTMHLGFPSSRNSLALPSNTNYRPGSLNNSIPSETNRDSRSNTFPSVDNCNLAAQPWIPTNPTQPNHIQHLSRHIPVPFRPNSQTIHRDVSYASQMASSNKHPLPHHPNQRSSIASGGVAIMPPLFTSDQTQPLFPNTLPTAHSNRHILSPCPTTNQTPNNVFSHHSNHQDRLLSHRSQPASPSYQGRTMPHHRSQTCKFVDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GF20</accession>
    <name>A0A180GF20_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_28052</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000085">
        <property type="protein sequence ID" value="OAV91174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GF20"/>
    <dbReference type="EnsemblFungi" id="PTTG_28052-t43_1">
        <property type="protein sequence ID" value="PTTG_28052-t43_1-p1"/>
        <property type="gene ID" value="PTTG_28052"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_28052"/>
    <dbReference type="OrthoDB" id="1701769at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF149">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="4">
        <location>
            <begin position="17"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OAV91174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_28052-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="142" mass="15621" checksum="EA57D7B7777DA166" modified="2016-09-07" version="1">MIEKSKLTMINRSKQNLSDLLLMDQAIEMAKEALDANEIPVGCVLVSKTTEQVLSKGRNRTNETKNACLHAEFDAISGLHSIMSADKINWNDVKLYVTVEPCLMCSSALRQLGINLVYFGCSNDRFGGCGGVVSIHNDTVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GFP2</accession>
    <name>A0A180GFP2_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerase III subunit RPC10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">DNA-directed RNA polymerases III 12.5 kDa polypeptide</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">RNA polymerase III subunit C11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PTTG_27961</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="17">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000080">
        <property type="protein sequence ID" value="OAV91500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GFP2"/>
    <dbReference type="EnsemblFungi" id="PTTG_27961-t43_2">
        <property type="protein sequence ID" value="PTTG_27961-t43_2-p1"/>
        <property type="gene ID" value="PTTG_27961"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_27961"/>
    <dbReference type="OrthoDB" id="282152at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10509">
        <property type="entry name" value="Zn-ribbon_RPC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034014">
        <property type="entry name" value="Zn_ribbon_RPC11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="14">
        <location>
            <begin position="55"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00078207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OAV91500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_27961-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="99" mass="11608" checksum="24427217B37B30E0" modified="2016-09-07" version="1">MLVVSRATGENKFECTTCPYEYPIYRNYTDRTVLTRKEADDVLGGEDAWKNVDQTEAQCPKCENNRAFYMQLQIRSADEPMTTFYRCTVLQCSAQWRDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GFP4</accession>
    <name>A0A180GFP4_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ras-GAP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_06083</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
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                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000077">
        <property type="protein sequence ID" value="OAV91665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GG63"/>
    <dbReference type="EnsemblFungi" id="PTTG_12392-t43_1">
        <property type="protein sequence ID" value="PTTG_12392-t43_1-p1"/>
        <property type="gene ID" value="PTTG_12392"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12392"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV91665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12392-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="301" mass="33629" checksum="677492122291C336" modified="2016-09-07" version="1" fragment="single">MLRKIVYDHPADHMRRVDEVFPSKQEENLQGLRERVHPIETEGEEPRICDLASVPMDDFLEDESDMIHQFWTDPHGQNLDYLVTDFRQNQGSQSTLATPSSTLSCQTAGHAMGFDTSRLTHPSGKDLHSLVTDFRHDQGSRLTSSSTFSNCQTPGHVMGFETSRLTRPSFGSYPDSTITLQSPREQTGEHVKDIFPGYTGSQRFDVPDVAVNPAALSHSKSQAQCGSAPLQSWPDPVTTVHPLGPQGKNGLAYAAISLRNHLADTIGHFNHVKMAKLEPEFIQRELEIFAQLRDLEIKRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GGS2</accession>
    <name>A0A180GGS2_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_03749</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRP15 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000075">
        <property type="protein sequence ID" value="OAV91774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GGS2"/>
    <dbReference type="EnsemblFungi" id="PTTG_03749-t43_2">
        <property type="protein sequence ID" value="PTTG_03749-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03749"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03749"/>
    <dbReference type="OrthoDB" id="20949at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012459">
        <property type="entry name" value="Rrp15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13245">
        <property type="entry name" value="RRP15-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13245:SF14">
        <property type="entry name" value="RRP15-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07890">
        <property type="entry name" value="Rrp15p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="100"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="241"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV91774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03749-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="297" mass="32460" checksum="E8A6C692FC170921" modified="2016-09-07" version="1">MIRKVGTALMEAVTTTLKTRLTTSRTLTSTKTVGRVPAKFSLKIWERLWTSRMGCHGVRRHRGTLCFLFFFLISAERKRRARSPTSFGLMLSQALEASEKSNATGPSTTLHPATRQANRIAKQQAASESQRKQTAATRHELQERGHIKDVIGGWTPRPAVPFSEWLVQGDRKWSEEGAYVGGASQEKTLRRLAQTGVVKLFNAIRAAQNVDDEDAKASLTTLSGAQKRKLNKLVGDKLSKKAAENPDPAALADAPEGEPSVGRFKPNVLGSRGKDEALANLSKATFLELIKSGGKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GGS8</accession>
    <name>A0A180GGS8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_28052</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000085">
        <property type="protein sequence ID" value="OAV91173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GGS8"/>
    <dbReference type="EnsemblFungi" id="PTTG_28052-t43_3">
        <property type="protein sequence ID" value="PTTG_28052-t43_3-p1"/>
        <property type="gene ID" value="PTTG_28052"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_28052"/>
    <dbReference type="OrthoDB" id="1701769at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF149">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="2">
        <location>
            <begin position="17"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV91173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_28052-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="164" mass="18163" checksum="723F1C6ABC225DDE" modified="2016-09-07" version="1">MIEKSKLTMINRSKQNLSDLLLMDQAIEMAKEALDANEIPVGCVLVSKTTEQVLSKGRNRTNETKNACLHAEFDAISGLHSIIQLGINLVYFGCSNDRFGGCGGVVSIHNDPNLIHSRPLAALGGYRREEAIILLRKFYITENTNAPVPKKKTNRVLKMEIPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GGT9</accession>
    <name>A0A180GGT9_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_04701</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000085">
        <property type="protein sequence ID" value="OAV91183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GGT9"/>
    <dbReference type="EnsemblFungi" id="PTTG_04701-t43_2">
        <property type="protein sequence ID" value="PTTG_04701-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04701"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04701"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF282">
        <property type="entry name" value="WW DOMAIN-CONTAINING OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="286"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OAV91183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04701-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="311" mass="34479" checksum="93124049FC8BA764" modified="2016-09-07" version="1">MLCKTPKSTSWNLSDSPDLTGRVAIVTGGSTGTGYFISLELARKGAKVYLVCRNEERAQRAIISLRKELPDGSFDYIHFDLTRLRTCKDAASTFLKRENRLDILINNAAVSRCSSYSLSEDGIEIQACNTVGHFCLTERLVPLMKRTAQVADGRHARIVNITSASHKLNSEPDFASSQGLNQRRCSALERHGNSMLSIIVFNRELQRRLQGTGIICLAVHPGFISDQVLQSGQQETTRVDHAAYSPQRSQFPYRGGERCSLHTTVRCYCFGSGETGIGRVLLGTSRETSHTPSQCKRSDWNSRPKALGHMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GGV1</accession>
    <name>A0A180GGV1_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_27827</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000072">
        <property type="protein sequence ID" value="OAV91976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GGV1"/>
    <dbReference type="EnsemblFungi" id="PTTG_27827-t43_1">
        <property type="protein sequence ID" value="PTTG_27827-t43_1-p1"/>
        <property type="gene ID" value="PTTG_27827"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_27827"/>
    <dbReference type="OrthoDB" id="2502344at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV91976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_27827-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="244" mass="27948" checksum="4E5637C8422CE7B1" modified="2016-09-07" version="1">MRKISERQAIIRDLFMIHVLLHFEETNNLINNALNIPSIPTLGTMLYPNDASGRLVIDMLFEDEAEVGNILESVLSRRYLNERLPARTHDEFDLGRLFDMRPADFKQSVRTTKSGFVWLLNQIIDNAVFHNNSRRPQLPILHQLALTLEHLGSNGNGASVGRFARNLSVAHGTVVKMAQQYLCTNGRDSMARSTGIVKNIFNKRPDYMRLRQVYYLFSDRLAWIMRGQLCVSANVCSHRSRTAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GGV8</accession>
    <name>A0A180GGV8_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_27832</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTH2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="ADAS02000073">
        <property type="protein sequence ID" value="OAV91861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GGV8"/>
    <dbReference type="EnsemblFungi" id="PTTG_27832-t43_1">
        <property type="protein sequence ID" value="PTTG_27832-t43_1-p1"/>
        <property type="gene ID" value="PTTG_27832"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_27832"/>
    <dbReference type="OrthoDB" id="1733656at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000001">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF11">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="42"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV91861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_27832-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="213" mass="23227" checksum="E6D9E20D7854BCEB" modified="2016-09-07" version="1">MPHPTCRREVIPEPAFTYLALIISSLTIGYWVGLGRSLGYTRGNANHPRRTDSDSDDNLRRNEKPPGDSSESELDSHDEADLKSVQPSWSEESKLVLVVRGDLGMTKGKIAAQCSHATLACYKTLVKSNPALLRHWERSGQAKIALKLEKTECGTEEDEMLLLKAKARSLGLCAQDICDAGRTQIAAGSRTVLGIGPGPVGLINQVTRHLKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GH45</accession>
    <name>A0A180GH45_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PTTG_27812</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000072">
        <property type="protein sequence ID" value="OAV91941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GH45"/>
    <dbReference type="EnsemblFungi" id="PTTG_27812-t43_2">
        <property type="protein sequence ID" value="PTTG_27812-t43_2-p1"/>
        <property type="gene ID" value="PTTG_27812"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_27812"/>
    <dbReference type="OrthoDB" id="2496130at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF27">
        <property type="entry name" value="GH27708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OAV91941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_27812-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="41262" checksum="4C14AACB1FBD45AB" modified="2016-09-07" version="1">MCAQELLFRNAALQTTHKARLFLQALTCTSPLDKPTLTAGQDNQRHTKGDQNHNMNMAQLSEIDHSCPSRLARHVRSLQIEFNDICSLARGSSQWLCAIIRSCPRLENIAISSEVWDDCREPILEALSLIQEFAVLENYSLETVELSELKRRTGTVGKPIPTLNCTVRTMILHNPRLYERDLAILLTSFRQSIRSLTIEMSDPDHSMGRPGLCRILKDCTNPDLECLKVKTGRRFDDLADLMQSPSAIVDPDDPSSNPGLLDFVFKSPFALRKLKSLSFSGNLATNELFKFLPDSIVKLAWDNCEISLWALGSALSRRADKIILLPNLKCCSTRAPSWELTATLESELEARGGFFTRISRRCEAQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GH61</accession>
    <name>A0A180GH61_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_12415</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000083">
        <property type="protein sequence ID" value="OAV91303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GH61"/>
    <dbReference type="EnsemblFungi" id="PTTG_12415-t43_2">
        <property type="protein sequence ID" value="PTTG_12415-t43_2-p1"/>
        <property type="gene ID" value="PTTG_12415"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12415"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV91303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12415-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="209" mass="23367" checksum="1FCB4953DB1E906E" modified="2016-09-07" version="1" fragment="single">RDGQSICTNALASDCLPTPLIEGIEAKITRYVRIPSHRGARSPRLLGQSTENLSHHLKMDYGTESSLNPRGSCGFQTFLLGVLTCHTKTNLYILDGHWNDKKAIGFGTEIGDRSQFKKIFAKDPLINNAMSPFSKTGSDTLRALTTNFHSSSAHKRVATLAVFYHISEKPDPFIMEQFQILFHGRLLLHEGDLLGHIADVHRWLSPDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GHV3</accession>
    <name>A0A180GHV3_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_03911</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="ADAS02000066">
        <property type="protein sequence ID" value="OAV92326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_03911-t43_1">
        <property type="protein sequence ID" value="PTTG_03911-t43_1-p1"/>
        <property type="gene ID" value="PTTG_03911"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03911"/>
    <dbReference type="OrthoDB" id="2537092at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057948">
        <property type="entry name" value="TPR_TRIP12_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25579">
        <property type="entry name" value="TPR_TRIP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="1478"/>
            <end position="1858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="572"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="756"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="804"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1080"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1305"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="756"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="807"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1080"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1135"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1153"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1216"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="1825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OAV92326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03911-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="1858" mass="201068" checksum="03AB5843E121E24D" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GHY0</accession>
    <name>A0A180GHY0_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MFS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_02245</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000065">
        <property type="protein sequence ID" value="OAV92357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GHY0"/>
    <dbReference type="EnsemblFungi" id="PTTG_02245-t43_2">
        <property type="protein sequence ID" value="PTTG_02245-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02245"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02245"/>
    <dbReference type="OrthoDB" id="2502798at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000018">
        <property type="entry name" value="MFS transporter permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF85">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G00710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="48"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OAV92357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02245-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="333" mass="37373" checksum="F1F08748DC3514AC" modified="2016-09-07" version="1" fragment="single">MDIRDDKNAQLDSETSDHFMREEEKSHSIPAIKTPQQKRALLKLDLLLIPILGLFYFLSFLDRSNLGNVRIIGLQKDLKMTDYDFSMALTITFIPYILIELPSNLLVKRIGAGILIPLIVTIWGLITCLQGLVTSYQGLLVSRFFLGLVEGGLYPAIILYLSSFYTRTELQFRIALFWGTICIAGAASGLLTFEIIKLHGRWGHPGWAWVFLIEGFVTAVFGILGFFVLPSRIERVWILTEAEKAHLLSRLETASSSSSSLTLSNSFIENYPPKSTGRQVWEACKSPHVVLLNVAQFACAGNIYSLAYFTPTIVNSVSFYRPSRECCLLQTDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GI58</accession>
    <name>A0A180GI58_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PTTG_02235</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000065">
        <property type="protein sequence ID" value="OAV92370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GI58"/>
    <dbReference type="EnsemblFungi" id="PTTG_02235-t43_2">
        <property type="protein sequence ID" value="PTTG_02235-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02235"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02235"/>
    <dbReference type="OrthoDB" id="2498368at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF81">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="83"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="152"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="684"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="923"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="236"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="685"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="926"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="980"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1011"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1037"/>
            <end position="1047"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OAV92370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02235-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="1151" mass="128284" checksum="4E00A17E9B09B069" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GI59</accession>
    <name>A0A180GI59_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Lysophospholipase NTE1</fullName>
            <ecNumber evidence="3 16">3.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Intracellular phospholipase B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PTTG_03931</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="21 22" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="21">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="21" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 16">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the NTE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="3 16"/>
    <dbReference type="EMBL" id="ADAS02000066">
        <property type="protein sequence ID" value="OAV92301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_03931-t43_2">
        <property type="protein sequence ID" value="PTTG_03931-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03931"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03931"/>
    <dbReference type="OrthoDB" id="421051at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046470">
        <property type="term" value="P:phosphatidylcholine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000062">
        <property type="entry name" value="Lysophospholipase NTE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000007">
        <property type="entry name" value="Lysophospholipase NTE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000018">
        <property type="entry name" value="Lysophospholipase NTE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001423">
        <property type="entry name" value="LysoPLipase_patatin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056556">
        <property type="entry name" value="NTE1_P-loop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF29">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE SWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24179">
        <property type="entry name" value="NTE_Ploop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01237">
        <property type="entry name" value="UPF0028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 15 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 15 16" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="11 15 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="715"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="907"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="19">
        <location>
            <begin position="1304"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="210"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="555"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="621"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="690"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="764"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="15">
        <location>
            <begin position="1308"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="15">
        <location>
            <begin position="1335"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="15">
        <location>
            <begin position="1455"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="271"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="299"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="347"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="640"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="701"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="713"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="839"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="15">
        <location>
            <position position="1337"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="1455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OAV92301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03931-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="1525" mass="170070" checksum="91742D84521F1B86" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GI86</accession>
    <name>A0A180GI86_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Clustered mitochondria protein homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Protein TIF31 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CLU1</name>
        <name evidence="3" type="synonym">TIF31</name>
        <name evidence="6" type="ORF">PTTG_03716</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="8">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
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    <reference evidence="7" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
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        <text evidence="3">May associate with the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
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        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
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        <property type="gene ID" value="PTTG_03716"/>
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    <dbReference type="OrthoDB" id="771227at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="CLU-central"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Hypothetical protein (hspc210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CLU-central"/>
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        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CLU"/>
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        <property type="entry name" value="eIF3_p135"/>
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        <location>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03013"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51823"/>
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        <source>
            <dbReference type="EMBL" id="OAV92189.1"/>
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        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03716-t43_1-p1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
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    <sequence length="1605" mass="176831" checksum="22CB835AC64DB857" modified="2016-09-07" version="1"> </sequence>
</entry>
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        <recommendedName>
            <fullName evidence="5">BZIP domain-containing protein</fullName>
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        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
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    <reference evidence="2" key="1">
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                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
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    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="OAV92581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="PTTG_04492"/>
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    <dbReference type="OrthoDB" id="2503715at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        </location>
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        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04492-t43_2-p1"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="2">DUF7918 domain-containing protein</fullName>
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        <name evidence="3" type="ORF">PTTG_05005</name>
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    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000061">
        <property type="protein sequence ID" value="OAV92625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GIZ8"/>
    <dbReference type="EnsemblFungi" id="PTTG_05005-t43_2">
        <property type="protein sequence ID" value="PTTG_05005-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05005"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05005"/>
    <dbReference type="OrthoDB" id="3364132at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057678">
        <property type="entry name" value="DUF7918"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223:SF5">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25534">
        <property type="entry name" value="DUF7918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7918" evidence="2">
        <location>
            <begin position="96"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="289"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV92625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05005-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="334" mass="36703" checksum="4D20116C34E2DA56" modified="2016-09-07" version="1" fragment="single">MPTNEASGASCAISLVPTKTDDEPETTATEATACKEYCHESSLDPITHVFKEVVTIESTNSAQFKIHLDIKPTACCVLEKTATNQCRRTKARKSLQPHDYLVQFIFDGIKTSTAKLLRTQTGRQTFSRVNSVDRAVSRPFQFAPIKLVDPDDPAEEICQNEEIIKSLGTIEIQIFKCKLVSDKSPAPLAGSSLAKSTNQMSFSESNKKARLACTAGLGEPTINERTDCPTYIVKHKAVFPFLHVRLVFLIITPTPRLSTSIQFVFRYKPRSILIAEKIIPNPVVPTEPPATASSTASTSKPKPPPKSITIDSDSEPENNYAETSKSKKTNTDTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GJ66</accession>
    <name>A0A180GJ66_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycoside hydrolase family 5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_05863</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000063">
        <property type="protein sequence ID" value="OAV92478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GJ66"/>
    <dbReference type="EnsemblFungi" id="PTTG_05863-t43_3">
        <property type="protein sequence ID" value="PTTG_05863-t43_3-p1"/>
        <property type="gene ID" value="PTTG_05863"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05863"/>
    <dbReference type="OrthoDB" id="9971853at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050295">
        <property type="term" value="F:steryl-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904462">
        <property type="term" value="P:ergosteryl 3-beta-D-glucoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041036">
        <property type="entry name" value="GH5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052066">
        <property type="entry name" value="Glycosphingolipid_Hydrolases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308:SF5">
        <property type="entry name" value="ERGOSTERYL-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18564">
        <property type="entry name" value="Glyco_hydro_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="687"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="5">
        <location>
            <begin position="78"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5 C-terminal" evidence="6">
        <location>
            <begin position="577"/>
            <end position="670"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV92478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05863-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="713" mass="80899" checksum="3B0FDEEBFE42B9B4" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GJ67</accession>
    <name>A0A180GJ67_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycoside hydrolase family 5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PTTG_05863</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000063">
        <property type="protein sequence ID" value="OAV92479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GJ67"/>
    <dbReference type="EnsemblFungi" id="PTTG_05863-t43_1">
        <property type="protein sequence ID" value="PTTG_05863-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05863"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05863"/>
    <dbReference type="OrthoDB" id="9971853at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050295">
        <property type="term" value="F:steryl-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904462">
        <property type="term" value="P:ergosteryl 3-beta-D-glucoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052066">
        <property type="entry name" value="Glycosphingolipid_Hydrolases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308:SF5">
        <property type="entry name" value="ERGOSTERYL-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="5">
        <location>
            <begin position="78"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OAV92479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05863-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="579" mass="66107" checksum="B23F8C5F79BD64C3" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GJ74</accession>
    <name>A0A180GJ74_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_12698</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000060">
        <property type="protein sequence ID" value="OAV92705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GJ74"/>
    <dbReference type="EnsemblFungi" id="PTTG_12698-t43_1">
        <property type="protein sequence ID" value="PTTG_12698-t43_1-p1"/>
        <property type="gene ID" value="PTTG_12698"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12698"/>
    <dbReference type="OrthoDB" id="2500617at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV92705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12698-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="184" mass="20880" checksum="A4A7420280A86C99" modified="2016-09-07" version="1">MSQEMMDINLLLGIRRSSRPLILSDFSIGDAEEANGTSISRTDEHAEFNGPKSLERIRRLTETILSVDRPRKLSGMISEDPFSGKIPEFKFLRLLQVNQPPDPPSWFQWLNHIDVDQDLDKITLDSVPDDVLQAEQWASLALQPPQHRSPSLHPASLRLSALKKPTKRSSSEISHKKRPYTCNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GJ78</accession>
    <name>A0A180GJ78_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Potassium transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_06393</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000063">
        <property type="protein sequence ID" value="OAV92489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GJ78"/>
    <dbReference type="EnsemblFungi" id="PTTG_06393-t43_1">
        <property type="protein sequence ID" value="PTTG_06393-t43_1-p1"/>
        <property type="gene ID" value="PTTG_06393"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06393"/>
    <dbReference type="OrthoDB" id="9999863at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140107">
        <property type="term" value="F:high-affinity potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051143">
        <property type="entry name" value="TrkH_K-transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064:SF30">
        <property type="entry name" value="HIGH-AFFINITY POTASSIUM TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064">
        <property type="entry name" value="POTASSIUM TRANSPORT PROTEIN DDB_G0292412-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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            <strain evidence="8">1-1 BBBD Race 1</strain>
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    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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            <strain evidence="8">1-1 BBBD Race 1</strain>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
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            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
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        <source>
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            <dbReference type="EnsemblFungi" id="PTTG_02523-t43_1-p1"/>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000056">
        <property type="protein sequence ID" value="OAV92975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_02796-t43_1">
        <property type="protein sequence ID" value="PTTG_02796-t43_1-p1"/>
        <property type="gene ID" value="PTTG_02796"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02796"/>
    <dbReference type="OrthoDB" id="1043025at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038066">
        <property type="term" value="P:p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06626">
        <property type="entry name" value="STKc_MEKK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF32">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1056"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="287"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1361"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="289"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="304"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1365"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="1085"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV92975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02796-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="1422" mass="160812" checksum="45EC5E49299FDC5C" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GK89</accession>
    <name>A0A180GK89_PUCT1</name>
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        <submittedName>
            <fullName evidence="11">NADPH-dependent diflavin oxidoreductase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PTTG_05597</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="ADAS02000053">
        <property type="protein sequence ID" value="OAV93206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GK89"/>
    <dbReference type="EnsemblFungi" id="PTTG_05597-t43_2">
        <property type="protein sequence ID" value="PTTG_05597-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05597"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05597"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000013">
        <property type="entry name" value="NADPH-dependent diflavin oxidoreductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000034">
        <property type="entry name" value="NADPH-dependent diflavin oxidoreductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF10">
        <property type="entry name" value="NADPH-DEPENDENT DIFLAVIN OXIDOREDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="8">
        <location>
            <begin position="16"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="9">
        <location>
            <begin position="205"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV93206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05597-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="446" mass="51198" checksum="01486F63E2867828" modified="2016-09-07" version="1">MAIEQGPSITTKERRLLVLYASQTGTAEDVAQQISRGARRLHFNTELASMDGYDRDKIFDEDLVIFVCSTTGQGVQPQNMTKFWNALKLASLPTDFIDNIRFTIFGLGDSSYPKFNWVTKKLFRRLAQIGAQSFYERGEADDQNPNGIDSTLLPWLDGLWKKLLEIKPLPNHLTPIPPTTCLPPSFRLQPASEPIPKLLPTPPPPDLLSATLTKNQRLTAPDHWQDTRHLEFESDQTLEFEPGSVCEILPENCPEEVDKLFKLMKWTDLSDQVYKLTRCDSNQDLPVGWNLYATLTEIFTTRLDFMAVPGRSFIDWLSHFTSDPMETERLQEFCSIEGQDDLFEYTRRPRRTILEVLSEFKSASIPLDYIHDVFPQIRPRLFSIASSPKLFPNQIQLLVAVVNYKTRLSVPRKGLCTSWLSKLEIVFRHSNTDPDQPRIRDFSERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GK95</accession>
    <name>A0A180GK95_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">21S rRNA pseudouridine(2819) synthase</fullName>
            <ecNumber evidence="7">5.4.99.43</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Pseudouridine synthase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Pseudouridylate synthase PUS5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Uracil hydrolyase PUS5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PTTG_02223</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="15">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Pseudouridylate synthase responsible for the pseudouridine-2819 formation in mitochondrial 21S rRNA. May modulate the efficiency or the fidelity of the mitochondrial translation machinery.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>uridine(2819) in 21S rRNA = pseudouridine(2819) in 21S rRNA</text>
            <dbReference type="Rhea" id="RHEA:42556"/>
            <dbReference type="Rhea" id="RHEA-COMP:10113"/>
            <dbReference type="Rhea" id="RHEA-COMP:10114"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.43"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.43" evidence="7"/>
    <dbReference type="EMBL" id="ADAS02000065">
        <property type="protein sequence ID" value="OAV92383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GK95"/>
    <dbReference type="EnsemblFungi" id="PTTG_02223-t43_1">
        <property type="protein sequence ID" value="PTTG_02223-t43_1-p1"/>
        <property type="gene ID" value="PTTG_02223"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02223"/>
    <dbReference type="OrthoDB" id="428658at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160143">
        <property type="term" value="F:21S rRNA pseudouridine(2819) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF81">
        <property type="entry name" value="21S RRNA PSEUDOURIDINE(2819) SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="13">
        <location>
            <begin position="129"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="45"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OAV92383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02223-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="275" mass="30981" checksum="E21530E8FECA8C3B" modified="2016-09-07" version="1">MASKSLHSTLRSSLSRKTSCLLIRHVAITSRSTQANAQVPIGSTLVKRPSQSSHPATRSIPSQTPPSKLLPVGEPVQRALTRTKKIQTKEKPLSEEEKENQRIQREVELTAQLEKSFRPPKILFENEKVVVIDKPAGCALQADPGSEAYIRWKLIMDYLEKRRGKLFIVHRLDKSTTGPLILAKSPTASRTLSTQFKNSTIKKIYYAAIQFLGDRIGPIQDAKSSGQIECRMRDRNDRMVIAEVKPCNRQIETAHRSETSTPATLFKILGWADRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GND2</accession>
    <name>A0A180GND2_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Poly A polymerase head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_07644</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000040">
        <property type="protein sequence ID" value="OAV94316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GND2"/>
    <dbReference type="EnsemblFungi" id="PTTG_07644-t43_2">
        <property type="protein sequence ID" value="PTTG_07644-t43_2-p1"/>
        <property type="gene ID" value="PTTG_07644"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_07644"/>
    <dbReference type="OrthoDB" id="2499715at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CC tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3090.10">
        <property type="entry name" value="cca-adding enzyme, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734:SF5">
        <property type="entry name" value="CCA TRNA NUCLEOTIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734">
        <property type="entry name" value="TRNA-NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81891">
        <property type="entry name" value="Poly A polymerase C-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly A polymerase head" evidence="6">
        <location>
            <begin position="172"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA nucleotidyltransferase/poly(A) polymerase RNA and SrmB- binding" evidence="7">
        <location>
            <begin position="377"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OAV94316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_07644-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="523" mass="59277" checksum="88839B0285DE530A" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GND4</accession>
    <name>A0A180GND4_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuolar protein sorting-associated protein 33A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_08324</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000044">
        <property type="protein sequence ID" value="OAV93978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GND4"/>
    <dbReference type="EnsemblFungi" id="PTTG_08324-t43_1">
        <property type="protein sequence ID" value="PTTG_08324-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08324"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08324"/>
    <dbReference type="OrthoDB" id="10262287at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043155">
        <property type="entry name" value="VPS33_dom3b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV93978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08324-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="58011" checksum="EA38964718226C9C" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GQH5</accession>
    <name>A0A180GQH5_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_05154</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000038">
        <property type="protein sequence ID" value="OAV94639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GQH5"/>
    <dbReference type="EnsemblFungi" id="PTTG_05154-t43_2">
        <property type="protein sequence ID" value="PTTG_05154-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05154"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05154"/>
    <dbReference type="OrthoDB" id="2500126at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV94639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05154-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="196" mass="22336" checksum="C22CC803844B8BBC" modified="2016-09-07" version="1">MPHPSKSSRITPSKQTRKTSSNFGQASPSHDKHETDSLESVKRELIHAIETFSKFRQEWDDLVLNKSLNLIIKIMELEAEIRSTVENSCFLQKCAEDQVVLEDGYAWKQFCKMNGHCSELEARLGDMTKLESKIQKANDHFEELVQKSCASHGMKTIFDTPVGLTWPLPRFCKVITSSVYRATTSIHSNSIIPYVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GQH6</accession>
    <name>A0A180GQH6_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phos_pyr_kin domain-containing protein</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="2">Phosphomethylpyrimidine kinase, variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_00623</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000042">
        <property type="protein sequence ID" value="OAV94213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GQH6"/>
    <dbReference type="EnsemblFungi" id="PTTG_00623-t43_2">
        <property type="protein sequence ID" value="PTTG_00623-t43_2-p1"/>
        <property type="gene ID" value="PTTG_00623"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00623"/>
    <dbReference type="OrthoDB" id="10028886at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858:SF17">
        <property type="entry name" value="HYDROXYMETHYLPYRIMIDINE_PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV94213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00623-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="204" mass="22223" checksum="932B7A172F6F7675" modified="2016-09-07" version="1">MLHSPEIIDAIAASLSRFYPDPTNQPHLVIDPVMVSTSGHDLLQGGGLEMLIGNLFCRASLVTPNLPEALLLSGRKSRNQTIESIQDMQDCCREIAQLGARNVLLKGGHILMSNEMVTDLLYQADSNSFQRFTHQRLETPNTHGTGCTLSAALAVYLAKGFSLIDAVESAIKYVEGGMKHSFALGNGSGPLNHFHNIQQRILPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GQJ9</accession>
    <name>A0A180GQJ9_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_06891</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000037">
        <property type="protein sequence ID" value="OAV94669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GQJ9"/>
    <dbReference type="EnsemblFungi" id="PTTG_06891-t43_2">
        <property type="protein sequence ID" value="PTTG_06891-t43_2-p1"/>
        <property type="gene ID" value="PTTG_06891"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06891"/>
    <dbReference type="OrthoDB" id="2498269at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055334">
        <property type="entry name" value="PEX8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214:SF1">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV94669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06891-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="447" mass="49732" checksum="3E06FD00AA3EA489" modified="2016-09-07" version="1">MENVDDGAIYIDVMTWLLSPSQDGNADDDQDERMLENLCRLMVHLRLALVDQLMMAVLHSAKLWSLGIPGKASTDVPSAGKDSSMKNKAVEPFMSIFLKLKAACLAKFHRSSLPHSNHSTFPSIPGPDHLNQTHPKLKPSTIKFIDRWRAIVVGAANENSRDGHFTPFDLRQLCLMAVLLVSTKSFDISSSSFRLQSLIEQDCIKCIKRSIRQTQLADSTVQAITHFDPDSVITWLAVQIIPSINPLHLAESPTNVIINHFAKSLFQVFENGHLFDQLSTSLDLAPHSLSLHPSSSTDQGLTRTSSDPLAQVVLSISLSLSKLIEHTTKTSDVKSFSADLRSLVAVTEKIHESWRTATSAYDQVHLPEFKENSVSINHLKPEENQADRWLRNIFFCCLLILGGLVNKLAVRGFSQVMAENLFPPMFQILSPRRTCKPHSEGISALPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GRJ4</accession>
    <name>A0A180GRJ4_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="8">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PTTG_03801</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="8"/>
    <dbReference type="EMBL" id="ADAS02000038">
        <property type="protein sequence ID" value="OAV94583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GRJ4"/>
    <dbReference type="EnsemblFungi" id="PTTG_03801-t43_1">
        <property type="protein sequence ID" value="PTTG_03801-t43_1-p1"/>
        <property type="gene ID" value="PTTG_03801"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03801"/>
    <dbReference type="OrthoDB" id="3264224at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
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        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF20">
        <property type="entry name" value="[3-METHYL-2-OXOBUTANOATE DEHYDROGENASE [LIPOAMIDE]] KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="9">
        <location>
            <begin position="142"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV94583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03801-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="311" mass="33966" checksum="5037CCC123F70FE0" modified="2016-09-07" version="1">MSIYRHSLEEINSLPPIRTVADNAALLQALVTMVDDAADVIGMFATGFKESKRYLSEEQITSFLDRAIQSRISIRLIAEQHLSLSKAEHSPNGSRLGIVDRKVKLKKTLESVLEFAGELCEGTFGIAPEWTILGEVEAEVCFVEMHLQYILIEVIKNALRATIEHHREVTKSTNPPLPPIEVTLAIERPSPVALNSNDAAAQVSQSILCIRIRDRGGGIDPKDLPHVFSYAFSTVGSEEKAQNGMPNQADFGRISYGQEALKSSLGRIAGLGFGLPMARLYCRYFGGNLELVNMHGIGGGVDSYITVVVGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GRL1</accession>
    <name>A0A180GRL1_PUCT1</name>
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        <submittedName>
            <fullName evidence="3">ACPS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_03814</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000038">
        <property type="protein sequence ID" value="OAV94603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GRL1"/>
    <dbReference type="EnsemblFungi" id="PTTG_03814-t43_2">
        <property type="protein sequence ID" value="PTTG_03814-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03814"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2507452at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV94603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03814-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="175" mass="19455" checksum="0A55202430348F26" modified="2016-09-07" version="1">MHDYHHLRQITERIIGTGIDILDRRRMARMVAGDTACASAPDAQRLARLSRRILSGAELDCPEFKLAASFSDLWSADMVGFLANRNRWTAKEAAYKALYPYHEARWKELSLLKGQPGQPKKPVLIFHPHSHITLHVSLSHDGPYTIASVLATSSSSPDSPGHHTITTGRTHSVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GRL5</accession>
    <name>A0A180GRL5_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ULP_PROTEASE domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_26810</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000034">
        <property type="protein sequence ID" value="OAV94922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GRL5"/>
    <dbReference type="EnsemblFungi" id="PTTG_26810-t43_4">
        <property type="protein sequence ID" value="PTTG_26810-t43_4-p1"/>
        <property type="gene ID" value="PTTG_26810"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_26810"/>
    <dbReference type="OrthoDB" id="2496817at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF141">
        <property type="entry name" value="GH15225P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="123"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV94922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_26810-t43_4-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="343" mass="39104" checksum="1F1B764D6D550F74" modified="2016-09-07" version="1">MAFQNPPPRMNDEDDESDDPIDMLSSKSRTRSYVKAGSPRAPSQSLLNPTTDFPIDKNHAVTSSSQGLDLLSSHFGQLHLSQINSRTKPHQRQWPTSLTPAQQLIVDKALRPNNETIYDLPGASCGIKELQILSLPAQWINDEIINYYGSLINLQSQNQRSLKALDVHCFSSFFMSQFDLGGHQNVQRWTRKINLFQKDLILFPINLSNLHWVLGVINIQQKRFEYYDSLGGSNSKVLLKLRRYYKDEYQTKNSDNADLAQWTDYQPEVPLQSNSSDCGVFVCQFMHSLSQNSIHQDAQEPMFDFTAKNMPYLRQKMILEIISKAFLPDNSPESSNQAGTLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GSI2</accession>
    <name>A0A180GSI2_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_09036</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000028">
        <property type="protein sequence ID" value="OAV95511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GSI2"/>
    <dbReference type="EnsemblFungi" id="PTTG_09036-t43_2">
        <property type="protein sequence ID" value="PTTG_09036-t43_2-p1"/>
        <property type="gene ID" value="PTTG_09036"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09036"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV95511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09036-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="376" mass="42736" checksum="BF96E8904F4C2E13" modified="2016-09-07" version="1">MVDELVGRNEVIDKLESILLPSIKDRITSFLLALDLQNLGKQLDANLDSIPELTSEINQILAKTTHAVESVSAPFEGSEYSDGRNLGRCKEYRCRLLCAKVKDIMSEHLARLFARSGHFLEYWELSRKDPGDSWGRQEMVKRAETASRIADTTFRLIDQTIEWSKKSDLAILQEHWSEAEEKVNGALEDLTQLAADPSIGSGQAAMDDEMTPRNRISRLARLSIPIVKLTRIFLNKITKTTPKKPLFTLDPEINSKSLELLSEGPTNIARHLEYIAKFLAEQNQRNARPNIARDHNSSELLQIPKLFQSTLLLLSFYLIPLPTTAVDHHSVSRQHFKSWFHYLVSPYLEATDRMLDALFDSQGFDLQNLSLLTNHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GSI3</accession>
    <name>A0A180GSI3_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ULP_PROTEASE domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_26810</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000034">
        <property type="protein sequence ID" value="OAV94923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GSI3"/>
    <dbReference type="EnsemblFungi" id="PTTG_26810-t43_3">
        <property type="protein sequence ID" value="PTTG_26810-t43_3-p1"/>
        <property type="gene ID" value="PTTG_26810"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_26810"/>
    <dbReference type="OrthoDB" id="2496817at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF141">
        <property type="entry name" value="GH15225P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="118"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV94923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_26810-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="338" mass="38626" checksum="CD7468F879430DD8" modified="2016-09-07" version="1">MAFQNPPPTGMNDEDDESDDPIDMLSSKSRTRSYVKLAFQLLNPTTDFPIDKNHAVTSSSQGLDLLSSHFGQLHLSQINSRTKPHQRQWPTSLTPAQQLIVDKALRPNNETIYDLPGASCGIKELQILSLPAQWINDEIINYYGSLINLQSQNQRSLKALDVHCFSSFFMSQFDLGGHQNVQRWTRKINLFQKDLILFPINLSNLHWVLGVINIQQKRFEYYDSLGGSNSKVLLKLRRYYKDEYQTKNSDNADLAQWTDYQPEVPLQSNSSDCGVFVCQFMHSLSQNSIHQDAQEPMFDFTAKNMPYLRQKMILEIISKAFLPDNSPESSNQAGTLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GSJ3</accession>
    <name>A0A180GSJ3_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="10">TPR_REGION domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_04844</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NASP family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000026">
        <property type="protein sequence ID" value="OAV95730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GSJ3"/>
    <dbReference type="EnsemblFungi" id="PTTG_04844-t43_3">
        <property type="protein sequence ID" value="PTTG_04844-t43_3-p1"/>
        <property type="gene ID" value="PTTG_04844"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04844"/>
    <dbReference type="OrthoDB" id="2505766at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051730">
        <property type="entry name" value="NASP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019544">
        <property type="entry name" value="Tetratricopeptide_SHNi-TPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15081:SF1">
        <property type="entry name" value="NUCLEAR AUTOANTIGENIC SPERM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15081">
        <property type="entry name" value="NUCLEAR AUTOANTIGENIC SPERM PROTEIN NASP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10516">
        <property type="entry name" value="SHNi-TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="263"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide SHNi-TPR" evidence="8">
        <location>
            <begin position="264"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="140"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="348"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="444"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="356"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OAV95730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04844-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="488" mass="51788" checksum="A96B0734C39A87F6" modified="2016-09-07" version="1">MFRSKPRVRSRPPDAGLGHTLDGPFQNSTKQSQTMSDSFNQPSEDPKGKQEQVAAAQSEEANDTDIDTIEAELAAGARAFAFKNYAIATECFSKACEKLTTHHPENHTAIIDAHLSYGKALLRNAITLGSLSGGLNAHNPAAAATEPGDAAPEVTESAASSSKGTANQAGAASSSKPSQPVAKGNKGNPQIHFSGDSSDEDDDEGAGEDGDEEIDQAEEPVTVEDELENAFQVLEFARQSILSQVDSSKDQPEQAEKLQNKLIDVYDLIAQVHQEGEQFEQAVESYKSSLEVKKSRTDVTAGAIAETHLMIALALELIPDEGESMKQAIGHVDEAIELMKAQLAKLTEQAGGQTEEGGKEKPTESSEGTKQDVGPANKLKSEIEETQSLIGELEAKREELKTVPQAPAMTEADKALQAYLAAVNNNPSTANGKAAINDLTSLVKKRARPVDSSSDPQPNTSSSSSALPSTTDTNLPPETPSKKIKTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GSK4</accession>
    <name>A0A180GSK4_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="12">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PTTG_03654</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000030">
        <property type="protein sequence ID" value="OAV95292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GSK4"/>
    <dbReference type="EnsemblFungi" id="PTTG_03654-t43_1">
        <property type="protein sequence ID" value="PTTG_03654-t43_1-p1"/>
        <property type="gene ID" value="PTTG_03654"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03654"/>
    <dbReference type="OrthoDB" id="421009at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15844">
        <property type="entry name" value="SNARE_syntaxin5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021538">
        <property type="entry name" value="Syntaxin-5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF3">
        <property type="entry name" value="SYNTAXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11416">
        <property type="entry name" value="Syntaxin-5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="326"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="10">
        <location>
            <begin position="254"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OAV95292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03654-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="345" mass="38530" checksum="EEE37E4E3B1E39EE" modified="2016-09-07" version="1">MDRTQEFKSCVASIRSRTASQLVPEHKQRLLDNSSPNRKNQRAPGARGEFARLAGMIGKDIQQTTVKLSQLAQLAKRKTLFDDRPVEISELTYIIKQDIAQLNQQIAQLQTFVKQNLSSNRGQKQPVDEHNNNVVMMLQSKLADTSMGFKDVLEIRTQNMKATRDRTEQFQFNTPGLATASQSDSPLYAAQQAGGVASAVNRTLYDSKGKGKSTQEGYHQNEYLALDMGKNSNPAESGGPQGYMQMQLAQDNSDAYLQQRSTAIESIESTITELGSIFSQLATMVAQQGEQVQRIDQDTIDIESNIQSAQSELLKFYSSISGNRMLMFKVFGMIMIFFLLFVLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GTJ6</accession>
    <name>A0A180GTJ6_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription regulator Rua1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_02101</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000022">
        <property type="protein sequence ID" value="OAV96075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GTJ6"/>
    <dbReference type="EnsemblFungi" id="PTTG_02101-t43_3">
        <property type="protein sequence ID" value="PTTG_02101-t43_3-p1"/>
        <property type="gene ID" value="PTTG_02101"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02101"/>
    <dbReference type="OrthoDB" id="5595379at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028012">
        <property type="entry name" value="Rua1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28125">
        <property type="entry name" value="MEIOTIC EXPRESSION UP-REGULATED PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28125:SF2">
        <property type="entry name" value="MEIOTIC EXPRESSION UP-REGULATED PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14616">
        <property type="entry name" value="Rua1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator Rua1 C-terminal" evidence="2">
        <location>
            <begin position="670"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="847"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="856"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="907"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="954"/>
            <end position="966"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV96075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02101-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="966" mass="105650" checksum="12C36271612AAF9F" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GUR8</accession>
    <name>A0A180GUR8_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_12057</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
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                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
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                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
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                <person name="Berlin A."/>
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                <person name="Howarth C."/>
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    </evidence>
    <sequence length="388" mass="42605" checksum="015D704A0D3EE41A" modified="2016-09-07" version="1">MYMYMQDGSRRDTRGTPLPLQPSRTTTNRENQGLNSSMDRRKRRRNPTKNKIPTPAHPEDNHHEDETLDPGVGPPQSEFIRSLPPSSLIKYLNRHCLLNSPFQSAPSSSASATAATSAPPLPTSSKKQLVDDYNRFALLLSSSRHPNQTTQRSGTTEDRARSIYDMDIKPAQTEDQSSSISQSTSALSSGGGSSGSSSPGLACSTRSSSSFYRKLFGPAALLDDDEHEDQPARKKQAKADRAGPRDSLFSFDDNLTQLVLGHHHQSFPSLLVSPSQPAGSIQPRQRPSSPTSSPSAAGPRRHLSRPGPDDHELLFGPLPDSLHRPSLKRKSIQKTATLNHHGFVFNIHLNHLPSILHPHPPSDHIRLIEDHVLSNFVYSVKTRETSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GV17</accession>
    <name>A0A180GV17_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PABS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_02957</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000018">
        <property type="protein sequence ID" value="OAV96656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GV17"/>
    <dbReference type="EnsemblFungi" id="PTTG_02957-t43_1">
        <property type="protein sequence ID" value="PTTG_02957-t43_1-p1"/>
        <property type="gene ID" value="PTTG_02957"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02957"/>
    <dbReference type="OrthoDB" id="2016285at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037959">
        <property type="entry name" value="MFS_SpdSyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43317">
        <property type="entry name" value="THERMOSPERMINE SYNTHASE ACAULIS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43317:SF1">
        <property type="entry name" value="THERMOSPERMINE SYNTHASE ACAULIS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV96656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02957-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="58346" checksum="156CD7F4E60E410B" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GV28</accession>
    <name>A0A180GV28_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_26277</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000018">
        <property type="protein sequence ID" value="OAV96610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GV28"/>
    <dbReference type="EnsemblFungi" id="PTTG_26277-t43_2">
        <property type="protein sequence ID" value="PTTG_26277-t43_2-p1"/>
        <property type="gene ID" value="PTTG_26277"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_26277"/>
    <dbReference type="OrthoDB" id="2506830at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV96610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_26277-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="170" mass="19157" checksum="C935BBB2C567DF25" modified="2016-09-07" version="1">MKARLESSVIFLSFTILYLSSISEAYLITTTISQLDQLSSSNSKQDVNNFHISTPKLLHEFIFPSGQVDVDAVLALISARLSYIPFFNKVQTDPIGCDCHSSSQHTIKSTNWSARNNPTVRKLRQSLPSLEQHLSPKFTPKKGHSIKKEKCFEYTLQNAVVSIATFYPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GVS4</accession>
    <name>A0A180GVS4_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Diphthamide biosynthesis protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_02117</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000022">
        <property type="protein sequence ID" value="OAV96093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GVS4"/>
    <dbReference type="EnsemblFungi" id="PTTG_02117-t43_2">
        <property type="protein sequence ID" value="PTTG_02117-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02117"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02117"/>
    <dbReference type="OrthoDB" id="445556at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052276">
        <property type="entry name" value="Diphthamide-biosynth_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44240">
        <property type="entry name" value="DNAJ DOMAIN (PROKARYOTIC HEAT SHOCK PROTEIN)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44240:SF10">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
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        <property type="entry name" value="DNAJ_2"/>
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    <proteinExistence type="predicted"/>
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        <location>
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            <end position="141"/>
        </location>
    </feature>
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        <location>
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            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV96093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02117-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="171" mass="19047" checksum="B930D750EDD3585B" modified="2016-09-07" version="1">MQSVTDAVALRVGVPTPHPEGRTPRLLGGRALGLFTSQIGKAIQMVFSSASSEDERTDDNYYKILQVSSDASPSELRTAYLRLIKLHHPDKLKKQSHHPSSGPQASHSSQPNADHRFAQDLIHAYSTLIDPSRRTEYDLKRLQSPAGHNTHHRESMRRSCPKCSIFLNSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GW21</accession>
    <name>A0A180GW21_PUCT1</name>
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        <submittedName>
            <fullName evidence="3">Vps53_N domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_00063</name>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000016">
        <property type="protein sequence ID" value="OAV96960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GW21"/>
    <dbReference type="EnsemblFungi" id="PTTG_00063-t43_2">
        <property type="protein sequence ID" value="PTTG_00063-t43_2-p1"/>
        <property type="gene ID" value="PTTG_00063"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00063"/>
    <dbReference type="OrthoDB" id="10261632at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Vps53"/>
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    <dbReference type="InterPro" id="IPR007234">
        <property type="entry name" value="Vps53_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 53 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VACUOLAR SORTING PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04100">
        <property type="entry name" value="Vps53_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps53 N-terminal" evidence="1">
        <location>
            <begin position="12"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV96960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00063-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="638" mass="70847" checksum="69A6E74DDC88C1D2" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GWS8</accession>
    <name>A0A180GWS8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_12069</name>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000017">
        <property type="protein sequence ID" value="OAV96819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0A180GWS8"/>
    <dbReference type="EnsemblFungi" id="PTTG_12069-t43_1">
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        <property type="gene ID" value="PTTG_12069"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12069"/>
    <dbReference type="OrthoDB" id="2501122at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
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    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV96819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12069-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="643" mass="72423" checksum="15278EBE00587639" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GWV8</accession>
    <name>A0A180GWV8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_05904</name>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000014">
        <property type="protein sequence ID" value="OAV97275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GWV8"/>
    <dbReference type="EnsemblFungi" id="PTTG_05904-t43_2">
        <property type="protein sequence ID" value="PTTG_05904-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05904"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05904"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV97275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05904-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="352" mass="38348" checksum="199AE16D92DE985F" modified="2016-09-07" version="1">MAERAMKAEEDGDMAMANRLYNIGAGLGRAVLRANPATAVPSGAGTANSPLIIEGLRVTVPITGNNQITKEIKVVQPLDKTKPSETASGTGGLICDNSAVPTSVNDKAIMHWSEKKPKAEAAGTDVFCYLGYPYPSEYCQTYNKWSVNHQGFYAALRKIPTTAHTTFAGWLVMHKKHCDTIIIRDGYMTGLRYDIQIRANAFAHRVTLGDGRRSVANISVYREDVVRKAYGKATKLDKVNFPNKLYSAGGRRYGWDTATASQKLKATTEVTLFPYTSANPRAVKPKESSKDSNEGLLQTKQGGMRKAAREARSKTREVTKVTGTTPGTVTEREAKTAVGTEAEEGTFSRQTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GX61</accession>
    <name>A0A180GX61_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Vps53_N domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PTTG_00063</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VPS53 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000016">
        <property type="protein sequence ID" value="OAV96959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GX61"/>
    <dbReference type="EnsemblFungi" id="PTTG_00063-t43_1">
        <property type="protein sequence ID" value="PTTG_00063-t43_1-p1"/>
        <property type="gene ID" value="PTTG_00063"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00063"/>
    <dbReference type="OrthoDB" id="10261632at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.110:FF:000004">
        <property type="entry name" value="Vacuolar-sorting protein 53 long isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.110">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 53, C-terminus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039766">
        <property type="entry name" value="Vps53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031745">
        <property type="entry name" value="Vps53_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038260">
        <property type="entry name" value="Vps53_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007234">
        <property type="entry name" value="Vps53_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 53 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16854">
        <property type="entry name" value="VPS53_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04100">
        <property type="entry name" value="Vps53_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps53 N-terminal" evidence="8">
        <location>
            <begin position="50"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Vps53 C-terminal" evidence="9">
        <location>
            <begin position="629"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="740"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="778"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="750"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV96959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00063-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="833" mass="91508" checksum="B75F1E228CDA55E0" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GXT4</accession>
    <name>A0A180GXT4_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TATA-binding protein-associated factor mot1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Modifier of transcription 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PTTG_05791</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15">1-1 BBBD Race 1</strain>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
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            <strain evidence="16">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="17">Isolate 1-1 / race 1 (BBBD)</strain>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">isolate 1-1 / race 1 (BBBD)</strain>
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                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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                <person name="Khalil H.B."/>
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                <person name="Joly D."/>
                <person name="Linning R."/>
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                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV96973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11648-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="546" mass="60885" checksum="77B8B6B1231D7BE7" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GYX8</accession>
    <name>A0A180GYX8_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lon protease homolog, mitochondrial</fullName>
            <ecNumber evidence="10">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PIM1</name>
        <name evidence="15" type="ORF">PTTG_25884</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="16">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homohexamer or homoheptamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="10"/>
    <dbReference type="EMBL" id="ADAS02000010">
        <property type="protein sequence ID" value="OAV97940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="ADAS02000010">
        <property type="protein sequence ID" value="OAV97941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GYX8"/>
    <dbReference type="EnsemblFungi" id="PTTG_25884-t43_1">
        <property type="protein sequence ID" value="PTTG_25884-t43_1-p1"/>
        <property type="gene ID" value="PTTG_25884"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_25884-t43_3">
        <property type="protein sequence ID" value="PTTG_25884-t43_3-p1"/>
        <property type="gene ID" value="PTTG_25884"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_25884"/>
    <dbReference type="OrthoDB" id="2411602at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070407">
        <property type="term" value="P:oxidation-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000019">
        <property type="entry name" value="Lon protease homolog 2, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000155">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5270:FF:000001">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1480:FF:000003">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03120">
        <property type="entry name" value="lonm_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027503">
        <property type="entry name" value="Lonm_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718">
        <property type="entry name" value="LON PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718:SF2">
        <property type="entry name" value="LON PROTEASE HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 10 11" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="14">
        <location>
            <begin position="178"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="13">
        <location>
            <begin position="992"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="24"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="111"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="304"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="893"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="117"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="895"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="947"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="1088"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="1131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="654"/>
            <end position="661"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OAV97940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_25884-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="1201" mass="131091" checksum="5855E626271802F7" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GYZ6</accession>
    <name>A0A180GYZ6_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
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        <name evidence="16" type="ORF">PTTG_09819</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="18">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="ADAS02000012">
        <property type="protein sequence ID" value="OAV97579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_09819-t43_2">
        <property type="protein sequence ID" value="PTTG_09819-t43_2-p1"/>
        <property type="gene ID" value="PTTG_09819"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09819"/>
    <dbReference type="OrthoDB" id="5600252at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000819">
        <property type="entry name" value="ATP dependent RNA helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000500">
        <property type="entry name" value="ATP-dependent RNA helicase DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000002">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056328">
        <property type="entry name" value="DSRM_DHX29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF145">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX57-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24385">
        <property type="entry name" value="DSRM_DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="527"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="804"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="253"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1350"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1366"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OAV97579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09819-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="1429" mass="158372" checksum="1BE9CA03878D62F7" modified="2016-09-07" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GZ78</accession>
    <name>A0A180GZ78_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_25775</name>
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    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000009">
        <property type="protein sequence ID" value="OAV98040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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                <person name="Szabo L."/>
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                <person name="Zeng Q."/>
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                <person name="Zeng Q."/>
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                <person name="Szabo L."/>
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                <person name="Zeng Q."/>
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                <person name="Zeng Q."/>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
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        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="OAV97792.1"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H0E9</accession>
    <name>A0A180H0E9_PUCT1</name>
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        <submittedName>
            <fullName evidence="9">Fip1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_00274</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FIP1 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H0E9"/>
    <dbReference type="EnsemblFungi" id="PTTG_00274-t43_2">
        <property type="protein sequence ID" value="PTTG_00274-t43_2-p1"/>
        <property type="gene ID" value="PTTG_00274"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00274"/>
    <dbReference type="OrthoDB" id="2507772at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Fip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051187">
        <property type="entry name" value="Pre-mRNA_3'-end_processing_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484">
        <property type="entry name" value="FIP1-LIKE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484:SF0">
        <property type="entry name" value="PRE-MRNA 3'-END-PROCESSING FACTOR FIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05182">
        <property type="entry name" value="Fip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="329"/>
        </location>
    </feature>
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        </location>
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            <end position="672"/>
        </location>
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            <end position="997"/>
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            <end position="40"/>
        </location>
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    </feature>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF05182"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="OAV98201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00274-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="997" mass="108561" checksum="D90241DCCACD769C" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H2F8</accession>
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        <submittedName>
            <fullName evidence="5">CxC1 domain-containing protein</fullName>
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        <name evidence="4" type="ORF">PTTG_25419</name>
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        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000004">
        <property type="protein sequence ID" value="OAV98971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0A180H2F8"/>
    <dbReference type="EnsemblFungi" id="PTTG_25419-t43_2">
        <property type="protein sequence ID" value="PTTG_25419-t43_2-p1"/>
        <property type="gene ID" value="PTTG_25419"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_25419"/>
    <dbReference type="OrthoDB" id="2503722at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="3">
        <location>
            <begin position="141"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="41"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="969"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="621"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="970"/>
            <end position="981"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV98971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_25419-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="1003" mass="115506" checksum="9C21FC7E94FD6912" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H2G8</accession>
    <name>A0A180H2G8_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phospholipid:diacylglycerol acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_00014</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000004">
        <property type="protein sequence ID" value="OAV99195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H2G8"/>
    <dbReference type="EnsemblFungi" id="PTTG_00014-t43_1">
        <property type="protein sequence ID" value="PTTG_00014-t43_1-p1"/>
        <property type="gene ID" value="PTTG_00014"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00014"/>
    <dbReference type="OrthoDB" id="190846at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003386">
        <property type="entry name" value="LACT/PDAT_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02450">
        <property type="entry name" value="LCAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV99195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00014-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="575" mass="64099" checksum="C4342C16EBED26FC" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H2I9</accession>
    <name>A0A180H2I9_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DPBB_1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_02452</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000004">
        <property type="protein sequence ID" value="OAV99001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H2I9"/>
    <dbReference type="EnsemblFungi" id="PTTG_02452-t43_2">
        <property type="protein sequence ID" value="PTTG_02452-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02452"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02452"/>
    <dbReference type="OrthoDB" id="623670at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22191">
        <property type="entry name" value="DPBB_RlpA_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051477">
        <property type="entry name" value="Expansin_CellWall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836:SF27">
        <property type="entry name" value="RLPA-LIKE PROTEIN DOUBLE-PSI BETA-BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008110569" evidence="3">
        <location>
            <begin position="26"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="RlpA-like protein double-psi beta-barrel" evidence="4">
        <location>
            <begin position="186"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="152"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OAV99001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02452-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="285" mass="30498" checksum="B9095E6A1F714DCA" modified="2016-09-07" version="1" fragment="single">MHTTRMSTLGCLVFTFSISARYGHAADLQNFIPASPSPMTSPTTEFPQQLDATSFHAAIIRKARRSDSLSVATNNTSSLVVKPRWKKCSRGIKSRYCRSYHRNLKDRKSALKSDSSLKVGNVKAVSIPIPDVAAPVPVPKLEITSTPRVEVPSTPKVEVPSTPKVEVPSTPLTPKLEVPPIGLSTNSGDATYYGTGMGACGIVSNDNSMIAAASHLLFDSFPGATANPNMNPICGRKVKATYQGKSVVVEVVDRCTGCALHDLDFSPAAFAQIGPMERGRLHGMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H2J3</accession>
    <name>A0A180H2J3_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_06846</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000004">
        <property type="protein sequence ID" value="OAV99225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H2J3"/>
    <dbReference type="EnsemblFungi" id="PTTG_06846-t43_2">
        <property type="protein sequence ID" value="PTTG_06846-t43_2-p1"/>
        <property type="gene ID" value="PTTG_06846"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06846"/>
    <dbReference type="OrthoDB" id="411211at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005373">
        <property type="entry name" value="PHAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039156">
        <property type="entry name" value="PHAF1/BROMI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465:SF2">
        <property type="entry name" value="PHAGOSOME ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465">
        <property type="entry name" value="UPF0183 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03676">
        <property type="entry name" value="PHAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV99225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06846-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="368" mass="40881" checksum="591C5E535E9E2BA9" modified="2016-09-07" version="1">MKIGACPPLGDWVHYQNQPISQAIGPNEPGVTLRVIHRLFGPTYPPAPHTAHPNEMVVSYPGVTFSFRYEVLSRIILSVQPAGEGCKKDEGETVQLTEAYFKPKMPVHMSSIDGDVRSAKINLGRMELEESTSITFSFHSSSPNREIPDVNVTIGSSNSEDILCEFGAPLRTFWKEDDRMNIHTHLRSNGRSAGHPEDPNPYFMSYFNIGVDFLIDPITNLVLKVVLHSNIPGEVLFARYSRCPWSLRSMNQDSDVASSTDKAVHINQRIRSNFTHKEPENYASGGVVDSGQLCESFNSRAFDGSPALRAPEASSPISNIIEPAMLLDRTADVIDDGLVLRRPTRLHGHPGIVLEVTEDDDVETVWLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H3F7</accession>
    <name>A0A180H3F7_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_06158</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000004">
        <property type="protein sequence ID" value="OAV99158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H3F7"/>
    <dbReference type="EnsemblFungi" id="PTTG_06158-t43_2">
        <property type="protein sequence ID" value="PTTG_06158-t43_2-p1"/>
        <property type="gene ID" value="PTTG_06158"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06158"/>
    <dbReference type="OrthoDB" id="2506772at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018588">
        <property type="entry name" value="Dihaem_cytochrome-c"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09626">
        <property type="entry name" value="DHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV99158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06158-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="372" mass="43210" checksum="66032C6F91E3CA76" modified="2016-09-07" version="1">MKPKAPEPQLPHPISSLTRSIVEFAKFMMGTTGRDYQLPDPPTTEELKYFEFWNISIQNNQDTQATQRPLPPQIAIGDDVIQDKVENDLPGIDYKPAPIPPNYEISKHVKHECDAQFCLRGLPRITFQWNKPSLKTSPWNSASAQILADQWHSWYLEKRHYSRSTKLKVDARAVIARWLKTAAGKQRRITKEELSLREFRLDHFKEQHKQARRQIGLSRRMTAHACFPKHKNFFSKLYEMEVVSDYDYEADDDDDEAPPTRILPYWRSKVLGDLSHELDLAAIQLASPEEKPTISKLLQRRETRDATEFEASLEKIPLEAFTECFSDEFLQDTSVLERRQLQINETTQPYSLSQALADLQKLTCKENARPID</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H3H1</accession>
    <name>A0A180H3H1_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PTTG_04584</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG2 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000003">
        <property type="protein sequence ID" value="OAV99334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H3H1"/>
    <dbReference type="EnsemblFungi" id="PTTG_04584-t43_2">
        <property type="protein sequence ID" value="PTTG_04584-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04584"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04584"/>
    <dbReference type="OrthoDB" id="332281at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009316">
        <property type="entry name" value="COG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024603">
        <property type="entry name" value="COG_complex_COG2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024602">
        <property type="entry name" value="COG_su2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12961">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12961:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12022">
        <property type="entry name" value="COG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06148">
        <property type="entry name" value="COG2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 2 N-terminal" evidence="10">
        <location>
            <begin position="48"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="COG complex component COG2 C-terminal" evidence="11">
        <location>
            <begin position="511"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="189"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="411"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="623"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="411"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OAV99334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04584-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="680" mass="75680" checksum="EDF370A0A2DC6BA5" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H3H5</accession>
    <name>A0A180H3H5_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methylthioribulose-1-phosphate dehydratase</fullName>
            <shortName evidence="7">MTRu-1-P dehydratase</shortName>
            <ecNumber evidence="7">4.2.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MDE1</name>
        <name evidence="10" type="ORF">PTTG_25214</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-(methylsulfanyl)-D-ribulose 1-phosphate = 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:15549"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="ChEBI" id="CHEBI:58828"/>
            <dbReference type="EC" id="4.2.1.109"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 2/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the aldolase class II family. MtnB subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.109" evidence="7"/>
    <dbReference type="EMBL" id="ADAS02000002">
        <property type="protein sequence ID" value="OAV99567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H3H5"/>
    <dbReference type="EnsemblFungi" id="PTTG_25214-t43_1">
        <property type="protein sequence ID" value="PTTG_25214-t43_1-p1"/>
        <property type="gene ID" value="PTTG_25214"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_25214"/>
    <dbReference type="OrthoDB" id="191080at2759"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00875"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046570">
        <property type="term" value="F:methylthioribulose 1-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000003">
        <property type="entry name" value="Methylthioribulose-1-phosphate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03116">
        <property type="entry name" value="Salvage_MtnB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017714">
        <property type="entry name" value="MethylthioRu-1-P_deHdtase_MtnB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027514">
        <property type="entry name" value="Salvage_MtnB_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03328">
        <property type="entry name" value="salvage_mtnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10640">
        <property type="entry name" value="METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10640:SF7">
        <property type="entry name" value="METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="9">
        <location>
            <begin position="150"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV99567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_25214-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="371" mass="41652" checksum="A2547264D190CEB8" modified="2016-09-07" version="1">MALEPTEIQMGDIEVLDNKDLRLDGSTSFQTPSTPVTTYTSPSGSNKRSWVWAHFIEIDNGTSVKCTVVKRGGDPCGRVLKKDKTGSTKSMHEHLMALHKLGDPQKRARTEASIDKYLAKNAKSIPHVREMCESLASQGKSLEDPMHPANLICELCRNFYQLGWVTGTGGGISIRQKDHVFVAPSGVQKERMKPCDIFILDLFTREQLRRPSTPLKQSACTPLFFNAYEHRSAGACIHTHSQHAVMATLLWPGQEFRCSHLEMIKGMRVKGTEKSMSYLDTLIVPIIENTPHEEDLREDMEKAMLRYPDAPAVLVRRHGTYSWGKDWEQAKCQAECLDYLLEMAVKMKLAGLPTEVQTDRPAIEPSLAAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H3K1</accession>
    <name>A0A180H3K1_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AP complex subunit sigma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_25363</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000003">
        <property type="protein sequence ID" value="OAV99391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H3K1"/>
    <dbReference type="EnsemblFungi" id="PTTG_25363-t43_2">
        <property type="protein sequence ID" value="PTTG_25363-t43_2-p1"/>
        <property type="gene ID" value="PTTG_25363"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_25363"/>
    <dbReference type="OrthoDB" id="10261046at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="6">
        <location>
            <begin position="122"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV99391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_25363-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="178" mass="20079" checksum="BCF5A9891CA7FA44" modified="2016-09-07" version="1">MIHAVLVFNNDGKPRLSKFYSQPGSSPALTSKERRTKDCLISSSSPSNLKYEFLKTIYNLTNHHQNTKAGGVSSSKTNFINLDHSVLALLNKPLVHKNALPNAGPPNLDFFQLHPEYLNLELCLVYRNYATLFFVFLIDQSESQLGILDLIQVFVESLDKCFKNWNRSSLTSNPSSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H3L7</accession>
    <name>A0A180H3L7_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Kinesin motor domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_04413</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000002">
        <property type="protein sequence ID" value="OAV99597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H3L7"/>
    <dbReference type="EnsemblFungi" id="PTTG_04413-t43_2">
        <property type="protein sequence ID" value="PTTG_04413-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04413"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04413"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF9">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="3">
        <location>
            <begin position="56"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="454"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="493"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="586"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="632"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
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                <person name="Heilman E."/>
                <person name="Heiman D."/>
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                <person name="Howarth C."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
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                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
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                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
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                <person name="Adiconis X."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
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                <person name="Bruce M."/>
                <person name="Wang M."/>
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                <person name="Szabo L.J."/>
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                <person name="Feldgarden M."/>
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                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
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                <person name="Nusbaum C."/>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
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    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000001">
        <property type="protein sequence ID" value="OAW00084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H6N9"/>
    <dbReference type="EnsemblFungi" id="PTTG_05934-t43_1">
        <property type="protein sequence ID" value="PTTG_05934-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05934"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05934"/>
    <dbReference type="OrthoDB" id="2506937at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="732"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="782"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="789"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAW00084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05934-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="876" mass="96636" checksum="37862296CB85C6F5" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H6Q9</accession>
    <name>A0A180H6Q9_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_01551</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000001">
        <property type="protein sequence ID" value="OAW00220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblFungi" id="PTTG_01551-t43_2">
        <property type="protein sequence ID" value="PTTG_01551-t43_2-p1"/>
        <property type="gene ID" value="PTTG_01551"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_01551"/>
    <dbReference type="OrthoDB" id="10253115at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.38.10">
        <property type="entry name" value="Luciferase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201:SF30">
        <property type="entry name" value="AMP-DEPENDENT SYNTHETASE_LIGASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="109"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="2">
        <location>
            <begin position="546"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAW00220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_01551-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H6R1</accession>
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        <submittedName>
            <fullName evidence="8 9">Arf/Sar family, other</fullName>
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        <name evidence="8" type="ORF">PTTG_25205</name>
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    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
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    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the small GTPase superfamily. Arf family.</text>
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    <dbReference type="EnsemblFungi" id="PTTG_25205-t43_2">
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        <property type="gene ID" value="PTTG_25205"/>
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    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0098852">
        <property type="term" value="C:lytic vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04159">
        <property type="entry name" value="Arl10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001120">
        <property type="entry name" value="ADP-ribosylation factor family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044154">
        <property type="entry name" value="Arl8a/8b"/>
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</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4EJI5</accession>
    <name>A0A0C4EJI5_PUCT1</name>
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        <recommendedName>
            <fullName evidence="6">Pre-mRNA-splicing factor CWC26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_00903</name>
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        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
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                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">1-1 BBBD Race 1</strain>
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    </reference>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWC26 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="PTTG_00903"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:RES complex"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CWC26_splicing_factor"/>
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        <property type="entry name" value="BUD13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BUD13 HOMOLOG"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Bud13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
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        <location>
            <begin position="256"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011069"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="OAV93292.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00903-t43_1-p1"/>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="36214" checksum="F3CBA086B9DFD3A8" modified="2015-04-01" version="1">MAEDLQAYLASKYMSGPKAEAILAARKDGSKKKSKKKVKSSETNEQLAPSYSGMILKDVDGEDEWGSGVRDEELDAAPVVENVPTFKGKSSAESKWETIKPSQRAETKEEEDDEREPEDERPQIVASSSTNVVAGGLQTAEAIRAKFASKPTVAAPVSDDEPEEETIYRDSQGRKIDTKQMRAEEKRRQARELEKKMKKMEWGKGLVQREDRKTNAKELSKIASQPFARTIDDKELNDELKGKQRWNDPAARFLTKSSDSSSKSTKPKYNGPPPPPNRYGIPPGFRWDGVDRSNGFEKKLFQTGNDQKRLRAEAYNYSVDEM</sequence>
</entry>
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    <accession>A0A0C4ERZ9</accession>
    <name>A0A0C4ERZ9_PUCT1</name>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">PTTG_03569</name>
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    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
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    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02001058">
        <property type="protein sequence ID" value="OAV86508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4ERZ9"/>
    <dbReference type="EnsemblFungi" id="PTTG_03569-t43_2">
        <property type="protein sequence ID" value="PTTG_03569-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03569"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03569"/>
    <dbReference type="OMA" id="HIMTVEA"/>
    <dbReference type="OrthoDB" id="2496966at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV86508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03569-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="394" mass="44565" checksum="84E167543E58CCAD" modified="2015-04-01" version="1">MSRFGLDLFARENPPPGYFTSSEYQRIHLSGEPTKDLPPQIIVCNTGFKLLCPKNGNQCRAQWTPVNSSMAHGNFDVLITARPTPLAEFQEKIASACDEQFDNAGDLIRNALKKGSPRIVWKLLMHAKVAPEFAKSKNHTINNAPSFDRWMDAIIKLGKDRTKVLLLIEMINPEINLKKSKAVVEVKNHIMTVEAAQQASLTRPALDHPANVITADRFSPINIQMNLIYSTHPPNSKYHKKFPVFIHATDLNQFIPLTTAIVQQWATDLVNVVAGISIYSPPRGLKYEVLSLKRRKLVHTSEPCEQSQSVDSADSEVITPDPQVLLDYIEFIKIRPSKRDKVLAILNEKDITHPKFFKSKQITNKKMLQWGLSNGIIYQLKENVSKFEKHLALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4F129</accession>
    <name>A0A0C4F129_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_06799</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000083">
        <property type="protein sequence ID" value="OAV91289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4F129"/>
    <dbReference type="EnsemblFungi" id="PTTG_06799-t43_2">
        <property type="protein sequence ID" value="PTTG_06799-t43_2-p1"/>
        <property type="gene ID" value="PTTG_06799"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06799"/>
    <dbReference type="OMA" id="IELIKCH"/>
    <dbReference type="OrthoDB" id="2529772at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF27">
        <property type="entry name" value="GH27708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="115"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV91289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06799-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="53089" checksum="D1E52F7E78153B86" modified="2015-04-01" version="1">MALLTDLPAELLRKILEHFIQLHQANLDELKRPWNRCFFYPGKLDIRLRASSNLRSKETYHSNFQLLNETGLVSFVFLKLSPGRRQALFAHVTLIDQWQAHLFYQALTSPSTLQDQLELPDDSPDTHGKRSRTQDIQARSTPSDQHPPTSLACQVRSLQFKFVGPCSLGRGGGSLVCQILRACPLVENITIATGFMTCCQEPLLDALASRPFIKTFVLLESFGKNTSFLPWTAEQLLGRLFTKWESLQTIELIKCHVNHRPLWEPGGYDSFLPALNCALRTIVLNCPCLKEKDLAMILNGSRASLRNLQICHPTPALHRPGLYRILTECISPALESLTLEVNDRWGKMIFPQADYPDLNPGLLDHVFKTSSTLSNLKELSFAGDLTGEDFLDLLPQSIVKLTWNRCGISFEAFSEALTSGTVNSLELPLPLQLDPPSGAETRRPRWLPELKCCSIYNFDKCMSPFHFFCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G0M2</accession>
    <name>A0A180G0M2_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase S9 prolyl oligopeptidase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_09850</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02001565">
        <property type="protein sequence ID" value="OAV86154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G0M2"/>
    <dbReference type="EnsemblFungi" id="PTTG_09850-t43_2">
        <property type="protein sequence ID" value="PTTG_09850-t43_2-p1"/>
        <property type="gene ID" value="PTTG_09850"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09850"/>
    <dbReference type="OrthoDB" id="9988524at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="1">
        <location>
            <begin position="178"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV86154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09850-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="276" mass="31022" checksum="390E292AF6EAD4A3" modified="2016-09-07" version="1" fragment="single">LHPAPLLPGLVILLHGRLHNSTRGPCSELFSKISYSTLTYDARGMGESSGTTGWCNIDQIISHIRSTFGPPLNQVIAIIGHSKGAGVGFRWASLKDLHQEISQPHLYDQIDRPPLLVSLSARYDTSDVGLSRFNQEQQAKLENEGKFVWLSYRAGRNFCERRDYIVTLEDIEKATKVSLECVKNMRENAQVLLFHGRADGTVAVQHAEKLGRQLRDTNPSTSVDLVLVEGAKHNWDQEGEIEMLVDWINPWIKRRAGRDSTRIPADKIVDHLGPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G1P6</accession>
    <name>A0A180G1P6_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vacuolar protein sorting-associated protein 52</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_03043</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS52 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02001223">
        <property type="protein sequence ID" value="OAV86369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G1P6"/>
    <dbReference type="EnsemblFungi" id="PTTG_03043-t43_1">
        <property type="protein sequence ID" value="PTTG_03043-t43_1-p1"/>
        <property type="gene ID" value="PTTG_03043"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03043"/>
    <dbReference type="OrthoDB" id="19482at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007258">
        <property type="entry name" value="Vps52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048361">
        <property type="entry name" value="Vps52_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048319">
        <property type="entry name" value="Vps52_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14190">
        <property type="entry name" value="SUPPRESSOR OF ACTIN MUTATIONS 2/VACUOLAR PROTEIN SORTING 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14190:SF7">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20655">
        <property type="entry name" value="Vps52_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04129">
        <property type="entry name" value="Vps52_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps52 coiled-coil" evidence="6">
        <location>
            <begin position="23"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Vps52 C-terminal" evidence="7">
        <location>
            <begin position="367"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OAV86369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03043-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="563" mass="62770" checksum="302F03CA3CFC763D" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G204</accession>
    <name>A0A180G204_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BAG domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_00822</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000962">
        <property type="protein sequence ID" value="OAV86608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G204"/>
    <dbReference type="EnsemblFungi" id="PTTG_00822-t43_1">
        <property type="protein sequence ID" value="PTTG_00822-t43_1-p1"/>
        <property type="gene ID" value="PTTG_00822"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00822"/>
    <dbReference type="OrthoDB" id="2500685at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040400">
        <property type="entry name" value="BAG5/6/7/8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33322">
        <property type="entry name" value="BAG DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33322:SF4">
        <property type="entry name" value="BAG DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAG" evidence="2">
        <location>
            <begin position="188"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV86608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00822-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="281" mass="31388" checksum="5F79C39EA3C12EC6" modified="2016-09-07" version="1">MRQLGQSVPEEEMKVEVTLPDGRQCVVPISWAKKMQTKYPGILTNSMIRAHRTPSPAPAQLDQPLASESDHDSDFYVLDPTQDPSIPVLATSSPPPSAARRRTPSPQPPKHSPEEQDAAARLIQQQFRAHQSLKHLAELELTFDKLKSEFTYPALFDLKFVASPEQSDSPTPSSTKLAFNHPVNRSVQAFEEGLTQLQIKADAILSRGNPKVKSWRKRLIKAVEAQLDQLDRFKTEAWSAQHLAHLESATKNQTDDGCEDVEMTDEIMNTDVESVSSDRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G446</accession>
    <name>A0A180G446_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="11">GATA-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PTTG_11272</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
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                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
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                <person name="Adiconis X."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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                <person name="Zeng Q."/>
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                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
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                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
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                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
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                <person name="Thomson T."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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                <person name="Khalil H.B."/>
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                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
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                <person name="Heiman D."/>
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                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">1-1 BBBD Race 1</strain>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
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    <reference evidence="10" key="4">
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
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                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
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                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000281">
        <property type="protein sequence ID" value="OAV87791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G550"/>
    <dbReference type="EnsemblFungi" id="PTTG_29277-t43_3">
        <property type="protein sequence ID" value="PTTG_29277-t43_3-p1"/>
        <property type="gene ID" value="PTTG_29277"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_29277"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF8">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE GSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV87791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_29277-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="148" mass="16862" checksum="133DC802F05200DF" modified="2016-09-07" version="1">MANTRTPKPLRLITMNTPKGQEVQIVVEELRLCYEVNVVHMVVDVRTGMQKQPGFLRLNPNGKIPVLIDNNHDAINTIDRPFCVMESGAICRYLCSRFDTKRVFYFSDPCELSEMEQWVAWGLSTLNPATDECIKSLPIKEMIVLSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G551</accession>
    <name>A0A180G551_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_11572</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000277">
        <property type="protein sequence ID" value="OAV87807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G551"/>
    <dbReference type="EnsemblFungi" id="PTTG_11572-t43_1">
        <property type="protein sequence ID" value="PTTG_11572-t43_1-p1"/>
        <property type="gene ID" value="PTTG_11572"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_11572"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000016">
        <property type="entry name" value="Polyubiquitin 3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="153"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="229"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="305"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="381"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV87807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11572-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="457" mass="51130" checksum="A5AF346A29F15F43" modified="2016-09-07" version="1">MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G556</accession>
    <name>A0A180G556_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_08870</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
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                <person name="Szabo L."/>
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    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08421-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="446" mass="48048" checksum="697D48159A41161C" modified="2016-09-07" version="1">MPGPMDIDEPEGGGRGCPSSTNELDETKKLMGGEWNCSYLLPIPILTIEGCEANPGAKERIENEFRVSVTFKQTMPHYKGFGNGLCRILAQDSLAIYRSLNLVVALVRLSAPSRYCFWTHWLFPQEIIWNSHINHQKTNNPSILNYKPPLPAGGFCNEQDPSKLAAAGNPGSSADQGHKDPQSFFRMEDEVPGAPSTEPAAPIETDEPYRWPKEPSPTGQPGGVISSSTPLDTPYSPEPGPPAGSPIDTPPVLDHRPSPHSPTDTPPVLESPSAAHFPPGADNYLVTTTPCSSLEERARAPADSRRVFSIQPTTPASDELGPDHAELTPRAAHKPDPTHLALDITASEPVLPPDHPDSQQSFTWLNGSLELAQDRPPPQAPPHPSPPTPNQDATESQQTVNTSQSSHENPTTESSGMTSSVLELPLTIKEVISIQEGVLRGSGDHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G5X8</accession>
    <name>A0A180G5X8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF2235 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_06865</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000216">
        <property type="protein sequence ID" value="OAV88087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G5X8"/>
    <dbReference type="EnsemblFungi" id="PTTG_06865-t43_1">
        <property type="protein sequence ID" value="PTTG_06865-t43_1-p1"/>
        <property type="gene ID" value="PTTG_06865"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06865"/>
    <dbReference type="OrthoDB" id="3057168at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018712">
        <property type="entry name" value="Tle1-like_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33840:SF1">
        <property type="entry name" value="TLE1 PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09994">
        <property type="entry name" value="T6SS_Tle1-like_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T6SS Phospholipase effector Tle1-like catalytic" evidence="1">
        <location>
            <begin position="4"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV88087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06865-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="451" mass="51579" checksum="DA517AC0E4342B6F" modified="2016-09-07" version="1">MTEVESNVSKLARCVCPEDRRTSPPTPQIVFYQPGQATFSQETSGTDVVNKIREAYAFLVQHYVSGDELLLFGFSRGAFIARTLAQMVIEIGILNMAGMSDFYEVFAAYQLCVKGGTEGAASARNFLDHYRHSGQKELRPVTEGTLKCVGVWDTVGVFGLPDHFADKYRLLGFKDRKLSAQIKYAFHAIALCEARKDLQPTRWESEPSPQDCDHPGQVLKQVWFPCSHVEAGGRIEEYDDVTYMSLIWMVANMINYNLLAMNEEYLHGLLSVFWTRVQRGFGLYDPEGPRRYCLSSTARQFPTRWNATTKETIHRSVFSSQELIPGLEKVTKEGDPTLIEELLPFEMEIRNHLDFLMKEWAIHEGHRVGANEFDDWPGQDSWYREQWYKNFKETELGDKPRTHALFLDLLTESSWPVDTKHVKEEMISSRRGRGHTAVEDAALRIIGTPID</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G657</accession>
    <name>A0A180G657_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Elongator complex protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PTTG_07524</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="15">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ELP1/IKA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000204">
        <property type="protein sequence ID" value="OAV88161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_07524-t43_1">
        <property type="protein sequence ID" value="PTTG_07524-t43_1-p1"/>
        <property type="gene ID" value="PTTG_07524"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_07524"/>
    <dbReference type="OrthoDB" id="40048at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056167">
        <property type="entry name" value="A-sol_ELP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056164">
        <property type="entry name" value="Beta-prop_ELP1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056165">
        <property type="entry name" value="Beta-prop_ELP1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006849">
        <property type="entry name" value="Elp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056169">
        <property type="entry name" value="HB_ELP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056166">
        <property type="entry name" value="TPR_ELP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12747">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12747:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23925">
        <property type="entry name" value="A-sol_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04762">
        <property type="entry name" value="Beta-prop_ELP1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23797">
        <property type="entry name" value="Beta-prop_ELP1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23936">
        <property type="entry name" value="HB_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23878">
        <property type="entry name" value="TPR_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017233">
        <property type="entry name" value="IKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="ELP1 first N-terminal beta-propeller" evidence="9">
        <location>
            <begin position="62"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 N-terminal second beta-propeller" evidence="10">
        <location>
            <begin position="431"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 alpha-solenoid" evidence="12">
        <location>
            <begin position="780"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 TPR" evidence="11">
        <location>
            <begin position="993"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 three-helical bundle" evidence="13">
        <location>
            <begin position="1168"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1249"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1363"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1177"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1253"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1270"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1374"/>
            <end position="1383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OAV88161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_07524-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="1406" mass="156454" checksum="1A471416B6154E48" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G6W2</accession>
    <name>A0A180G6W2_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="17">CFEM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PTTG_04062</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="17">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RBT5 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000177">
        <property type="protein sequence ID" value="OAV88435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G6W2"/>
    <dbReference type="EnsemblFungi" id="PTTG_04062-t43_2">
        <property type="protein sequence ID" value="PTTG_04062-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04062"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04062"/>
    <dbReference type="OrthoDB" id="2507492at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051735">
        <property type="entry name" value="CFEM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008427">
        <property type="entry name" value="Extracellular_membr_CFEM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37928">
        <property type="entry name" value="CFEM DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G14090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37928:SF2">
        <property type="entry name" value="GPI ANCHORED CFEM DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G10580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05730">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00747">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52012">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008109618" evidence="14">
        <location>
            <begin position="23"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="CFEM" evidence="15">
        <location>
            <begin position="6"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS52012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OAV88435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04062-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="101" mass="10475" checksum="9E9C34B9DE628B94" modified="2016-09-07" version="1">MLPNTLRFTTLLGYSLLVSTLATIEQDFSSLPQCSVQCLTTSLGSSGSPCAPTDFACLCKNKKFLAASDSCNKSSCSGKDAKAAKTWGEKACQGVGVKIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G7Y6</accession>
    <name>A0A180G7Y6_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_06557</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000152">
        <property type="protein sequence ID" value="OAV88817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G7Y6"/>
    <dbReference type="EnsemblFungi" id="PTTG_06557-t43_2">
        <property type="protein sequence ID" value="PTTG_06557-t43_2-p1"/>
        <property type="gene ID" value="PTTG_06557"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06557"/>
    <dbReference type="OrthoDB" id="74240at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13675">
        <property type="entry name" value="LYR MOTIF-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13675:SF0">
        <property type="entry name" value="LYR MOTIF-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="3">
        <location>
            <begin position="29"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV88817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06557-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="106" mass="12369" checksum="F8E19EB76EE7D579" modified="2016-09-07" version="1">MISQTIKFQKSRIPSDGLSLGHFVFQTKIRSLYRNILRETRYLGTNQIRGETISWIRYEFMKPIPGKLDKHNNQDFLAQINRQLKQIKNSSMLIGGGFEKLRGGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GBP5</accession>
    <name>A0A180GBP5_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_05235</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000109">
        <property type="protein sequence ID" value="OAV90105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GBP5"/>
    <dbReference type="EnsemblFungi" id="PTTG_05235-t43_1">
        <property type="protein sequence ID" value="PTTG_05235-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05235"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05235"/>
    <dbReference type="OrthoDB" id="2505192at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV90105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05235-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="421" mass="45380" checksum="F93665434BEC2AC5" modified="2016-09-07" version="1">MLNPGQTRLPLASLDFGDRDLTDGFTDVRDGTHSSISGFGHPRGSTPISVLSSRRSTTSTALSGESPESRRVLPNMSALLDFLDNDNTNPFPQTSSSNRKQVQAASHRSSRSPPQPSPALHPHPPRNSLTPRPMPADADLTSNFLHTYDRTNPFRGALPASSTPFTNHRDDFSEMSSPLNKRHNVPSGLPANISPIQFAHQMAAAPTSNSSAHKHASENRPRHHSPRSERPVEALPLETPARLPAKAPSAADRMAAAVMEGAPESHWTFLMERGSSENTAGIRRSKVVEELLQPMRPSISSPDKQITQIPEATGSIKDSDLPQSLLDETRRPSDLPPLTTASEPIMGRSRLTRSVGYSSSPNLIRPTPQRPPLAAADVVPNSAKPTLATEPAEVLDVRVNQLVALAHSIGPVGESQFGIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GC02</accession>
    <name>A0A180GC02_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Csf1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_05662</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000115">
        <property type="protein sequence ID" value="OAV89882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_05662-t43_1">
        <property type="protein sequence ID" value="PTTG_05662-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05662"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05662"/>
    <dbReference type="OrthoDB" id="10051416at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006113">
        <property type="term" value="P:fermentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029636">
        <property type="entry name" value="Csf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048636">
        <property type="entry name" value="Csf1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32085">
        <property type="entry name" value="PROTEIN CSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32085:SF3">
        <property type="entry name" value="PROTEIN CSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21678">
        <property type="entry name" value="Csf1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Csf1 N-terminal" evidence="3">
        <location>
            <begin position="28"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1160"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2571"/>
            <end position="2590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1173"/>
            <end position="1190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV89882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05662-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="3129" mass="348595" checksum="4A2983B6A4429852" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GC23</accession>
    <name>A0A180GC23_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">OPT family small oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PTTG_28401</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
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                <person name="Zeng Q."/>
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                <person name="Berlin A."/>
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                <person name="Borenstein D."/>
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                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
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                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
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                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
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                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
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        <location>
            <begin position="893"/>
            <end position="902"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV90228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00718-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="1050" mass="112471" checksum="A3A78340CA2C128E" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GCQ4</accession>
    <name>A0A180GCQ4_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="7">N-acetyltransferase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PTTG_01943</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyltransferase family. GNAT subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000108">
        <property type="protein sequence ID" value="OAV90132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GCQ4"/>
    <dbReference type="EnsemblFungi" id="PTTG_01943-t43_1">
        <property type="protein sequence ID" value="PTTG_01943-t43_1-p1"/>
        <property type="gene ID" value="PTTG_01943"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_01943"/>
    <dbReference type="OrthoDB" id="5043642at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039135">
        <property type="entry name" value="NAT9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13256:SF16">
        <property type="entry name" value="ALPHA_BETA-TUBULIN-N-ACETYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13256">
        <property type="entry name" value="N-ACETYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="5">
        <location>
            <begin position="65"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="193"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OAV90132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_01943-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="262" mass="29529" checksum="F3D282D793E8029C" modified="2016-09-07" version="1">MSDQELRESTASEPLSLLEEYEMCERWALDQDKLTFIIFSRNSSLSIGSDPPEKHTTYSDYLGQMIGDVNLFISADESLEDSTSTPKETDGQAAPNKGELEIMIASKDHRKLGLATEALQLFLAYILLISRPLSPSQNPSQSSFPHISFFFVKISFSNTISQRLFEKLGFERTSTNKIFEEYEYRLKLSGSPVLSIDDDDDGQRNPQTDPPRQNTDQVNSNEQRDPENQVVRAYKAKWKAFCPIKMALIPCPLNDPSAASLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GCR1</accession>
    <name>A0A180GCR1_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 11">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="1 11">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 11">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PTTG_07346</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="14">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="1 11"/>
    <dbReference type="EMBL" id="ADAS02000104">
        <property type="protein sequence ID" value="OAV90319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GCR1"/>
    <dbReference type="EnsemblFungi" id="PTTG_07346-t43_1">
        <property type="protein sequence ID" value="PTTG_07346-t43_1-p1"/>
        <property type="gene ID" value="PTTG_07346"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_07346"/>
    <dbReference type="OrthoDB" id="337870at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000001">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000014">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054608">
        <property type="entry name" value="SYY-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024088">
        <property type="entry name" value="Tyr-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766:SF0">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766">
        <property type="entry name" value="TYROSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22421">
        <property type="entry name" value="SYY_C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Tyrosine--tRNA ligase SYY-like C-terminal" evidence="12">
        <location>
            <begin position="272"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OAV90319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_07346-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="342" mass="38284" checksum="EE3A9768A2A525A0" modified="2016-09-07" version="1">MIKNNLEWTKDLPLLEFLGSVGRVARVSTMLARDSVKQRLESGSGISFTEFTYQLLQAYDFAQLRSRYQCTIQLGGSDQYGNIMSGIELMAKLQSSSTTHLGTEGISGSTQQLNSDENSNHNVAFGLTVPLLTTREGEKFGKSAGNAVWLDPNLTSPVEFYQKFMSIPDSEVRKYLKMLTFVPLEELEQHFESSKHLDKPDSKRDLQKLLATEVTLLVHGEKGLRQAIQGTKFLFPTEKVEPDGCHWTREKLDVTFENSPNMIELKSTQVVGQSIGNLSVTSGLCQSKADAKRIIKSGGVRFNHRQIADFDQVVSEHDLIDSAYLLLMRGKSTNYKVVKVIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GCR8</accession>
    <name>A0A180GCR8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_28295</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000100">
        <property type="protein sequence ID" value="OAV90485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GCR8"/>
    <dbReference type="EnsemblFungi" id="PTTG_28295-t43_1">
        <property type="protein sequence ID" value="PTTG_28295-t43_1-p1"/>
        <property type="gene ID" value="PTTG_28295"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_28295"/>
    <dbReference type="OrthoDB" id="19619at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.240">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV90485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_28295-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="135" mass="15493" checksum="4271A04EDBC9A455" modified="2016-09-07" version="1">MTEFVDEAYQTIVLAHQTVTTGQFEDEQVKRFLHPLMVKFLRKKRRRLLGLYSPSHLISWMKHPPPAQESKLAVVAMRAAPFDQAGTLVQLAVRFRSRQSLEIRDERGLVVFGSHSEPQPIMEVDEDPKPDCLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GCT3</accession>
    <name>A0A180GCT3_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_12638</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000100">
        <property type="protein sequence ID" value="OAV90470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GCT3"/>
    <dbReference type="EnsemblFungi" id="PTTG_12638-t43_2">
        <property type="protein sequence ID" value="PTTG_12638-t43_2-p1"/>
        <property type="gene ID" value="PTTG_12638"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12638"/>
    <dbReference type="OrthoDB" id="3255642at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008109785" evidence="2">
        <location>
            <begin position="21"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV90470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12638-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="396" mass="43606" checksum="55BD87C5C412820F" modified="2016-09-07" version="1">MYYFHFSFWLLQLNWHLAVACEGDCAANLTKAYAEGYVRLANPVFAGLTRTIQTSLPNVTAGQVNAFIEPIRQKTYNAVHEEVKKGLQLISAQNANKSGTMNLTSDKLALSVLQVVNLVLNETLSAVSQTIATLGALDKKQPGVASSAPPVKVPAEVPPSPIKPMETPGRPFAVSTIPINAPGAPKLPKRQAHENTESREFSRSANSRENGKPQALSAHPASADFHPVPDAPVQDPCAIRLIENIICLRNLPIVSGILKRRSFASSKSPMPALLSSQHGQTFSSISKRSLGDEATLRGVPRIYILTDIQADELTSQGSPENLPDLSDEIEPKNVQSFHNPIQIPFHELDPGQEDREGRRRVESILQDAWRVFEARFKINQELLWETFRKEHLAPLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GCT6</accession>
    <name>A0A180GCT6_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_11951</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000100">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="PTTG_11951"/>
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    <dbReference type="OrthoDB" id="2499763at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="562"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="562"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="572"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="589"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="611"/>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="643"/>
            <end position="653"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="OAV90507.1"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11951-t43_2-p1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
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    </evidence>
    <sequence length="686" mass="77537" checksum="CE5557FA3EDD0BE3" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GD11</accession>
    <name>A0A180GD11_PUCT1</name>
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        <recommendedName>
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        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
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    <reference evidence="6" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblFungi" id="PTTG_07594-t43_2">
        <property type="protein sequence ID" value="PTTG_07594-t43_2-p1"/>
        <property type="gene ID" value="PTTG_07594"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_07594"/>
    <dbReference type="OrthoDB" id="6079689at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050891">
        <property type="entry name" value="TatD-type_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060:SF15">
        <property type="entry name" value="DEOXYRIBONUCLEASE TATDN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060">
        <property type="entry name" value="TATD FAMILY DEOXYRIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
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            <label>2</label>
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        <location>
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        </location>
        <ligand>
            <name>a divalent metal cation</name>
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            <label>2</label>
        </ligand>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
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            <dbReference type="EMBL" id="OAV90419.1"/>
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    </evidence>
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            <dbReference type="EnsemblFungi" id="PTTG_07594-t43_2-p1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="281" mass="31296" checksum="7713F540AC1178A5" modified="2016-09-07" version="1">MRWIPVRLASIGPEYLPLTRRALSHHSKLYSHQESRLRTMAALESPTGHPQGTTPEPQETSYTFIDIGSNLGDPVFRGEYHGKQVHADDFQQILTRARSAGVVKQILTGDCLSGSKEVRELARGNEELYATVGCHPCRANEFEASAQTDSPADVERSAKKYLEALDQLIAEDQASGHSRVVAVGECGLDYDRLSHASKETQLRYFPPQLELATKYNLPLFLHSRTSEAHVDFVRIIRAHEARYTAEELLPARKKGVVHSFTGTIEEMKELVDLGFSIGING</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GD54</accession>
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        <submittedName>
            <fullName evidence="11">Tr-type G domain-containing protein</fullName>
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    <gene>
        <name evidence="10" type="ORF">PTTG_06021</name>
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    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000097">
        <property type="protein sequence ID" value="OAV90637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GD54"/>
    <dbReference type="EnsemblFungi" id="PTTG_06021-t43_2">
        <property type="protein sequence ID" value="PTTG_06021-t43_2-p1"/>
        <property type="gene ID" value="PTTG_06021"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06021"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04098">
        <property type="entry name" value="eEF2_C_snRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04090">
        <property type="entry name" value="EF2_II_snRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01683">
        <property type="entry name" value="EF2_IV_snRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04167">
        <property type="entry name" value="Snu114p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000004">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000029">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000009">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000646">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031950">
        <property type="entry name" value="EFTUD2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044121">
        <property type="entry name" value="Snu114_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035655">
        <property type="entry name" value="U5-116kDa_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF6">
        <property type="entry name" value="116 KDA U5 SMALL NUCLEAR RIBONUCLEOPROTEIN COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16004">
        <property type="entry name" value="EFTUD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="127"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV90637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06021-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="985" mass="109236" checksum="88FB24CAA622A6B8" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GD61</accession>
    <name>A0A180GD61_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_28336</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000102">
        <property type="protein sequence ID" value="OAV90388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GD61"/>
    <dbReference type="EnsemblFungi" id="PTTG_28336-t43_1">
        <property type="protein sequence ID" value="PTTG_28336-t43_1-p1"/>
        <property type="gene ID" value="PTTG_28336"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_28336"/>
    <dbReference type="OrthoDB" id="2496263at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV90388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_28336-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="267" mass="29448" checksum="836EEFA25C2C7D69" modified="2016-09-07" version="1">MPVAVAPRKSGVAKQSDGSPDNGRKWPCDARGSVKRLPKTVALSPAGSTPPRPARAERSAPFETVATSPPSIEETMGDLRLGALESACLTLLSLFAIPLQLILSLPNPDSRPGTIPRGPLEWGHGPQLEPAPQTKHLVWDSQLDMNKGKKVEVFSVETGDVVYTISELPHDYRTTIISNGQGMELLRFQLKKTATWDHAFVYHTSSAVSYEIHPRGKATDRWYMHVQGDNPETLKYYRGHSANTGNVYLRDTKLAAFQCIYVSRFTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GE49</accession>
    <name>A0A180GE49_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FSH1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_00046</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000095">
        <property type="protein sequence ID" value="OAV90728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GE49"/>
    <dbReference type="EnsemblFungi" id="PTTG_00046-t43_1">
        <property type="protein sequence ID" value="PTTG_00046-t43_1-p1"/>
        <property type="gene ID" value="PTTG_00046"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00046"/>
    <dbReference type="OrthoDB" id="2094269at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
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    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050593">
        <property type="entry name" value="LovG"/>
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    <dbReference type="PANTHER" id="PTHR48070">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070:SF6">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV90728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00046-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="255" mass="28052" checksum="BF8191136F32AC52" modified="2016-09-07" version="1">MDTNASIFSKRLAAVRKACQGHCEMVFVDAPLVIEVPDPTNNSLSQFDSAAVANHTPETDPALIPRAWWRSKEIEDGENGTRKIYEGFDESMQFIRRVIDDQGPFDACFGFSQGAALAGIISSILEHPSLHTAFSVPPLTAQKPFKAAILVSGFQLHHPPVWHSNSDGTHTKLNTRSLHVIGKTDVIVGEDRSQSLVDVFTNPRVERHDGGHFVPSKKNWRDFFALYMQSLDQADESLVLSPGQVGNTIDGGAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GEW7</accession>
    <name>A0A180GEW7_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MADS-box domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_11893</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000092">
        <property type="protein sequence ID" value="OAV90872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GEW7"/>
    <dbReference type="EnsemblFungi" id="PTTG_11893-t43_2">
        <property type="protein sequence ID" value="PTTG_11893-t43_2-p1"/>
        <property type="gene ID" value="PTTG_11893"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_11893"/>
    <dbReference type="OrthoDB" id="2284405at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="534"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV90872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11893-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="580" mass="64612" checksum="BEF8DEAB5C2F642B" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GF52</accession>
    <name>A0A180GF52_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">GPI ethanolamine phosphate transferase 1</fullName>
            <ecNumber evidence="13">2.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PTTG_04717</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="16">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.-.-.-" evidence="13"/>
    <dbReference type="EMBL" id="ADAS02000085">
        <property type="protein sequence ID" value="OAV91204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GF52"/>
    <dbReference type="EnsemblFungi" id="PTTG_04717-t43_1">
        <property type="protein sequence ID" value="PTTG_04717-t43_1-p1"/>
        <property type="gene ID" value="PTTG_04717"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04717"/>
    <dbReference type="OrthoDB" id="2748310at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16020">
        <property type="entry name" value="GPI_EPT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000074">
        <property type="entry name" value="GPI ethanolamine phosphate transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007070">
        <property type="entry name" value="GPI_EtnP_transferase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017852">
        <property type="entry name" value="GPI_EtnP_transferase_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037671">
        <property type="entry name" value="PIGN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04987">
        <property type="entry name" value="PigN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 13" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="382"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="408"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="470"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="500"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="524"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="576"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="609"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="694"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="730"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="764"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="797"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 1 C-terminal" evidence="14">
        <location>
            <begin position="337"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OAV91204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04717-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="840" mass="95114" checksum="7B4C44387027EF37" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GF63</accession>
    <name>A0A180GF63_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aldose 1-epimerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_07761</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000090">
        <property type="protein sequence ID" value="OAV90972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GF63"/>
    <dbReference type="EnsemblFungi" id="PTTG_07761-t43_2">
        <property type="protein sequence ID" value="PTTG_07761-t43_2-p1"/>
        <property type="gene ID" value="PTTG_07761"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_07761"/>
    <dbReference type="OrthoDB" id="274691at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091:SF6">
        <property type="entry name" value="1-EPIMERASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G13240)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091">
        <property type="entry name" value="ALDOSE-1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV90972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_07761-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="48063" checksum="64CC540295D673E8" modified="2016-09-07" version="1">MGRPRRNQHSGSARVYPEYSASPAGSFDRLNHGHQEHDQDGGEDEDEESGGMWSTFPPHHHSPLLGSPSAAAFFRRLDPSSSTYRSTARGRARGFKISCRWIRMPALLLLCLLAILAFVVVPWHQSRAGLDLNQLRVSENHSDGFAVHNISAPDGSIYGSFVGLGASVQSLFVKDRHGSFRDIVLGYDNTSEYLHHPINPRMGSVVGRYANRIKNSSFAIPGHPDQIFRTTPNEHQGLNTLHGGDPGWDRRPFQVEAKNLSYISFSLVDHDGEQGFPHEVVSKIEYQLLSGGKWRIKMTAQANGPTPIMLSSHVYWNLDAYGSDESAPKHFLQLNAPQYIATDPILIPTGKLESVKNTPLDFLEPTEIGSRLNETAGLCGQDCIGYLGQCFGLRCPSALGGPSNVNVESSVGDQDVCHHRPTRRPNLLLQFNRPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GF65</accession>
    <name>A0A180GF65_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Tryptophanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PTTG_08256</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="3"/>
    <dbReference type="EMBL" id="ADAS02000083">
        <property type="protein sequence ID" value="OAV91261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GF65"/>
    <dbReference type="EnsemblFungi" id="PTTG_08256-t43_1">
        <property type="protein sequence ID" value="PTTG_08256-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08256"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08256"/>
    <dbReference type="OrthoDB" id="15808at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070183">
        <property type="term" value="P:mitochondrial tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000002">
        <property type="entry name" value="Tryptophan--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050203">
        <property type="entry name" value="Trp-tRNA_synthetase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43766">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43766:SF1">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013243968" description="tryptophan--tRNA ligase" evidence="11">
        <location>
            <begin position="16"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OAV91261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08256-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="278" mass="31024" checksum="9B54FE86A69485E9" modified="2016-09-07" version="1">MMCVLLAIGLDPAHCCLFNQDQVPAHSEMAWILNCIAPVGRLNRMTTWKSQLASSKNANSLAEVDDSMLHLGLLAYPVLQSADILLYKSTQVPVGEDQVQHLELTKVLAKALNKQTKNKLLFPLPQGIYTRQSKILNLRNPTMKMSKSNPSDLTKILITDTPEEVQRKIKSAVTDSLEGVRYDPEQRPGLSNLLDIYQSISGQELAQVERRFQDKSVVDLKAELVDLLVEHFRPFNAQFARLVNARQEVENIYLAGAQKAAAVANLTLSQLKKHIGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GF95</accession>
    <name>A0A180GF95_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PTTG_05038</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="ADAS02000098">
        <property type="protein sequence ID" value="OAV90613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GF95"/>
    <dbReference type="EnsemblFungi" id="PTTG_05038-t43_1">
        <property type="protein sequence ID" value="PTTG_05038-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05038"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05038"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14135">
        <property type="entry name" value="STKc_PRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000078">
        <property type="entry name" value="Serine/threonine-protein kinase PRP4 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044092">
        <property type="entry name" value="STKc_PRP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF103">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PRP4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="579"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="299"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="68"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="302"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="375"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="404"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="494"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="528"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="616"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OAV90613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05038-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="910" mass="102446" checksum="6340439F84BC8992" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GFV8</accession>
    <name>A0A180GFV8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_08220</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000078">
        <property type="protein sequence ID" value="OAV91555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GFV8"/>
    <dbReference type="EnsemblFungi" id="PTTG_08220-t43_1">
        <property type="protein sequence ID" value="PTTG_08220-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08220"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08220"/>
    <dbReference type="OrthoDB" id="436852at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037869">
        <property type="entry name" value="Spp1/CFP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174:SF1">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="157"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="238"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV91555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08220-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="305" mass="33993" checksum="7843F1B52AE0959C" modified="2016-09-07" version="1">MNILLQTYSSFPTASDADKQTTWKKRCARPGCHLAARVLSKYCSDWCGTERAATRLNTATTDYHRFWDSVRAATKPEGVVIIESGNPNHSSSCQANQNMLDDILLNQRTKRLEELLAKKLAVESQVQLATSRSKYLGYAISRWENMCLATARALQIEDNPSPDQSTTATTTTSKPKKGGGSGRGRGKARGDPKKSETSFLNSAISSIEAPCGFDVRLIWDDKDWAQWTSSESFSALFRSQESENNEPVQGDVNTEIGDSNPQPAMDYGIEEGVICLIMRKKCDRHLGWQKTRENDFQTEVNTLVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GFX5</accession>
    <name>A0A180GFX5_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_28190</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Regulates mitochondrial small subunit maturation by controlling 15S rRNA 5'-end processing. Localizes to the 5' precursor of the 15S rRNA in a position that is subsequently occupied by mS47 in the mature yeast mtSSU. Uses structure and sequence-specific RNA recognition, binding to a single-stranded region of the precursor and specifically recognizing bases -6 to -1. The exchange of Ccm1 for mS47 is coupled to the irreversible removal of precursor rRNA that is accompanied by conformational changes of the mitoribosomal proteins uS5m and mS26. These conformational changes signal completion of 5'-end rRNA processing through protection of the mature 5'-end of the 15S rRNA and stabilization of mS47. The removal of the 5' precursor together with the dissociation of Ccm1 may be catalyzed by the 5'-3' exoribonuclease Pet127. Involved in the specific removal of group I introns in mitochondrial encoded transcripts.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Binds to mitochondrial small subunit 15S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000093">
        <property type="protein sequence ID" value="OAV90863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GFX5"/>
    <dbReference type="EnsemblFungi" id="PTTG_28190-t43_2">
        <property type="protein sequence ID" value="PTTG_28190-t43_2-p1"/>
        <property type="gene ID" value="PTTG_28190"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_28190"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031930">
        <property type="term" value="P:mitochondria-nucleus signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936:SF1">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN GUN1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936">
        <property type="entry name" value="PPR_LONG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OAV90863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_28190-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="522" mass="60114" checksum="6FB813DD30AF1454" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GFY6</accession>
    <name>A0A180GFY6_PUCT1</name>
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        <submittedName>
            <fullName evidence="5">TYR_PHOSPHATASE_2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_27956</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000080">
        <property type="protein sequence ID" value="OAV91484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GFY6"/>
    <dbReference type="EnsemblFungi" id="PTTG_27956-t43_1">
        <property type="protein sequence ID" value="PTTG_27956-t43_1-p1"/>
        <property type="gene ID" value="PTTG_27956"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_27956"/>
    <dbReference type="OrthoDB" id="266663at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057023">
        <property type="entry name" value="PTP-SAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22784">
        <property type="entry name" value="PTP-SAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="3">
        <location>
            <begin position="405"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="231"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="OAV91484.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_27956-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GG61</accession>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
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        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000077">
        <property type="protein sequence ID" value="OAV91680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GG61"/>
    <dbReference type="EnsemblFungi" id="PTTG_06517-t43_1">
        <property type="protein sequence ID" value="PTTG_06517-t43_1-p1"/>
        <property type="gene ID" value="PTTG_06517"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06517"/>
    <dbReference type="OrthoDB" id="2505838at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV91680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06517-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="98" mass="11181" checksum="D313F53F3FED6571" modified="2016-09-07" version="1">MESLLSPPLNIQLVATSPAKSYEVVQKALSAPLDDPDLLPYVIGGNTHQTKNRLYQIRDEVCLLRRNSARRGPSRDQHTSKKRKHDDPSKRRPRILLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GG76</accession>
    <name>A0A180GG76_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_12102</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000075">
        <property type="protein sequence ID" value="OAV91747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GG76"/>
    <dbReference type="EnsemblFungi" id="PTTG_12102-t43_1">
        <property type="protein sequence ID" value="PTTG_12102-t43_1-p1"/>
        <property type="gene ID" value="PTTG_12102"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12102"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV91747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12102-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="413" mass="47728" checksum="C3E3ED455B9B54F2" modified="2016-09-07" version="1">MESERALIAQRFEDLYDKYDALDLGYERGRPAPLMDENAVSKAASLRRLHSRILPELRRLVIKLRSLVITLYKSSKRPGDLQEEPGDLVELIFQTQSEIDSLFDQFIDAMNILRPDQYVKELFSRRADRVYSRLLTVALHFAGFCLSSCTLIQMSSEQTDSDSDSDSSDSKSDSDSHSDCESDCDSEDMTRCTNQKEMVNHDANVVCKKIGSALELIEGREFDIIQDYWARPKYRIDRKINDLFKVSNQTTTPSWQEPLSESVCEVAKWFAPIFKLSRIFFEKLSRRGTNRNQHPLIATMRFNDLDTIGELAHRVDSELEEIINILRQGDRAGLFATGRLLTQTLFKLTFHFDNPLHLIKSRFVPIVPGTNSSSTIQADWQHWFSLWNHWFILAITNVSKRTKYLSRTLSHQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GG99</accession>
    <name>A0A180GG99_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_03752</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000075">
        <property type="protein sequence ID" value="OAV91766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GG99"/>
    <dbReference type="EnsemblFungi" id="PTTG_03752-t43_2">
        <property type="protein sequence ID" value="PTTG_03752-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03752"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03752"/>
    <dbReference type="OrthoDB" id="10264544at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV91766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03752-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="192" mass="20638" checksum="3909BE7B3F8A1E20" modified="2016-09-07" version="1" fragment="single">MDPLAATPASPDTKKGDKSSTTDDDPPEEIARKRAEIASGSAGLKAAPGGPMAVQPSVGPPSVPKIDPNLANAQKKKDLYQTSQAEADPVVADNRSNTHFNKSLPGPIGDPSGLVRTRATNETATEADSELNLTIPAPPEQSYVSRKECYDSIQKWNGYAIATARSYPFKGSTRVVYQCDKSSYYRPHQKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GGX0</accession>
    <name>A0A180GGX0_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="9">CMGC/CDK/CDK5 protein kinase, variant</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_02655</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000073">
        <property type="protein sequence ID" value="OAV91871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GGX0"/>
    <dbReference type="EnsemblFungi" id="PTTG_02655-t43_2">
        <property type="protein sequence ID" value="PTTG_02655-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02655"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02655"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF46">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OAV91871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02655-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="221" mass="24942" checksum="4DB16BA70007FD9E" modified="2016-09-07" version="1">MKELKHPNIVRLYDVIHTETKLMLVFEFMDLDLKKYMDTHGERGALEAPVVRSFMYQVGIILTQTYRLKIAPSGSPHLDFCCPLNQLLKGTAFCHENRVLHRDLKPQNLLINKRGELKLADFGLARAFGIPVNTFSNEVVTLWYRAPDVLLGSRTYSTSIDVWSAGCIMAESLCSFISSFVCSLMLIDSNLVLSDKWGTSVPRSGQQRSANSNSEGAWHTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GLD1</accession>
    <name>A0A180GLD1_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="12">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PTTG_04940</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="15">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ELO family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="12"/>
    <dbReference type="EMBL" id="ADAS02000059">
        <property type="protein sequence ID" value="OAV92803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GLD1"/>
    <dbReference type="EnsemblFungi" id="PTTG_04940-t43_2">
        <property type="protein sequence ID" value="PTTG_04940-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04940"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04940"/>
    <dbReference type="OrthoDB" id="434092at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF134">
        <property type="entry name" value="ELONGATION OF FATTY ACIDS PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OAV92803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04940-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="285" mass="32543" checksum="E5AB66C0DB800A82" modified="2016-09-07" version="1">MPILWELSTKLFAQLPPVPSHFTQWEPPQSPLSTYSGVFAAIVTYLSIIFGGQQFKPLFMLHNILLSLGSLWLLLLMIEQVAPIVYNHGVFYSICHVNSWTPQLVTLYMINYYFKYWELLDTCFLVAKKKSLQFLHVFHHTATALLCFTQLGGRTSVSWVPISANLTVHVIMYYYYFTTSAFPGYKPWYKKALTTLQISQFVIDLFIVYFASYSYFAAEYLGWPTMGNCTGTEGAALFGCAILTSYLFLFISFYRKTYKQKSAKGRAPVSSSQAETDSSTVTKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GLE5</accession>
    <name>A0A180GLE5_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DUF159-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_01603</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the SOS response-associated peptidase family.</text>
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        <property type="protein sequence ID" value="OAV93445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GLE5"/>
    <dbReference type="EnsemblFungi" id="PTTG_01603-t43_1">
        <property type="protein sequence ID" value="PTTG_01603-t43_1-p1"/>
        <property type="gene ID" value="PTTG_01603"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_01603"/>
    <dbReference type="OrthoDB" id="2111841at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:protein-DNA covalent cross-linking repair"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="SOS response associated peptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036590">
        <property type="entry name" value="SRAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604:SF0">
        <property type="entry name" value="ABASIC SITE PROCESSING PROTEIN HMCES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="2" id="KW-0645">Protease</keyword>
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            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="OAV93445.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblFungi" id="PTTG_01603-t43_1-p1"/>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="Google" id="ProtNLM"/>
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        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
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    <reference evidence="2" key="1">
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                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000051">
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        <property type="gene ID" value="PTTG_12171"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12171"/>
    <dbReference type="OrthoDB" id="2495060at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008110041" description="Hydrophobin" evidence="1">
        <location>
            <begin position="25"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV93364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12171-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13091" checksum="1A346B86815172FC" modified="2016-09-07" version="1">MNFSTSMNAFIMMVAIVCVGLVNGKRGKDTAQRFGCPHEMVYGHCRWIDVDDNDYITWNVARAPRYGPGYSCRSVDTAYCCTDLNETHSVQGNGIKAVNNVAQFVNSDQCVAAKTHYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GMB7</accession>
    <name>A0A180GMB7_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ANK_REP_REGION domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_12162</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
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                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000045">
        <property type="protein sequence ID" value="OAV93926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblFungi" id="PTTG_12162-t43_2">
        <property type="protein sequence ID" value="PTTG_12162-t43_2-p1"/>
        <property type="gene ID" value="PTTG_12162"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12162"/>
    <dbReference type="OrthoDB" id="19174at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV93926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12162-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="196" mass="21376" checksum="2EEF136DBA88F776" modified="2016-09-07" version="1">MFFKQSNHPPPPCHPKKRAKISTPLFSVEDLDTAKLVIELGGDPRHKNSEGKTAAENLAEDYPEVCNYLRSLVGEPPLEESASQSEQEDPAESSLDPTNEMTKTLIDKVREMMTDAESNGIDTDSPELDLRLRELVTKTVDQSVDLGKLLASSGRDHSQSAAVVAQDLASVAEGEEPTEENKQAATGSDSNKRQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GME1</accession>
    <name>A0A180GME1_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_08324</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000044">
        <property type="protein sequence ID" value="OAV93977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GME1"/>
    <dbReference type="EnsemblFungi" id="PTTG_08324-t43_3">
        <property type="protein sequence ID" value="PTTG_08324-t43_3-p1"/>
        <property type="gene ID" value="PTTG_08324"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08324"/>
    <dbReference type="OrthoDB" id="10262287at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
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        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
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        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043155">
        <property type="entry name" value="VPS33_dom3b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV93977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08324-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="609" mass="67326" checksum="CB7D5A6DE367597C" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GME3</accession>
    <name>A0A180GME3_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HTH APSES-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_06232</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000047">
        <property type="protein sequence ID" value="OAV93751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GME3"/>
    <dbReference type="EnsemblFungi" id="PTTG_06232-t43_2">
        <property type="protein sequence ID" value="PTTG_06232-t43_2-p1"/>
        <property type="gene ID" value="PTTG_06232"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06232"/>
    <dbReference type="OrthoDB" id="5562739at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051642">
        <property type="entry name" value="SWI6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828">
        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR XBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="437"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV93751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06232-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="529" mass="56930" checksum="22F7416B2287A001" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GMF7</accession>
    <name>A0A180GMF7_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zinc finger ZPR1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_06242</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ZPR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000047">
        <property type="protein sequence ID" value="OAV93771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GMF7"/>
    <dbReference type="EnsemblFungi" id="PTTG_06242-t43_2">
        <property type="protein sequence ID" value="PTTG_06242-t43_2-p1"/>
        <property type="gene ID" value="PTTG_06242"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06242"/>
    <dbReference type="OrthoDB" id="308464at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.420:FF:000002">
        <property type="entry name" value="Zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1040:FF:000001">
        <property type="entry name" value="Zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1040:FF:000003">
        <property type="entry name" value="Zinc finger protein zpr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1040">
        <property type="entry name" value="ZPR1, A/B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.420">
        <property type="entry name" value="ZPR1, zinc finger domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004457">
        <property type="entry name" value="Znf_ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040141">
        <property type="entry name" value="ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042451">
        <property type="entry name" value="ZPR1_A/B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056180">
        <property type="entry name" value="ZPR1_jr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042452">
        <property type="entry name" value="ZPR1_Znf1/2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00310">
        <property type="entry name" value="ZPR1_znf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22794">
        <property type="entry name" value="jr-ZPR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03367">
        <property type="entry name" value="Zn_ribbon_ZPR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00709">
        <property type="entry name" value="Zpr1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="6">
        <location>
            <begin position="204"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="394"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV93771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06242-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="417" mass="46164" checksum="BFEC0517CFA495FA" modified="2016-09-07" version="1">MLLTSIPFFREVIVVSFRCPHCHHANNSIQSAGQIQPRGAVYTVKNVHRPADLNRQVIKSEHGVVRIKELELEIPKGHGKMTTVEGLLRDTIDDLSLNQPARKHLDPLVHAKIDELLARLAEVLQFRAQPLTIELEDITGNSFIEAIDGLNDPHWTKREFLRSPAQNAELGLAPQSAGQEQPPAGGLTDDYEGENPEEVYSFPATCGSCGHTLNTRMKKIEIPHFKEIILMSTNCAHCGYKDNEVKSATAIAPKGTKLVLRVADKEDLARDILKSDTAELTIPEIDLHLNPGTLGGRFTTLEGLLTQVFEELDQKVFATGDSLSTASPAHPSPGPEEGQNHSKSSMKIFMDKFKAILSVDTPFTVILDDPISNSFVQNIYAPDDDPAIEKTEYERSFEQNEELGLNDMRTENYAPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GNE2</accession>
    <name>A0A180GNE2_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RF_PROK_I domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_05259</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000042">
        <property type="protein sequence ID" value="OAV94240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GNE2"/>
    <dbReference type="EnsemblFungi" id="PTTG_05259-t43_2">
        <property type="protein sequence ID" value="PTTG_05259-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05259"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05259"/>
    <dbReference type="OrthoDB" id="2019491at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000070">
        <property type="entry name" value="Related to MRF1-peptide chain release factor, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050057">
        <property type="entry name" value="Prokaryotic/Mito_RF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804:SF7">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="4">
        <location>
            <begin position="168"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OAV94240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05259-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="308" mass="34818" checksum="C04132F0533350D6" modified="2016-09-07" version="1">MREIANTELRTVEERVPMTIERVRESLIEVSRPRPESYDQSALVEIKPGVGGVEASHFATMLMNLYSRLATRKRWHVKPVSVEYMTSGQGQDGLREAVLEIEGHAAYGSLRWEAGVHRVQRVPVMQNTASIHTSTASVIVLPLNPAGSEENPEENLFEEKDIRLETMRSQGAGGQHVNKTESAVRLTHIPTGTVVSMQDSRSQHQNRERAYMILRARLLDLKLRQKVIENRESRLSQVKNTNRSEKIRTYNFQQGRVTDHRIGLTLPRLNDVIDGGETLDIIWDGLEKDEESTIIQSICAANPSSHPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GNF5</accession>
    <name>A0A180GNF5_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rab-GAP TBC domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_12039</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000040">
        <property type="protein sequence ID" value="OAV94337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GNF5"/>
    <dbReference type="EnsemblFungi" id="PTTG_12039-t43_2">
        <property type="protein sequence ID" value="PTTG_12039-t43_2-p1"/>
        <property type="gene ID" value="PTTG_12039"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12039"/>
    <dbReference type="OrthoDB" id="10264062at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000077">
        <property type="entry name" value="TBC1 domain family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000102">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF502">
        <property type="entry name" value="SMALL G PROTEIN SIGNALING MODULATOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="479"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="554"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="556"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV94337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12039-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="801" mass="90820" checksum="F3DE75C57E620A1D" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GNG2</accession>
    <name>A0A180GNG2_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_03385</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000040">
        <property type="protein sequence ID" value="OAV94347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GNG2"/>
    <dbReference type="EnsemblFungi" id="PTTG_03385-t43_1">
        <property type="protein sequence ID" value="PTTG_03385-t43_1-p1"/>
        <property type="gene ID" value="PTTG_03385"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03385"/>
    <dbReference type="OrthoDB" id="2565191at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.80">
        <property type="entry name" value="E3 ubiquitin-protein ligase SopA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141571">
        <property type="entry name" value="Pentapeptide repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV94347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03385-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="249" mass="28157" checksum="8802583C2B5DAA38" modified="2016-09-07" version="1">MVPSIQPGRRKIDPSCRPQYQKLIDGDSIYTCSKFTSEREPRLGDSIFRPSKKFKKSLMVEHGPWFLDLEPVLERRDQGRHRLWKRQKNKRLKRISSAARPNPQQAGPQQANSQHADPQQTDPQLQTAYSQRTDRLTAEPQHTDLQPTDLQPTDLQPTDLQHTDLQQTDLRAAYLPQADPQHAISQHANLQQADLHQADDEDADRTITLAAFGTQGTCSLPIDNTVEFPPRAVTPGPSTIDPSRSSHVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GPK8</accession>
    <name>A0A180GPK8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_04399</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
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                <person name="Zeng Q."/>
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                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
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                <person name="Engels R."/>
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                <person name="Szabo L."/>
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                <person name="Szabo L."/>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000036">
        <property type="protein sequence ID" value="OAV94804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GQK4"/>
    <dbReference type="EnsemblFungi" id="PTTG_01052-t43_1">
        <property type="protein sequence ID" value="PTTG_01052-t43_1-p1"/>
        <property type="gene ID" value="PTTG_01052"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_01052"/>
    <dbReference type="OrthoDB" id="3643at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Oxoprolinase-like"/>
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    <dbReference type="PANTHER" id="PTHR11365:SF2">
        <property type="entry name" value="5-OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
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            <dbReference type="EMBL" id="OAV94804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_01052-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="219" mass="23935" checksum="36F9B88D79E69348" modified="2016-09-07" version="1">MALADRVVEAQTPSAVVYQPSGPARRALEDQLARLQAQVVDSLHKQGFEDRRIVVERFLNMRYDGTDTALMVLDPSSARPPNSGQGEPDYFDYLAAFKAAYRQQFGFILAPSVAVVCDDVRVRGSGKSSEAEGESVAAQLARIRFAPFALPPADEGGFAMVFFEQTMGRVRTPVLALEKFRPGDILDGPAVLLDQTQTVVVDPDASVRFLDAHVVIDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
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    <name>A0A180GQK8_PUCT1</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_05187</name>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000036">
        <property type="protein sequence ID" value="OAV94831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblFungi" id="PTTG_05187-t43_2">
        <property type="protein sequence ID" value="PTTG_05187-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05187"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05187"/>
    <dbReference type="OrthoDB" id="2509318at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV94831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05187-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="327" mass="37144" checksum="A7A289DD52CD0704" modified="2016-09-07" version="1">MPLSIDDLLYGDVDDPANFGGAHHDPTTPDCHHNSCSPSDEFETNNPNNIFDLPPNRGDVNMAGPSASYNLSDDITEIAKLACKVISVHLFVDSDFVRETAQLILLKPTIDAYSNNPHNDGTIPNSLFYLTLDTLDKQPDQWKEDHTAPSDVIDTLARLADYCRLIADLLKYRRSYLRDMLLTNTQETKKIKVNAPIPNRKDLLTKIYDNLPPKGHKLTEAKIAQQVKENYAMRAQICYCRLVMVQSLAHKSRNNSQWSDINDRLRILRGSSYTFQEHHSVIVLNKDFELFSHKRYYNEIDKTEFTVPTIEEVQASVDGGIVPAILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GQL0</accession>
    <name>A0A180GQL0_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_12103</name>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000038">
        <property type="protein sequence ID" value="OAV94562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GQL0"/>
    <dbReference type="EnsemblFungi" id="PTTG_12103-t43_2">
        <property type="protein sequence ID" value="PTTG_12103-t43_2-p1"/>
        <property type="gene ID" value="PTTG_12103"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12103"/>
    <dbReference type="OrthoDB" id="2506728at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV94562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12103-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="423" mass="47650" checksum="CBAD1C78B5689517" modified="2016-09-07" version="1">MRIMCLPDKALQTADRINLTLQPTLNLFTDSLTTVNRQLETVGSQAVRQSNIQDNQGLLLAKLEKDVRYLTIQANEQESRLAADKNEHMQMMCTLQNEFDTLNSSLAPIALMAPLLQAFLESQLRSSSNSKEPSGWCNTSYGQTEVLEKTVTRHSSAINMRSCAETRSLKRPETQAHIPKPQVVRPDSSVSLGKVRVNKPLEDSEVNVVQRPVQNESDDTQITRNQEPGDVSNKVEAEQEPTTTQASENQLYTKGEVHGRRDEVDERLTENLQRLPSSVARKSMSRRRSQIRPNSTQQESNTTDLAFPKAQSRKRTRSRTPTGLDQEHIVRSKSISYTQLRKISSQPTALDSPGGTAIPQDCHQSYRQSNQVIKHIQAHSTLHKVRTHPLAKDQDENVADFQHRLSHRACSFLAIVRPVLPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GQN3</accession>
    <name>A0A180GQN3_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
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        </recommendedName>
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    <gene>
        <name evidence="12" type="ORF">PTTG_03097</name>
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    <organism evidence="12">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="ADAS02000037">
        <property type="protein sequence ID" value="OAV94738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GQN3"/>
    <dbReference type="EnsemblFungi" id="PTTG_03097-t43_2">
        <property type="protein sequence ID" value="PTTG_03097-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03097"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03097"/>
    <dbReference type="OrthoDB" id="10250725at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="STKc_Nek2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001328">
        <property type="entry name" value="NEK protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF10">
        <property type="entry name" value="NIMA-RELATED KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="59"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="514"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="716"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="517"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="568"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="588"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="722"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="759"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OAV94738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03097-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="794" mass="87430" checksum="4D8F0FA6F04B8037" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GR05</accession>
    <name>A0A180GR05_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Checkpoint protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_00435</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the HUS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000034">
        <property type="protein sequence ID" value="OAV94954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GR05"/>
    <dbReference type="EnsemblFungi" id="PTTG_00435-t43_1">
        <property type="protein sequence ID" value="PTTG_00435-t43_1-p1"/>
        <property type="gene ID" value="PTTG_00435"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00435"/>
    <dbReference type="OrthoDB" id="337750at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044778">
        <property type="term" value="P:meiotic DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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            <dbReference type="EMBL" id="OAV94954.1"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
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    <sequence length="228" mass="24746" checksum="87F492122633E70A" modified="2016-09-07" version="1">MNNNIHVEVSTQAFASAVRQTSSSPTEIVIRLAKLGRTPYMSFVAKTVGRSGVEYQVEQQVVVKVLKPPDLKDLPELKIPTPDTIIALPKISDLCKVAERLKSLSDLITISASRNGGFRLGITTEHARVETEWRGLHSFHAPSTQVTQDGTPRPTSAFYEVTVKSRGLLRCLHANLGEVSMAYICEGSCVALYNFVGGNAQLSGIEDGSERTGSLATYLPAVNEDVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GS15</accession>
    <name>A0A180GS15_PUCT1</name>
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        <submittedName>
            <fullName evidence="12">HSF_DOMAIN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PTTG_05194</name>
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        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
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    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Homotrimer. Homotrimerization increases the affinity of HSF1 to DNA. Interacts with transcriptional coregulator SSA1 on chromatin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000033">
        <property type="protein sequence ID" value="OAV95072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GS15"/>
    <dbReference type="EnsemblFungi" id="PTTG_05194-t43_2">
        <property type="protein sequence ID" value="PTTG_05194-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05194"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05194"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="10">
        <location>
            <begin position="86"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
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        </location>
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        <location>
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            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
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            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
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            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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    <feature type="compositionally biased region" description="Polar residues" evidence="9">
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OAV95072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05194-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="834" mass="90687" checksum="A13909759A9C28D9" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GS16</accession>
    <name>A0A180GS16_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial glyco protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_05417</name>
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    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000036">
        <property type="protein sequence ID" value="OAV94773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GS16"/>
    <dbReference type="EnsemblFungi" id="PTTG_05417-t43_1">
        <property type="protein sequence ID" value="PTTG_05417-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05417"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05417"/>
    <dbReference type="OrthoDB" id="278212at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.280.10">
        <property type="entry name" value="Mitochondrial glycoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003428">
        <property type="entry name" value="MAM33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036561">
        <property type="entry name" value="MAM33_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826">
        <property type="entry name" value="COMPLEMENT COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826:SF1">
        <property type="entry name" value="COMPLEMENT COMPONENT 1 Q SUBCOMPONENT-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02330">
        <property type="entry name" value="MAM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54529">
        <property type="entry name" value="Mitochondrial glycoprotein MAM33-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV94773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05417-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="27246" checksum="25370C13B00CFD40" modified="2016-09-07" version="1">MISKPIWSLSRLAVRSRIPLSATLPARASPFLRSSPQACSQILSVQRHFSVGPVSRSAGETDSQLVDKLEAEISYEKDAESTEDPQWLKEFKADGTFQITDKPGSDEVTLTRKFGNEHIRIVFSCSDSETDEMNDELEEESEDDDKEAIGNCRIRTAISIMKPGKGGMVIDSSTDGASFDIENVSFYDDEKLALDETHESDWKRRGLYFGPTFIDLDEELQRSFAEFLEERAIGSELAACSRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GS18</accession>
    <name>A0A180GS18_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="2 9">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PTTG_10076</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the phage and mitochondrial RNA polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2 9"/>
    <dbReference type="EMBL" id="ADAS02000031">
        <property type="protein sequence ID" value="OAV95208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GS18"/>
    <dbReference type="EnsemblFungi" id="PTTG_10076-t43_1">
        <property type="protein sequence ID" value="PTTG_10076-t43_1-p1"/>
        <property type="gene ID" value="PTTG_10076"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_10076"/>
    <dbReference type="OrthoDB" id="276422at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001018">
        <property type="term" value="F:mitochondrial promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006390">
        <property type="term" value="P:mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.280:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1320.10">
        <property type="entry name" value="DNA-directed RNA polymerase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046950">
        <property type="entry name" value="DNA-dir_Rpol_C_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002092">
        <property type="entry name" value="DNA-dir_Rpol_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037159">
        <property type="entry name" value="RNA_POL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029262">
        <property type="entry name" value="RPOL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00940">
        <property type="entry name" value="RNA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14700">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01311">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00900">
        <property type="entry name" value="RNA_POL_PHAGE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00489">
        <property type="entry name" value="RNA_POL_PHAGE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase N-terminal" evidence="11">
        <location>
            <begin position="398"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="344"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="775"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1108"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1115"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1142"/>
            <end position="1151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OAV95208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_10076-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="1273" mass="142785" checksum="548AAEA5C045B7B3" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GS21</accession>
    <name>A0A180GS21_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_03610</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000030">
        <property type="protein sequence ID" value="OAV95340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="ADAS02000030">
        <property type="protein sequence ID" value="OAV95341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GS21"/>
    <dbReference type="EnsemblFungi" id="PTTG_03610-t43_3">
        <property type="protein sequence ID" value="PTTG_03610-t43_3-p1"/>
        <property type="gene ID" value="PTTG_03610"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_03610-t43_4">
        <property type="protein sequence ID" value="PTTG_03610-t43_4-p1"/>
        <property type="gene ID" value="PTTG_03610"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03610"/>
    <dbReference type="OrthoDB" id="2501270at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="100"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="249"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="332"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="136"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="259"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV95341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03610-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="377" mass="40671" checksum="B7367DC6761F1829" modified="2016-09-07" version="1">METAPSKNKAMPRLNSLAPAQNDPALRSSAHATHRPANSIGSHGAPPGVITTKQLQELQHRHQMQLALIKQEARELKEEKAFLVAQNLRLERELCLQHQPNVHGMGESKRADPSSRPSFSRSRSHPNADLRDSLHSLSSNSPTCSGSSCSSHPWASSGGPTTPSSLSPRSRHSRNASSASLAVPASHAPPPMSRPMSRRPSFSSAGPPATCAPDPALLLQRLRLADRQQKLAHSQNVLHPLRPLSVASNVCEEARPRSSRSNPGSRGVSRRNSLSGSGNDQPLKGILKPSTPIRPVSSCSNTIVGPPTRPPTRPSTGIGYYLDEAYDSITRPSGQRSTSCIYNQQPHHNSIPSHRSRRGSLDVHTGERPNPNHGCHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GSR2</accession>
    <name>A0A180GSR2_PUCT1</name>
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        <recommendedName>
            <fullName evidence="11">HOOK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_05494</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000033">
        <property type="protein sequence ID" value="OAV95003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GSR2"/>
    <dbReference type="EnsemblFungi" id="PTTG_05494-t43_1">
        <property type="protein sequence ID" value="PTTG_05494-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05494"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05494"/>
    <dbReference type="OrthoDB" id="49395at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030705">
        <property type="term" value="P:cytoskeleton-dependent intracellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22211">
        <property type="entry name" value="HkD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000217">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008636">
        <property type="entry name" value="Hook_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043936">
        <property type="entry name" value="HOOK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947:SF28">
        <property type="entry name" value="GIRDIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947">
        <property type="entry name" value="HOOK PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05622">
        <property type="entry name" value="HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19047">
        <property type="entry name" value="HOOK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116907">
        <property type="entry name" value="Hook domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HOOK N-terminal" evidence="7">
        <location>
            <begin position="38"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Hook C-terminal" evidence="6">
        <location>
            <begin position="248"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="177"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="264"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="489"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="884"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="307"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="545"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="634"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="828"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="264"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="887"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="921"/>
            <end position="930"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OAV95003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05494-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="958" mass="110390" checksum="607E874C3830731B" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GT35</accession>
    <name>A0A180GT35_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_26766</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000032">
        <property type="protein sequence ID" value="OAV95133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GT35"/>
    <dbReference type="EnsemblFungi" id="PTTG_26766-t43_1">
        <property type="protein sequence ID" value="PTTG_26766-t43_1-p1"/>
        <property type="gene ID" value="PTTG_26766"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_26766"/>
    <dbReference type="OrthoDB" id="2507588at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01228">
        <property type="entry name" value="EGGSHELL"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008110238" evidence="2">
        <location>
            <begin position="26"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV95133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_26766-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="369" mass="34863" checksum="FC4D576A44E31243" modified="2016-09-07" version="1">MIPGRSLLILSAMLAAGIFPHSLMARGDPKTVIAGHLIGARSNHDYTRPGAYLSRRKSVGISASKTKIALTSRDRLVRSSFGRRHNPKEEMLGGGLGVGGGGGFGGGGGVGGGGGVGGGGGVGGLGVGGNVGVGAGGGLGLGGGLGGGLGGNLGGGIGGGGGGGGGGGGGGGGGGGGVGGGWSEGSAAGGGGGVGFASGGGGLQEASISVGSGGGGGTGSGAAYGGSGLSAGGGFSSGSSSIQESQTSGYSYSSSQSSSMNYQSMREACSGLVNKIKKCQNSLGGAAGIGGSAQAYFEASIQAIRELGAEIQAVLTSFHTCSECAGSQIVREFIRQPFHSSLPPAKPPIPLMSIQLPQSMDSYDQYRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GTQ6</accession>
    <name>A0A180GTQ6_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PTTG_09564</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>adenosine(1779)/adenosine(1780) in 18S rRNA + 4 S-adenosyl-L-methionine = N(6)-dimethyladenosine(1779)/N(6)-dimethyladenosine(1780) in 18S rRNA + 4 S-adenosyl-L-homocysteine + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42780"/>
            <dbReference type="Rhea" id="RHEA-COMP:10234"/>
            <dbReference type="Rhea" id="RHEA-COMP:10236"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74493"/>
            <dbReference type="EC" id="2.1.1.183"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="9"/>
    <dbReference type="EMBL" id="ADAS02000029">
        <property type="protein sequence ID" value="OAV95353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GTQ6"/>
    <dbReference type="EnsemblFungi" id="PTTG_09564-t43_1">
        <property type="protein sequence ID" value="PTTG_09564-t43_1-p1"/>
        <property type="gene ID" value="PTTG_09564"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09564"/>
    <dbReference type="OrthoDB" id="74991at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052909">
        <property type="term" value="F:18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.480:FF:000002">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 8 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 8 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="1"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OAV95353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09564-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="238" mass="27304" checksum="E3DA89678BA3B7A1" modified="2016-09-07" version="1">MDPRMAFELSKRFQNAKDLSFKRKLEIIVGDFLKTELPYFDVCISNTPYQISSPLVFKLLEHRPIFRVAVLMFQKEFGLRLCARPGSKLWSRLSVNVQLYSKVSHLMNVGKANFRPPPLVESCVVKLEPLNPPPNIAFNEFDGLTRICFNRRNKTIRASFFASTATLNMLEANWKTWRSQQQSDSAIVTDQEEGENFSAKLETILQESGYSDSRSAKMDVDDFLSLLACFHKHGIHFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GTU8</accession>
    <name>A0A180GTU8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_02999</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000025">
        <property type="protein sequence ID" value="OAV95798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GTU8"/>
    <dbReference type="EnsemblFungi" id="PTTG_02999-t43_2">
        <property type="protein sequence ID" value="PTTG_02999-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02999"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02999"/>
    <dbReference type="OrthoDB" id="1263307at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
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    <dbReference type="InterPro" id="IPR052897">
        <property type="entry name" value="Sec-Metab_Biosynth_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017:SF11">
        <property type="entry name" value="ESTERASE_LIPASE_THIOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="11"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV95798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02999-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="242" mass="26511" checksum="82A68F1120BDD87E" modified="2016-09-07" version="1">MFDSVNITLTEAGYTTSIIELPSTGARHGLPQGERDDAEYIRCQILKEIDSGGDVAIVCHSYAGVPTCDAVKGLGIGERKQNQQAGGVCSLIFVAAFVLWSGTSVNELNDKHQQGEFHYKEGLIWPARPEDLFYNDLPSGFAVELSRLLQPHSAGAFTSPLDHEAYRFIPSSYLICTEDNAIPLAAQEEMLADKQADFLLVERIASGHLPFISRPQETVDFITKSLALVRTKHTPIHPGISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GTV1</accession>
    <name>A0A180GTV1_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein CPL1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_05880</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000025">
        <property type="protein sequence ID" value="OAV95809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GTV1"/>
    <dbReference type="EnsemblFungi" id="PTTG_05880-t43_2">
        <property type="protein sequence ID" value="PTTG_05880-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05880"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05880"/>
    <dbReference type="OrthoDB" id="439917at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048661">
        <property type="entry name" value="CPL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038955">
        <property type="entry name" value="PriA/CPL1_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35192:SF2">
        <property type="entry name" value="APPLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35192">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21671">
        <property type="entry name" value="CPL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008110290" description="Protein CPL1-like domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Protein CPL1-like" evidence="2">
        <location>
            <begin position="202"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV95809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05880-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="267" mass="29329" checksum="10DC244D2086EDC8" modified="2016-09-07" version="1">MKLSDSLLTVLSLLVAYFFDPVAGGAYDSKLNSVDNHMGQLDYKISELQSVAQTNGHLDVLQLCGSAKDSLRKAQSAWKNIASSYGDKPWLASDSTYKESVEEHWYTCGSKLEIVYGHPNVLPENGGHPSYSEPVEACKETYDTCQSSFKHIWNWEAPKPVASGAYYKRQNKARRDLESTDLSQCPSGETACPISPHSTGLECIDVQSELTSCGGCVTKHEGENCMEIPGAVGVGCHLGRCVVFSVHPSYFLGREGRPILRRRRVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GU23</accession>
    <name>A0A180GU23_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7143 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_06298</name>
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    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000020">
        <property type="protein sequence ID" value="OAV96296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GU23"/>
    <dbReference type="EnsemblFungi" id="PTTG_06298-t43_2">
        <property type="protein sequence ID" value="PTTG_06298-t43_2-p1"/>
        <property type="gene ID" value="PTTG_06298"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06298"/>
    <dbReference type="OrthoDB" id="2497581at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055567">
        <property type="entry name" value="DUF7143"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37592">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37592:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23631">
        <property type="entry name" value="DUF7143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7143" evidence="3">
        <location>
            <begin position="81"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV96296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06298-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="321" mass="35949" checksum="1F653909D722BF6C" modified="2016-09-07" version="1">MIPSIAGRPRIPRSLLLKAKFHFIVLFICASFNIPKTISTELLKNPSIFSTYNSSSFNFKSSNKSRIPCFISDVIGSNNSNGNSLYWKEKKQPIPGIPDLELNGVTYSSIDFQSSLDNKSSVGFAIDKFIFQLEHKVALEKGSGTGGSSQFEPGENLTDEEEHIRQGERRKQMAKDEVLLLTTYWKIYGAMDIALRVLSNQSAAKILSLSKGPGFVLNFQRARLGGKVREIEFYLTQILRNCLNCTVIDRKRLILLARASGVTNRTLSATETVNSTEKTEHHLTLTSGSGQSQRLLKVYCRTQAYFILAFILLFSMTSLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GUS9</accession>
    <name>A0A180GUS9_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="12">RNase_PH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PTTG_04846</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000026">
        <property type="protein sequence ID" value="OAV95733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GUS9"/>
    <dbReference type="EnsemblFungi" id="PTTG_04846-t43_2">
        <property type="protein sequence ID" value="PTTG_04846-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04846"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04846"/>
    <dbReference type="OrthoDB" id="2504340at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF2">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT MTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008110296" evidence="9">
        <location>
            <begin position="25"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="10">
        <location>
            <begin position="20"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OAV95733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04846-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="241" mass="25475" checksum="F9A7601604657765" modified="2016-09-07" version="1">MTRMKLNQWVSLFWTRSFFLRCTGIKVGLISQANGSCYIESGKSKVICAVYGPKPRSSLSNSASLSPLVISMRFAPFCMAGARMIPTALGGVETTVSQLTQQALMPALLPMSDSSLVEIHITVLEWDGPLSPGPCVLASSIALASAGIPTLGLVIPTTLALSKDVYLDPTATEAEAASAIFDLASIPAMGTVTHVGFRSTTDWKSAPIEADIFDRCLDLCKSNAEIIHGLASAALKDHLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GUT0</accession>
    <name>A0A180GUT0_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arrestin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_09726</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000021">
        <property type="protein sequence ID" value="OAV96274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GUT0"/>
    <dbReference type="EnsemblFungi" id="PTTG_09726-t43_1">
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        <property type="gene ID" value="PTTG_09726"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09726"/>
    <dbReference type="OrthoDB" id="3345971at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
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    </dbReference>
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        <property type="entry name" value="Arrestin-like_N"/>
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        <property type="entry name" value="Ig_E-set"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E set domains"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <end position="331"/>
        </location>
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        <location>
            <begin position="498"/>
            <end position="528"/>
        </location>
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        <location>
            <begin position="558"/>
            <end position="571"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="741"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="910"/>
            <end position="939"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00339"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="OAV96274.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09726-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="979" mass="107187" checksum="A0BFA7CEB93F96DF" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GUT7</accession>
    <name>A0A180GUT7_PUCT1</name>
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        <submittedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_12224</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000026">
        <property type="protein sequence ID" value="OAV95743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GUT7"/>
    <dbReference type="EnsemblFungi" id="PTTG_12224-t43_3">
        <property type="protein sequence ID" value="PTTG_12224-t43_3-p1"/>
        <property type="gene ID" value="PTTG_12224"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12224"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
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        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969:SF69">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12190)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969">
        <property type="entry name" value="RING ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING"/>
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        <property type="entry name" value="RING/U-box"/>
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        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50089"/>
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    </evidence>
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            <dbReference type="EnsemblFungi" id="PTTG_12224-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="419" mass="46951" checksum="D63F8543A3214DC7" modified="2016-09-07" version="1">MALAIPTGQPEHDLAGTSDLQEVIPSTRQSASWPDFQEQIEERRRSRRSRPGARAFSWIRRQLLSCTNPSMAHARPLAEHQAANIEQGAAYVWQVTILVQGKDWFLAIYHQNDLPIQCRQAVASAVPRPDLPTTTNVDLLISYPATIKNFEAQAANYESGAANHEPGAAHYESVAAHYESVAANYESGPGDGWRRTFMEVGGDWIVVLFDRTSLVNTYIESEGFSNRQRRRLEEAERAFDSHTITSGYIPFNEQQKLHTETRHGTVSLDEASQLLATTAASQPGESDRLADSYDDGGLRFNSKLKGIQHQKTAACSHQDEPTNQDRVTESPNGSSTANPPNHYKYSHFIVGKENQNQCAVCGDQFKSPKNAGKHQWCISEVTRIKKCRHDFHPKCIRPWIIEHGNRSCPLCRVFLDDNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GUT9</accession>
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        <submittedName>
            <fullName evidence="8">WD_REPEATS_REGION domain-containing protein</fullName>
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        <name evidence="7" type="ORF">PTTG_11776</name>
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    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat ESC family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000019">
        <property type="protein sequence ID" value="OAV96520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GUT9"/>
    <dbReference type="EnsemblFungi" id="PTTG_11776-t43_1">
        <property type="protein sequence ID" value="PTTG_11776-t43_1-p1"/>
        <property type="gene ID" value="PTTG_11776"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_11776"/>
    <dbReference type="OrthoDB" id="2495869at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051243">
        <property type="entry name" value="PcG_WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10253">
        <property type="entry name" value="POLYCOMB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="104"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="174"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV96520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11776-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="458" mass="50105" checksum="49F3ACE30F3AD2C9" modified="2016-09-07" version="1">MWNDVKFFPFGHMAPILQDMKNVAAVCGGNKIRVIKLDPIEEKCHLLYEIVDKSNTVTGGRAEIFNSISWSIDPVSLQPILAAGGVRGVIKLFDARTANELGMLYGHGGTIFALSFSPTHPHVLASASIDHTVRIWNTALPLKSSQTRSGSESQSLLSNWDNPPGQLVTILAGVGGHVAPVCSVAWHPIHPLLATGGMDNHVKIWYLSKLPGFPINSPLQDIQLPETLQTVDQKDSVSLPAPITSLPIFNSKHVHSHWVDQIMWASRLTPILVSKSSTISKRSRHPLAAYAFEEDLEPATRVCIWQPSILEDVFLGESAPSTPQDDEANVGGVDFDMYLSFKVRKPGLDQEQESVEWGLGMCLLEASGEIHLAILLGNHPHGIAEFMLSSLDNAPDSSTPSINLHPSGQHKTNKSEEIFRAIDATPISLEDNPAFVLRVGQKGSIELWKRKFSISSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GV74</accession>
    <name>A0A180GV74_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anaphase-promoting complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_04005</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC5 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000020">
        <property type="protein sequence ID" value="OAV96431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GV74"/>
    <dbReference type="EnsemblFungi" id="PTTG_04005-t43_2">
        <property type="protein sequence ID" value="PTTG_04005-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04005"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04005"/>
    <dbReference type="OrthoDB" id="2504561at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037679">
        <property type="entry name" value="Apc5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026000">
        <property type="entry name" value="Apc5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830:SF9">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12862">
        <property type="entry name" value="ANAPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008110330" description="Anaphase-promoting complex subunit 5" evidence="7">
        <location>
            <begin position="34"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 5" evidence="8">
        <location>
            <begin position="244"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OAV96431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04005-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="415" mass="46691" checksum="8707CFDB335D97E7" modified="2016-09-07" version="1">MRSPGQPLLTVTPIHLAILTLLTILADLQKTNKNQPEQELLLIISTELIFEVRAFREPLSRRTSTEDRTAINQNQQLESIAEFYQLIRAKAKHIDRLRLHQLLSSINQAISITFSVDGLYRFLNRLQALTSPPASEAELEIQPDSPVGLYVRRCRLYISKLEFDQLSDLASQLKSFCTTTRAHSDYFQSRAAQPITTPSPGHSFLHSDRIDHAPFSTFDHPRDVDGKEVDPIVQAYLDFTDSYAPDYNKSSIGLRKFYDIRSSVSGTLCQQTSAKMSHLSGSNHQQALLSLAKFYFSFEAYPTAMDALEEATRLARLAGDRTIIDQCTCLRRRIMYFSGLESEDGGVEIDQVPLAGPIQAQACLQSLRNLGNSDQKDRSKGKANASANANEWLYWDSNEEIFSVFKSLSKVGPTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GV79</accession>
    <name>A0A180GV79_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Trafficking protein particle complex subunit 6B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_26439</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000021">
        <property type="protein sequence ID" value="OAV96279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GV79"/>
    <dbReference type="EnsemblFungi" id="PTTG_26439-t43_2">
        <property type="protein sequence ID" value="PTTG_26439-t43_2-p1"/>
        <property type="gene ID" value="PTTG_26439"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_26439"/>
    <dbReference type="OrthoDB" id="941624at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14944">
        <property type="entry name" value="TRAPPC6A_Trs33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
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        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037992">
        <property type="entry name" value="TRAPPC6/Trs33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817:SF0">
        <property type="entry name" value="GEO08327P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV96279.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_26439-t43_2-p1"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
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    <name>A0A180GW69_PUCT1</name>
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        <recommendedName>
            <fullName evidence="1">SigF-like NTF2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_12052</name>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000021">
        <property type="protein sequence ID" value="OAV96233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="PTTG_12052-t43_1-p1"/>
        <property type="gene ID" value="PTTG_12052"/>
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    <dbReference type="OrthoDB" id="2344312at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057514">
        <property type="entry name" value="NTF2_SigF"/>
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    <dbReference type="PANTHER" id="PTHR35393">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35393:SF1">
        <property type="entry name" value="SNOAL-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24840">
        <property type="entry name" value="NTF2_SigF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SigF-like NTF2-like" evidence="1">
        <location>
            <begin position="47"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV96233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12052-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="176" mass="20592" checksum="46E84D51B74E72FF" modified="2016-09-07" version="1">MQSLSMYKQLAFSRPFSLDCFGFLKNARQSSRLPTSPRKHFLLSFRMDDPANQIRGIVRSLIEPYRHAVIGENVSKYFTPDALITNPFWSHARLSKSEIHVKSIYEIIRLFSINIRVHFQGVMFDETKEHCTIGRIILPITNNDCIERSYPMCLSSSRRWIQSYIIISLPTLEFRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GW92</accession>
    <name>A0A180GW92_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_26279</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000018">
        <property type="protein sequence ID" value="OAV96618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GW92"/>
    <dbReference type="EnsemblFungi" id="PTTG_26279-t43_1">
        <property type="protein sequence ID" value="PTTG_26279-t43_1-p1"/>
        <property type="gene ID" value="PTTG_26279"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_26279"/>
    <dbReference type="OrthoDB" id="2503462at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125:SF12">
        <property type="entry name" value="AT HOOK TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125">
        <property type="entry name" value="LP07818P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="560"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="612"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="641"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="678"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV96618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
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            <dbReference type="EnsemblFungi" id="PTTG_26279-t43_1-p1"/>
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    </evidence>
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        <source>
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    <name>A0A180GXY8_PUCT1</name>
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            <fullName evidence="5">PH domain-containing protein</fullName>
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        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
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                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">1-1 BBBD Race 1</strain>
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        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
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                <consortium name="EnsemblFungi"/>
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        <scope>IDENTIFICATION</scope>
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        <submittedName>
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                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
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                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
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    <reference evidence="2" key="2">
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
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        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
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                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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            <dbReference type="PubMed" id="27913634"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
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    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="OAV97239.1"/>
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        <property type="gene ID" value="PTTG_12493"/>
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<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
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                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
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                <person name="Gellesch M."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
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                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
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                <person name="Khalil H.B."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
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                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000006">
        <property type="protein sequence ID" value="OAV98575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H0J3"/>
    <dbReference type="EnsemblFungi" id="PTTG_25594-t43_1">
        <property type="protein sequence ID" value="PTTG_25594-t43_1-p1"/>
        <property type="gene ID" value="PTTG_25594"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_25594"/>
    <dbReference type="OrthoDB" id="2499753at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV98575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_25594-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="252" mass="28384" checksum="679F54F228734544" modified="2016-09-07" version="1">MDPKTNSLVTPKPSEPLLKKDFLPTPTKTNEIQNNPPAAVNNTSKPNQPTSRNLLSSQNMLNPITAKPLSTKIMQGVSSDEIDGMIERIKADIEPLLGRYQDQEKRINTLKESLHPHILSTRTAVSHLSHLASRAKKTSDLIDQQSGAFIQLAKEDIYKQIESDSVGREKDFSDSQSDLLGGDQLELARTKLLEANKKLEKHIARQRHYERKLAMHLSTSQSILLLSSFQVHVYLSALHRKRYLYEPELTSG</sequence>
</entry>
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            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000009">
        <property type="protein sequence ID" value="OAV98119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblFungi" id="PTTG_25807-t43_2">
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        <property type="gene ID" value="PTTG_25807"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_25807"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV98119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_25807-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="189" mass="21000" checksum="93651BC7BE869845" modified="2016-09-07" version="1">MLTIRRLGNSSWFNVAGVFAVTHTSVDEPDWDKKTVALMTQGKYYVAGGLKGIEDHACVRLTYRGERLNKLGYGKFYVLKGFLVGAKRHEMPFFMVDVNDKKEVPKPNPEGMVSVMVKHNGINENDPDNESMVVKYVVPQHLISGQAVDVFAVGRQILFRGTLAGWNRELDIVRVKVSGGALDVEASSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H0K3</accession>
    <name>A0A180H0K3_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
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    <gene>
        <name evidence="2" type="ORF">PTTG_11804</name>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000009">
        <property type="protein sequence ID" value="OAV98138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblFungi" id="PTTG_11804-t43_1">
        <property type="protein sequence ID" value="PTTG_11804-t43_1-p1"/>
        <property type="gene ID" value="PTTG_11804"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_11804"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008110509" evidence="1">
        <location>
            <begin position="20"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV98138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11804-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="279" mass="32401" checksum="ECF9A3D421EECEE4" modified="2016-09-07" version="1">MFFRFSVVALVCSITHVWGMNSFVPIKDVGDVGDIDRTGRLPFMMISPYDKSDLDVYYSISRPTHLSQNVRHTRLNKDDVLRTLEDQQEVDAAKILRELPRGWHMVVDRDFYSRAHWDHWRRQFVISETVTRKMADLINTLCTRKLDDRPSITIVPFENHVHIQYPVITSDQEIESYILRKDVQTERFITALSHAKPRDTELEGQILSASRWNTGKYDPSILDPSSPFKRWLHRDPRTEFQEIIKDGEAKAIQQAFAHMGGFPDLPGVPVYIGSAAREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H1L9</accession>
    <name>A0A180H1L9_PUCT1</name>
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            <fullName evidence="7">5'-3' exoribonuclease</fullName>
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    <gene>
        <name evidence="10" type="ORF">PTTG_08535</name>
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    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
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                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
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                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Khalil H.B."/>
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                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
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                <person name="Adiconis X."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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            <dbReference type="PubMed" id="27913634"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
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        <property type="protein sequence ID" value="OAV98681.1"/>
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        <property type="gene ID" value="PTTG_07700"/>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000004">
        <property type="protein sequence ID" value="OAV99044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H2L3"/>
    <dbReference type="EnsemblFungi" id="PTTG_11931-t43_2">
        <property type="protein sequence ID" value="PTTG_11931-t43_2-p1"/>
        <property type="gene ID" value="PTTG_11931"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_11931"/>
    <dbReference type="OrthoDB" id="2500854at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV99044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11931-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="390" mass="44704" checksum="BBC9B9ABB7944993" modified="2016-09-07" version="1">MVASQLISSRSKTDLVVEALDGLVRKYHFSLNQIFGEQVKNLTIDDMDEKTELLDELESILLPLIKQQINSILHSLDLRDCEILPTPDFELTLEILSNLDQTLERTISSIERISLESPMPAETHDYHLKHCKEFRCSRLLWRIKAVVEGHLCELFRNFIQFIRVWELSDSHPESLEHRTKIFQKETEVLRITAHSDHSIDAIIALFRKSDLEIIQEEWLAAAESLDSVLEFLIMLSNCTIDSKKEGIAGPADHRTNGQGAEIVGVAGLTIPLVKLARIFLNKLAKRTTEEYPFKLDTELNSETLNLLHQDPVSIAERLDQFSLESTSLILAAHLTPLPHQSQWCSSEPHFKTWLLTLKNLWYRAMEHFLNGLDCVGGGEYEQPRQQEPEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H3M2</accession>
    <name>A0A180H3M2_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Kinesin motor domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_04413</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000002">
        <property type="protein sequence ID" value="OAV99596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H3M2"/>
    <dbReference type="EnsemblFungi" id="PTTG_04413-t43_1">
        <property type="protein sequence ID" value="PTTG_04413-t43_1-p1"/>
        <property type="gene ID" value="PTTG_04413"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04413"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF9">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="3">
        <location>
            <begin position="56"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="455"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="494"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="587"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="633"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="421"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="462"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="512"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="138"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV99596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04413-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="654" mass="70842" checksum="5EE164BECEDF7399" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H423</accession>
    <name>A0A180H423_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1764 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_00476</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1089"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1122"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1141"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1158"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV99393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04545-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="1198" mass="130060" checksum="5FEDEE7F37454F89" modified="2016-09-07" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H515</accession>
    <name>A0A180H515_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_00531</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000002">
        <property type="protein sequence ID" value="OAV99679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H515"/>
    <dbReference type="EnsemblFungi" id="PTTG_00531-t43_2">
        <property type="protein sequence ID" value="PTTG_00531-t43_2-p1"/>
        <property type="gene ID" value="PTTG_00531"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00531"/>
    <dbReference type="OrthoDB" id="2125396at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV99679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00531-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="488" mass="54969" checksum="D07D4D0EDD14A258" modified="2016-09-07" version="1">MTSSFPVRLATITLANCSLVCRQWYRLANPLMYSSPMINMGSRLRALSFLDRLVDELLEYELELMDSVKREPRPGSLIDQMRRLMFFGEGRWRHRASFDSRPDVVDRSTLHEILNRLWCCTHLEALVFVELPFTNPLHRPLVNRYPASLSTLILLTPLSHFELLFVLRDLPSLRHLCIGSHDDTSTVPAEHHTLFSRDQLPSSNLRSFGISARTLEAWLRTGMPWYQSILNLLVVPSVRSITTLHLELADEDDERHLLAILGRPPASNPDRTLDALSSSPSVGDTPGPERLFKLETVFLRGLSSVLMAESLSRLQGIRKLCLSSIYGTALRCPSPAAPEQHPEEPSLPLDPESLGGLESQLTRITVGPTARRTLSSRNFVWQSIPSSLEELSIDITRLSDHGPWNRRSADQLTRTLTALITSQQCPNLRSLGSILADPAFEFGIFGPNRLSPPLSDFVHAAQSHHVRLVQSFWCDSFVVNPSGTDWYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H526</accession>
    <name>A0A180H526_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="8">WD_REPEATS_REGION domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_00575</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat MORG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000002">
        <property type="protein sequence ID" value="OAV99612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H526"/>
    <dbReference type="EnsemblFungi" id="PTTG_00575-t43_2">
        <property type="protein sequence ID" value="PTTG_00575-t43_2-p1"/>
        <property type="gene ID" value="PTTG_00575"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00575"/>
    <dbReference type="OrthoDB" id="1068471at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051980">
        <property type="entry name" value="WD_repeat_MORG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842:SF3">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING PROTEIN 83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="57"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="99"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV99612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00575-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="227" mass="25053" checksum="51747D33C3EB8A9B" modified="2016-09-07" version="1">MDSKATHTSPALQCQLEHKGIVHVATFNHGAQYVLTGGQDRTIRLWNAKTGNFIKDYSGHGYEILGIAVSPNNSFLGSVGGDRSVFYWDVSTGAILRRFSGHTGLINACAFSFDGNLLISGSFDASIRVWDIKSQQRHPIQIFQDAKDSITSIIVSGSEFITGSVDGYVRSYDVRMGQVMEDYFEGYSLFCFIPCVRQTSCNIDSPAERRENNGRVYAELDDPTDGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H548</accession>
    <name>A0A180H548_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="17">AAA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PTTG_05935</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="17">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000001">
        <property type="protein sequence ID" value="OAW00086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H548"/>
    <dbReference type="EnsemblFungi" id="PTTG_05935-t43_1">
        <property type="protein sequence ID" value="PTTG_05935-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05935"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05935"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000175">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF97">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE YME1L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="385"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="166"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OAW00086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05935-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="788" mass="85554" checksum="F53FD1C78242F347" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H5Q5</accession>
    <name>A0A180H5Q5_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_25165</name>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000001">
        <property type="protein sequence ID" value="OAW00105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H5Q5"/>
    <dbReference type="EnsemblFungi" id="PTTG_25165-t43_2">
        <property type="protein sequence ID" value="PTTG_25165-t43_2-p1"/>
        <property type="gene ID" value="PTTG_25165"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_25165"/>
    <dbReference type="OrthoDB" id="2498631at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="692"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="526"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="575"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAW00105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_25165-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="758" mass="85435" checksum="22F274F86F83B9EA" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H5Q7</accession>
    <name>A0A180H5Q7_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_25133</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000001">
        <property type="protein sequence ID" value="OAV99998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H5Q7"/>
    <dbReference type="EnsemblFungi" id="PTTG_25133-t43_1">
        <property type="protein sequence ID" value="PTTG_25133-t43_1-p1"/>
        <property type="gene ID" value="PTTG_25133"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_25133"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40903">
        <property type="entry name" value="GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40903:SF1">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008110653" evidence="2">
        <location>
            <begin position="21"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="342"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV99998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_25133-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="444" mass="39514" checksum="36B33F7A0640DE22" modified="2016-09-07" version="1">MHLTSVVLSASILSASNSLAVPLGGYTNEDQSYGGSGYGSGGSIGGFSPAGMLGGIFGGGVGGSANGGVFGGLGGGGGGGLGGGLGGGLGGGLGGGLGGGVRGGVGGGVGGGAAGGLGGGLGAGLGGGVGGGLGGNFLSRSSAGGGLFGGLGGGASGGLGGDLHLGGDLHGGAGGGFGGIPFIGGGGAGGLGGDLHLGGGLSGGAAGGLGGGLGGGAAGGLGAGFGGGAIGRSVGVVGLPRPVGYAAGPAFVPQGPPTGLYAPPLLVAPPPVAGRVITPAVPAPGPLVVPNLPVVPARYAAGPPAVARPNTPYVPVSPPAGPPAVASPNTPYVPVSPPAGPPAVASPNTPSVPVSPPAGPPAVASPNTPYVPVSPPAGPPVVAHVNTPYVPVSPPALHVPAPYVAAPLNTPYVAAPVPGIVSHAPAPGAYATGPIAHGGGYGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H5R1</accession>
    <name>A0A180H5R1_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="9">TAF domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_12709</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF6 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000002">
        <property type="protein sequence ID" value="OAV99852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H5R1"/>
    <dbReference type="EnsemblFungi" id="PTTG_12709-t43_1">
        <property type="protein sequence ID" value="PTTG_12709-t43_1-p1"/>
        <property type="gene ID" value="PTTG_12709"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12709"/>
    <dbReference type="OrthoDB" id="361039at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08050">
        <property type="entry name" value="TAF6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.770:FF:000007">
        <property type="entry name" value="Chromosome 10, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.770">
        <property type="entry name" value="TAF6, C-terminal HEAT repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037796">
        <property type="entry name" value="TAF6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011442">
        <property type="entry name" value="TAF6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046344">
        <property type="entry name" value="TAF6_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10221">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10221:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07571">
        <property type="entry name" value="TAF6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAF6 C-terminal HEAT repeat" evidence="7">
        <location>
            <begin position="127"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="401"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="411"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="430"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OAV99852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12709-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="440" mass="49244" checksum="C6DD000175F3DA4E" modified="2016-09-07" version="1">MRHSKRTNLLVEDVDYALRAKNIEPLWGFASTDTLSFRRTTSAVGNLYFIDEEEIDLTKVLAAELPPVPQETSYTAHWLAVEGVQPAIPQNPTPAELKSHPAFSGLQSSAIPSSSKQPPESQNLTTKEHLSRELRLYFDRVTAAALSNDQSSRNAALASLSGDPGLHQLVPYLIQFAAEKITTTLSHPEPSMQYLRDALQILESILSNPHSYLEPYLHQILPSILTCLLSSSFPSSPVTDDLEREIRCTAASLLKSQLNRYQHSYPTLRTRILKTLTKSLIDPQSTDRNQLGAIVGVKYLGLEPTKTVLSQNIKAFGESLDLSLSEGKADQIRLDNLIKETLKIMSEAYSEQQIHRKTNSKSKKKLRSIPAQDELEAEVGVQFAARLMSTSDEEETRLAYTFIKESTQTSDTDDSEEDEERSEHAEEEETTKMDVVEEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H5V0</accession>
    <name>A0A180H5V0_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PTTG_10050</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000001">
        <property type="protein sequence ID" value="OAV99891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="ADAS02000001">
        <property type="protein sequence ID" value="OAV99892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H5V0"/>
    <dbReference type="EnsemblFungi" id="PTTG_10050-t43_1">
        <property type="protein sequence ID" value="PTTG_10050-t43_1-p1"/>
        <property type="gene ID" value="PTTG_10050"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_10050-t43_3">
        <property type="protein sequence ID" value="PTTG_10050-t43_3-p1"/>
        <property type="gene ID" value="PTTG_10050"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_10050"/>
    <dbReference type="OrthoDB" id="2139348at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF31">
        <property type="entry name" value="MITOCHONDRIAL ORNITHINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="41"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="154"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OAV99892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_10050-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="27912" checksum="08ADCC2FCD07881B" modified="2016-09-07" version="1">MPVVGAMAENATLFLVYGQVQQLIRRLAFPEQAHKRRPPPLPLPYVALSAACGGAMASFILTPIELVKCKMQVQQIGATKENPKRLAGPVAIVRSVIKKEGISGLWLGQTGTLLRETGGSAIWFCTFESVAALFVARRQESVLEPSKLLSKDDLSSPELMISGATAGIFYNFVFFPADSIKSTMQTASELAQPDHLPKSLASNQQSPRISFLDVGRQIIKTRGWKGLYAGCGITCLRSAPSSALIFWIVSRLESRFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H667</accession>
    <name>A0A180H667_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MT-A70-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_01525</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MT-A70-like family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000001">
        <property type="protein sequence ID" value="OAW00179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H667"/>
    <dbReference type="EnsemblFungi" id="PTTG_01525-t43_1">
        <property type="protein sequence ID" value="PTTG_01525-t43_1-p1"/>
        <property type="gene ID" value="PTTG_01525"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_01525"/>
    <dbReference type="OrthoDB" id="61116at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF4">
        <property type="entry name" value="N(6)-ADENINE-SPECIFIC METHYLTRANSFERASE METTL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="42"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAW00179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_01525-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="53834" checksum="D80992218C7837E8" modified="2016-09-07" version="1">MSQRGEPSSVLIEILLPEKNFTAYLLDPFVPLLRGFDQSTRVTVSRPPSEPHICTPTSFKAPCSTPKHSGNIRPGKRQRRNIEPSPALEPLCQPSSGETSQSHPLNGFQLEAAHHANIKGDIQHAVDAIRERWINECNQNGHSEWWSDPHPLRHPAIARLEWQSSSDFKCSDFWSDLTRDSFQVRNTQFDSDQLSIRVSDYGITCTNPSTTAIGQAFLPDKKCSITLPKRSGFSLATMENFKSEVLGLNHSSGWDAVVIDPPWQNKSATRGGKYRTVELYDLFKMDLPGILGDNGGKRALIAVWVTNRPKYRRFLKSKFLPDCHVQGPYSEWYWIKITASPTSDDKPILHQGGKPLFDLASNSPRRCYEGLVLGWYLPPSLRSEPRSTELPSKIFLSVPLGHSRKPNIIDLLAPHLPSDPSVLELFSRSVSGLASFGNQLETDSVRPMKGIWHSVGDESPRFMATPWVDSYKKNQSPSDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4EIM4</accession>
    <name>A0A0C4EIM4_PUCT1</name>
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        <submittedName>
            <fullName evidence="4">DUF4110 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_00591</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000450">
        <property type="protein sequence ID" value="OAV87350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4EIM4"/>
    <dbReference type="EnsemblFungi" id="PTTG_00591-t43_2">
        <property type="protein sequence ID" value="PTTG_00591-t43_2-p1"/>
        <property type="gene ID" value="PTTG_00591"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00591"/>
    <dbReference type="OMA" id="HIYNIAR"/>
    <dbReference type="OrthoDB" id="4447at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025183">
        <property type="entry name" value="DUF4110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052588">
        <property type="entry name" value="Kelch_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46063">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46063:SF1">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13422">
        <property type="entry name" value="DUF4110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4110" evidence="2">
        <location>
            <begin position="542"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="600"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV87350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00591-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="627" mass="71483" checksum="C9CDDA7A8413D53A" modified="2015-04-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4EJN0</accession>
    <name>A0A0C4EJN0_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_00951</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000019">
        <property type="protein sequence ID" value="OAV96489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4EJN0"/>
    <dbReference type="EnsemblFungi" id="PTTG_00951-t43_2">
        <property type="protein sequence ID" value="PTTG_00951-t43_2-p1"/>
        <property type="gene ID" value="PTTG_00951"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00951"/>
    <dbReference type="OMA" id="GRAANQY"/>
    <dbReference type="OrthoDB" id="2501782at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV96489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00951-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="234" mass="26312" checksum="1343480F4D6C7B89" modified="2015-04-01" version="1">MSKPTSTTSIGQLRNRRRTDLPPSNPPNERRNLEGLVGSIAGRAANQYHRIQNLFRSSDQRLDHRLAQDRAGINPQASSSSSNNPNFSSSSTQSTKRFFRTPLQAAPLQARVGEIEKILGRLELLLQEHSHRLALLEGETEQLASRSGTIRMNAPDTPMSNPPQDPELSPQPFPTNLSHLEPAFVQSLSDRLERMEALIELKEKNKTRAKSRANKLTRFMGLDDQSHPNKRPRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4EQY3</accession>
    <name>A0A0C4EQY3_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_03194</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
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                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
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                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
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                <person name="Heiman D."/>
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                <person name="Larson L."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
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                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
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                <person name="Chapman S.B."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
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                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
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            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
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                <consortium name="EnsemblFungi"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
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    <dbReference type="OrthoDB" id="2497746at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
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    </feature>
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        <source>
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    </evidence>
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        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="EnsemblFungi"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
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                <person name="Feldgarden M."/>
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                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
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                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
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                <person name="Saif S."/>
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                <person name="Shenoy N."/>
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            <strain evidence="1">1-1 BBBD Race 1</strain>
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    <reference evidence="1" key="2">
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">1-1 BBBD Race 1</strain>
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        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
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                <consortium name="EnsemblFungi"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_29787-t43_2-p1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
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<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNEP1R1 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000830">
        <property type="protein sequence ID" value="OAV86757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G281"/>
    <dbReference type="EnsemblFungi" id="PTTG_02868-t43_2">
        <property type="protein sequence ID" value="PTTG_02868-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02868"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02868"/>
    <dbReference type="OrthoDB" id="5599171at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0071595">
        <property type="term" value="C:Nem1-Spo7 phosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019168">
        <property type="entry name" value="NEP1-R1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005605">
        <property type="entry name" value="Spo7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20996">
        <property type="entry name" value="NUCLEAR ENVELOPE PHOSPHATASE-REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20996:SF1">
        <property type="entry name" value="NUCLEAR ENVELOPE PHOSPHATASE-REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03907">
        <property type="entry name" value="Spo7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OAV86757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02868-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="201" mass="22751" checksum="92B2D43AAC1826D5" modified="2016-09-07" version="1">MLSASNDNGSNTKCLAFLVTIIAASLLLAYKTFIVISPYKLIHYLYVALFLVSITTLILFFATGMYTDQIAYAYKFIPQTNRALRPFNMFLITPSTARSLTRTNQSGSVWSLLGRVSLGLLIDGLLGKPFKQLFRRRPGPSALARPANAPPTDLLHHKEENINLKNINPRGEILFSSAVNPHFIDQYKLYRNQWEIRQKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G353</accession>
    <name>A0A180G353_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase</fullName>
            <ecNumber evidence="3 14">2.4.1.258</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Dol-P-Man-dependent alpha(1-3)-mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PTTG_00189</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="17">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 14">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the first dolichyl beta-D-mannosyl phosphate derived mannose in an alpha-1,3 linkage to Man(5)GlcNAc(2)-PP-dolichol to produce Man(6)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29527"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="Rhea" id="RHEA-COMP:19517"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="ChEBI" id="CHEBI:132516"/>
            <dbReference type="EC" id="2.4.1.258"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12 14">
            <dbReference type="Rhea" id="RHEA:29528"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the glycosyltransferase ALG3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.258" evidence="3 14"/>
    <dbReference type="EMBL" id="ADAS02000595">
        <property type="protein sequence ID" value="OAV87091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G353"/>
    <dbReference type="EnsemblFungi" id="PTTG_00189-t43_1">
        <property type="protein sequence ID" value="PTTG_00189-t43_1-p1"/>
        <property type="gene ID" value="PTTG_00189"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00189"/>
    <dbReference type="OrthoDB" id="20028at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052925">
        <property type="term" value="F:dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007873">
        <property type="entry name" value="Glycosyltransferase_ALG3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646:SF0">
        <property type="entry name" value="DOL-P-MAN:MAN(5)GLCNAC(2)-PP-DOL ALPHA-1,3-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646">
        <property type="entry name" value="NOT56 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05208">
        <property type="entry name" value="ALG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OAV87091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00189-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="310" mass="36185" checksum="623D2E597CEE4B5B" modified="2016-09-07" version="1">MAFVLAIYKRCESLPTYAIFSLVLSKRLHSIYVLRLFNDGVAMMGLYISIWCLVTRRWNWSALWFSLALSIKMNILLFFPAFGFVYFQTVGLLKTINLMLIIILVQAWLAIPFLIEFPREYLSKAFEFKRVFEYQWTVNWRMIPQSDFLSPGFASFLTAVHLSTLVCFAWTKWSRAFGEDGLISLLVRGLQNWGRPAAKRPNVLTADYIVNVCFTSNLIGIICARTLHYQFYSWYAHQIVFLAWQTPYPIFLRILLMMSIEYSWNVFPSTAISSTILLVSNLLLLFGTWWTFEEAYSFVTIAFKKKPKVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G384</accession>
    <name>A0A180G384_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_11382</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000745">
        <property type="protein sequence ID" value="OAV86882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="ADAS02000745">
        <property type="protein sequence ID" value="OAV86883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G384"/>
    <dbReference type="EnsemblFungi" id="PTTG_11382-t43_1">
        <property type="protein sequence ID" value="PTTG_11382-t43_1-p1"/>
        <property type="gene ID" value="PTTG_11382"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_11382-t43_2">
        <property type="protein sequence ID" value="PTTG_11382-t43_2-p1"/>
        <property type="gene ID" value="PTTG_11382"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_11382"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV86882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11382-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="220" mass="24731" checksum="E66C034156E4C323" modified="2016-09-07" version="1">MDVNKESTRYCRFREKVLRHLDTLHDDFKLGGILNNEEALGQIVWIGQLIKPSGITMYPFPDKYPVFRRCLLRMAKGVRAAVRIRFDDDYMEKTCHPPRTPSLSSASQTDDHLCSTDSETDERPHSSEADTPPRSNGDSPTADHSDVRSPNEPLNNPSISPPAKPGVEEGLSDVQSPNKPLENISISPPAEPRVEENTLTVDHNDIPLAKRQRLHQMESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G3X4</accession>
    <name>A0A180G3X4_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_29490</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000481">
        <property type="protein sequence ID" value="OAV87298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G3X4"/>
    <dbReference type="EnsemblFungi" id="PTTG_29490-t43_1">
        <property type="protein sequence ID" value="PTTG_29490-t43_1-p1"/>
        <property type="gene ID" value="PTTG_29490"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_29490"/>
    <dbReference type="OrthoDB" id="6369095at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV87298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_29490-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="33462" checksum="DC64A8D4392FFB9F" modified="2016-09-07" version="1">MTYRENVLIVSNMNIPYIRVTLWKSSLYINLESITPNRLGGEKRVAMLNDRPDDVWDNWTIGKYSDGIDRVYGELSDVASRLYGNAAEYFSMRCMIVRNLEDRRSINSKLAAMHPSKAFVCKAGVVAEIDGIESMSIISKSLLLEKIRPDITLIKLKVGMPIILTKDCTHSQAGEAMVAMKKGSRLVVVAVKESMITAKMITPGRVGMELKIGRTMLYCDDVNNQDYAYLKHQFPVVLGYANAEGRWNIVAGAGSVRVEGTLAAGWRVWTGPMDAVGAGDVVARGGKMVAIGRLRRRSRVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G3Z9</accession>
    <name>A0A180G3Z9_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Dopey_N domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PTTG_05261</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DOP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000440">
        <property type="protein sequence ID" value="OAV87371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_05261-t43_2">
        <property type="protein sequence ID" value="PTTG_05261-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05261"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05261"/>
    <dbReference type="OrthoDB" id="297643at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040314">
        <property type="entry name" value="DOP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056457">
        <property type="entry name" value="DOP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007249">
        <property type="entry name" value="DOP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056458">
        <property type="entry name" value="TPR_DOP1_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042">
        <property type="entry name" value="DOPEY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042:SF24">
        <property type="entry name" value="PROTEIN DOPEY-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24598">
        <property type="entry name" value="DOP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04118">
        <property type="entry name" value="Dopey_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24597">
        <property type="entry name" value="TPR_DOP1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DOP1 N-terminal" evidence="8">
        <location>
            <begin position="89"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like middle TPR" evidence="9">
        <location>
            <begin position="392"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like C-terminal" evidence="10">
        <location>
            <begin position="1332"/>
            <end position="1675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1148"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1429"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1148"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1452"/>
            <end position="1462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046326"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04118"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF24597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="OAV87371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05261-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="1675" mass="187715" checksum="DEDCC79A1CD5A48F" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G476</accession>
    <name>A0A180G476_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_29414</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000384">
        <property type="protein sequence ID" value="OAV87485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G476"/>
    <dbReference type="EnsemblFungi" id="PTTG_29414-t43_1">
        <property type="protein sequence ID" value="PTTG_29414-t43_1-p1"/>
        <property type="gene ID" value="PTTG_29414"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_29414"/>
    <dbReference type="OrthoDB" id="3061527at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV87485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_29414-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="473" mass="53464" checksum="71EC5DFBAFBC74D8" modified="2016-09-07" version="1" fragment="single">MLSKNGTDGIPNTTNAQESLHRLYYMISRGRTSLMHGMVELFSFVKMLRDDWTAVMKGVSIKYGAKKNTEVGVSLGMTRKRQRYQNDGRPPDTTDALAEPDPKKAKTERAPKVVKTPGQGYCSYVAVPDNPSRSNRCWMAAALESLYALYTPLWQQGISGKGTDLFTALLLHFSSRVTYELNLTGSIRSTLTRGQNKLWEMANSKHPASFIRGEFSSCDFFIEILLDPLNHKKNDKFRKLFGFIEDRQFSCQALPGTRQPAEQTRRNSHVISIRPEMFLSSNTAYADVGQLFNVWTTEGIQINCGRVCRSCPEANQPLFKPAAPKKTKGRKPKRLNSEVELVGPQSNNGHNLLEISKLTFEDELPPLHLNIHLEVMHIADDRARRDFMGSTDWPFKLSIGGHTFTLMSRGYWANDHYWVKMLKSSGGMVGVWLHNDARNDGFAQLVNRVPGSISGVSPDTSWLMYSRMWTPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G488</accession>
    <name>A0A180G488_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_08188</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
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                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
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                <person name="Larson L."/>
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            <strain evidence="6">1-1 BBBD Race 1</strain>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
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                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">Isolate 1-1 / race 1 (BBBD)</strain>
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</entry>
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    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
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                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
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                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
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                <person name="Pearson M."/>
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                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">1-1 BBBD Race 1</strain>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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        <property type="gene ID" value="PTTG_28807"/>
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                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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            <strain evidence="8">Isolate 1-1 / race 1 (BBBD)</strain>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
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<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
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                <person name="Earl A."/>
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                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000135">
        <property type="protein sequence ID" value="OAV89250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G9F7"/>
    <dbReference type="EnsemblFungi" id="PTTG_01861-t43_1">
        <property type="protein sequence ID" value="PTTG_01861-t43_1-p1"/>
        <property type="gene ID" value="PTTG_01861"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_01861"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051140">
        <property type="entry name" value="GATA_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658:SF18">
        <property type="entry name" value="PROTEIN GAT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PAS" evidence="6">
        <location>
            <begin position="46"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="GATA-type" evidence="7">
        <location>
            <begin position="314"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="206"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OAV89250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_01861-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="39322" checksum="C327BBE7C275ACC4" modified="2016-09-07" version="1">MATTTTSSSHQITFQPNNPLASLIARPKSPKSKPMTHMLSQDFKRRKDWHKRLVEDVMDVLQVIGPTGELLFVSESMARLTGFPTSELLGNQISNWCFEEEDRLNLEREIKHCFSPGSSGIINFYCRFKKKDSPGCMIFEISGHLINVKTNPGVLRGETKSEKQVIIINARPYPTSCGRLTDEFLELMLENEAMMRVMNSRVGVGTMRGDEGVESSSSGQALERANGATTTTTMTGKNEAENQGEGKKKNNNNNKNEEPDEAAEDDGQHLAKESELEDELHLPFPAPPPPPPLPPVDPSEPAPRPRARGPNKRDRADFLCLDCGVTQSPEWRKGPMGRKTLCNACGLRYAKKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GA48</accession>
    <name>A0A180GA48_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Geranylgeranyl transferase type-2 subunit beta</fullName>
            <ecNumber evidence="3 11">2.5.1.60</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PTTG_08985</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="14">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="3 11"/>
    <dbReference type="EMBL" id="ADAS02000136">
        <property type="protein sequence ID" value="OAV89202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GA48"/>
    <dbReference type="EnsemblFungi" id="PTTG_08985-t43_2">
        <property type="protein sequence ID" value="PTTG_08985-t43_2-p1"/>
        <property type="gene ID" value="PTTG_08985"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08985"/>
    <dbReference type="OrthoDB" id="5428259at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02894">
        <property type="entry name" value="GGTase-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000012">
        <property type="entry name" value="Geranylgeranyl transferase type-2 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026873">
        <property type="entry name" value="Ptb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF11">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="12">
        <location>
            <begin position="13"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OAV89202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08985-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="325" mass="36120" checksum="B43BBD30E4056DDC" modified="2016-09-07" version="1">MSDSPAEFLPTKLLAEKHIEYIQSLDQNRNSLAYHLTEHLRMNGVYWGLASTLLLDRPDGLPRQEMINWVMSCWEPESGGFRPHPGHDPHIHSTLSAIQILTMQNAIDVLEKPKVIDYILSLFDPERGSFSGDSWGETDARFSYCAVSGLALLGGLERLDRERVVGFISRCQNFDGGFGMVEGAESHAAYVWTCVGTLAILGRLDMVDKNTLGWWLSERQVPNGGLNGRPEKLEDVCYSWWALASLVIIGKAEWIDRSKLTSFILSCQDPDSGGIADRPGDMPDVWHTVFGLAGLSMLGYEGLKPVDPVFCMPAQFTKGLVEENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GAR9</accession>
    <name>A0A180GAR9_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Imidazoleglycerol-phosphate dehydratase</fullName>
            <ecNumber evidence="4 9">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PTTG_07833</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="4 9"/>
    <dbReference type="EMBL" id="ADAS02000117">
        <property type="protein sequence ID" value="OAV89826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GAR9"/>
    <dbReference type="EnsemblFungi" id="PTTG_07833-t43_1">
        <property type="protein sequence ID" value="PTTG_07833-t43_1-p1"/>
        <property type="gene ID" value="PTTG_07833"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_07833"/>
    <dbReference type="OrthoDB" id="447729at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000001">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="entry name" value="Imidazole glycerol phosphate dehydratase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133:SF2">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV89826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_07833-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="152" mass="16513" checksum="0DA560ADE2B0D508" modified="2016-09-07" version="1">MSLRLNCKGDLHIDDHHTTEDVAIALGQAFHQALFGQNGEGLKGIRRFGTGFAPLDEALARAVVDISNRPTCSTDLKLKREMIGNLSCEMIPHVFYSFAMGAQITLHVDVLKGENDHHKSEASFKALALALKQAIERTGSDEVPSTKGTLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GAT9</accession>
    <name>A0A180GAT9_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Kinesin motor domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_05251</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000122">
        <property type="protein sequence ID" value="OAV89629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="ADAS02000122">
        <property type="protein sequence ID" value="OAV89630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_05251-t43_2">
        <property type="protein sequence ID" value="PTTG_05251-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05251"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_05251-t43_3">
        <property type="protein sequence ID" value="PTTG_05251-t43_3-p1"/>
        <property type="gene ID" value="PTTG_05251"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05251"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
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                <person name="Zeng Q."/>
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                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
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                <person name="Goldberg J."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
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                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Khalil H.B."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
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                <person name="Larson L."/>
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                <person name="Pearson M."/>
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                <person name="Dewhirst F.E."/>
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                <person name="Nusbaum C."/>
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        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000115">
        <property type="protein sequence ID" value="OAV89905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GB42"/>
    <dbReference type="EnsemblFungi" id="PTTG_05628-t43_2">
        <property type="protein sequence ID" value="PTTG_05628-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05628"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05628"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045154">
        <property type="entry name" value="PCF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054127">
        <property type="entry name" value="Pcf11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921:SF3">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX 2 PROTEIN PCF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21936">
        <property type="entry name" value="Pcf11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pcf11 C-terminal" evidence="2">
        <location>
            <begin position="414"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="565"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="249"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="513"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="592"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV89905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05628-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="612" mass="67778" checksum="2A18832A938DA16F" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GB65</accession>
    <name>A0A180GB65_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidinol-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_03242</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-histidinol phosphate + H2O = L-histidinol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14465"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="3.1.3.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 8/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PHP hydrolase family. HisK subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.15" evidence="3"/>
    <dbReference type="EMBL" id="ADAS02000120">
        <property type="protein sequence ID" value="OAV89708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GB65"/>
    <dbReference type="EnsemblFungi" id="PTTG_03242-t43_2">
        <property type="protein sequence ID" value="PTTG_03242-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03242"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03242"/>
    <dbReference type="OrthoDB" id="2496328at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00013"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004401">
        <property type="term" value="F:histidinol-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd12110">
        <property type="entry name" value="PHP_HisPPase_Hisj_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010140">
        <property type="entry name" value="Histidinol_P_phosphatase_HisJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01856">
        <property type="entry name" value="hisJ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21039">
        <property type="entry name" value="HISTIDINOL PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21039:SF0">
        <property type="entry name" value="HISTIDINOL-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHP" evidence="8">
        <location>
            <begin position="5"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OAV89708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03242-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="426" mass="48481" checksum="61C710FE1A733FA4" modified="2016-09-07" version="1">MPVSLHSHSGQFCKHATGTLDSVIRTAIDRGFISYGLSEHMPRDRVQDLYPEESEIQPCELSEQFDRFIAEAGRLKQLYSSKIGLLVGVETELIEPSNTFELLDRTLARHRDILDYLVGSVHHVNQIPIDFDRPTFDQAVLSFDSKNHSLEPVYQLCEAYFDSQYMLINKYRPEVIGHFDLCLLFNPDLDLQAAPAVWTKAERNIRLAISYGALFEVNSASIRKGWSTPYPSPPILQLIIALDGRLTLSDDSHGSDRVGLNYDKSFDYLKAHNVPTLWYLIPASEDNPILQPRGKVSAVRIPGLWWQHPFWSHVTSPNYVTATSASDHFEGLSQHTSSSCSSMPIELASLNFQPSQDTNKNPKGERFKWSQFPIDRFTNQTWFIVSSSLPFWKNKKNATISYSNLDNEASSVMYDQFGRVSRIPDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GB74</accession>
    <name>A0A180GB74_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-histidine N-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PTTG_28556</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblFungi" id="PTTG_28556-t43_1">
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        <property type="gene ID" value="PTTG_28556"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_28556"/>
    <dbReference type="OrthoDB" id="2506646at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Methyltransf_16"/>
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    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR14614:SF39">
        <property type="entry name" value="HISTIDINE PROTEIN METHYLTRANSFERASE 1 HOMOLOG"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038126"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="OAV89779.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_28556-t43_1-p1"/>
        </source>
    </evidence>
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    </evidence>
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    <name>A0A180GC48_PUCT1</name>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000127">
        <property type="protein sequence ID" value="OAV89473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="PTTG_28684-t43_1-p1"/>
        <property type="gene ID" value="PTTG_28684"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_28684"/>
    <dbReference type="OrthoDB" id="2502406at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV89473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_28684-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="142" mass="16012" checksum="4259CD46C28C6DED" modified="2016-09-07" version="1">MKSDFKNLAHRVRDLMLTEANISQTLSNLVETSHSNERELTNEIIDDFGETLEQAAKVNANIHELQRRIKVLNGRMKKKKEEFINELASQKAKNAEEMAEFKEQLSIEAASLPAKIQAAKSTKAEQKQVQLKLAKILADGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GC71</accession>
    <name>A0A180GC71_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">MC family mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PTTG_08811</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="16">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000126">
        <property type="protein sequence ID" value="OAV89503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GC71"/>
    <dbReference type="EnsemblFungi" id="PTTG_08811-t43_2">
        <property type="protein sequence ID" value="PTTG_08811-t43_2-p1"/>
        <property type="gene ID" value="PTTG_08811"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08811"/>
    <dbReference type="OrthoDB" id="2503921at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000131">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="63"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="160"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="261"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OAV89503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08811-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="38361" checksum="243B245898D4EA24" modified="2016-09-07" version="1">MSADIVDDTKSKGKQQVSKSQGGIDDAKAQISSEVEYLRSKVAPAFSDIGGASKSSPELHSSKYYAACISAGVAACGLTHALVTPLDLVKCRRQVDKALYKGNFDGWSKIYNSGGLSALYTGVGPTWIGYSVQGGCKYGFYEYLKKKYADAVGPVNAVKYKDAIYLAGSASAELIADAAYVPLEAVKVRMQTTIPPFASGTVDGFKKFTAVEGIGGLYKSLGSLWTRQIPYTMMKFWSFEATVTKIYASLGKPKDSYNKLQQLGVSFVGGYFAGIFCAVVSHPADTMVSKLNAVGKEGSSKPTVRSIYNEIGFGGLWAGLGTRIMMIGTLTALQWLIYDYVKVSFGFPTTGSAEPIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GCY4</accession>
    <name>A0A180GCY4_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DYNc domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_11953</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000100">
        <property type="protein sequence ID" value="OAV90520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GCY4"/>
    <dbReference type="EnsemblFungi" id="PTTG_11953-t43_2">
        <property type="protein sequence ID" value="PTTG_11953-t43_2-p1"/>
        <property type="gene ID" value="PTTG_11953"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_11953"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF235">
        <property type="entry name" value="DYNAMIN-RELATED PROTEIN DNM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin GTPase" evidence="1">
        <location>
            <begin position="38"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV90520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11953-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="768" mass="86623" checksum="9C42D83631812672" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GDY5</accession>
    <name>A0A180GDY5_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zf-LYAR domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_08116</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
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            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="OAV90542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblFungi" id="PTTG_08116-t43_2">
        <property type="protein sequence ID" value="PTTG_08116-t43_2-p1"/>
        <property type="gene ID" value="PTTG_08116"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08116"/>
    <dbReference type="OrthoDB" id="21474at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="LYAR"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_LYAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13100:SF10">
        <property type="entry name" value="CELL GROWTH-REGULATING NUCLEOLAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CELL GROWTH-REGULATING NUCLEOLAR PROTEIN LYAR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-LYAR"/>
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    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
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        <location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="7">
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            <end position="384"/>
        </location>
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    </evidence>
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</entry>
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            <taxon>Pucciniomycetes</taxon>
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    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000096">
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    <dbReference type="PANTHER" id="PTHR35204">
        <property type="entry name" value="YALI0A21131P"/>
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        <source>
            <dbReference type="EMBL" id="OAV90668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09733-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GE91</accession>
    <name>A0A180GE91_PUCT1</name>
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        <recommendedName>
            <fullName evidence="3 9">Chromatin modification-related protein EAF6</fullName>
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    <gene>
        <name evidence="12" type="ORF">PTTG_09162</name>
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    <organism evidence="12">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
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                <person name="Thomson T."/>
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                <person name="White J."/>
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                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the EAF6 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000088">
        <property type="protein sequence ID" value="OAV91046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GE91"/>
    <dbReference type="EnsemblFungi" id="PTTG_09162-t43_2">
        <property type="protein sequence ID" value="PTTG_09162-t43_2-p1"/>
        <property type="gene ID" value="PTTG_09162"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09162"/>
    <dbReference type="OrthoDB" id="440324at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015418">
        <property type="entry name" value="Eaf6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13476">
        <property type="entry name" value="CHROMATIN MODIFICATION-RELATED PROTEIN MEAF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09340">
        <property type="entry name" value="NuA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="154"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OAV91046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09162-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="187" mass="19880" checksum="B51C76602162D775" modified="2016-09-07" version="1">MADSAAPDPPTQEVSGQAEQSNPAGETGPNGTSAPKGDVDPGVLSRLESAKIELAMNLAKKKKLDKELAALEATLYSHETAYLTDPSANLFGNIVKGYEAYVKAPPSTSMAGDHHSTRRKRAYTNTIGPAGPTEMVGDAERIFSKSSGSYARALEVRSKEHESAVTSEQESVVPTLHPPDSRKKSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GEZ0</accession>
    <name>A0A180GEZ0_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP-dependent RNA helicase DBP8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PTTG_06798</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000083">
        <property type="protein sequence ID" value="OAV91287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GEZ0"/>
    <dbReference type="EnsemblFungi" id="PTTG_06798-t43_1">
        <property type="protein sequence ID" value="PTTG_06798-t43_1-p1"/>
        <property type="gene ID" value="PTTG_06798"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06798"/>
    <dbReference type="OrthoDB" id="10261904at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF24">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="179"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV91287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06798-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="43154" checksum="B3C877806543A7D8" modified="2016-09-07" version="1">MDMMSQALDLNKRPHIIIATPGRLVDHLKSAEGEAGRWNLRRCKFLVLDEADRMLTPTFAESLSYLINEQLPPATDRQTLLFTATITEPVLALSEKTPPAGKQKPVVHLCGPSRINIPPSLKQFYAFIPSQVKDVYLYFLLLNVCDLTGLPPPPKRQKELKSKRVKYSNKAKRLINEDDETDPVSNLPQTIIFVSKPRVAEQLHLLLDSSHLPYPIKSRSLHSNLSQMQRLDAIREFEERQVPVLISTDLGSRGLDLKQVKLVINWNVPLDPNEYVHRVGRTARRNQGGDGHGVSISLVAEHDVECVLKVEEQIGSKLELIQLKEEKVLEYLNPISKAKREAELDLNEMKFGQRQLINKKKQQKLDKIRRASQDQSTHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GGA3</accession>
    <name>A0A180GGA3_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nfu_N domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_05101</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000080">
        <property type="protein sequence ID" value="OAV91458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GGA3"/>
    <dbReference type="EnsemblFungi" id="PTTG_05101-t43_2">
        <property type="protein sequence ID" value="PTTG_05101-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05101"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05101"/>
    <dbReference type="OrthoDB" id="565552at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000001">
        <property type="entry name" value="NFU1 iron-sulfur cluster scaffold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.70">
        <property type="entry name" value="Scaffold protein Nfu/NifU, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014824">
        <property type="entry name" value="Nfu/NifU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036498">
        <property type="entry name" value="Nfu/NifU_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001075">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178">
        <property type="entry name" value="IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178:SF1">
        <property type="entry name" value="NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08712">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01106">
        <property type="entry name" value="NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00932">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110836">
        <property type="entry name" value="Hypothetical protein SAV1430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffold protein Nfu/NifU N-terminal" evidence="2">
        <location>
            <begin position="71"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV91458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05101-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="287" mass="31987" checksum="025E561FD4373CC5" modified="2016-09-07" version="1">MPLLKNCVERCSPSIYNLTASTLKNSPRIILSNRLPNRSYHSYSLPGQLRIKELNRASPQYLNATKKPTILRHEPTPNADALKFIPGRPVMKSGSREFLVGDDTRSSPLARSLLSVEGVRSVFFGPDFISINKESGLGWPTMKPEIYSLLMEFFSASDRPVVQEGPIEEDSGPLDTRINDDDSEVVAMIKELLDTRVRPAIQEDGGDLEYKGFNEETGVVQLMLKGSCRGCDSSAVTLKSGIERMLMHYVPEVQCVEQVQSEEEKMAEDEFAKFEARLRGGGKTVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GIA0</accession>
    <name>A0A180GIA0_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PPM-type phosphatase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_06531</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000077">
        <property type="protein sequence ID" value="OAV91693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GIA0"/>
    <dbReference type="EnsemblFungi" id="PTTG_06531-t43_2">
        <property type="protein sequence ID" value="PTTG_06531-t43_2-p1"/>
        <property type="gene ID" value="PTTG_06531"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06531"/>
    <dbReference type="OrthoDB" id="420076at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004741">
        <property type="term" value="F:[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF792">
        <property type="entry name" value="GM14286P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="52"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV91693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06531-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="542" mass="59670" checksum="D8D4D27AD3FF00C9" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GID0</accession>
    <name>A0A180GID0_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_02235</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
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                <person name="Khalil H."/>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
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                <person name="Sakthikumar S."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
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                <person name="Wang M."/>
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                <person name="Hulbert S."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
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                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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                <person name="Khalil H.B."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
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                <person name="Zeng Q."/>
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    <name>A0A180GKE6_PUCT1</name>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
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                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
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                <person name="Tanner A.C."/>
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                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
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    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
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        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <reference evidence="4" key="4">
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                <consortium name="EnsemblFungi"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
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        <property type="gene ID" value="PTTG_02711"/>
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    <dbReference type="OrthoDB" id="3231855at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF1771"/>
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    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
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        <property type="entry name" value="Smr_dom_sf"/>
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        <property type="entry name" value="Smr_domain_protein"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47417:SF1">
        <property type="entry name" value="SMR DOMAIN-CONTAINING PROTEIN YPL199C"/>
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        <property type="entry name" value="SMR"/>
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        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
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                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
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                <person name="Shenoy N."/>
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                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
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                <person name="Dewhirst F.E."/>
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                <person name="Nusbaum C."/>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
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    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000052">
        <property type="protein sequence ID" value="OAV93266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblFungi" id="PTTG_08555-t43_1">
        <property type="protein sequence ID" value="PTTG_08555-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08555"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08555"/>
    <dbReference type="OrthoDB" id="2506497at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033557">
        <property type="term" value="C:Slx1-Slx4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048749">
        <property type="entry name" value="SLX1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050381">
        <property type="entry name" value="SLX1_endonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20208">
        <property type="entry name" value="STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20208:SF10">
        <property type="entry name" value="STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21202">
        <property type="entry name" value="SLX1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Structure-specific endonuclease subunit SLX1 C-terminal" evidence="2">
        <location>
            <begin position="182"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV93266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08555-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="396" mass="44766" checksum="0748276F63E6146B" modified="2016-09-07" version="1">MDFQFEWAWNTPYKSRHLQVVKTIKDDPKQTELDSAGAIAGKATATATKTKKPMFPRSVGNRVEIKLKVLRKMMTTLPWSQYPLKVLFFEESAYQLWLEQAKPPKSAAKQTSGTTDTLEMNVHPIEAAFRPEGVDGTRKDRHAIPSTPYDQRKPIEVHDEEAGLEDYEKIELIKKRCNSDLRCSLCEQSIDIQDHLSYLICRSPDCLMSAHLLCLSKHFLDASLIDCSPLLTIGDEPQPSLPRVLPDRGRCPTCSVDLRWGELIKGCYRRLPKNHTKAQISDTDDEEDADVAFPYADEDNQYTHGNTSVTEDDHELNVCGSVTSSRGSKTKQTKPKQMSSRKPATSGKQPNSNSANKGRYAKPKASKRTHKQIPEEDEDEPEQDFVKLMDELQVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GKH3</accession>
    <name>A0A180GKH3_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PTTG_02691</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="19">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 20">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 20">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4"/>
    <dbReference type="EMBL" id="ADAS02000057">
        <property type="protein sequence ID" value="OAV92959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GKH3"/>
    <dbReference type="EnsemblFungi" id="PTTG_02691-t43_2">
        <property type="protein sequence ID" value="PTTG_02691-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02691"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02691"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000012">
        <property type="entry name" value="Protein O-mannosyl-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF46">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="200"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="237"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="344"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="410"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="477"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OAV92959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02691-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="542" mass="61507" checksum="C7D64ADB21072417" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GKI3</accession>
    <name>A0A180GKI3_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_11969</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
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                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
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                <person name="Gellesch M."/>
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                <person name="Griggs A."/>
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                <person name="Heilman E."/>
                <person name="Heiman D."/>
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                <person name="Howarth C."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">1-1 BBBD Race 1</strain>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
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                <person name="Adiconis X."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
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            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
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                <person name="Feldgarden M."/>
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                <person name="Zeng Q."/>
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                <person name="Bochicchio J."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
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                <person name="Joly D."/>
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                <person name="Sakthikumar S."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
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            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
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        <property type="protein sequence ID" value="PTTG_27267-t43_1-p1"/>
        <property type="gene ID" value="PTTG_27267"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_27267"/>
    <dbReference type="OrthoDB" id="165352at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.20">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494:SF1">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="223"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="438"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="454"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV93580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_27267-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="492" mass="54127" checksum="3F385020047BF5AD" modified="2016-09-07" version="1">MSTDRLLNLATAPRSGPSEPGTQNGANDRLLSGESSPSTIRSRFRAGVQAVTAAQRVVSGPSNPTFHSSPTTNDSSQHRYQWSHTGPLEPGINPNHPQPQFAHLSNQPVSVDVVDYSDAQVRFQSFEPALPSELHRLERAVGAHTRPFTAQAAPQEETSRPPWAAVRWINVNGLSWEVIKLLAIQYQLHPLAVEDCLHTSARSKSEYYKQHLFVHAAVHTLEERSEWPDSPPPAEKNPSKPGPASDRPLESGTMDPEIDPVGSAPSLYKSSDYTHEPSDQPIMDLRTATNGPRVPGKEVFDAEREVVRQLTKDYKVRIHVEKLSIFLLRDGTLITIFSHDGSKSVLPLLARLKSMDTLLRSSADGSMLLQAVLDIVVDQALDVVEAFRSKLTGLEAKVLVSPDLDTVRHLHVLSGELLLLKRTLNPLSTLVHGIMNHDPSERRATNYRGTQRSSESERGGGEDVRSLEPQSSTFCTGFLSSQAKAVSVDLYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GMI0</accession>
    <name>A0A180GMI0_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="14">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PTTG_06724</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="18">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="14"/>
    <dbReference type="EMBL" id="ADAS02000044">
        <property type="protein sequence ID" value="OAV94017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GMI0"/>
    <dbReference type="EnsemblFungi" id="PTTG_06724-t43_2">
        <property type="protein sequence ID" value="PTTG_06724-t43_2-p1"/>
        <property type="gene ID" value="PTTG_06724"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06724"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23285">
        <property type="entry name" value="beta-trefoil_MIR_PMT4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000044">
        <property type="entry name" value="Dolichyl-phosphate-mannose-protein mannosyltransferase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF51">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="253"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="628"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="666"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="699"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="746"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="355"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="424"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="29"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OAV94017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06724-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="793" mass="90997" checksum="2AC463F5A36A127D" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GNJ4</accession>
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        <name type="common">Brown leaf rust fungus</name>
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            <taxon>Pucciniomycetes</taxon>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000044">
        <property type="protein sequence ID" value="OAV94038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GNJ4"/>
    <dbReference type="EnsemblFungi" id="PTTG_27128-t43_1">
        <property type="protein sequence ID" value="PTTG_27128-t43_1-p1"/>
        <property type="gene ID" value="PTTG_27128"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_27128"/>
    <dbReference type="OrthoDB" id="2495629at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV94038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_27128-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="163" mass="19208" checksum="185DFEC36302B587" modified="2016-09-07" version="1">MAIDWSKEEIIQLRAVLLTQIDILADWDWNHAKQDLKIIADILELKVEPYEEPSPLILSSGLMTFEPSRSAQAQSRLPRFDQADQTRRRRRPKVDYDPSILAAAQFLFGHIPAFNSHLDKNIFSLETDQPVLFKLITEFSKVYRWSQFYLSYHQRKKKRNGNF</sequence>
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<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
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    <name>A0A180GNM4_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000039">
        <property type="protein sequence ID" value="OAV94407.1"/>
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        <property type="gene ID" value="PTTG_02213"/>
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    <dbReference type="OrthoDB" id="2500831at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
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    <proteinExistence type="predicted"/>
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            <end position="538"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="417"/>
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        <location>
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        </location>
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    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
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            <end position="479"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV94407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02213-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="538" mass="61088" checksum="53C395564EE43847" modified="2016-09-07" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GNM8</accession>
    <name>A0A180GNM8_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Adenosinetriphosphatase</fullName>
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    <gene>
        <name evidence="7" type="ORF">PTTG_06725</name>
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    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
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    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000044">
        <property type="protein sequence ID" value="OAV94019.1"/>
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        <property type="gene ID" value="PTTG_06725"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06725"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0016787">
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                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
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    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV94428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02203-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="519" mass="57512" checksum="8E64A52442EAB8B9" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GPR3</accession>
    <name>A0A180GPR3_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_02531</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000041">
        <property type="protein sequence ID" value="OAV94292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GPR3"/>
    <dbReference type="EnsemblFungi" id="PTTG_02531-t43_2">
        <property type="protein sequence ID" value="PTTG_02531-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02531"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02531"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008110139" evidence="1">
        <location>
            <begin position="22"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV94292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02531-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="130" mass="14354" checksum="A574A66CEFDDA4B1" modified="2016-09-07" version="1">MLRLSLVAIFLSLVALNAVYASQEEPHALAKRGSTWHVCASFHQKRRCLHGDNACCHVVCSHPMRHSWWIALKDKCDALHHKPKRAKTPPPVNPPQTIVVTAPPPPTAYLVQPQPAVVYAQPAQVPAQAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GPR9</accession>
    <name>A0A180GPR9_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peroxin-5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_03023</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxisomal targeting signal receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000039">
        <property type="protein sequence ID" value="OAV94514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GPR9"/>
    <dbReference type="EnsemblFungi" id="PTTG_03023-t43_2">
        <property type="protein sequence ID" value="PTTG_03023-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03023"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03023"/>
    <dbReference type="OrthoDB" id="10006023at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005052">
        <property type="term" value="F:peroxisome matrix targeting signal-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024111">
        <property type="entry name" value="PEX5/PEX5L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR PEX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130:SF9">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="562"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="689"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="723"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="290"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="635"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="292"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="648"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OAV94514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03023-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="871" mass="96126" checksum="7489C5361B76986E" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GPS7</accession>
    <name>A0A180GPS7_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mak10 subunit, NatC N(Alpha)-terminal acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PTTG_03096</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
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                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
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                <person name="Bruce M."/>
                <person name="Wang M."/>
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                <person name="Hulbert S."/>
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                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000039">
        <property type="protein sequence ID" value="OAV94412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GQ50"/>
    <dbReference type="EnsemblFungi" id="PTTG_11916-t43_2">
        <property type="protein sequence ID" value="PTTG_11916-t43_2-p1"/>
        <property type="gene ID" value="PTTG_11916"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_11916"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008110163" evidence="2">
        <location>
            <begin position="28"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV94412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11916-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="275" mass="31053" checksum="061D6C8B2B206744" modified="2016-09-07" version="1">MRFQDYHHSCLSLQLLVICGLKLAVIGRPTFTNDIHLIPEQHTITDVPKAGVLHKKMNSVLEKVSPTSKKSIPTPGRPGFQRALDGADLIEHLYKVVATELLKNEMKPLPADDGEINTIDELEKLHGTVHELLSDADLAAADSLPAGKIEIMMSRFREKIRPQLMRYFSENKDPSNDLHVQIQQALEQRDPREETPNLRKKLNRSEALTRKKVVEAFKNDIEQGLMSDTEEDSKTLGTERRKKNISTAIFSVFRKTKPDPQQNPASSSNVGRFNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GQP0</accession>
    <name>A0A180GQP0_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell division cycle protein 123</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_03807</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDC123 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000038">
        <property type="protein sequence ID" value="OAV94592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GQP0"/>
    <dbReference type="EnsemblFungi" id="PTTG_03807-t43_2">
        <property type="protein sequence ID" value="PTTG_03807-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03807"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03807"/>
    <dbReference type="OrthoDB" id="360540at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009772">
        <property type="entry name" value="CDC123"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323:SF6">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 123 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323">
        <property type="entry name" value="D123 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07065">
        <property type="entry name" value="D123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV94592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03807-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="226" mass="25701" checksum="571D56500F8FBBEF" modified="2016-09-07" version="1">MDVYILLKGSDCVFHDLDLHDKIPQAPPEDPLPAVLVLREWFNLNPSHEFRCFVRGRKLIAISARSSTFYDFLLPQGVRKSILERIRAFFEAVICPEFHLSDFIFDVYLKNPTKLAPPSSTSKPNLKLVDINPFATYIDPCLFSYEELEQCCSNASGAAGEQKQSTQLHQTTPLLKLVESEDPHQNFRASKFQTSKLPAEFVNFSQDQGVDQLSDGFKKMLDAEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GQP5</accession>
    <name>A0A180GQP5_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_26841</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000035">
        <property type="protein sequence ID" value="OAV94844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GQP5"/>
    <dbReference type="EnsemblFungi" id="PTTG_26841-t43_1">
        <property type="protein sequence ID" value="PTTG_26841-t43_1-p1"/>
        <property type="gene ID" value="PTTG_26841"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_26841"/>
    <dbReference type="OrthoDB" id="10634002at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV94844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
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                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
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                <person name="Goldberg J."/>
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                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
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                <person name="Zeng Q."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
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                <person name="Zeng Q."/>
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                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
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                <person name="Wang M."/>
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                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
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                <person name="Howarth C."/>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000035">
        <property type="protein sequence ID" value="OAV94874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GQS5"/>
    <dbReference type="EnsemblFungi" id="PTTG_00776-t43_2">
        <property type="protein sequence ID" value="PTTG_00776-t43_2-p1"/>
        <property type="gene ID" value="PTTG_00776"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00776"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="143"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV94874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00776-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="326" mass="35813" checksum="356DDD7AA7562D0A" modified="2016-09-07" version="1">MTTNNNEANPPPLRQSVARVSRPFTAHTATLPSHQSTSRLPLLPTARVEASPAACMYLSKASIHGPKLIKPLRAHTATLVNDAIWIFGGTDLNGCFKDVWKLDLETLSFARMKCKGLEEEMDEDLNLGSSSLDDSLDPHYNLRNSSTNNLSPARRLRSNPKDHIPPPCRAHSATHLDGRIFIFGGGDGPNYFDVLYYLDTISLTWTKPRVKGVLPSTRRAHATVLYGTQLIIFGGGNGSRALNDVHALDLSDLSNLEWRELAIKGRSPLNRGYHSANLVGSKCIIFGGSDGGECFSDIFILDLENLTWIQVEVECPIARLAHTSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GQS8</accession>
    <name>A0A180GQS8_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_12265</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000038">
        <property type="protein sequence ID" value="OAV94632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GQS8"/>
    <dbReference type="EnsemblFungi" id="PTTG_12265-t43_1">
        <property type="protein sequence ID" value="PTTG_12265-t43_1-p1"/>
        <property type="gene ID" value="PTTG_12265"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12265"/>
    <dbReference type="OrthoDB" id="2506847at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="208"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV94632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12265-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="402" mass="44868" checksum="157CBA01B71A986E" modified="2016-09-07" version="1" fragment="single">MRESMPFFYSLLMYKLTGTSNKTTGPNLPSEEDDCLSDASSDSESEESSSYSPAKRNKRCHSVATTICSAVSFISNRRRNGLQLANAVVMLACGVSERVGSYLHYIGLSASRKSAQRALQSLGRHAQQAIKKAAHVVEPVNFAPIICIDNVDFEEHIHSKSAERQSRMFHGTWGYLHRFDPVLLKTVDPEDLSLPAYKAAIAQSADMVVRPSLFLPTNEENIHFREVLKSQITTVLLRYIATPNDKDHLLPLVPPVIDQINPCVPDITTLKLMLASDNSAEGIGEVFESITKQLKLEKSDFFGRLQVFEGDLGTCLNIESLRAQRKPSRHVEDAFSNCFTLLGASHILWNFAQRLLLYHFGDNTDANDLGAWHCLEALNIPSKRPTAKNDFTLMIAHIQKVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GR11</accession>
    <name>A0A180GR11_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase family 32 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_12301</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000033">
        <property type="protein sequence ID" value="OAV94991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GR11"/>
    <dbReference type="EnsemblFungi" id="PTTG_12301-t43_1">
        <property type="protein sequence ID" value="PTTG_12301-t43_1-p1"/>
        <property type="gene ID" value="PTTG_12301"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12301"/>
    <dbReference type="OrthoDB" id="3647at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051999">
        <property type="term" value="P:mannosyl-inositol phosphorylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.20:FF:000005">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.17, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051706">
        <property type="entry name" value="Glycosyltransferase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32385:SF15">
        <property type="entry name" value="INOSITOL PHOSPHOCERAMIDE MANNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32385">
        <property type="entry name" value="MANNOSYL PHOSPHORYLINOSITOL CERAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="558"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="595"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="666"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="772"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="124"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="178"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OAV94991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12301-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="803" mass="89156" checksum="E8E23EE2535B6696" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GR12</accession>
    <name>A0A180GR12_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_26694</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000030">
        <property type="protein sequence ID" value="OAV95267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GR12"/>
    <dbReference type="EnsemblFungi" id="PTTG_26694-t43_1">
        <property type="protein sequence ID" value="PTTG_26694-t43_1-p1"/>
        <property type="gene ID" value="PTTG_26694"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_26694"/>
    <dbReference type="OrthoDB" id="2519139at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV95267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_26694-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="351" mass="38245" checksum="DA220B6264BED16D" modified="2016-09-07" version="1">MTGPANFTTEARATDRETRTTCLARHGPTDSDKLATPVRSERNSANNMKSEKQLIRIANSTLPQDRVDFRGRVEIVYDSSTDITVNDIIEDNVTFGIIPCHGVNAGGPQELQVLIRLQHFEGKNPAHGHVYDLKGSVVGYRDRYTPHLSCSSAVMFQYQPTNTTTCAGSLNINAVGTITSVREKATRGASGEVSYKLTVRHECNHNILSIVYIVPRPALQLPFSLERYEGCRVWLLGIVNGVDELECMVVVNGEFKLESNSTTVQRGLAAQSCAKVAIKLSKAFDQRLTATPTPTKSNRVAIVLFVLVAVVSLVQAPHTAAPCIFTGLQGDSPTPYKLQQSCRDQGDPGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GR20</accession>
    <name>A0A180GR20_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="17">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PTTG_04379</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="22 23" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="22">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="22" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 17">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="17"/>
    <dbReference type="EMBL" id="ADAS02000031">
        <property type="protein sequence ID" value="OAV95200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GR20"/>
    <dbReference type="EnsemblFungi" id="PTTG_04379-t43_1">
        <property type="protein sequence ID" value="PTTG_04379-t43_1-p1"/>
        <property type="gene ID" value="PTTG_04379"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04379"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
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                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
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                <person name="Nusbaum C."/>
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            <strain evidence="4">1-1 BBBD Race 1</strain>
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                <person name="Szabo L."/>
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                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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                <consortium name="EnsemblFungi"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
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                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
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                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
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                <person name="Izard J."/>
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                <person name="Dewhirst F.E."/>
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                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <reference evidence="3" key="4">
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
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    </evidence>
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            <dbReference type="EMBL" id="OAV94703.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblFungi" id="PTTG_26908-t43_1-p1"/>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GRW1</accession>
    <name>A0A180GRW1_PUCT1</name>
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        <submittedName>
            <fullName evidence="17">CFEM domain-containing protein</fullName>
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    </protein>
    <gene>
        <name evidence="16" type="ORF">PTTG_08490</name>
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    <organism evidence="16">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
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        </lineage>
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    <reference evidence="16" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
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                <person name="Baranova O.V."/>
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                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="17">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
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            <topology evidence="1">GPI-anchor</topology>
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        <property type="gene ID" value="PTTG_08490"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08490"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="CFEM_domain"/>
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    <dbReference type="InterPro" id="IPR008427">
        <property type="entry name" value="Extracellular_membr_CFEM_dom"/>
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    <dbReference type="PANTHER" id="PTHR37928">
        <property type="entry name" value="CFEM DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G14090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37928:SF2">
        <property type="entry name" value="GPI ANCHORED CFEM DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G10580)"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF05730">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00747">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52012">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
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    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
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        <location>
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            <end position="181"/>
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    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="24"/>
            <end position="57"/>
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    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010031"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS52012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OAV95567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08490-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="215" mass="21511" checksum="07AC65B4D45D35E6" modified="2016-09-07" version="1">MGASLLGEVLRYRALRVAGCAHPAADSPARSGHLHTPEHPTKTNSPPPTPNPPPPSTTMLTIQAITAHAILALACLALACLAPAASAQDTSGLPACAQNCLAASLRSSARSCAPTDFACLCRNSEFVAASDSCYSTSCSAGELAAAQEWGVKSCAAAGIQITLNGANNTSTGTNTVPLNNTSTPARPNAAPALSAEAGKLLAMGTACLSSFLIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GS39</accession>
    <name>A0A180GS39_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Inositol-3-phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_26617</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000027">
        <property type="protein sequence ID" value="OAV95646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GS39"/>
    <dbReference type="EnsemblFungi" id="PTTG_26617-t43_2">
        <property type="protein sequence ID" value="PTTG_26617-t43_2-p1"/>
        <property type="gene ID" value="PTTG_26617"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_26617"/>
    <dbReference type="OrthoDB" id="2887at2759"/>
    <dbReference type="UniPathway" id="UPA00823">
        <property type="reaction ID" value="UER00787"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2360.10">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11510">
        <property type="entry name" value="MYO-INOSITOL-1 PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07994">
        <property type="entry name" value="NAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008110228" evidence="2">
        <location>
            <begin position="31"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV95646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_26617-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="357" mass="39575" checksum="A1941D855D61A273" modified="2016-09-07" version="1">MSRVARMFIPRYLTCYQWLVLILCSAEISAGCPSTRLHRAKVMEWDLQDQLNPLMSQMKPLPNVCYPDFINARQEERADNVLPGSNKQAHSSRRPRNSTKSLYSATNTEKIIPGVNDTAPNMEQAVRNNHDGIYPSTLLAMACIYESAPYVNGAPQNTFVPGCAELAEELGRFIGGHDFKSGQTKIKSCLAEFLVNSGIKPLSIASYNHLVSSSLLSNTTASWRCEYLRSSTFLFSFNIYSSGLLDRGGRNKIVISNVTCQDSLLAIPLILDLVIITKLLTRVQYRKPSGNATEESDVQKLYPVLSLLSSMLKASLVRPGPDVINGAARQRAAIDHFLMALLGLQPLTEWEQSKIMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GS42</accession>
    <name>A0A180GS42_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="16">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PTTG_04379</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="21 22" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="22">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="21" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 16">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="16"/>
    <dbReference type="EMBL" id="ADAS02000031">
        <property type="protein sequence ID" value="OAV95199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GS42"/>
    <dbReference type="EnsemblFungi" id="PTTG_04379-t43_2">
        <property type="protein sequence ID" value="PTTG_04379-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04379"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04379"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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                <person name="Earl A."/>
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                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
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                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
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                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
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                <person name="Szabo L."/>
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                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
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                <person name="Song X."/>
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                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
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            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
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        <property type="gene ID" value="PTTG_12626"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12626"/>
    <dbReference type="OrthoDB" id="2498586at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV95515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12626-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="368" mass="41809" checksum="38F0613C5F33C506" modified="2016-09-07" version="1" fragment="single">MSFGIKQSLEFAINVFERLTDYDYSRGLVDTPANISVERLRSKNYLLAHLHSTLLPLLRQQIGDLSDVLRARGSLREKPDLVVTLVLEIQPKLELTLDRIIRVINDVIPGPVLEPKQKEDQHLKQFKCYRIFGLVNSIRKEMQSELASFFVDSRRVLERLTLPVERQVAPQNHPHVALQSIDAAIRWAEGSELPIIHNLWQSNLEEVDGALEDLLVVAHPANKGHTQPTIQLAQSFIPLLRLSKLFFKKMAAERIDRQKGTSFTEMSSHQLTLLDKSVDKITQHLYNLVCELEDNADIGPPHAASLSGHVKSIIYPFHSCLFLADLYVVPISTDTKLGLSLIHFKTCDILLRPKKTPDLNQSKIADRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GSU6</accession>
    <name>A0A180GSU6_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_04640</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000028">
        <property type="protein sequence ID" value="OAV95448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GSU6"/>
    <dbReference type="EnsemblFungi" id="PTTG_04640-t43_2">
        <property type="protein sequence ID" value="PTTG_04640-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04640"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04640"/>
    <dbReference type="OrthoDB" id="2505592at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051229">
        <property type="entry name" value="ALYREF_mRNA_export"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965">
        <property type="entry name" value="RNA AND EXPORT FACTOR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965:SF35">
        <property type="entry name" value="RNA ANNEALING PROTEIN YRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="72"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OAV95448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04640-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="153" mass="17012" checksum="A690080B2F4B3216" modified="2016-09-07" version="1">MSTAMNLDRPLDDIISDKRKQHKRSGGVARRGGRTNGTGPVRNGRTPRAQPAVAPVPVAKSLQLPPQMGQGSKIIVSNLPPDVTENQIRELFYTTVGQVTRVTLSYDSRGASKGIAQVEFKRNEDATKAFQQYNKRLIDQSSLFRSFFMSFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GSU8</accession>
    <name>A0A180GSU8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_12299</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000029">
        <property type="protein sequence ID" value="OAV95382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GSU8"/>
    <dbReference type="EnsemblFungi" id="PTTG_12299-t43_2">
        <property type="protein sequence ID" value="PTTG_12299-t43_2-p1"/>
        <property type="gene ID" value="PTTG_12299"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12299"/>
    <dbReference type="OrthoDB" id="2499061at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV95382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12299-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="315" mass="35478" checksum="10423323AC08841E" modified="2016-09-07" version="1">MDVSILLQVALSCGVVLLYSVVGNFFLIYLGFRRSEDPQSEEKEEEVEEEVQSEVSEPSNDALHLLRLTNAFCPPRTEPSIFGRDVNNPRENSYYSEDDDFGEDNFSDVLEESSFCLRDSFEHGHSHRISIKPPAFVHQASVAEITTGDHKSTSSARRSSTTFAPETHHHENEKVNRLFSLITKGTGSHTSQESANRKKSSGFYPNSSCVSEPNGAWGHFADKKKKLFTVRPGINALRRLNQSKQVHQVDQPKLPSHANRIDYMSTVRTRFKLISSKKLSLKPINKAIEPARRVDIHRSSEIDGRRMSSVLFGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GSX6</accession>
    <name>A0A180GSX6_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="12">HSF_DOMAIN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PTTG_05194</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
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    <comment type="subunit">
        <text evidence="7">Homotrimer. Homotrimerization increases the affinity of HSF1 to DNA. Interacts with transcriptional coregulator SSA1 on chromatin.</text>
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            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <text evidence="2 8">Belongs to the HSF family.</text>
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        <property type="protein sequence ID" value="OAV95073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="PTTG_05194-t43_3-p1"/>
        <property type="gene ID" value="PTTG_05194"/>
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    <dbReference type="OrthoDB" id="60033at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
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    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <source>
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            <dbReference type="EMBL" id="OAV95073.1"/>
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        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05194-t43_3-p1"/>
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    </evidence>
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</entry>
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        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
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                <person name="Tanner A.C."/>
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                <person name="Haas B."/>
                <person name="Nusbaum C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
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        <property type="protein sequence ID" value="OAV95845.1"/>
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        <property type="term" value="P:protein folding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Aha1"/>
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        <property type="entry name" value="ASHA1/2-like_C"/>
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        <property type="entry name" value="START-like_dom_sf"/>
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        <property type="entry name" value="HEAT SHOCK PROTEIN 90 HSP90 CO-CHAPERONE AHA-1"/>
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    </dbReference>
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        <property type="entry name" value="LD43819P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09229">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01000">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103111">
        <property type="entry name" value="Activator of Hsp90 ATPase, Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Activator of Hsp90 ATPase AHSA1-like N-terminal" evidence="3">
        <location>
            <begin position="12"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="140"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV95845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12633-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="322" mass="35073" checksum="9E206569FA8A48A6" modified="2016-09-07" version="1">MSNFITNYHWRTKDLSRWAKDWFDEKLLSIGELDGLKVESISTFEGDCELGMRKAKLITIYDLRITVRWSGQGSSGTLTIPEISHDMVESDYVFDGALDSGGKTEVESQAKKSLGPAMCKIFQAFPKAIIDVHGAGFYKENGMSPSETPGGSGTSTPQPTAGNLQTKSAPTAAPVPVDDPSTKTSKGDVVNTSSVRIDGQFMCSASDLFDFLTDESKIPMWSRNPAKMKPEVGAEMSLFGGNVTGKVVSVERPKEFVTTWRAPTWPSGHFGKLKTSLAEGSDSTKLELHLTGVPVGTEEETERNLDIFYLRSLKQIGLGTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GT59</accession>
    <name>A0A180GT59_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Spindle pole body component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PTTG_08489</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000027">
        <property type="protein sequence ID" value="OAV95569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GT59"/>
    <dbReference type="EnsemblFungi" id="PTTG_08489-t43_1">
        <property type="protein sequence ID" value="PTTG_08489-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08489"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08489"/>
    <dbReference type="OrthoDB" id="66546at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF33">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="9">
        <location>
            <begin position="109"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="8">
        <location>
            <begin position="505"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="768"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV95569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08489-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="936" mass="103960" checksum="AD112AF8402DAC67" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GT61</accession>
    <name>A0A180GT61_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_12165</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000032">
        <property type="protein sequence ID" value="OAV95163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GT61"/>
    <dbReference type="EnsemblFungi" id="PTTG_12165-t43_1">
        <property type="protein sequence ID" value="PTTG_12165-t43_1-p1"/>
        <property type="gene ID" value="PTTG_12165"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12165"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV95163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12165-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="427" mass="47851" checksum="8A413BB4CCCE8519" modified="2016-09-07" version="1">MSDLPEDVIAGLVDSFNACRTQDHEEVEHHKNLAIQAVSDLNDKFMGIHNFPTQVNAPPVIFGWVQVRTKLREKLVSFLFPTFSQQVTALAEAVLDLSDSSTEPISKLKLVVEVVSVLGSTMEQLTSCLPFIHPAGLHPRYVSHDKDFKKLKAFITHGLVGRIYEATEDACGLFRSSLAVIEQLGHSLEDQAPSRTKLLTMKASCLESIDQVKQFVDKSEVDIIRDRWQSNIASMSKTLDSFLELASAQSPQDTWSESRTANEDDDEDDTLCEASQVHGPSENEVEKSTIAIVKLGRSGFTKLLRLLSNKHIFTIVTNLPSKELHAFSISADLVSKRIGEMVEILCKGYLTEESRARGMTAMRRSISDLLNAPNVMLPIIKILVPLRAKPDQPSPMIHLKPWFYQWKKHFFLAIQNFLEAGGFDEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GT80</accession>
    <name>A0A180GT80_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_02007</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000026">
        <property type="protein sequence ID" value="OAV95761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GT80"/>
    <dbReference type="EnsemblFungi" id="PTTG_02007-t43_1">
        <property type="protein sequence ID" value="PTTG_02007-t43_1-p1"/>
        <property type="gene ID" value="PTTG_02007"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02007"/>
    <dbReference type="OrthoDB" id="2187549at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070476">
        <property type="term" value="P:rRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039127">
        <property type="entry name" value="Trm112"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773:SF0">
        <property type="entry name" value="MULTIFUNCTIONAL METHYLTRANSFERASE SUBUNIT TRM112-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773">
        <property type="entry name" value="UPF0315 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV95761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02007-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="100" mass="11235" checksum="8E3EBE23B3979FDB" modified="2016-09-07" version="1">MVRLITHNLLACNAKTCSAPTNFPLRFEQVQRVEIKEAELNKEFVKGFLNKLDFEALLYASRALGDAALPDSLPLESLQNPDEIPDEIYAALHHALLEVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GT87</accession>
    <name>A0A180GT87_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_12224</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000026">
        <property type="protein sequence ID" value="OAV95744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GT87"/>
    <dbReference type="EnsemblFungi" id="PTTG_12224-t43_1">
        <property type="protein sequence ID" value="PTTG_12224-t43_1-p1"/>
        <property type="gene ID" value="PTTG_12224"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12224"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF434">
        <property type="entry name" value="GB|AAD18119.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="264"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="215"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV95744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12224-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="325" mass="36367" checksum="95FD824CD2FD8EF6" modified="2016-09-07" version="1">MLVHWLSTKLQTSNKALLTSGKSQSWYRGKTGFWRSTTKTTCPSNAGRPSPTIKNFEAQAANYESGAANHEPGAAHYESVAAHYESVAANYESGPGDGWRRTFMEVGGDWIVVLFDRTSLVNTYIESEGFSNRQRRRLEEAERAFDSHTITSGYIPFNEQQKLHTETRHGTVSLDEASQLLATTAASQPGESDRLADSYDDGGLRFNSKLKGIQHQKTAACSHQDEPTNQDRVTESPNGSSTANPPNHYKYSHFIVGKENQNQCAVCGDQFKSPKNAGKHQWCISEVTRIKKCRHDFHPKCIRPWIIEHGNRSCPLCRVFLDDNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GT96</accession>
    <name>A0A180GT96_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PTTG_08517</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="ADAS02000028">
        <property type="protein sequence ID" value="OAV95522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GT96"/>
    <dbReference type="EnsemblFungi" id="PTTG_08517-t43_1">
        <property type="protein sequence ID" value="PTTG_08517-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08517"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08517"/>
    <dbReference type="OrthoDB" id="2503069at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="46"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="267"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV95522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08517-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="585" mass="65506" checksum="92E0219449DC76E0" modified="2016-09-07" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GYB2</accession>
    <name>A0A180GYB2_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PCI domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_07920</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN7/EIF3M family. CSN7 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000014">
        <property type="protein sequence ID" value="OAV97318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GYB2"/>
    <dbReference type="EnsemblFungi" id="PTTG_07920-t43_1">
        <property type="protein sequence ID" value="PTTG_07920-t43_1-p1"/>
        <property type="gene ID" value="PTTG_07920"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_07920"/>
    <dbReference type="OrthoDB" id="10265275at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045237">
        <property type="entry name" value="COPS7/eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350:SF5">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="38"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="297"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OAV97318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_07920-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="339" mass="37103" checksum="47CA2DE365EFFAD9" modified="2016-09-07" version="1">MGDVHDLDHIKLDLNDHPASSSSAAPAKPAQNPASRIQTAKINHKLEYYLALVGASKGVALTKLVEDLLGSAGIYVFGEILETKPIQELASHPTDASYHELLEIFAYGTWKDYRQRQAHLPKLNDQQLAKLKLLSILTRASHSRIIPYSELMEELEIGETQALEELIIDGIYSGLLGGRLDQKYSRLEVETSVGRDVKLRPLGASSALPKSTQDGDVDMDAADAPGVSHDNTLAKLHAQLADWRDCSARVIEQLDAQISLIRQKDQAKLEHQAAHSATVEALSHSVYSSVMSSSLSGHHKFGRKGKDKDAVDAPLSSDHQMDIDEHDAGIVWEKSSLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GYC4</accession>
    <name>A0A180GYC4_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_08584</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000010">
        <property type="protein sequence ID" value="OAV97825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GYC4"/>
    <dbReference type="EnsemblFungi" id="PTTG_08584-t43_1">
        <property type="protein sequence ID" value="PTTG_08584-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08584"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08584"/>
    <dbReference type="OrthoDB" id="342064at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016070">
        <property type="term" value="P:RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025239">
        <property type="entry name" value="DUF4187"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039727">
        <property type="entry name" value="SE/Ars2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007042">
        <property type="entry name" value="SERRATE/Ars2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021933">
        <property type="entry name" value="SERRATE/Ars2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165">
        <property type="entry name" value="ARSENITE-RESISTANCE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165:SF0">
        <property type="entry name" value="SERRATE RNA EFFECTOR MOLECULE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04959">
        <property type="entry name" value="ARS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12066">
        <property type="entry name" value="SERRATE_Ars2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01173">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="703"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="248"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="529"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="620"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="748"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="925"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="272"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="310"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="562"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="630"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV97825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08584-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="998" mass="109620" checksum="6234B3D665CB426D" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GYE0</accession>
    <name>A0A180GYE0_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_01293</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000012">
        <property type="protein sequence ID" value="OAV97534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GYE0"/>
    <dbReference type="EnsemblFungi" id="PTTG_01293-t43_2">
        <property type="protein sequence ID" value="PTTG_01293-t43_2-p1"/>
        <property type="gene ID" value="PTTG_01293"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_01293"/>
    <dbReference type="OrthoDB" id="2500956at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV97534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_01293-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="350" mass="37871" checksum="0C0FD62F50388211" modified="2016-09-07" version="1">MLFLFTRVGPNGQSSAPETSSSWMLNASSRDLRKAKSTPILRINGTTPTTAGAPSPETTSAGIATDSLGVPSSLTGPALSTSTAVRLACPKPPHISDQAQNKHASFTRKIRYRLSSFNRGPGSPLLQQAATSPTRPDDFSPPVSYSHGSCTMHSSGSMSPTTEKYPSKLYDDAKAVPARESRDRSQSVLEQRSPSYASMTFFKDGLNKAHKFFGRALEGFSDKDSDEDVSDESHDEDEEDLNMLYSNVMVPLEFPGEDDGETTSSSSSPFEPDFDKKVGQTTSPEDSTPSYYFKKQSSSCSLKKNNLLAPQASEPLSASLGSDLYPEMTTTSNPYSITSQQCHDVKLQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GYE8</accession>
    <name>A0A180GYE8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SEC7 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_00126</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000013">
        <property type="protein sequence ID" value="OAV97389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_00126-t43_1">
        <property type="protein sequence ID" value="PTTG_00126-t43_1-p1"/>
        <property type="gene ID" value="PTTG_00126"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00126"/>
    <dbReference type="OrthoDB" id="10258608at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056604">
        <property type="entry name" value="GBF1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF388">
        <property type="entry name" value="GOLGI-SPECIFIC BREFELDIN A-RESISTANCE GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23325">
        <property type="entry name" value="TPR_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="2">
        <location>
            <begin position="654"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1136"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1577"/>
            <end position="1623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1577"/>
            <end position="1598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV97389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00126-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="1623" mass="179393" checksum="9F01FB0E548A53D4" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GZD4</accession>
    <name>A0A180GZD4_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CxC1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_11885</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000012">
        <property type="protein sequence ID" value="OAV97612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GZD4"/>
    <dbReference type="EnsemblFungi" id="PTTG_11885-t43_2">
        <property type="protein sequence ID" value="PTTG_11885-t43_2-p1"/>
        <property type="gene ID" value="PTTG_11885"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_11885"/>
    <dbReference type="OrthoDB" id="3237105at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV97612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11885-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="508" mass="59005" checksum="5BAD4147DB391E83" modified="2016-09-07" version="1">MRKRNLLTSQIQNLSFGTSVFHAYVHEWACQVAYNPRFNSWWGLSDGECLERLWSFLSKLVSPLRVSTRLHRLTSIQAQADYHSEQLLEMSAQWLSNKLKNAKEVGDEAKRFLDTLHQQRNPHFPDQNQNYTDEFLEEQWILEKEYHRTTNNTLKNSRLELGGLLCLQDKLDAAWGTTALTPQQALARATTCGRLTEQIRAVRERLGTFYLTDRMTRTRGQQKLVESLRKHAEKMRSVLNLYNNHVRAYISACPDRPHPPEMEYSELLSLQSDDTFWNDGTFTNANEPWATDEATKKGIRQLSYFKRSIEERRRLGWETRRAMRWVIERHQLLKTMLQTVLRLLRNPEALPIPQDIQNLLEHAQLVTLPSILDKLKVAKLVLHAAFIKILNIQMAWHPQITDVFNKTTRQPQDGDILQLWNSHMSFIYRFHRMGLLSGVPGDICEGVISDAPMDSYPEISATGPFSLNHVHSDNEDEDKENEEDQQENELEDSLSTNLLMNLGTISNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H003</accession>
    <name>A0A180H003_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_03144</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000010">
        <property type="protein sequence ID" value="OAV97918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H003"/>
    <dbReference type="EnsemblFungi" id="PTTG_03144-t43_3">
        <property type="protein sequence ID" value="PTTG_03144-t43_3-p1"/>
        <property type="gene ID" value="PTTG_03144"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03144"/>
    <dbReference type="OrthoDB" id="2501829at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10328:SF15">
        <property type="entry name" value="BHLH TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10328">
        <property type="entry name" value="PROTEIN MAX MYC-ASSOCIATED FACTOR X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="4">
        <location>
            <begin position="491"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="566"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
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            <end position="163"/>
        </location>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="566"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="583"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="OAV97918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03144-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H0N9</accession>
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        <recommendedName>
            <fullName evidence="7">Superoxide dismutase</fullName>
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        </recommendedName>
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    <gene>
        <name evidence="10" type="ORF">PTTG_09275</name>
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    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="7"/>
    <dbReference type="EMBL" id="ADAS02000006">
        <property type="protein sequence ID" value="OAV98617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H0N9"/>
    <dbReference type="EnsemblFungi" id="PTTG_09275-t43_2">
        <property type="protein sequence ID" value="PTTG_09275-t43_2-p1"/>
        <property type="gene ID" value="PTTG_09275"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09275"/>
    <dbReference type="OrthoDB" id="239262at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.990:FF:000001">
        <property type="entry name" value="Superoxide dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.55.40.20:FF:000004">
        <property type="entry name" value="Superoxide dismutase [Fe]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Fe/Mn_Superoxide_Dismutase"/>
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        <property type="entry name" value="Mn/Fe_SOD"/>
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        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
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        <property type="entry name" value="SUPEROXIDE DISMUTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404:SF6">
        <property type="entry name" value="SUPEROXIDE DISMUTASE [MN], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
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        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase N-terminal" evidence="8">
        <location>
            <begin position="4"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="9">
        <location>
            <begin position="96"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV98617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09275-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="201" mass="22292" checksum="112475BEBD6174F8" modified="2016-09-07" version="1">MGNHTLPDLPYSYDALEPAISEEIMKLHHSKHHAAYVNGLNAAEESMANALKASDVQKMIGLQAVIKFNGGGHLNHSLFWKNLQPEKSGGGKMVEGSFSKAVQQDFGGLDQLKSAMNASAMSIQGSGWAWLGYDTKMKKLIIANTGNQDPLDPQIPILGIDMWEHAFYLQHQNRKADYLTNIWKVMNFTEAEKRFKEASGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H0Q4</accession>
    <name>A0A180H0Q4_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_11694</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000008">
        <property type="protein sequence ID" value="OAV98331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H0Q4"/>
    <dbReference type="EnsemblFungi" id="PTTG_11694-t43_2">
        <property type="protein sequence ID" value="PTTG_11694-t43_2-p1"/>
        <property type="gene ID" value="PTTG_11694"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_11694"/>
    <dbReference type="OrthoDB" id="337735at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF117">
        <property type="entry name" value="PHO85 CYCLIN PHO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV98331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11694-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="266" mass="30290" checksum="36CF047DE0652BDC" modified="2016-09-07" version="1">MTWKVGVQAEDPLGGEKHETCPDEMEALLRGPSSPPPPTRKAHPNNSEENSIDDEKEQQQQKQAQKQPTLANEDTIDNHPSTTNRLPSAGLKELPARFERCEIDDLIELIGSMLTRLINHNDQIPFTSTSLTRFHSRSPPTISINDYLRRIYKYTNPEPICLILILNYIDRICKNLINFTICSLTVHRFCITSVTIGSKFICDSFYSNSRYAKSSLSLSPLRLLICSHQTERERETIGSHSSGKIFLPFFDAAFDLLNLDNKRRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H205</accession>
    <name>A0A180H205_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial Rho GTPase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">GTPase EF-hand protein of mitochondria 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PTTG_02406</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="20 21" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="21">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the mitochondrial Rho GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000004">
        <property type="protein sequence ID" value="OAV99056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H205"/>
    <dbReference type="EnsemblFungi" id="PTTG_02406-t43_2">
        <property type="protein sequence ID" value="PTTG_02406-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02406"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02406"/>
    <dbReference type="OrthoDB" id="10020961at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000011">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000553">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013566">
        <property type="entry name" value="EF_hand_assoc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013567">
        <property type="entry name" value="EF_hand_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052266">
        <property type="entry name" value="Miro-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020860">
        <property type="entry name" value="MIRO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08355">
        <property type="entry name" value="EF_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08356">
        <property type="entry name" value="EF_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51423">
        <property type="entry name" value="MIRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="203"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="18">
        <location>
            <begin position="312"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OAV99056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02406-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="540" mass="60090" checksum="99EB7E1475952584" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H2P6</accession>
    <name>A0A180H2P6_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex protein EXO70</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PTTG_00438</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Also plays a role in the assembly of the exocyst.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Bud</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the EXO70 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000003">
        <property type="protein sequence ID" value="OAV99275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H2P6"/>
    <dbReference type="EnsemblFungi" id="PTTG_00438-t43_2">
        <property type="protein sequence ID" value="PTTG_00438-t43_2-p1"/>
        <property type="gene ID" value="PTTG_00438"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00438"/>
    <dbReference type="OrthoDB" id="1922221at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.170">
        <property type="entry name" value="Exocyst complex component Exo70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004140">
        <property type="entry name" value="Exo70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046364">
        <property type="entry name" value="Exo70_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542:SF41">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542">
        <property type="entry name" value="EXOCYST COMPLEX PROTEIN EXO70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03081">
        <property type="entry name" value="Exo70_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20669">
        <property type="entry name" value="Exo70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex subunit Exo70 C-terminal" evidence="5">
        <location>
            <begin position="222"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OAV99275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00438-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="620" mass="68577" checksum="18295203C66220C8" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H2S5</accession>
    <name>A0A180H2S5_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PTTG_06769</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000003">
        <property type="protein sequence ID" value="OAV99304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="ADAS02000003">
        <property type="protein sequence ID" value="OAV99305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H2S5"/>
    <dbReference type="EnsemblFungi" id="PTTG_06769-t43_2">
        <property type="protein sequence ID" value="PTTG_06769-t43_2-p1"/>
        <property type="gene ID" value="PTTG_06769"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_06769-t43_3">
        <property type="protein sequence ID" value="PTTG_06769-t43_3-p1"/>
        <property type="gene ID" value="PTTG_06769"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06769"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF32">
        <property type="entry name" value="ZINC FINGER PROTEIN RST2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="390"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="421"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="41"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="253"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV99305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06769-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="494" mass="54271" checksum="9D4E6E408BD6D51C" modified="2016-09-07" version="1">MIGLGSCYVDCNDLGGLPFQSSVVDHALMGFEPTFNFQEHHHSDHEVDEERSNCSPTALSPFSPHQQHASQDNQIQFHNQQQAGWQSFRSNSSALEDDQNQQPLSPTEMAALRSSSPCLAHSSSNLYFGSSPLCQYTDSGSIPPLAMELSQSPTMLRTGSTASEFSTGSSVNSTGVNPIHVNPLSQPVTPQDDIFAPLNDPFHQHWWASHNESQSVESLVSSLRASIGQPLQNSADHEAGARMPSIFAQPLKVTTSSPGEQTSSGTSSSPYSSISQPNKPPHSPSYHLGAKTRRSSHRSSLSNHNRTTSSKISSRPFLIEPDYSRLPTKRSRGRRPPISPDLGLPIPDGHLSIGLQSVPGGQAFDPNLNPHAETVKYCELTKTGKPKKIFICQVKGCGKCFKRSEHLKRHVRSIHTNDKPYNCPWMACMKSFSRHDNLNQHLRVHRVEEGGEVLNGLHIGSDGVLRSDLDESPVDPVVRGLRDVEVLDRFMVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H2T8</accession>
    <name>A0A180H2T8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="7">14_3_3 domain-containing protein</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="6">Protein BMH2, variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PTTG_05801</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000005">
        <property type="protein sequence ID" value="OAV98919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H2T8"/>
    <dbReference type="EnsemblFungi" id="PTTG_05801-t43_2">
        <property type="protein sequence ID" value="PTTG_05801-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05801"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05801"/>
    <dbReference type="OrthoDB" id="10260625at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000002">
        <property type="entry name" value="14-3-3 protein epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="5">
        <location>
            <begin position="4"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="226"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="241"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OAV98919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05801-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="256" mass="28458" checksum="9A460BD6DD17C459" modified="2016-09-07" version="1">MASREDSVYMAKLAEQAERYDEMVTYMKDVAKLNQELTIEERNLLSVAYKNVIGARRASWRIVSSIEQKEEAKGNEAQIGQIKAYRDKIEAELEVLDSHLIKSAESGESKVFYHKMKGDYHRYLAEFATGDKRTASSDASLEAYKAASSIATVELAPTHPIRLGLALNFSVFYYEILNAPDKACQLAKQAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDLNDSVPDDKEEKAAEPAAAAEGAAEPAVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H332</accession>
    <name>A0A180H332_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">pectinesterase</fullName>
            <ecNumber evidence="3">3.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Pectin methylesterase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_00017</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pectinesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="3"/>
    <dbReference type="EMBL" id="ADAS02000004">
        <property type="protein sequence ID" value="OAV99204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H332"/>
    <dbReference type="EnsemblFungi" id="PTTG_00017-t43_2">
        <property type="protein sequence ID" value="PTTG_00017-t43_2-p1"/>
        <property type="gene ID" value="PTTG_00017"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00017"/>
    <dbReference type="OrthoDB" id="2506391at2759"/>
    <dbReference type="UniPathway" id="UPA00545">
        <property type="reaction ID" value="UER00823"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321">
        <property type="entry name" value="ACYL-COA THIOESTER HYDROLASE YBHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321:SF57">
        <property type="entry name" value="PECTINESTERASE 53-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pectinesterase catalytic" evidence="7">
        <location>
            <begin position="64"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OAV99204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00017-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="201" mass="21968" checksum="E2252029B6298917" modified="2016-09-07" version="1">MTAIMLANKLFTVPQWYFLILFSFAIFAASHALPTHSLSPRANPSSTVPPTGALIVRQKNPLPGEHPTIPDAIRALRGTKGPQTIFVYPGSYNLGVYIRYGYPLTIQGYTAAPGTYAQNQVTVSVATGRAETGGDPQSSAIWVQQNDFKMSNINVVNSYGTGTNTQAVAVTAQGERHVYSMCSFSSYQDTLFHLWGWDRMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H363</accession>
    <name>A0A180H363_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_02397</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000004">
        <property type="protein sequence ID" value="OAV99068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H363"/>
    <dbReference type="EnsemblFungi" id="PTTG_02397-t43_2">
        <property type="protein sequence ID" value="PTTG_02397-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02397"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02397"/>
    <dbReference type="OrthoDB" id="511599at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36168">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36168:SF1">
        <property type="entry name" value="ORC1-LIKE AAA ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV99068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02397-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="390" mass="42918" checksum="CC5CA88678BE21CC" modified="2016-09-07" version="1" fragment="single">MTPASFRLSAQLVTPLSRALSARPARPAWHPNARGPRLASYSSAGSSSSSQQQQERPSSSWTDGVAAQAVFGSLGGVLILGFGGSVLLLLQLRLLFVYPVRTGLSACPSAFQVSLPHLKMAVAFEPGYDPVLVLNKASERVPRKPELSRPSYKPGVSTEEDDGCPEGEDEEQVEEVVWIARREQELIDQIIHGKLAGQYWLITGPKGTGKFSLIADAMTRNQADGQSSIQHKPLPGLSSLLTAPNTSPELTQNHAFFSSVHLRQDEGSYSLLLQLQQRAESWSQAGIVTIVFCSDDHWPYASLKKNASRMSVLTVKDLTKQEAVRAIRTERMKLWREARPADDPEIEALWTLVGGRIAHLSGCMKHKDMLHAAKLIIEREKQWLLSRIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H365</accession>
    <name>A0A180H365_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Replication factor C subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_05338</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000005">
        <property type="protein sequence ID" value="OAV98803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H365"/>
    <dbReference type="EnsemblFungi" id="PTTG_05338-t43_1">
        <property type="protein sequence ID" value="PTTG_05338-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05338"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05338"/>
    <dbReference type="OrthoDB" id="4199794at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000012">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="ATPase_AAA_core"/>
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    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF5">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="34"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OAV98803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05338-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="323" mass="35721" checksum="E37BEA4BFCFAB440" modified="2016-09-07" version="1">MFFRVEKYRPIILDDITGNTDTIERLKVIARDGNCPHIIISGAPGIGKTTSILCLAHALLGDAYKEGVLELNASDERGIDVVRNKIKTFANTKVTLPAGRHKIIILDEADSMTAGAQQALRRTMEIYSNTTRFALACNNSSKIIEPIQSRCAILRYGKLKDQEVLKRLVEIASAENVKYAEDGLAALIFTSEGDMRQAINNLQSTVSGFGFVSAESVFKVCDQPHPVVIQKLLSSCEKGDLSDALDQLEVLWEQGYSALDIISTLLKVVKGMDRMSEFLKLEFIKEIGFTHMRILEGVSTLVQLGGLLSRLCKLKIDPKKFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H372</accession>
    <name>A0A180H372_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_25382</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000003">
        <property type="protein sequence ID" value="OAV99445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H372"/>
    <dbReference type="EnsemblFungi" id="PTTG_25382-t43_1">
        <property type="protein sequence ID" value="PTTG_25382-t43_1-p1"/>
        <property type="gene ID" value="PTTG_25382"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_25382"/>
    <dbReference type="OrthoDB" id="107110at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV99445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_25382-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="342" mass="38163" checksum="AEB1100864D9464F" modified="2016-09-07" version="1">MARHVSTFYPSQPIYALTANDYLYQNEDELVLCIDSLARVLSRGCVDTGEAGEYASRIILLCAMNKTVHDLKTLDKARDGTDNKAPDGMDIDSPPDDMKTLDKAPKTSNKAPNIPDSRPVELKEFPGAIPVARFLETLTGISANTLPLGSIASEQKKRLLEEGMMHWNHFIQCTKTPTTESLMEGLHRGVAMQCQHSQKAFDQVLTIYLKERSRDFLEPKNISFCGVQVKNVPHDTAAQIYQSLMTPENAGIDIPNVNPYLALFFDLKYSPQIDKQAQAGDPENRVKTYQLPAQGEPDVRQASLVFYGLDAFNFLSPKLKQALRKLINIKVDLLLRHADVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H3S2</accession>
    <name>A0A180H3S2_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_02428</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
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                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
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                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
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                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
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                <person name="Nusbaum C."/>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
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    <reference evidence="2" key="2">
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
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                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
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    <reference evidence="3" key="4">
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
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            <dbReference type="EMBL" id="OAV99232.1"/>
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    </evidence>
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                <person name="Earl A."/>
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                <person name="Zeng Q."/>
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                <person name="Borenstein D."/>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
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                <consortium name="EnsemblFungi"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <dbReference type="EMBL" id="ADAS02000003">
        <property type="protein sequence ID" value="OAV99302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H461"/>
    <dbReference type="EnsemblFungi" id="PTTG_25343-t43_1">
        <property type="protein sequence ID" value="PTTG_25343-t43_1-p1"/>
        <property type="gene ID" value="PTTG_25343"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_25343"/>
    <dbReference type="OrthoDB" id="2138242at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="1"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="455"/>
            <end position="533"/>
        </location>
    </feature>
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            <begin position="559"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="737"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="676"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
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            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV99302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_25343-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="804" mass="85612" checksum="6DDF5416E18F9AFD" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H467</accession>
    <name>A0A180H467_PUCT1</name>
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        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PTTG_04546</name>
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    <organism evidence="12">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="ADAS02000003">
        <property type="protein sequence ID" value="OAV99389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H467"/>
    <dbReference type="EnsemblFungi" id="PTTG_04546-t43_1">
        <property type="protein sequence ID" value="PTTG_04546-t43_1-p1"/>
        <property type="gene ID" value="PTTG_04546"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04546"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="11">
        <location>
            <begin position="164"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OAV99389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04546-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="368" mass="42217" checksum="CC6437D5ED616D8E" modified="2016-09-07" version="1">MGVIRTVAHKVFRCFWRIERCADRLTGKVGPFFIAIAVLLITMAIVTYFDVIFPMAFLQPECDPLMSVLSLIFSVYLSACSMIHYYFACTTAPGSPSEPINQPNKSFSLGRSSCARGLRRWLPRGQRIGPVKQEMIEQYRANINRHCLKCLKNGIKGVDGRGPIKPERAHHCRVCGVCQLKYDHHCPWINQCVGLRNERFFLLFLFYMTVSCAWVVFWGWPSFTESLDFSTSWPFWSPRIFVILTWVLALAIGVTIGIMFGWQLLLIAKGETTVESSDNEYYHQLFSQRGQKYVNPFDLGVRGNLEQFFNTGRGGRCGWYTVLLPLKFPPSNDGWSWPKRAGWENQVINLAEELTDEEDDEDDHPTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H4Y7</accession>
    <name>A0A180H4Y7_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">HTH APSES-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_07047</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="OAV99854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0A180H4Y7"/>
    <dbReference type="EnsemblFungi" id="PTTG_07047-t43_1">
        <property type="protein sequence ID" value="PTTG_07047-t43_1-p1"/>
        <property type="gene ID" value="PTTG_07047"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_07047"/>
    <dbReference type="OrthoDB" id="6718656at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="APSES transcription factor (MbpA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051642">
        <property type="entry name" value="SWI6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828">
        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF3">
        <property type="entry name" value="CHROMO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04383">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="29"/>
            <end position="135"/>
        </location>
    </feature>
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        <location>
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            <end position="363"/>
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    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="452"/>
            <end position="484"/>
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    <feature type="domain" description="Peptidase A2" evidence="6">
        <location>
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            <end position="481"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="194"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50175"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51299"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="OAV99854.1"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblFungi" id="PTTG_07047-t43_1-p1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="815" mass="89964" checksum="170E8FA650237EA0" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H581</accession>
    <name>A0A180H581_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Type 1 phosphatases regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_05953</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Regulator of type 1 phosphatases which maintains protein phosphatase activity under strict control.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the YPI1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="OAW00116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H581"/>
    <dbReference type="EnsemblFungi" id="PTTG_05953-t43_2">
        <property type="protein sequence ID" value="PTTG_05953-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05953"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05953"/>
    <dbReference type="OrthoDB" id="307488at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011107">
        <property type="entry name" value="PPI_Ypi1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07491">
        <property type="entry name" value="PPI_Ypi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="74"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAW00116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05953-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="165" mass="17664" checksum="6A99C4180D016D5A" modified="2016-09-07" version="1">MVIDQDSSSSSGPQNENHANNPNTAPTLILRAGPLQDSQLPRQPRVQWQENVVDNEGLGRKKSKVCCIYNKPKAFDESSDESDHSDSDCCPSRSPAPRPRPSTNSTTQARLGSPSQPAQSHSLPTPPRSPNAYEAEPSKSKGKGKACLNVGFAETAINDSPSNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4EH34</accession>
    <name>A0A0C4EH34_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PTTG_00050</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
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                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
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                <person name="Yin C."/>
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                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV90236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00724-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="368" mass="40989" checksum="8A75217AAE831A1F" modified="2015-04-01" version="1">MPPWLAPNTITLIGLMFIVLNVMTVIIYIPDLKTDAPSWVYFSFALGLFIYQTMDNVDGKQARKTGTSSPLGELFDHGIDSLNCVLGGLVQCAAVGTGHSLYAVFILIVACWPMYLSTWEEYHTGILYLGFVNGPTEGLLIAMAVLLSTGFGGVQMWSKPAEDLVYLPRLILTYCDKFGHQLKLLDLFVSFVLFALVFGHAPSCFYNTYVAIRNNQSQPRSKQDSRISSFSQALLRLFPLLIFTFCSASWLASSNSHILKSEKIIEFGLMLCLIFGRIATRIILSHLTKSTFPFWSGMLIPIIGGSIAINLPMIGLPAFLSPWGELVYLRLMFFFTIVGYFGSSLRTIDRFCQVLRINCLTIRSKSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4ERG7</accession>
    <name>A0A0C4ERG7_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_03385</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000040">
        <property type="protein sequence ID" value="OAV94346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4ERG7"/>
    <dbReference type="EnsemblFungi" id="PTTG_03385-t43_2">
        <property type="protein sequence ID" value="PTTG_03385-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03385"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03385"/>
    <dbReference type="OMA" id="ISQHANL"/>
    <dbReference type="OrthoDB" id="2565191at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.80">
        <property type="entry name" value="E3 ubiquitin-protein ligase SopA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141571">
        <property type="entry name" value="Pentapeptide repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV94346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03385-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="308" mass="34645" checksum="08DB4E55B6E80133" modified="2015-04-01" version="1">MVPSIQPGRRKIDPSCRPQYQKLIDGDSIYTCSKFTSEREPRLGDSIFRPSKKFKKSLMVEHGPWFLDLEPVLERRDQGRHRLWKRQKNKRLKRISSAARPNPQQAGPQQANSQHADPQQTDPQLQTAYSQRTDRLTAEPQHTDLQPTDLQPTDLQPTDLQHTDLQQTDLRAAYLPQADPQHAISQHANLQQADLHQADDEDADRTITLAAFGTQGTCSLPIDNTVEFPPRAVTPDLPSSDLLRSIHHEAATFYSSHNVLSIPTPHRGPRPKNWEEPIAMARALDGSALVAIGVLLEEIAKDQVESAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4ESF4</accession>
    <name>A0A0C4ESF4_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial import inner membrane translocase subunit Tim21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PTTG_03729</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TIM21 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000075">
        <property type="protein sequence ID" value="OAV91797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4ESF4"/>
    <dbReference type="EnsemblFungi" id="PTTG_03729-t43_2">
        <property type="protein sequence ID" value="PTTG_03729-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03729"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03729"/>
    <dbReference type="OMA" id="YRIHIRT"/>
    <dbReference type="OrthoDB" id="436405at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013261">
        <property type="entry name" value="Tim21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13032">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13032:SF6">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08294">
        <property type="entry name" value="TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV91797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03729-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="375" mass="42573" checksum="344CC5DDDC009102" modified="2015-04-01" version="1">MRSISNGFLHQLQPSRTCVCPILSKLYPAIPAANLLNPLRQYSRPSSSPPLNRRLSTNWTRNAVSARSKKDSRIINSRSYVSASGHPPKQSEEALMEELRRLSHGQESGQPMLGPFMMPRGKAHEKPLRDDEYHHWSRVSWSERIRRMFRMGRNLLIVSFGGSLGILVVRFNKIYSITSELFAPSSTTNLMNMTIRTIEESDQLSKILKSPIKFHTSPTPSGNSISKRSNGIPQSVQHQSGVVELRFWVESCKPSLFEGDWKSLDWWRSWIGPLITSEIYHHPTNRSSDSISNSIDSQLSGSDSNSTRWWPGLLKSLMPNTLGRSSSSANEQKSWTARFFSNHGSFEHGEVVAQLIKVRSIRFKIYVEDSCLSDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4EVK8</accession>
    <name>A0A0C4EVK8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="10">TPR_REGION domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_04844</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NASP family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000026">
        <property type="protein sequence ID" value="OAV95729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4EVK8"/>
    <dbReference type="EnsemblFungi" id="PTTG_04844-t43_2">
        <property type="protein sequence ID" value="PTTG_04844-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04844"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04844"/>
    <dbReference type="OMA" id="QTYILMA"/>
    <dbReference type="OrthoDB" id="2505766at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051730">
        <property type="entry name" value="NASP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019544">
        <property type="entry name" value="Tetratricopeptide_SHNi-TPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15081:SF1">
        <property type="entry name" value="NUCLEAR AUTOANTIGENIC SPERM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15081">
        <property type="entry name" value="NUCLEAR AUTOANTIGENIC SPERM PROTEIN NASP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10516">
        <property type="entry name" value="SHNi-TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="264"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide SHNi-TPR" evidence="8">
        <location>
            <begin position="265"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="141"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="349"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="445"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="357"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="452"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OAV95729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04844-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="489" mass="51841" checksum="1B90389B8F452DEC" modified="2015-04-01" version="1">MFRSKPRVRSRPPDAGLGHTLDGPFQNSTKQSQTMSDSLSNQPSEDPKGKQEQVAAAQSEEANDTDIDTIEAELAAGARAFAFKNYAIATECFSKACEKLTTHHPENHTAIIDAHLSYGKALLRNAITLGSLSGGLNAHNPAAAATEPGDAAPEVTESAASSSKGTANQAGAASSSKPSQPVAKGNKGNPQIHFSGDSSDEDDDEGAGEDGDEEIDQAEEPVTVEDELENAFQVLEFARQSILSQVDSSKDQPEQAEKLQNKLIDVYDLIAQVHQEGEQFEQAVESYKSSLEVKKSRTDVTAGAIAETHLMIALALELIPDEGESMKQAIGHVDEAIELMKAQLAKLTEQAGGQTEEGGKEKPTESSEGTKQDVGPANKLKSEIEETQSLIGELEAKREELKTVPQAPAMTEADKALQAYLAAVNNNPSTANGKAAINDLTSLVKKRARPVDSSSDPQPNTSSSSSALPSTTDTNLPPETPSKKIKTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4EWP2</accession>
    <name>A0A0C4EWP2_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_05237</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02001170">
        <property type="protein sequence ID" value="OAV86415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4EWP2"/>
    <dbReference type="EnsemblFungi" id="PTTG_05237-t43_1">
        <property type="protein sequence ID" value="PTTG_05237-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05237"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05237"/>
    <dbReference type="OMA" id="RWGAMWE"/>
    <dbReference type="OrthoDB" id="10050400at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040201">
        <property type="entry name" value="Mrg3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28142">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE I-AAA PROTEASE SUPERCOMPLEX SUBUNIT MGR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28142:SF1">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE I-AAA PROTEASE SUPERCOMPLEX SUBUNIT MGR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV86415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05237-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="387" mass="43293" checksum="42D87ABDA98E4F89" modified="2015-04-01" version="1">MNQLFRRTLLVGLTRRKPPRLNHAYSTGPNSKRTAAGAGLILGIPLTAYLIYRANTQPAYPDEIQRLLRQALIAEHRSDHRKSDILFRQAYEQSKQLVRSGELDWLKSSAVAIRWAASLEQTARTDRAIEVYQLVFDDLRLALPGLSVHERLRTIEIAQKLGQLLIHHSPTPPGKPAESVEIKIEECLVFSVEEILKIRNENNNFALDSDERQQMLPVSDQYQELFESLPSWVARLQFASCFEALGKFYAQRGQTDFALPLYLQTLNLLLPPQQDSGKKWNKQGPTDEERCQAATVMNNIGQLLFAHSVTDAGAADGGSDRLRLNAADWLGQSAALARRVLDAHQQLPALDQPRPLDTPPNGFLAECQEAFVAASANLKSLNHPPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4EX21</accession>
    <name>A0A0C4EX21_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PTTG_05368</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000014">
        <property type="protein sequence ID" value="OAV97220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4EX21"/>
    <dbReference type="EnsemblFungi" id="PTTG_05368-t43_1">
        <property type="protein sequence ID" value="PTTG_05368-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05368"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05368"/>
    <dbReference type="OMA" id="RYDYPNG"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000007">
        <property type="entry name" value="Oxysterol binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2720:FF:000001">
        <property type="entry name" value="Oxysterol-binding OBPalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF102">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="395"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OAV97220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05368-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="453" mass="51595" checksum="C7A75E962E64B1D9" modified="2015-04-01" version="1">MMGVFSAVAARAGIKDPTTQPNDRTQEENEEASDEEVLDEEPKHMLLALISQLRVGMDLHKVTLPTFVLEPRSMLERITDFLSHPDLIFGVSQQEDPTERFIQVLRYYLAGWHIKPKGVKKPYNPILGEFFRCRYEYPDGSQGFYIAEQVSHHPPISAFYYVSPTNQIEIWGDLRPKSKFLGNSAATIMEGVSHLRFVDREEDGHYQITMPNMYARGILFGKMILELGDESRVQTAANDIYCDVEFKTKGFFGGDYNAIGGRVRDQTGIVGEISGKWNEVMELKRTKGKTETIFDASKATVTQKIVQPEQDQDWNESRRLWSKVTRAIKKKDLDAATLAKTAIEDFQRDRARLREEKEEKWEPVFFELRDEDEWKPKFTLPEDKQERIKAVEKFIFDYHSTPPPDNPTDQASSAPPPTSPTQTHCPKPDRRSSTTASSPPPAAAKNLDQSASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4EYS0</accession>
    <name>A0A0C4EYS0_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AMP-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_05975</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000019">
        <property type="protein sequence ID" value="OAV96456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4EYS0"/>
    <dbReference type="EnsemblFungi" id="PTTG_05975-t43_2">
        <property type="protein sequence ID" value="PTTG_05975-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05975"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05975"/>
    <dbReference type="OMA" id="FEDSHIY"/>
    <dbReference type="OrthoDB" id="6509636at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF295">
        <property type="entry name" value="ACETYL-COA SYNTHETASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="1">
        <location>
            <begin position="147"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV96456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05975-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="456" mass="50621" checksum="A48607AE50F33F63" modified="2015-04-01" version="1">MLLGALAAGLTFTMVNPSYTRHELVHVLRSAQPALIVTSSFGLDVMLDALTLLREPHLRRQLMERIFIVDAPGSAPSRFFEDSHIYRTKAPNGEIATLTDWNALLQQPPSGTHYRPFQFSEPARESRLRIAAVTWSSGTSGKRLQTIVLFSIVTGSTVYIMQKFEPHRYLEEITKNRITSLQIAPPIAAFLAKSPLLDDARYDLSSVTNSMSGGAPLAPAIVKTVFKRCGFLIKFGYGLSEAGHVANQLGATMSDLLPQLGTVGEVMYGLELKIISVRTKQLVLRNEEGEIIVRSPSLMSAYLDNPSETSQVLDEDGWLHTGDLGFLDDHGRLSITGRLKEIIMVKGYQVSPSDLEMLISKIDAVGEVAVTSFYCDDQATEFPRAYIVPQGDELVALCEIVNSNSSPCEPVHHPRYNDLTKLALQVKEFVESHMVQYKWSVCTVCDLRYHSIFGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4EYS7</accession>
    <name>A0A0C4EYS7_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_05982</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000148">
        <property type="protein sequence ID" value="OAV88904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4EYS7"/>
    <dbReference type="EnsemblFungi" id="PTTG_05982-t43_2">
        <property type="protein sequence ID" value="PTTG_05982-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05982"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05982"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV88904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05982-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="68" mass="7434" checksum="0F17138C1F7B5151" modified="2015-04-01" version="1">MADYPEQYIISSDDSASTVSLDAPHVADTPSPVSDDDDPYSYHNIFMASSDEASDEDIRSDDDDQGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0C4F5D3</accession>
    <name>A0A0C4F5D3_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA replication complex GINS protein PSF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_08332</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GINS1/PSF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000044">
        <property type="protein sequence ID" value="OAV93984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0C4F5D3"/>
    <dbReference type="EnsemblFungi" id="PTTG_08332-t43_1">
        <property type="protein sequence ID" value="PTTG_08332-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08332"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08332"/>
    <dbReference type="OMA" id="CLMAYHN"/>
    <dbReference type="OrthoDB" id="10252587at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11710">
        <property type="entry name" value="GINS_A_psf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21696">
        <property type="entry name" value="GINS_B_Psf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1030:FF:000018">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005339">
        <property type="entry name" value="GINS_Psf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056783">
        <property type="entry name" value="PSF1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914:SF2">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914">
        <property type="entry name" value="PARTNER OF SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24997">
        <property type="entry name" value="PSF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication complex GINS protein PSF1 C-terminal" evidence="2">
        <location>
            <begin position="192"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU368085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV93984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08332-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="243" mass="26726" checksum="D7DA52FBE2EFAB4E" modified="2015-04-01" version="1">MSYTNAAGTTGVPFGAQNALLTASVRERTTDTIKPFEETLVRTLQRETRAFGDQLTQAAEHFRKQQEALDEEDIEMDPGTICALKMSASTARHNKRILLAYAMQRRNAILDCYWACSASVPNTLVAPSTNVSADKSEGGSERAGEPISKVLTPVELEYLRAHSTLVHDLAKPYQAYTVNLLGNLARGPARGLFIQVECMRELGEVAVGDGGGMIKFEVGSRYFVKRSEVDQLIEGGWLMEIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G053</accession>
    <name>A0A180G053_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_12762</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02001805">
        <property type="protein sequence ID" value="OAV86060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="ADAS02001805">
        <property type="protein sequence ID" value="OAV86061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G053"/>
    <dbReference type="EnsemblFungi" id="PTTG_12762-t43_2">
        <property type="protein sequence ID" value="PTTG_12762-t43_2-p1"/>
        <property type="gene ID" value="PTTG_12762"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_12762-t43_3">
        <property type="protein sequence ID" value="PTTG_12762-t43_3-p1"/>
        <property type="gene ID" value="PTTG_12762"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12762"/>
    <dbReference type="OrthoDB" id="2498397at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="10"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV86061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12762-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="396" mass="44848" checksum="9D5E81BDD1E688FF" modified="2016-09-07" version="1" fragment="single">PRTSTMSACSRLPSELLVEIFNWLPPLSHSSDRLSVSSVCRSWLGPAQIVLWRSLWLRSPSSVSSLAETVRQKPGLAKLPRALHFSTGDDEYLAELDADAVFTTMERLVNLEQFHVLVNYCMGDLYDSLCEELLGSPIKHIVLGADEGISRSHVQASMRFERLEYLELLRLGDLGDLKSLELEWVFSESLAELVLIQPDVPGDALRFLLSHVKGTLKRLRIHFGHLLADNGEDGSTTRITEEDLETALIESGTDLRHLELSWPYSTRPFLNEAVKGLFSLEDMYISGGICDPGLIDLNCQSKLERISFDLRPGTEPPKSLLRLVEPSARRSNLTKYHARTFFDDPSWEQSVLKMVEMRDQDQEIPIKDWSKECIEKWQDLSKKLEASGIKLTGDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G083</accession>
    <name>A0A180G083_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_30142</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02001958">
        <property type="protein sequence ID" value="OAV86004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G083"/>
    <dbReference type="EnsemblFungi" id="PTTG_30142-t43_2">
        <property type="protein sequence ID" value="PTTG_30142-t43_2-p1"/>
        <property type="gene ID" value="PTTG_30142"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_30142"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV86004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_30142-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="198" mass="22238" checksum="21807A5A00F0EE58" modified="2016-09-07" version="1">MPHMYSFHWLLHCMLDDKANGQVDRNRIGGQPYLVTSNQNNRHPLIGPVNPQTTGSFANMNYNNFENRPLRDGLNLNNGQARTILTPNPNQHAHQQAHHLDHRQQQPSTRFVFQSNNHLLAATGPYAHLQPQANNIGHPVPLPTNRLPPAMLLDIELINQKHSGFVLANNQQRQPPFHDPILGGQTSLCHTGLLGRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G1Z5</accession>
    <name>A0A180G1Z5_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">tRNA(m1A58)-methyltransferase subunit TRM6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_04725</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRM6/GCD10 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000913">
        <property type="protein sequence ID" value="OAV86667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G1Z5"/>
    <dbReference type="EnsemblFungi" id="PTTG_04725-t43_2">
        <property type="protein sequence ID" value="PTTG_04725-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04725"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04725"/>
    <dbReference type="OrthoDB" id="10254665at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017423">
        <property type="entry name" value="TRM6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945:SF0">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT TRM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04189">
        <property type="entry name" value="Gcd10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="188"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OAV86667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04725-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="362" mass="40780" checksum="0E646EFCD9936553" modified="2016-09-07" version="1">MIKESDGAQKLTNQEIEELKKSGLSGREIILRQIQQHSAFELKSEFSKAKYIKRKEKKFLKMFTCIDPTIHNLSQYLFENHHFAVKGLRPDTLSQMLSLSNIRPGWRGIVVDDIGGLLVAAALIRMGGQGSIFVINNADSPPDLHLLELFNLPTSALAPLKSLNWAQIEADWTTNEIEELLSLHQTPPQPASLLNHTPSDPQVDNPADRSKKEPNNQSKSRKKFQRVQALLNVRQEFFESQFEGLITCSEYEPESIVSNLLDRLMASSTIVIYSCHLRPLSDLQTLLKKSSVPSTSSSSSLVEQNELTRKMKESKTEFIQITISEPWLRVYQVLVGRTHPEMNGTHHGGFIFSAIKVISSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G2Z7</accession>
    <name>A0A180G2Z7_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Shugoshin_C domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_07613</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the shugoshin family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000605">
        <property type="protein sequence ID" value="OAV87075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="ADAS02000605">
        <property type="protein sequence ID" value="OAV87076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G2Z7"/>
    <dbReference type="EnsemblFungi" id="PTTG_07613-t43_2">
        <property type="protein sequence ID" value="PTTG_07613-t43_2-p1"/>
        <property type="gene ID" value="PTTG_07613"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_07613-t43_3">
        <property type="protein sequence ID" value="PTTG_07613-t43_3-p1"/>
        <property type="gene ID" value="PTTG_07613"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_07613"/>
    <dbReference type="OrthoDB" id="2507001at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045132">
        <property type="term" value="P:meiotic chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011515">
        <property type="entry name" value="Shugoshin_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07557">
        <property type="entry name" value="Shugoshin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shugoshin C-terminal" evidence="4">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="274"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
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        <location>
            <begin position="121"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
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            <begin position="303"/>
            <end position="375"/>
        </location>
    </feature>
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        <location>
            <begin position="376"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OAV87075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_07613-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="418" mass="45072" checksum="53478779BE022122" modified="2016-09-07" version="1">MQLPTTNSADTMSRVSGASSPGPSNQPAKLLLDRFVVSRPNRRHELMAPVPKKSSNSRAGPEPIPNGHSKTSAQHAQLEVTEEAYDDRSEKQDSHDDAENQSLEDDHTCSGSFSIHNRAMSQLDQSSSSSNSSESDHDSQDDEDSAELNENIHVNARPKDQKALCRTHYTIAAAGCARQPEALRDQPTSVKRRDSGLTKEDTDDGPDESQPSQDEGWEKRPVTKKKRVRAELDAVSEEHAASAHPTGLVEQEAAVGSLSGAGLREARRARKEVNYALPSLNKKMRRPDSDGASTVKRKSSIKPPSTHHSSHLNTSTSSSSSTKPPKSSASVKSSGATPSTAGPSNTSYSTTTSLNLDSSPLTSPSSSTNSRRSNTCAPSTLQLKNPPVNSQHKAPLQPSSLDNKDILLNRGRRRTHNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G4C8</accession>
    <name>A0A180G4C8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_11608</name>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000382">
        <property type="protein sequence ID" value="OAV87491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G4C8"/>
    <dbReference type="EnsemblFungi" id="PTTG_11608-t43_1">
        <property type="protein sequence ID" value="PTTG_11608-t43_1-p1"/>
        <property type="gene ID" value="PTTG_11608"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_11608"/>
    <dbReference type="OrthoDB" id="2507591at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="681"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="792"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="855"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="326"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="617"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="855"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV87491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11608-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="974" mass="102112" checksum="CC02A486E46E1466" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G5B2</accession>
    <name>A0A180G5B2_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GST N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_29277</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000281">
        <property type="protein sequence ID" value="OAV87790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G5B2"/>
    <dbReference type="EnsemblFungi" id="PTTG_29277-t43_2">
        <property type="protein sequence ID" value="PTTG_29277-t43_2-p1"/>
        <property type="gene ID" value="PTTG_29277"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_29277"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF8">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE GSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="108"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV87790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_29277-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="23863" checksum="900B659CA3A3F0A0" modified="2016-09-07" version="1">MANTRTPKPLRLITMNTPKGQEVQIVVEELRLCYEVNVVHMVVDVRTGMQKQPGFLRLNPNGKIPVLIDNNHDAINTIDRPFCVMESGAICRYLCSRFDTKRVFYFSDPCELSEMEQWVAWGLSTLNPATDECIKSLRHSDQRDDSAVKYLRDEVILAYTDLENHLQSASPLGTRLYLAGNGQGKFSIADMIIWPWINVSHMCGLREMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G5C3</accession>
    <name>A0A180G5C3_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_29334</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000320">
        <property type="protein sequence ID" value="OAV87659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G5C3"/>
    <dbReference type="EnsemblFungi" id="PTTG_29334-t43_1">
        <property type="protein sequence ID" value="PTTG_29334-t43_1-p1"/>
        <property type="gene ID" value="PTTG_29334"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_29334"/>
    <dbReference type="OrthoDB" id="2497884at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV87659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_29334-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="80" mass="9162" checksum="E0B344C21A6E03FA" modified="2016-09-07" version="1">MEPLAALHDLISQLIVPHRRHSSTNLINRLVADIWAIRMELRWACLLDGIAISEEMSIQIADGMKNRNPNKSIDKQTCKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G6B6</accession>
    <name>A0A180G6B6_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_09094</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Zeng Q."/>
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                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
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                <person name="Zeng Q."/>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000161">
        <property type="protein sequence ID" value="OAV88655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G7H8"/>
    <dbReference type="EnsemblFungi" id="PTTG_08938-t43_2">
        <property type="protein sequence ID" value="PTTG_08938-t43_2-p1"/>
        <property type="gene ID" value="PTTG_08938"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08938"/>
    <dbReference type="OrthoDB" id="6745403at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008568">
        <property type="entry name" value="EMC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116:SF5">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010045">
        <property type="entry name" value="DUF850_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="70"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR010045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV88655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08938-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="261" mass="29410" checksum="48E3FD363EEBBF49" modified="2016-09-07" version="1">MLLVGILRHYAMQLLHTDPKPLGLRQLREQRALMRSACFRNNCNFLSPARFEERRNRLIDAFAEGHYLSDSSGTNSKTGTDGTLKTAGNNNELPANPPNPLDPANMEGMMDGMKKQMVMMIPQTVIMGWINAFFFGFVCVKLPFPLPNGFKQMLQRGIETRDMDISWVSSLSWYFLNLFGLNSLYRLILGDGNAADGTASLAMGGASNMGAVMPGQQMDYKKLFQTEIENLKLIDHQYVCDPIPNLQLDDIESRVLQMYQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G8I5</accession>
    <name>A0A180G8I5_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SBDS family rRNA metabolism protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_09155</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Associates with the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SDO1/SBDS family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000197">
        <property type="protein sequence ID" value="OAV88213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G8I5"/>
    <dbReference type="EnsemblFungi" id="PTTG_09155-t43_2">
        <property type="protein sequence ID" value="PTTG_09155-t43_2-p1"/>
        <property type="gene ID" value="PTTG_09155"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09155"/>
    <dbReference type="OrthoDB" id="10253092at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.900">
        <property type="entry name" value="SBDS protein C-terminal domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046928">
        <property type="entry name" value="SDO1/SBDS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018978">
        <property type="entry name" value="SDO1/SBDS_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037188">
        <property type="entry name" value="Sdo1/SBDS_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927:SF1">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20268">
        <property type="entry name" value="SBDS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09377">
        <property type="entry name" value="SBDS_domain_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109728">
        <property type="entry name" value="Hypothetical protein AF0491, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS N-terminal" evidence="6">
        <location>
            <begin position="2"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS central" evidence="7">
        <location>
            <begin position="81"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS C-terminal" evidence="8">
        <location>
            <begin position="147"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OAV88213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09155-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="26300" checksum="D22F1F4954DA9E2C" modified="2016-09-07" version="1">MEWRSGVETDLSEVIQTDTIFSNVSKGQLASHSDLLKSFPVPNKNPATVLPSEDILLEILKNGELQVSDKERAQALVDKKREIIHLVAEKTVDPLTNRPHTSSMIEKALEELHFSVNLSKSSKTQALELIKSLSSRPEIISISRSQMKIRLTVPTTKESKKLKPIILESTTKVELDETDEDQWELIALIEPGQFKFISDTLNQFHLKNNSTSAPRFKLETLSLTAEQVEGDEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G8I9</accession>
    <name>A0A180G8I9_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Pheromone-processing carboxypeptidase KEX1</fullName>
            <ecNumber evidence="14">3.4.16.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Carboxypeptidase D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Pheromone-processing carboxypeptidase kex1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PTTG_08269</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="21 22" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="22">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="21" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential release of a C-terminal arginine or lysine residue.</text>
            <dbReference type="EC" id="3.4.16.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.6" evidence="14"/>
    <dbReference type="EMBL" id="ADAS02000143">
        <property type="protein sequence ID" value="OAV89026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G8I9"/>
    <dbReference type="EnsemblFungi" id="PTTG_08269-t43_1">
        <property type="protein sequence ID" value="PTTG_08269-t43_1-p1"/>
        <property type="gene ID" value="PTTG_08269"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08269"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF190">
        <property type="entry name" value="PHEROMONE-PROCESSING CARBOXYPEPTIDASE KEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="416"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="510"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="455"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OAV89026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08269-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="531" mass="59569" checksum="FAEC55D06FD6185D" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G8J0</accession>
    <name>A0A180G8J0_PUCT1</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_07064</name>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000162">
        <property type="protein sequence ID" value="OAV88642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G8J0"/>
    <dbReference type="EnsemblFungi" id="PTTG_07064-t43_2">
        <property type="protein sequence ID" value="PTTG_07064-t43_2-p1"/>
        <property type="gene ID" value="PTTG_07064"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_07064"/>
    <dbReference type="OrthoDB" id="2495815at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV88642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_07064-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="501" mass="58000" checksum="B938104CB680D8B8" modified="2016-09-07" version="1">MAERVPDEQPAPAHPQVDLDSLDMNEPIPVGFTPILDQVQKAIVNPQLRIKYDAPMKKQIASYRVKYPLQRANRLNEQVGDLPIYFEKCSAGTSSQQMLSPRIAKRAKPKGPPAKYEGLYQHPSMIAHWLGSLIKCVLFAHDAFLQQIHTPVEEQTKKHDSLIRWLNELVFSTTNHLPIGRGDDRNFKFYNDFITLTLIGIWYKREAPNEWAQHFQGSESTFWLRMRESMEEAKKDRRCRASKWVEQGRGDQRPDAEVVDFELRALQQGINPQGDAHSWLAEQSWAVHRNKDEPSEVEKRIISEYRRVLELIELKRTGKPNKTITDIPAKLHDAVERSTSKEIWMVRVIDSMSRAQTTKSVESRLTALLEGLRQYHQNLISSMEHSGIQTIPNAHNAFLDWFGGTILKPSPGPEHLSLFGYIYKSQIGDAELPLDETRFTPIQNLVLRFIAMPDRTREIPLTLLGYWYKKYQPAFWQKHFQDELRFGMLFKHTKHNTSDVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G8J2</accession>
    <name>A0A180G8J2_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Pheromone-processing carboxypeptidase KEX1</fullName>
            <ecNumber evidence="14">3.4.16.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Carboxypeptidase D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Pheromone-processing carboxypeptidase kex1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PTTG_08269</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="21 22" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="21">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="21" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential release of a C-terminal arginine or lysine residue.</text>
            <dbReference type="EC" id="3.4.16.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.6" evidence="14"/>
    <dbReference type="EMBL" id="ADAS02000143">
        <property type="protein sequence ID" value="OAV89025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G8J2"/>
    <dbReference type="EnsemblFungi" id="PTTG_08269-t43_2">
        <property type="protein sequence ID" value="PTTG_08269-t43_2-p1"/>
        <property type="gene ID" value="PTTG_08269"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08269"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF190">
        <property type="entry name" value="PHEROMONE-PROCESSING CARBOXYPEPTIDASE KEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="418"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="512"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="457"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OAV89025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08269-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="533" mass="59936" checksum="6AC7C7FF7B7A9505" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180G9K4</accession>
    <name>A0A180G9K4_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PTTG_01267</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="22 23" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="22">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="22" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:21249"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="EMBL" id="ADAS02000133">
        <property type="protein sequence ID" value="OAV89304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180G9K4"/>
    <dbReference type="EnsemblFungi" id="PTTG_01267-t43_1">
        <property type="protein sequence ID" value="PTTG_01267-t43_1-p1"/>
        <property type="gene ID" value="PTTG_01267"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_01267"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000011">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.150:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1070.20:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000016">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000017">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000007">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
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    </dbReference>
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        <property type="entry name" value="Rpa2_dom_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06883">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
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        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="18">
        <location>
            <begin position="52"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="216"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="19">
        <location>
            <begin position="490"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase I subunit RPA2" evidence="20">
        <location>
            <begin position="596"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="15">
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            <begin position="703"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="16">
        <location>
            <begin position="1073"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00047768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF06883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OAV89304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_01267-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="1223" mass="136713" checksum="46DDC3C13661989F" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GA75</accession>
    <name>A0A180GA75_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein HGH1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_05842</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HGH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000123">
        <property type="protein sequence ID" value="OAV89606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GA75"/>
    <dbReference type="EnsemblFungi" id="PTTG_05842-t43_2">
        <property type="protein sequence ID" value="PTTG_05842-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05842"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05842"/>
    <dbReference type="OrthoDB" id="338814at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
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    <dbReference type="InterPro" id="IPR039717">
        <property type="entry name" value="Hgh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007206">
        <property type="entry name" value="Protein_HGH1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007205">
        <property type="entry name" value="Protein_HGH1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387:SF9">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF384"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein HGH1 N-terminal" evidence="2">
        <location>
            <begin position="36"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Protein HGH1 C-terminal" evidence="3">
        <location>
            <begin position="244"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV89606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05842-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="35014" checksum="8ED984E4D47332EC" modified="2016-09-07" version="1">MTAHDAFSALINLSDTTQVVEQLKDPDFLSVIFFTIIDHDSILADLACMLLSNLTKVESLVDLCLNSTIPPHASHPTINQDQPLSARLKTSPTPLMDLLIEVFTRGDQKQINPHANFDFLASVWANLSASARGRDYLVGVGPSPTATHEAPLFQLCPFTQHPSLIRRGGVISAIKNCCFATEVHEQLLSPTGFNLLPAILLPLMGPEAIDEPEEQEDFPVECQLLGPDKRRESDANLRLILVESLILLATYPIQREIMRMKKVYRIVQILHLDETSEIVQESIERLVNLLMRDESEHAVKPKPASDDEDELIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GA80</accession>
    <name>A0A180GA80_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase II transcription factor B 38 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_05850</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000123">
        <property type="protein sequence ID" value="OAV89597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GA80"/>
    <dbReference type="EnsemblFungi" id="PTTG_05850-t43_2">
        <property type="protein sequence ID" value="PTTG_05850-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05850"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05850"/>
    <dbReference type="OrthoDB" id="5963at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015877">
        <property type="entry name" value="MAT1_centre"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683:SF13">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
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    <dbReference type="Pfam" id="PF06391">
        <property type="entry name" value="MAT1"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="97"/>
            <end position="230"/>
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    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="131"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV89597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05850-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="354" mass="39939" checksum="C99AB2C6A344F93E" modified="2016-09-07" version="1">MFVQCVNLTGTSIQTSGCSSQNVTTRCANRVSIGSSRWARNRVQFVDKSSERRVSHSRRSKIWPLKRKWLSVNGSLNTSDLISFCTTLTFSLLLRCSFNKRQEDFMTLDAYNNYLEEVEDITFNLINGVDVAETEAKIKQFQIENQELIAQNAVHEARQAELSKRQEEAVRKEREERKAELLRLEEEARREEEEIKRATIHSLETSNVSADKLVARQRAVAQKRATARALASDLATKATVAMPSLSGLIDPSSMEGADVNRPDIEGELGQWDDYSSMFDLKRLDANLQPGYIDHDSMRLVLADHALALVGGFEIESVWERQLRSAMMGLFVPPPEAGAINQTTSDDLSLHNLRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
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    <name>A0A180GEA0_PUCT1</name>
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        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
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    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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    <reference evidence="3" key="1">
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000088">
        <property type="protein sequence ID" value="OAV91056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblFungi" id="PTTG_28109-t43_2">
        <property type="protein sequence ID" value="PTTG_28109-t43_2-p1"/>
        <property type="gene ID" value="PTTG_28109"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_28109"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008109828" description="Secreted protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="182"/>
        </location>
    </feature>
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        <location>
            <begin position="33"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV91056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_28109-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="19707" checksum="DE0DDF310646E2B5" modified="2016-09-07" version="1">MVSSRFISHVCLLSVLVLIAIVNGAAIPSNEIRSDGPVGWADCSEDISDDDQESDYDEPQEDASVVAISVTRIVHVDALPASFQPNNPEQEPAQGSTTTIVQVSVIGLAGSEEQDDSEPLDFESDADAEVDDETRPFSRSVEVEVGAGIDCEHDYQPREFNTASDIFQTQEASPDSMYRVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GEA8</accession>
    <name>A0A180GEA8_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nodulin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PTTG_12602</name>
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    <organism evidence="6">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000105">
        <property type="protein sequence ID" value="OAV90273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GEA8"/>
    <dbReference type="EnsemblFungi" id="PTTG_12602-t43_3">
        <property type="protein sequence ID" value="PTTG_12602-t43_3-p1"/>
        <property type="gene ID" value="PTTG_12602"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12602"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF158">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 12-LIKE CONSERVED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="345"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="413"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OAV90273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12602-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="47073" checksum="7CE293EB1FBF2EB5" modified="2016-09-07" version="1">MAQHFRQSFSVLAILANSLTAGSLFTYPIFSPSLQSQLKFSSKQVSGTASIAILSQYLSAGIWGSLADTLGSARSSEDPHLTNGSQYPHQSSQSAGFETWIQVTIAYSICGAATAASYFAAITASTRIFGEKKPGLSVAGPSSMFGLSPLLLSFLGVKFFSSPDGSFNSSGYLAFLAILTLIVNITGSIGFQRNDELNLTPSSPHSHDERTPLIQTSPPVLNHSMHSKTAATLEQDPEGLASFLASPTVWILGTVVLLSVGPAEMTIASIGAVADAKNPGLGTAFKARQVQLISITNTLSRLLSGWLSDRLCRSGWGSRLVLWGFACFCYSIACFSVAVNGGIVWVLSAVTGSCYGIVYTIAPSLVATVWPIKFFGRNYGVISYFFATGSFLFIFLFGILFESSDSNSIGRVYEISGIFELISIGLMVLLYRKYWMHGIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GEC6</accession>
    <name>A0A180GEC6_PUCT1</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_01996</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
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        <property type="gene ID" value="PTTG_01996"/>
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            <end position="223"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="412"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="333"/>
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    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="OAV90889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_01996-t43_1-p1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="670" mass="72763" checksum="2567FDA2ABCB8F28" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GED7</accession>
    <name>A0A180GED7_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Homeobox domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_07356</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TALE/M-ATYP homeobox family.</text>
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        <property type="protein sequence ID" value="OAV90303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GED7"/>
    <dbReference type="EnsemblFungi" id="PTTG_07356-t43_5">
        <property type="protein sequence ID" value="PTTG_07356-t43_5-p1"/>
        <property type="gene ID" value="PTTG_07356"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_07356"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
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    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
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    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
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    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="390"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
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            <end position="391"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
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    <feature type="region of interest" description="Disordered" evidence="6">
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    <feature type="compositionally biased region" description="Low complexity" evidence="6">
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        <source>
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    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OAV90303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_07356-t43_5-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="625" mass="67126" checksum="BE0A811DE72067EC" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GFC1</accession>
    <name>A0A180GFC1_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="12">MFS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PTTG_05678</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>myo-inositol(out) + H(+)(out) = myo-inositol(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:60364"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000081">
        <property type="protein sequence ID" value="OAV91417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GFC1"/>
    <dbReference type="EnsemblFungi" id="PTTG_05678-t43_1">
        <property type="protein sequence ID" value="PTTG_05678-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05678"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05678"/>
    <dbReference type="OrthoDB" id="2544694at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF28">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="1"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="427"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="449"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OAV91417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05678-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="458" mass="50706" checksum="6A3D536E3F401794" modified="2016-09-07" version="1">MITFVCGEALGRKMTIRIGAIGMSGGALLQTLALSRECLTIGRVLSGIGMGFISSTAPVLLAEASPPESRGRHACAQLSTLNFGICLSYWMNYCTTMRLTGSVTFQLPIAFQYSMIIPIAVLTCLVPESPQWLMFHNRAPEALASLRLLEGAQPDEIALRSRFLEMEANAIYAHSIEDIGYKALFQNDGLRTRERILIACSVQCFQQLGGVNVIVYHAHFMFSSCLGFSEQATGLISALLFTWFLIASFIPWFLIDTFGRRLLMIPSVAIMGSIFAALAGVAKQIEENENSHQSRMAAVVLFFVFLAVFAIGFQATVWFYPPEILPQRVRAQGAALATACNWSLNFVVVECFPLATEKIGWKTYLIFMALNISFVPIMFIFYPETKGKSLEEIDLMFGDAVSAELRRQKMNEIQTSLHGIDALGEEGRKDLDHKRKSNHPPYRSSSTPEGEKACDNIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GFC8</accession>
    <name>A0A180GFC8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_06797</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000083">
        <property type="protein sequence ID" value="OAV91284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GFC8"/>
    <dbReference type="EnsemblFungi" id="PTTG_06797-t43_2">
        <property type="protein sequence ID" value="PTTG_06797-t43_2-p1"/>
        <property type="gene ID" value="PTTG_06797"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06797"/>
    <dbReference type="OrthoDB" id="5568181at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV91284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06797-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="240" mass="26740" checksum="BB7B42E517F95143" modified="2016-09-07" version="1">MRSRSQLETSPLSPNALSYPTLISKFLILLNHTEALSQALNIPTPSRPVDRFELPSVRAEREEREKWEHGPAKLSVLGNLAAVPRGLEGLEDGKEWIVGVLTRTKLAPEIEKDEQDLLSLLPPPPENDSIPTIGLLLEHVKSLTKAHEKHIANCRTALHKARYGRATEPMDIDISSNNGNADEGDAKYEWKMRVEVDDQLKGSAKAAPTEISPAQNMMLKNLLGFMRDGRKFSSHLEKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GFE8</accession>
    <name>A0A180GFE8_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mitochondrial fission process protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Mitochondrial 18 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_05110</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MTFP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000080">
        <property type="protein sequence ID" value="OAV91446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GFE8"/>
    <dbReference type="EnsemblFungi" id="PTTG_05110-t43_2">
        <property type="protein sequence ID" value="PTTG_05110-t43_2-p1"/>
        <property type="gene ID" value="PTTG_05110"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05110"/>
    <dbReference type="OrthoDB" id="424969at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019560">
        <property type="entry name" value="Mitochondrial_18_kDa_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11001">
        <property type="entry name" value="MITOCHONDRIAL FISSION PROCESS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11001:SF2">
        <property type="entry name" value="MITOCHONDRIAL FISSION PROCESS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10558">
        <property type="entry name" value="MTP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV91446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05110-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="127" mass="13810" checksum="D18DBD26AF8D8ABB" modified="2016-09-07" version="1">MPDNVENLPERIADGRIDSTDTAARYLGLVARIRPLLIPASRYLAYTSDVGEAFRPVVNPKVVTGAYGISIAYVVGDVAYEGWKGHLQAQGDPNDNLVIGLKVARRAAFQGTASMLFPALVRKFCFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GGC8</accession>
    <name>A0A180GGC8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ENDO3c domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_04793</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000089">
        <property type="protein sequence ID" value="OAV91023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GGC8"/>
    <dbReference type="EnsemblFungi" id="PTTG_04793-t43_2">
        <property type="protein sequence ID" value="PTTG_04793-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04793"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04793"/>
    <dbReference type="OrthoDB" id="5607at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000702">
        <property type="term" value="F:oxidized base lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47203">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47203:SF1">
        <property type="entry name" value="HYPOTHETICAL BASE EXCISION DNA REPAIR PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="2">
        <location>
            <begin position="132"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV91023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04793-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="289" mass="31489" checksum="8CA3EC6E1D157EEF" modified="2016-09-07" version="1">MSPQAHKQIIILSHNVSFQLSNHHHSPMTSEYRGTDYSHPVALRHEPYKSARSEAMEKPVLACSSERHISKPTSSDCRQVCSLLAGVHGGMPARPERLMGATEKGDRGSSRTLATECGEVGEVLDGLVRTILSQHTSQSNSIRSKQALDEHFGTGNYHAIRLASVSAITAILQNARVGLAAKKSSTIHALLNHIYLNLNPELSLEFLRCLSDSEAMEILTSFKGVGAKTASCVMLFCLGRNFFPVDTHVFRITKALGWLPPSATRESAFKHLDQAVPDQLNQSSPKGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GGF5</accession>
    <name>A0A180GGF5_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="6">PPIase</shortName>
            <ecNumber evidence="6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_07556</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 6">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyclophilin-type PPIase family. PPIL3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="6"/>
    <dbReference type="EMBL" id="ADAS02000074">
        <property type="protein sequence ID" value="OAV91826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GGF5"/>
    <dbReference type="EnsemblFungi" id="PTTG_07556-t43_1">
        <property type="protein sequence ID" value="PTTG_07556-t43_1-p1"/>
        <property type="gene ID" value="PTTG_07556"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_07556"/>
    <dbReference type="OrthoDB" id="271386at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000012">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF2">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="7">
        <location>
            <begin position="5"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OAV91826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_07556-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="146" mass="16345" checksum="81F6AC36F9EBF558" modified="2016-09-07" version="1">MFLYRNFLALCASGYYDNCIWHRNIKGFMIQTGDPTGTGKGGQSIWGRPFADEIRSTLKFNQRGIVAMASSGRDTNKAQFFILYAKQPHLDGHYTIIGRVIDGAINGTLDLMEKVAVNEKHRPVQEIKMTGVTIHANPIADKEAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GIF8</accession>
    <name>A0A180GIF8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_09570</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000063">
        <property type="protein sequence ID" value="OAV92470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GIF8"/>
    <dbReference type="EnsemblFungi" id="PTTG_09570-t43_2">
        <property type="protein sequence ID" value="PTTG_09570-t43_2-p1"/>
        <property type="gene ID" value="PTTG_09570"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09570"/>
    <dbReference type="OrthoDB" id="2500136at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV92470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09570-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="293" mass="33761" checksum="049EC39816768D1D" modified="2016-09-07" version="1">MNTSSQLPPADLIPRLEGYLKNLQSLAQAISTVRYQTFPSLINNLQQPIQACDPNNPSSSPDHLNEVKENWKCDSQDLLQGLKALRLQAMNSQEAMKYVRATTQTNMHLTIEEKEKAFEETRLLNQKTKEDGLQRFKSRSSLSRRAKATSPIIEQHIIETELLKRKKLRLLKQRCSFPEIFVPVIDLKDCIQRINASDRQSGHLTFVSENQILLKDVMKIFVVFKRTTQSHKLGPSLVIERAFCFGLTESPTNTFQRSSFGLIRSINRCINHYINRFFNTNHDQNASNLWLIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GII6</accession>
    <name>A0A180GII6_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_03687</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000068">
        <property type="protein sequence ID" value="OAV92142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GII6"/>
    <dbReference type="EnsemblFungi" id="PTTG_03687-t43_1">
        <property type="protein sequence ID" value="PTTG_03687-t43_1-p1"/>
        <property type="gene ID" value="PTTG_03687"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03687"/>
    <dbReference type="OrthoDB" id="2507853at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="170"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV92142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03687-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="441" mass="46445" checksum="ACA80C5DFD895A7E" modified="2016-09-07" version="1">MPALTAYAHAPAHGAGWPSMNSSNKPSLVPRTGSSCSQSALAPGASPPITARPRSTKSRFERLAKPLSSPASRLRKSKSTNSQLHQQQQQPVLLVTPSPPNAVRGILTKHSSLLNLSSRISTAVLERNPAPLVIGQPSAPPPTSAQPVTKKVRFSGNLGDGGARAQERMGSSSSAPSGCGCARHCRGQTPTEGAEACRQLEPVIETIFLTYSRSAYDRSPIAVDRVFNKSLALPPRTEEDSDCGSGRWLADLLPVSAASPHTLLLPAGPVERGFPSELGSDQHLEAKILLPLATLEDPASADPLDAHHIYVATESPEAVDWPSRPEIFGHSWFSSEDARSESTASSSSSQEPNTPAIAALFPHAPLNALLAPIPVSVAPSDQACSLSVENLAHHAFENLSLNGSDLPFPPACVSLDADPLCGFGNWTRGQVFDSCDALDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GII8</accession>
    <name>A0A180GII8_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cystathionine beta-synthase</fullName>
            <ecNumber evidence="4">4.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_04494</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-homocysteine + L-serine = L,L-cystathionine + H2O</text>
            <dbReference type="Rhea" id="RHEA:10112"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="4.2.1.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.22" evidence="4"/>
    <dbReference type="EMBL" id="ADAS02000062">
        <property type="protein sequence ID" value="OAV92576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GII8"/>
    <dbReference type="EnsemblFungi" id="PTTG_04494-t43_2">
        <property type="protein sequence ID" value="PTTG_04494-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04494"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04494"/>
    <dbReference type="OrthoDB" id="10259545at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004122">
        <property type="term" value="F:cystathionine beta-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000003">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000118">
        <property type="entry name" value="Related to CYS4-cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="7">
        <location>
            <begin position="50"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OAV92576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_04494-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="408" mass="44023" checksum="E5648E7A23AC56C6" modified="2016-09-07" version="1">MAHGELLRNGNSTIASALAPEPVAKNPERDDPIMPRIDNSVLSISDSVLSCIGATPLIRLDRLAKKYNLKCNLLAKLEYYNAGGSVKDRIALRMVVEAEREGILTPGKSVIIEPTSGNTGIGLALAAAVKNYRCIIVMPQKMSREKINMMKALGTEIVRTPTEAAFDSYESHLSVAARLEKAIPGGVILNQYGNRFNPQTHYDTTGLEIIQAIQNTPSTKSRPSSGIVDLFIAGAGTGGTISGVSRRLKKHYGAERVKSIAVDPVGSILAKPESLNEAGVQNYQTEGTGYDFEPEVLDYSVIDQWIKVSDPEAFQTLREMIKLEGTLCGGSSGQAVYGALNYLKPANQDDGSSPGEGWEKYGKNSDVNVVVLCADSIRNYITKDWLIEGTEDSETTEAALVSQAQNQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GIJ6</accession>
    <name>A0A180GIJ6_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_27607</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000062">
        <property type="protein sequence ID" value="OAV92587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GIJ6"/>
    <dbReference type="EnsemblFungi" id="PTTG_27607-t43_1">
        <property type="protein sequence ID" value="PTTG_27607-t43_1-p1"/>
        <property type="gene ID" value="PTTG_27607"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_27607"/>
    <dbReference type="OrthoDB" id="10632435at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008109940" evidence="2">
        <location>
            <begin position="29"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV92587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_27607-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="151" mass="16281" checksum="40BD3596F98726BD" modified="2016-09-07" version="1">MLRMLVHPGELALFGLLWILIAETPTHCSRIGVWKRALDATELRAVSLIQKTKGTVEAAHGTGAPKEERKTPSSLMTQNSQEKNGFNFASPTGPGKQPVFAQGDDATDNQDKNKNLAALRETEAIATKIGLKSSYVQLASGIAEKGKLLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GIJ7</accession>
    <name>A0A180GIJ7_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Importin N-terminal domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PTTG_27677</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="12">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000066">
        <property type="protein sequence ID" value="OAV92268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GIJ7"/>
    <dbReference type="EnsemblFungi" id="PTTG_27677-t43_2">
        <property type="protein sequence ID" value="PTTG_27677-t43_2-p1"/>
        <property type="gene ID" value="PTTG_27677"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_27677"/>
    <dbReference type="OrthoDB" id="7862313at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="9">
        <location>
            <begin position="28"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="8">
        <location>
            <begin position="170"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="8">
        <location>
            <begin position="391"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OAV92268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_27677-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="765" mass="84232" checksum="6A233F541B0DE20A" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GIK0</accession>
    <name>A0A180GIK0_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MFS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_03492</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000063">
        <property type="protein sequence ID" value="OAV92530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="ADAS02000063">
        <property type="protein sequence ID" value="OAV92531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GIK0"/>
    <dbReference type="EnsemblFungi" id="PTTG_03492-t43_1">
        <property type="protein sequence ID" value="PTTG_03492-t43_1-p1"/>
        <property type="gene ID" value="PTTG_03492"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_03492-t43_2">
        <property type="protein sequence ID" value="PTTG_03492-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03492"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03492"/>
    <dbReference type="OrthoDB" id="419616at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17330">
        <property type="entry name" value="MFS_SLC46_TetA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF15">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
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                <person name="Goldberg J."/>
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                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
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                <person name="White J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
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                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
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                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
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    <reference evidence="3" key="4">
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
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                <person name="Feldgarden M."/>
                <person name="Earl A."/>
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                <person name="Zeng Q."/>
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                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
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                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
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                <person name="Baranova O.V."/>
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                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
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    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Khalil H.B."/>
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                <person name="Joly D."/>
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                <person name="Sakthikumar S."/>
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                <person name="Adiconis X."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
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            <end position="142"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV92629.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05004-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="15711" checksum="5787D45E9D2D49FC" modified="2016-09-07" version="1">MKTFLVLMVAMIVSGPAFARKLAPVIVSPKVKKVLLDDDYKVAVEEEAINPIKACLRYDKDLRPTIPELLKDEFLLGSLAPTAARSTVNADGGMTLQPGMFELVIHKSWDWYARKTGNGGRLTEAQKKKFLASLKMSVKCSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GJN1</accession>
    <name>A0A180GJN1_PUCT1</name>
    <protein>
        <submittedName>
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                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
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                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
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                <person name="Engels R."/>
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                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
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                <person name="Saif S."/>
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                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
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        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
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        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
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            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
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                <consortium name="EnsemblFungi"/>
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        </citation>
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            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
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        <property type="gene ID" value="PTTG_04987"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04987"/>
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            <position position="1"/>
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            <dbReference type="Proteomes" id="UP000005240"/>
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    </evidence>
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        <name evidence="11" type="ORF">PTTG_27520</name>
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        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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            <taxon>Pucciniomycetes</taxon>
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    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
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                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
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        <text evidence="3">Belongs to the sirtuin family. Class I subfamily.</text>
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        <property type="gene ID" value="PTTG_27520"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
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        <property type="entry name" value="Sirtuin"/>
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        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
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        <property type="entry name" value="Ssirtuin_cat_dom"/>
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        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACETYLASE SIRTUIN-1"/>
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        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
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            <end position="444"/>
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            <position position="265"/>
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        <ligand>
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        <ligand>
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        <ligand>
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            <dbReference type="EMBL" id="OAV92885.1"/>
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        <source>
            <dbReference type="EnsemblFungi" id="PTTG_27520-t43_2-p1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        <name evidence="3" type="ORF">PTTG_00913</name>
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        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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            <strain evidence="3">1-1 BBBD Race 1</strain>
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    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000052">
        <property type="protein sequence ID" value="OAV93307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GKJ9"/>
    <dbReference type="EnsemblFungi" id="PTTG_00913-t43_2">
        <property type="protein sequence ID" value="PTTG_00913-t43_2-p1"/>
        <property type="gene ID" value="PTTG_00913"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00913"/>
    <dbReference type="OrthoDB" id="76215at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47072">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47072:SF4">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="148"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV93307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00913-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="423" mass="46704" checksum="D7C8EA5FEAE757CD" modified="2016-09-07" version="1">MTVSGGSGDRFQNPAAAESILASGQLPSVLFRHTSPHLSPLAMSHTDPLLFPVDPRLFGSTSTSPKPTGQAAHPPETQPFAPPSSIKPSQKKRPSETLKTPQSQSKKTSASTMSFKERKKAASESDHDESAPEESENEETPSKKRYHHWTNDQKISLLTFIINQIQLGWGKPTNNGNIKDEGWIQVTKEMNKRFGVVFTLDQLKNQKGSLRKLYIDQKFLLAHSAFGWDDIARMVTADDQTWTAFIKAHPKRKFVRLRTNPIPWSDLAEQLFGRTISATGYTPSRPEEVPPQAAQFERVSTDQSPASINCRNSKKFYKMQDTPTSDNARLETVPQTITCTASPNRSVSPNTGVSVKDEGPSAKVSVRGEALKLLSSMFLDHISTEEYVRFVKVLQSESNAELFISLASTSNASVCKAWLAASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GKL1</accession>
    <name>A0A180GKL1_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc finger PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_10352</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000052">
        <property type="protein sequence ID" value="OAV93317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GKL1"/>
    <dbReference type="EnsemblFungi" id="PTTG_10352-t43_2">
        <property type="protein sequence ID" value="PTTG_10352-t43_2-p1"/>
        <property type="gene ID" value="PTTG_10352"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_10352"/>
    <dbReference type="OrthoDB" id="79252at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053051">
        <property type="entry name" value="HDAC_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47793">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT CTI6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47793:SF1">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT CTI6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV93317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_10352-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="446" mass="49271" checksum="6E654BE072CA0AAA" modified="2016-09-07" version="1">MFQCDKCSVWQHGPCVGLYAEFPGDYFCEKCRPDLHTTGQYQKSTIHQPSRRSPSFTSSSSRRERVNPDAASLAAFLTDAGVKPEDQLSLGGFALPTFAQTRRQSNMLVDGVAPLVQKKQSAVDTSSSGTTESILPSALNDPHKTKRKRPSLDQIESPTLPSVEPTVEDKLKRPRISPAAELPSPAASVSTTTSKLYNPPSASSLFSGPIGKHKRGRKVDDTDESGIVTSNNKSKAAGVFANRNPVPVSTSSTGKRGSPTKRSKEDGRRTKQQQITEQQQAQQQQQQRQQQVEALLSEGWGLPDHLSYLDYLLPSPFPQPLVIEGLGDGPQSKIFETPAKVKFPSKRTTIADLRKRSKHLVDYLQKVQIESSDREKRNELIAKSLSSKLSQPPADNSNNPQDQSHHNPPPVDSFATVQSVVSPKTLEIIDDLSRQLYHWQEKFKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GKM7</accession>
    <name>A0A180GKM7_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BZIP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PTTG_03490</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000063">
        <property type="protein sequence ID" value="OAV92523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GKM7"/>
    <dbReference type="EnsemblFungi" id="PTTG_03490-t43_2">
        <property type="protein sequence ID" value="PTTG_03490-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03490"/>
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    <dbReference type="OrthoDB" id="295274at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR021755">
        <property type="entry name" value="TF_Aft1_HRA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19304">
        <property type="entry name" value="CYCLIC-AMP RESPONSE ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11786">
        <property type="entry name" value="Aft1_HRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="815"/>
            <end position="878"/>
        </location>
    </feature>
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            <begin position="50"/>
            <end position="120"/>
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            <end position="161"/>
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            <end position="228"/>
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            <end position="484"/>
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            <end position="77"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
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    <sequence length="971" mass="101950" checksum="28EC590135320F1C" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GKP8</accession>
    <name>A0A180GKP8_PUCT1</name>
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        <recommendedName>
            <fullName evidence="3">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="3">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PTTG_02834</name>
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        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
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    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="PTTG_02834-t43_1-p1"/>
        <property type="gene ID" value="PTTG_02834"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02834"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
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        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="5">
        <location>
            <begin position="1"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OAV93039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02834-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="427" mass="48033" checksum="B9A3A883EB8939F8" modified="2016-09-07" version="1">MNYRLGSFGFLNSDDLPVEDLQVGLKDQIACLKWLKLNIKAFGGDPKKITLWGQSAGAIAVSTLVTYLYDSPSSTLFRAAIMDSGSPTSHTVPAVHVYDRPGMPYNLLVNGTGCDSPTTVKGARRSRKSRKSQKSRSAQVDRLECLRELDYNVLLNASLALLRTPPFSRQVTVWGPSYKAGSIIDRRPSERLADDDFIKIPMILGSNKDEGALAAVGPSIAFRGPPEDSDAYFYSYMSNSSVLDLQQVDKQVYEKVAELYPDEPSLGSPFDTGNRTFGLPRIFKRLAAWYGDLHYQAPKRFWARKASPHQPVYVYYFDGPPASNDHAYGGVSHSSELPLIFGNLDEEDLSITEVKEYRALAERVRNRYISFVYELNPGDDWPAYTPRAKKVMRFNKKIIEGELIEDDWRAEQLEYLNSEPAQNTYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GL25</accession>
    <name>A0A180GL25_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_05576</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000053">
        <property type="protein sequence ID" value="OAV93244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GL25"/>
    <dbReference type="EnsemblFungi" id="PTTG_05576-t43_1">
        <property type="protein sequence ID" value="PTTG_05576-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05576"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05576"/>
    <dbReference type="OrthoDB" id="2501173at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV93244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05576-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="546" mass="58813" checksum="1D8AED045B126F87" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GLL5</accession>
    <name>A0A180GLL5_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="8">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PTTG_09343</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="8"/>
    <dbReference type="EMBL" id="ADAS02000051">
        <property type="protein sequence ID" value="OAV93369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GLL5"/>
    <dbReference type="EnsemblFungi" id="PTTG_09343-t43_3">
        <property type="protein sequence ID" value="PTTG_09343-t43_3-p1"/>
        <property type="gene ID" value="PTTG_09343"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09343"/>
    <dbReference type="OrthoDB" id="17199at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000003">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039799">
        <property type="entry name" value="ALR/ERV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645">
        <property type="entry name" value="ALR/ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645:SF0">
        <property type="entry name" value="FAD-LINKED SULFHYDRYL OXIDASE ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="86"/>
            <end position="187"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OAV93369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09343-t43_3-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="7 8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_01586</name>
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    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM170 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000051">
        <property type="protein sequence ID" value="OAV93418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="ADAS02000051">
        <property type="protein sequence ID" value="OAV93419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GLR8"/>
    <dbReference type="EnsemblFungi" id="PTTG_01586-t43_2">
        <property type="protein sequence ID" value="PTTG_01586-t43_2-p1"/>
        <property type="gene ID" value="PTTG_01586"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="PTTG_01586-t43_3">
        <property type="protein sequence ID" value="PTTG_01586-t43_3-p1"/>
        <property type="gene ID" value="PTTG_01586"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_01586"/>
    <dbReference type="OrthoDB" id="2131401at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019334">
        <property type="entry name" value="TMEM170A/B/YPR153W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22779">
        <property type="entry name" value="SD17342P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22779:SF6">
        <property type="entry name" value="SD17342P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10190">
        <property type="entry name" value="Tmemb_170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV93418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_01586-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="153" mass="16874" checksum="7769C2C029955A63" modified="2016-09-07" version="1">MGCGLFASFTFWRSGSRAVNSSKSDNMVKMGLGGVTKKTKIRRGSAGSIHNQEEWIPLNRHRRASSIITTIHHPTIHLNRTRRPFASLFITPVFFLVIYSFSAVISGSLVGWAIAALYNAAFLRMSTWVPALWSAGLTLIVIISSYSNISTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GM06</accession>
    <name>A0A180GM06_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycoside hydrolase family 5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_06583</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000048">
        <property type="protein sequence ID" value="OAV93685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GM06"/>
    <dbReference type="EnsemblFungi" id="PTTG_06583-t43_1">
        <property type="protein sequence ID" value="PTTG_06583-t43_1-p1"/>
        <property type="gene ID" value="PTTG_06583"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_06583"/>
    <dbReference type="OrthoDB" id="9971853at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050295">
        <property type="term" value="F:steryl-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904462">
        <property type="term" value="P:ergosteryl 3-beta-D-glucoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000091">
        <property type="entry name" value="Chromosome 1, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000122">
        <property type="entry name" value="Glycoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000106">
        <property type="entry name" value="Glycosyl hydrolase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041036">
        <property type="entry name" value="GH5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052066">
        <property type="entry name" value="Glycosphingolipid_Hydrolases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308:SF6">
        <property type="entry name" value="GLYCOSIDE HYDROLASE FAMILY 5 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18564">
        <property type="entry name" value="Glyco_hydro_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="5">
        <location>
            <begin position="140"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5 C-terminal" evidence="6">
        <location>
            <begin position="742"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="66"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="805"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV93685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_06583-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="837" mass="93086" checksum="913D4DE01DE8DD90" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GM12</accession>
    <name>A0A180GM12_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuole protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_02984</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000049">
        <property type="protein sequence ID" value="OAV93611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GM12"/>
    <dbReference type="EnsemblFungi" id="PTTG_02984-t43_2">
        <property type="protein sequence ID" value="PTTG_02984-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02984"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02984"/>
    <dbReference type="OrthoDB" id="2128042at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031606">
        <property type="entry name" value="Kch1/2"/>
    </dbReference>
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        <property type="entry name" value="LOW AFFINITY K(+) TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR36424">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16944">
        <property type="entry name" value="KCH"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV93611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02984-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="45279" checksum="DDE97AF7D59A39BF" modified="2016-09-07" version="1">MCCTGAQWKREEVPDHKWDFVDVREFHNSGCMSYFQYCFLYVLVIKSVLVYASDLYTMVLLLASNHWAGSILESSGGSSAMNVPFKIGKWIFFGCIIFSFLLLAWEAKKSRAIIRSRDISYAFTNVMANNYYSIKSYDHFCFFSQINNSKKKKDDFAFFVFFTFKGWKRLLLADAPRQVINGITLYSFARSVGFTTDISKWYDGSFAKASVLISMAFTVTIWLGSFILLMAAAFLYVPLLCYIQGNLKEYCCHKVDKRIAELMKRKNRKRLAKEAALMRKEAMGDYSHLKDKKGQFKAAPLPQPTLPQIGLHELISDNKSERGGGAGSIRNVSRVAQHAYPPSLGHHYASSNPGTGYAPSNPGTAVGMGYNVMNRWPSDREYNEKAGYAESTASPYFHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GM21</accession>
    <name>A0A180GM21_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="12">TRP_N domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PTTG_02978</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor potential (TRP) ion channel family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000049">
        <property type="protein sequence ID" value="OAV93621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GM21"/>
    <dbReference type="EnsemblFungi" id="PTTG_02978-t43_2">
        <property type="protein sequence ID" value="PTTG_02978-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02978"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02978"/>
    <dbReference type="OrthoDB" id="2115177at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032800">
        <property type="entry name" value="TRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF2">
        <property type="entry name" value="FLAVIN CARRIER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14558">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01320">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008110049" evidence="9">
        <location>
            <begin position="18"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="376"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="460"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="507"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="533"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="563"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="594"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="ML-like" evidence="10">
        <location>
            <begin position="20"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="639"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="639"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="710"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OAV93621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02978-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="719" mass="78544" checksum="F181C0C7DB5242D5" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GMN7</accession>
    <name>A0A180GMN7_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GPI-anchor transamidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_08596</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C13 family.</text>
    </comment>
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        <property type="protein sequence ID" value="OAV93935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GMN7"/>
    <dbReference type="EnsemblFungi" id="PTTG_08596-t43_2">
        <property type="protein sequence ID" value="PTTG_08596-t43_2-p1"/>
        <property type="gene ID" value="PTTG_08596"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PTTG_08596"/>
    <dbReference type="OrthoDB" id="192611at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003923">
        <property type="term" value="F:GPI-anchor transamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000021">
        <property type="entry name" value="GPI-anchor transamidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028361">
        <property type="entry name" value="GPI_transamidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001096">
        <property type="entry name" value="Peptidase_C13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067:SF1">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01650">
        <property type="entry name" value="Peptidase_C13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00776">
        <property type="entry name" value="HEMOGLOBNASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OAV93935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_08596-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="322" mass="35667" checksum="7AF090EB9549EB7E" modified="2016-09-07" version="1">MYRSVKRLGIPDSNIILMLADDMACNPRNMFPGTVYSNSERKLDLYGDGIEVDYRGEEVSVENFIRLLTGRVAEGTPRSKRLMTDERSNILVYMTGHGGDEFLKFQDAEEISAFDLADAFQTMWAKKRYNELLFMIDTCQANTMTSKLYSPNIVATGSSAKGENSYSHHADSDIGVAVVDRFSHFVLSHLEGMNKTSSSTLQDFVNAYSYDIFHSNPGARSDLLGRSMSEVLMTDFFGGVSSVELAALPADSPVGSAAEPTKPEAIWKLTTSRWNSARRTADDSFAGPGRSLQGHANWRKLSPTLLLTLTFAAHFWAAHKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GMR3</accession>
    <name>A0A180GMR3_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PTTG_27089</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000043">
        <property type="protein sequence ID" value="OAV94086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GMR3"/>
    <dbReference type="EnsemblFungi" id="PTTG_27089-t43_2">
        <property type="protein sequence ID" value="PTTG_27089-t43_2-p1"/>
        <property type="gene ID" value="PTTG_27089"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_27089"/>
    <dbReference type="OrthoDB" id="2507721at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="581"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OAV94086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_27089-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="605" mass="68253" checksum="A7206E23A7D53018" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GMR6</accession>
    <name>A0A180GMR6_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_02995</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000049">
        <property type="protein sequence ID" value="OAV93592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GMR6"/>
    <dbReference type="EnsemblFungi" id="PTTG_02995-t43_2">
        <property type="protein sequence ID" value="PTTG_02995-t43_2-p1"/>
        <property type="gene ID" value="PTTG_02995"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02995"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF111">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007950405" description="Transmembrane 9 superfamily member" evidence="7">
        <location>
            <begin position="23"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="430"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="474"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OAV93592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02995-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="532" mass="59525" checksum="B4482687754381C5" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GN14</accession>
    <name>A0A180GN14_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PTTG_02870</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000043">
        <property type="protein sequence ID" value="OAV94065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GN14"/>
    <dbReference type="EnsemblFungi" id="PTTG_02870-t43_1">
        <property type="protein sequence ID" value="PTTG_02870-t43_1-p1"/>
        <property type="gene ID" value="PTTG_02870"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02870"/>
    <dbReference type="OrthoDB" id="10054429at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF9">
        <property type="entry name" value="AMINO-ACID PERMEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV94065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02870-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="49587" checksum="9031E29B1D37720E" modified="2016-09-07" version="1">MPFLFNTPMLSGGPASVVWCWLLGSVMCFGFGTSIAELVSAYPTSGGLYSASAYLVPRRYRPFVGFTVGWLNILGQIAGVASTEFALSEMIWAAYTLMRNDDFSPSKSQTVGLYVGLLVLHGLLNCLATKALAGITKSFIFINLTGTMAMIIGLLATTPDKHDATYIFTKVVDQTGWGNDGLAFLLGLLSVQWTMTDYDATAHISEEVKRAAIAAPVAIFVAVAGTGLFGFVLNIVLVMCSGNIDINNRGGRSEMIVANILYRNLGPTWFAIVWAVICLNSFSVVLTALQANSRTIFSFSRDGGLPDRGIFSRLSVQKVPVYAVWSVIVVSILMGLLKFASTVALNAIFSLCTIALDSSYAIPIAMKLIYMDHPEVQFKPGPFSLGRGTPLMWFVNLLSLAWVSFVVVILALPTVIPVTVLNMNYASVITFIVISLSTIWYLTSARHWYVGPKSNLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GP58</accession>
    <name>A0A180GP58_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_03031</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000039">
        <property type="protein sequence ID" value="OAV94500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GP58"/>
    <dbReference type="EnsemblFungi" id="PTTG_03031-t43_2">
        <property type="protein sequence ID" value="PTTG_03031-t43_2-p1"/>
        <property type="gene ID" value="PTTG_03031"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03031"/>
    <dbReference type="OrthoDB" id="2495649at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV94500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03031-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="76" mass="8650" checksum="172306C268B65D8C" modified="2016-09-07" version="1">MCSCFGLWPVVTNTSFSKRFRQAIWQIHNANIAAGAEYMRILKIEQDKARAEGRVPPERLDKPASFYNSNDSLSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GP70</accession>
    <name>A0A180GP70_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mid2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_27093</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000043">
        <property type="protein sequence ID" value="OAV94102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GP70"/>
    <dbReference type="EnsemblFungi" id="PTTG_27093-t43_1">
        <property type="protein sequence ID" value="PTTG_27093-t43_1-p1"/>
        <property type="gene ID" value="PTTG_27093"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_27093"/>
    <dbReference type="OrthoDB" id="2506631at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008110131" description="Mid2 domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV94102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_27093-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="430" mass="45089" checksum="9B9300EA15F3A127" modified="2016-09-07" version="1">MPAPTTLVLLLLLALASQPIHAQAILNPASPASPAPAAPAASPSPAPTSPSPPPAEANPPAPPEPSQSSTTNAAPAAPTSSPVPANNSPASSSVQSANSQTNSVASQSSASSLASRPSSTSSTAPQTISGGLSQPASSIVIVTLTRTGADGQIYTSLTSTYSPLARPSSTPNNNGSASGSSSNTWAIIGGIVGGLAGIAAVVFIVFRCTQRRFSDLDDEDVAIKWPELVNRAEDPSTLNPLAARPGVGHGIGEDDDQTDEKPRLYSHDIAMESLHSDQRHPGMYDSYQAYPPPPPLHPQEHNGYYDPYLGPSAAQQPYPGTLNNLGYDDQPGYQHRPHDLHSQPSLGSSVGNTHDPQGPPRPDSRQQQQQQQQQLGHLNLSDHHLQYPIAWDGPEDIPLTVVNNNSHHQQQQQQQPSANSAPLHNPYHPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GP90</accession>
    <name>A0A180GP90_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Chitin synthase</fullName>
            <ecNumber evidence="2 7">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_04292</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the chitin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2 7"/>
    <dbReference type="EMBL" id="ADAS02000043">
        <property type="protein sequence ID" value="OAV94122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GP90"/>
    <dbReference type="EnsemblFungi" id="PTTG_04292-t43_2">
        <property type="protein sequence ID" value="PTTG_04292-t43_2-p1"/>
        <property type="gene ID" value="PTTG_04292"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_04292"/>
    <dbReference type="OrthoDB" id="26569at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    <reference evidence="9" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
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                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
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            <strain evidence="9">1-1 BBBD Race 1</strain>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
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                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
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                <person name="Adiconis X."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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            <strain evidence="11">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
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                <consortium name="EnsemblFungi"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="10">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
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        <property type="gene ID" value="PTTG_00614"/>
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        <property type="entry name" value="Amino_acid_permease_CS"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
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</entry>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">1-1 BBBD Race 1</strain>
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    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
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    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="EnsemblFungi"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000039">
        <property type="protein sequence ID" value="OAV94468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GPV7"/>
    <dbReference type="EnsemblFungi" id="PTTG_26969-t43_1">
        <property type="protein sequence ID" value="PTTG_26969-t43_1-p1"/>
        <property type="gene ID" value="PTTG_26969"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_26969"/>
    <dbReference type="OrthoDB" id="417450at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039773">
        <property type="entry name" value="BAG_chaperone_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329:SF16">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329">
        <property type="entry name" value="BCL2-ASSOCIATED ATHANOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAG" evidence="3">
        <location>
            <begin position="147"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV94468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_26969-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="243" mass="26823" checksum="E934C03A6C0EC974" modified="2016-09-07" version="1">MEVIWDSERFDVDLPVSSFTLGSFKRYLTSRTGVPAEHMKLFCNAGLMKDDSAPLSAFQSQVDSQLDPGAQSSSPLSRSRLWNGIFSGTKKRQVPSMRIRMVGTKETRSIVSDRPDLKPTAPSSQNLKPQAGSNLEIMNESRIISLINELTQKTLSDNLPRVERYEGMFPTDSDEVSGDGPDPQTTKRVQDSTEFAGVSEVLLQALLKLDGFEIESEWAEARMARKEGVRAVQKLLGATGPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GPX9</accession>
    <name>A0A180GPX9_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PTTG_12035</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000040">
        <property type="protein sequence ID" value="OAV94352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GPX9"/>
    <dbReference type="EnsemblFungi" id="PTTG_12035-t43_2">
        <property type="protein sequence ID" value="PTTG_12035-t43_2-p1"/>
        <property type="gene ID" value="PTTG_12035"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12035"/>
    <dbReference type="OrthoDB" id="10289122at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV94352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12035-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="389" mass="44528" checksum="88D04C99CE40D974" modified="2016-09-07" version="1">MPQEDPVLVGFDRLIKKHEPLYSEPWTFGINSLDGAFSALSLDQVRAHKKLLDWMHRSLLPRLNEQIRTLAILLLPSDYWKNPKETRKLILQVQSELDNTTDAIKSIITSICPTSTGLAQERTDDQHLKGLKSFRLQLLKPRIHNDLLYHINKVFSLAHELFQQIKLAPDAVKPDKFDGCSYRNDFIKDAWITFRGIDLTIKFMESSELDLAQEQWVFQFAQLDKTIRGIVRTANQLQSKQINNPKNLQVRKAAELARIAIPMTKLIKMFFIKISKEGINQRRFPTYTKMSSQQIQSLTHSLDTSGEDLLALQSLLSRIGGSGFGAANSREAMQIANNLKSRAETTLSLVLLYCVPLIKNTDGFESREYYKTWFDTWSAAFDLATPEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GPY7</accession>
    <name>A0A180GPY7_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="6">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_03801</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="6"/>
    <dbReference type="EMBL" id="ADAS02000038">
        <property type="protein sequence ID" value="OAV94584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GPY7"/>
    <dbReference type="EnsemblFungi" id="PTTG_03801-t43_3">
        <property type="protein sequence ID" value="PTTG_03801-t43_3-p1"/>
        <property type="gene ID" value="PTTG_03801"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03801"/>
    <dbReference type="OrthoDB" id="3264224at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF20">
        <property type="entry name" value="[3-METHYL-2-OXOBUTANOATE DEHYDROGENASE [LIPOAMIDE]] KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="EMBL" id="OAV94584.1"/>
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        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03801-t43_3-p1"/>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="265" mass="29859" checksum="BB9E39B9F4B20A6F" modified="2016-09-07" version="1">MARSDFHLKLWSTVSPHNGHRLRLPVKPRRPITTFTKRHIPHTVSSTDYAHSLAGQSLKRVSLDRLMRFGPPPLSPSKIVESAELTRYELSQRLQRRVNAHLSLPYLPASNPHIKEVMSIYRHSLEEINSLPPIRTVADNAALLQALVTMVDDAADVIGMFATGFKESKRYLSEEQITSFLDRAIQSRISIRLIAEQHLSLSKAEHSPNGSRLGIVDRKVKLKKTLESVLEFAGELCEGTFGIAPEWTILGEVEAEVCFVEMHLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GPZ1</accession>
    <name>A0A180GPZ1_PUCT1</name>
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        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PTTG_26863</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000035">
        <property type="protein sequence ID" value="OAV94897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblFungi" id="PTTG_26863-t43_2">
        <property type="protein sequence ID" value="PTTG_26863-t43_2-p1"/>
        <property type="gene ID" value="PTTG_26863"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_26863"/>
    <dbReference type="OrthoDB" id="2507279at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Putative RNP domain-containing protein"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF64">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="452"/>
            <end position="529"/>
        </location>
    </feature>
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            <end position="33"/>
        </location>
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        </location>
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        </location>
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    </feature>
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    </feature>
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    <feature type="compositionally biased region" description="Pro residues" evidence="3">
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    <feature type="compositionally biased region" description="Polar residues" evidence="3">
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="OAV94897.1"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblFungi" id="PTTG_26863-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GQ57</accession>
    <name>A0A180GQ57_PUCT1</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">PTTG_03019</name>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
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    <name>A0A180GQV7_PUCT1</name>
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        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
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                <person name="Birren B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">1-1 BBBD Race 1</strain>
        </source>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000037">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="PTTG_06894"/>
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            <begin position="41"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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                <person name="Zeng Q."/>
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                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
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                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
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                <person name="Howarth C."/>
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                <person name="Larson L."/>
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            <strain evidence="1">1-1 BBBD Race 1</strain>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">1-1 BBBD Race 1</strain>
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        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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            <dbReference type="PubMed" id="27913634"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
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        <source>
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<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GQX5</accession>
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    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000034">
        <property type="protein sequence ID" value="OAV94924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GQX5"/>
    <dbReference type="EnsemblFungi" id="PTTG_26810-t43_1">
        <property type="protein sequence ID" value="PTTG_26810-t43_1-p1"/>
        <property type="gene ID" value="PTTG_26810"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_26810"/>
    <dbReference type="OrthoDB" id="2496817at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF141">
        <property type="entry name" value="GH15225P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="114"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OAV94924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_26810-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="334" mass="38065" checksum="640132069A12FDB5" modified="2016-09-07" version="1">MNDEDDESDDPIDMLSSKSRTRSYVKAGSPRAPSQSLLNPTTDFPIDKNHAVTSSSQGLDLLSSHFGQLHLSQINSRTKPHQRQWPTSLTPAQQLIVDKALRPNNETIYDLPGASCGIKELQILSLPAQWINDEIINYYGSLINLQSQNQRSLKALDVHCFSSFFMSQFDLGGHQNVQRWTRKINLFQKDLILFPINLSNLHWVLGVINIQQKRFEYYDSLGGSNSKVLLKLRRYYKDEYQTKNSDNADLAQWTDYQPEVPLQSNSSDCGVFVCQFMHSLSQNSIHQDAQEPMFDFTAKNMPYLRQKMILEIISKAFLPDNSPESSNQAGTLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GRY6</accession>
    <name>A0A180GRY6_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Isochorismatase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PTTG_05214</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000033">
        <property type="protein sequence ID" value="OAV95042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GRY6"/>
    <dbReference type="EnsemblFungi" id="PTTG_05214-t43_1">
        <property type="protein sequence ID" value="PTTG_05214-t43_1-p1"/>
        <property type="gene ID" value="PTTG_05214"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_05214"/>
    <dbReference type="OrthoDB" id="269496at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050993">
        <property type="entry name" value="Isochorismatase_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14119">
        <property type="entry name" value="HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14119:SF3">
        <property type="entry name" value="ISOCHORISMATASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="3">
        <location>
            <begin position="31"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OAV95042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_05214-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="217" mass="23133" checksum="B0F16A099682E726" modified="2016-09-07" version="1">MPSHKPASPHAPPPGQPPKMNHLRLSPANAAILLCDIQERFRPKIAHFDHLEHMAEKLLKAAAALHVPVLATEQNPKALGPTVARLAQLVPPDKTFPKTQFSMLVPELAATLANPAPKHVALVGIEAHICVLQTALDLLARGIHVHVLADAVSSCNAPERALAFQTIRAAGGTVSTTEAFIYRLLADASHPRAKDIFAIVKEYSEATRDALSALGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GUB1</accession>
    <name>A0A180GUB1_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Dyp-type peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PTTG_00666</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DyP-type peroxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000020">
        <property type="protein sequence ID" value="OAV96345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GUB1"/>
    <dbReference type="EnsemblFungi" id="PTTG_00666-t43_1">
        <property type="protein sequence ID" value="PTTG_00666-t43_1-p1"/>
        <property type="gene ID" value="PTTG_00666"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00666"/>
    <dbReference type="OrthoDB" id="3207336at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049509">
        <property type="entry name" value="DyP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048328">
        <property type="entry name" value="Dyp_perox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006314">
        <property type="entry name" value="Dyp_peroxidase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01413">
        <property type="entry name" value="Dyp_perox_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30521:SF4">
        <property type="entry name" value="DEFERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30521">
        <property type="entry name" value="DEFERROCHELATASE/PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21105">
        <property type="entry name" value="DyP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20628">
        <property type="entry name" value="Dyp_perox_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51404">
        <property type="entry name" value="DYP_PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="DyP dimeric alpha+beta barrel" evidence="10">
        <location>
            <begin position="10"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Dyp-type peroxidase C-terminal" evidence="9">
        <location>
            <begin position="296"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OAV96345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00666-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="54009" checksum="B4721720966BC38D" modified="2016-09-07" version="1">MSSTNVDLKNVQGDLILGLQKRVEAFWFGTLKSEPQAIEGFRRNLKSSLLPLITTTQDVLDTQQKIQEHKRNTSPDGGQGLLPITQINLGFSFAGLKKLGLQAEEIPTGGNGIFSKGQKVDAVDNLGDPVDQATRKLKTWSRDFLSEANPIDFVVLVTAPDCKLLNERLDQLRRNFGGFLASSFVRQGNVRPGNQAGHEHFGYLDGISEPTIENINARPEDQKSGIVKPGVILLGHTTQDGNNSNLDPKQTWIKDGSFMAFRELQQLVPEFQSFCDEAAKKLQNPNVSGDFIGARIVGRWKSGAPLTLTPVEDKPELNRAQDFDFSDELKQERCPYAAHIRKTNPRNGIAGAEPNATSLPHLMVRNGIPYGPELTEEEKALKKTKENRGLLFVSYQSQIESGFQFVQKLWCNNTDFPAKTPAKVTPGFDLLIGQTSDEKPRVAQNINPLGIEGTTDPNNVLTAPAHFVVPLGGEYFLMPSIKAINEKLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GVB1</accession>
    <name>A0A180GVB1_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ferric oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_03997</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000020">
        <property type="protein sequence ID" value="OAV96444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GVB1"/>
    <dbReference type="EnsemblFungi" id="PTTG_03997-t43_1">
        <property type="protein sequence ID" value="PTTG_03997-t43_1-p1"/>
        <property type="gene ID" value="PTTG_03997"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_03997"/>
    <dbReference type="OrthoDB" id="3142841at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052979">
        <property type="entry name" value="Adenylate-forming_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV96444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_03997-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="42483" checksum="DEF3B5A26D228667" modified="2016-09-07" version="1">MLVAIAIRSEWVMRLIYWISIKSFRGWTPLKFRVAVVAILYHIGGLHSGCGISAMLWLILSWAYHIMNKNLYHYLVLVSLFLSMLVTVATCFFATPLIRGLHHNTFEITHRFLGWLGIITGLIFVIASSWWDVNTQSWRASGRYLVRQEEFWFMIAIIPLVVGQWVTVRNVPVTVQAPSNKASVIRVPGGLTSGLHTRVSRGGLREWHIFGAVSEGKHADCHYIVMAVQGGFTRAMNEEQPETLYTKTWKPCGLPYFSRLFDRGVAICTGSGIGAVGSTCIQHDNWFLVWIGADLERTYGIEFMNFIKSKIQPERLLIWDTRGPLGRPDVNVELARVYKQWGAQVALFIGSPALNKSVLRTSRARGIPVFGSIWDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GVE0</accession>
    <name>A0A180GVE0_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phytase A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Histidine acid phosphatase phyA</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Myo-inositol hexakisphosphate phosphohydrolase A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Myo-inositol-hexaphosphate 3-phosphohydrolase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PTTG_12499</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="20 21" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="20">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1D-myo-inositol 1,2,4,5,6-pentakisphosphate + H2O = 1D-myo-inositol 1,2,5,6-tetrakisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77115"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57798"/>
            <dbReference type="ChEBI" id="CHEBI:195535"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:77116"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>1D-myo-inositol 1,2,5,6-tetrakisphosphate + H2O = 1D-myo-inositol 1,2,6-trisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:195535"/>
            <dbReference type="ChEBI" id="CHEBI:195537"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:77120"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1D-myo-inositol 1,2,6-trisphosphate + H2O = 1D-myo-inositol 1,2-bisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77131"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:195537"/>
            <dbReference type="ChEBI" id="CHEBI:195539"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:77132"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1D-myo-inositol 1,2-bisphosphate + H2O = 1D-myo-inositol 2-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77135"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84142"/>
            <dbReference type="ChEBI" id="CHEBI:195539"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:77136"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1D-myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16989"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57798"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="EC" id="3.1.3.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:16990"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="ADAS02000017">
        <property type="protein sequence ID" value="OAV96730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GVE0"/>
    <dbReference type="EnsemblFungi" id="PTTG_12499-t43_2">
        <property type="protein sequence ID" value="PTTG_12499-t43_2-p1"/>
        <property type="gene ID" value="PTTG_12499"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12499"/>
    <dbReference type="OrthoDB" id="6509975at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016158">
        <property type="term" value="F:inositol hexakisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016274">
        <property type="entry name" value="Histidine_acid_Pase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF24">
        <property type="entry name" value="3-PHYTASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000894">
        <property type="entry name" value="Acid_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008110318" description="Phytase A" evidence="18">
        <location>
            <begin position="25"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="16">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="16">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="71"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="420"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OAV96730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12499-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="465" mass="52914" checksum="290A1A5FDD0AD4B1" modified="2016-09-07" version="1">MESWTSMIWTFGAVLLLLFAHSDGQGQIHLALLASEPDASRQPLPKWIVRHAGPIAPFHQIGAYEPPPQGCRIVQVNRLQRHGSRYPTAKVNKRIQRALQKIRHATHLHESLSFITNYQYHLSQDSLLPLGALESFKSGVQFASRYQKLMNPEQLPFMRASSSQRVVDSAKNFTAGLASWLGIKINNIEPLVISEDPQSNNTLDNNSCPNRESRDEKHQWLEIFGKQVTERLNLLATDAHLNNQDTLALMQLCIFDSLADRKLSRFCGIFENLDWAGYGYYYDLEKYYDHGLGNRLGQAEGIGYVAELIARLTGDRKWVKHDESKVNQTLDQSSTTFPLDQSGYVDFSHDNQMIGILAALGLQVHEPLPGTGPPPSDKIWDVSKWMPFSSRLTTEKMICGHQNKQFVRFVLNDVILIPPCKTSDKHLMLEQSPFICPLDQFIESRRGLLIRAKNLHEQCFREEPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GWE0</accession>
    <name>A0A180GWE0_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RNA exonuclease 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_26157</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Exoribonuclease involved in ribosome biosynthesis. Involved in the processing of ITS1, the internal transcribed spacer localized between the 18S and 5.8S rRNAs.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000015">
        <property type="protein sequence ID" value="OAV97147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GWE0"/>
    <dbReference type="EnsemblFungi" id="PTTG_26157-t43_2">
        <property type="protein sequence ID" value="PTTG_26157-t43_2-p1"/>
        <property type="gene ID" value="PTTG_26157"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_26157"/>
    <dbReference type="OrthoDB" id="8191639at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:rRNA processing"/>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
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                <person name="Heiman D."/>
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                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
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            <strain evidence="3">1-1 BBBD Race 1</strain>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Khalil H.B."/>
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                <person name="Sakthikumar S."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
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            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
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    <reference evidence="3" key="1">
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                <person name="Ward D."/>
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                <person name="Chapman S.B."/>
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            <strain evidence="3">1-1 BBBD Race 1</strain>
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    <reference evidence="3" key="2">
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Khalil H.B."/>
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                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
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                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="5">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
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    <reference evidence="4" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="EnsemblFungi"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
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        <property type="protein sequence ID" value="OAV96313.1"/>
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        <property type="gene ID" value="PTTG_07778"/>
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    <dbReference type="Proteomes" id="UP000005240">
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    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
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        <location>
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    </feature>
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    </evidence>
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                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
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                <person name="Heiman D."/>
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                <person name="Howarth C."/>
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                <person name="Larson L."/>
                <person name="Lewis B."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
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    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
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                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
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                <person name="Engels R."/>
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                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
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                <person name="Heiman D."/>
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                <person name="Howarth C."/>
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                <person name="Nusbaum C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
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    <reference evidence="2" key="2">
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
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            <dbReference type="EnsemblFungi" id="PTTG_26075-t43_2-p1"/>
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                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
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                <person name="Thomson T."/>
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                <person name="White J."/>
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                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
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                <person name="Nusbaum C."/>
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            <strain evidence="11">1-1 BBBD Race 1</strain>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">1-1 BBBD Race 1</strain>
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    <reference evidence="12 13" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="12">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">Isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="7"/>
    <dbReference type="EMBL" id="ADAS02000013">
        <property type="protein sequence ID" value="OAV97429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GYJ3"/>
    <dbReference type="EnsemblFungi" id="PTTG_00157-t43_2">
        <property type="protein sequence ID" value="PTTG_00157-t43_2-p1"/>
        <property type="gene ID" value="PTTG_00157"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00157"/>
    <dbReference type="OrthoDB" id="10013407at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF26">
        <property type="entry name" value="PEPTIDASE M28 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="6 7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008110442" description="Peptide hydrolase" evidence="9">
        <location>
            <begin position="38"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="10">
        <location>
            <begin position="322"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OAV97429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00157-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="513" mass="55435" checksum="66ED88A7D204916D" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GZK8</accession>
    <name>A0A180GZK8_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_02238</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000009">
        <property type="protein sequence ID" value="OAV98170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GZK8"/>
    <dbReference type="EnsemblFungi" id="PTTG_02238-t43_1">
        <property type="protein sequence ID" value="PTTG_02238-t43_1-p1"/>
        <property type="gene ID" value="PTTG_02238"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_02238"/>
    <dbReference type="OrthoDB" id="2496731at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV98170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_02238-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="426" mass="47137" checksum="3B44C3E825675050" modified="2016-09-07" version="1">MSLLYSEDSGSDGADDDSRISPEPVRQHTSTGRSSANTQESQIGPIRQSERRERNAMAGRRDAVPLRGAAAIAAAGSATTGRQSSTPGARRSTDIARPAAIPPVPGSVATGGQAPRRVGLPAIPTFPNVPFTMLQDNEPVTDAFLGELEGVFELRGGYADLAENLVHVPPPRQWAVQMYSQMSIRQAIDEARQQANLARVQAVMQPPPTVRTNDFHYVPVFSEFVKRSIRELLMSKNLKMYSSDLPRGNHRHNVRGLLSLVLDRINAQPVQFRLDYLPHGYNEAEPSAVASVERFVRSKLRNSRGKMRDLLLTDIHNTEGWEDIRPVPTISTLLAEMQTSLLPLLPNNQRNVPAGEQNAPRGHQTHMKARLAYLRIHTIGQLVSRGPRDTGRQWLNIDSHLRELDEKGREYRTSACFQLLPAGVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GZL7</accession>
    <name>A0A180GZL7_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Vacuolar protein-sorting-associated protein 36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ESCRT-II complex subunit VPS36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PTTG_00330</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the endosomal sorting complex required for transport II (ESCRT-II).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the VPS36 family.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000008">
        <property type="protein sequence ID" value="OAV98287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GZL7"/>
    <dbReference type="EnsemblFungi" id="PTTG_00330-t43_2">
        <property type="protein sequence ID" value="PTTG_00330-t43_2-p1"/>
        <property type="gene ID" value="PTTG_00330"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_00330"/>
    <dbReference type="OrthoDB" id="271448at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000416">
        <property type="entry name" value="Vacuolar protein-sorting-associated protein 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021648">
        <property type="entry name" value="GLUE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040608">
        <property type="entry name" value="Snf8/Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037855">
        <property type="entry name" value="Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13128">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13128:SF12">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04157">
        <property type="entry name" value="EAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51495">
        <property type="entry name" value="GLUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GLUE N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="51"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="148"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="447"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="505"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OAV98287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_00330-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="559" mass="61485" checksum="BC73C5EDD440F554" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180GZN5</accession>
    <name>A0A180GZN5_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mannosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PTTG_25964</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the third mannose to Man2-GlcN-acyl-PI during GPI precursor assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 22 family. PIGB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="ADAS02000011">
        <property type="protein sequence ID" value="OAV97788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180GZN5"/>
    <dbReference type="EnsemblFungi" id="PTTG_25964-t43_1">
        <property type="protein sequence ID" value="PTTG_25964-t43_1-p1"/>
        <property type="gene ID" value="PTTG_25964"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_25964"/>
    <dbReference type="OrthoDB" id="416834at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF4">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="95"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="189"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="343"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="368"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OAV97788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_25964-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="479" mass="53999" checksum="1A9D9E4E2648D4B5" modified="2016-09-07" version="1">MRPLRQLACSLTSLFNAYAGVRTFSNSLETALTASALAYWPWNAHEIPHSWANLSLSILLIALSVIVRPSAILFWSVLGYTLLKKSTGEARMDVMALVAVLGVTSALACLLIDSSFYGLVTFTPLNFIRQNVLNDLSTFYGVNRWHFYFTQAFTFVNFSMFPTVILGMLSLITPQNVHESPGQLDRLRSARVAVLGTMLGLSLLRHKEFRFIAPLVPLFNCFAARAMVNNYIKDRLARPASSPADISRLERILSARPLGFILRSSVAFAAAVYLLGFHYRAQIAVIDYLRQLPDLELKSVGFLMPCHSTPWQSHLHKPHLAPNGTQERLWMLTCEPPLISFRNSSSTEADGAANSTAHANQTEDTTEERERKEQKAESKQLDDPGEYEDEAAEFYRDPPGFLTARFPETVDKGYPATDKSTGRYQWPSHLVLFDALWTLHPVRSLLQDRGYRVVWNMTNGWWHDDPQRRAGNVIVLRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H0T5</accession>
    <name>A0A180H0T5_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_01756</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
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            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
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                <person name="Pearson M."/>
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                <person name="Nusbaum C."/>
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            <strain evidence="5">1-1 BBBD Race 1</strain>
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        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">1-1 BBBD Race 1</strain>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
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            <dbReference type="PubMed" id="27913634"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="7">Isolate 1-1 / race 1 (BBBD)</strain>
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    <reference evidence="6" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
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        <property type="gene ID" value="PTTG_07696"/>
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    <dbReference type="OrthoDB" id="6075923at2759"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Gate_dom"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
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                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
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                <person name="Birren B."/>
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            <strain evidence="18">1-1 BBBD Race 1</strain>
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    </reference>
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        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="18">1-1 BBBD Race 1</strain>
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        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
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            <strain evidence="19">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="20">Isolate 1-1 / race 1 (BBBD)</strain>
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    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
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            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
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        <subcellularLocation>
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            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
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        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CLASP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the TOG/XMAP215 family.</text>
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        <source>
            <dbReference type="EMBL" id="OAV98650.1"/>
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    <evidence type="ECO:0000313" key="20">
        <source>
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        <name evidence="2" type="ORF">PTTG_25620</name>
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    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
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        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">1-1 BBBD Race 1</strain>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
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    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
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            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000006">
        <property type="protein sequence ID" value="OAV98707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H0X8"/>
    <dbReference type="EnsemblFungi" id="PTTG_25620-t43_1">
        <property type="protein sequence ID" value="PTTG_25620-t43_1-p1"/>
        <property type="gene ID" value="PTTG_25620"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_25620"/>
    <dbReference type="OrthoDB" id="2495233at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019193">
        <property type="entry name" value="UBQ-conj_enz_E2-bd_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31531:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE E3D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31531">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE E3D FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09814">
        <property type="entry name" value="HECT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV98707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_25620-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="420" mass="46711" checksum="CAD28EABE624E272" modified="2016-09-07" version="1">METGDGVAVEIGDERVEIYVGAQLDISQHTVEVKTRTKIEIGLKTFPPGDAKREDRFEKTSARFGLARLKNDGVGRLCCGRCRNELVDGLDGADVRWYALPSEDWAEFVEYWVCHEDMKVKAGDSVGCGAGNGLRKPRPREGFLGDWFLLLDLDWVENIASQGSKSIGWRRQAPQKAAHQPTRKHFVECNRCGFTLGEVEEDVTRHEPPASADKILENPEGGPSGPNRRWIRFYKRAISSSTHWHAQDDEADGRGFEISLIEDEVSKLVESNGFYKVAVKNMGDSRSIGLWIFCPSVLIRCRRRPAGGDARQPLSRAKLMYVVFEGDGSPADLATMERFETGARNSLGSERRIGEICVPADHFELLLAKLSLGSIYRIGQPIAPFSPPGPVPVSQPLDDRVSGADDDEDLFQGIYRVAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H165</accession>
    <name>A0A180H165_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PTTG_07131</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="14">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:47005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="ADAS02000007">
        <property type="protein sequence ID" value="OAV98501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H165"/>
    <dbReference type="EnsemblFungi" id="PTTG_07131-t43_1">
        <property type="protein sequence ID" value="PTTG_07131-t43_1-p1"/>
        <property type="gene ID" value="PTTG_07131"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_07131"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000016">
        <property type="entry name" value="Protein phosphatase 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF565">
        <property type="entry name" value="AT28366P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="12">
        <location>
            <begin position="23"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="324"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="373"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OAV98501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_07131-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="400" mass="43589" checksum="AB6C4F4995880903" modified="2016-09-07" version="1">MGQTLSEPIIEKHTSAGEDEDFAFGVSEMQGWRLTMEDAHAAVLDLNRAPPNSSSTPSVSTDPSKKRTRFFAVYDGHGGATVAKFSGDTVHFRLRSTPQYQSGDYEAALKRAFLATDEDLRSNPDFANDPSGCTAVAALITPDGKILVANAGDSRSVLCVNGVAEPMSHDHKPVNRDENNRIVAAGGFVEFGRVNGNLALSRAIGDFEFKQNKDLSPEAQVVTANPDILTHQITVEDEFLILACDGIWDVYSNQQVVDRVRRLLGERKTLEQVAEQMIDYCLAPDCEWGGVGCDNMTFMVVAILGGKTKEQWYDMICQRLERGEGHQTPSNFPQPYARGPRGEMIPNIMRLGDSPDDRTSPPGYNQDSEDHSTNAASGNTGSSLFSLAPVSIIEALSRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H169</accession>
    <name>A0A180H169_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_00297</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
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                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
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                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
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                <person name="Joly D."/>
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                <person name="Sakthikumar S."/>
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                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
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                <person name="Zeng Q."/>
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                <person name="Bochicchio J."/>
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                <person name="Chapman S.B."/>
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                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
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                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
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                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
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                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="3">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000006">
        <property type="protein sequence ID" value="OAV98612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H276"/>
    <dbReference type="EnsemblFungi" id="PTTG_09276-t43_2">
        <property type="protein sequence ID" value="PTTG_09276-t43_2-p1"/>
        <property type="gene ID" value="PTTG_09276"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_09276"/>
    <dbReference type="OrthoDB" id="2527403at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018803">
        <property type="entry name" value="Ish1/Msc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33939:SF1">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33939">
        <property type="entry name" value="PROTEIN CBG22215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10281">
        <property type="entry name" value="Ish1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OAV98612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_09276-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="616" mass="68405" checksum="026BE87322EFD0C9" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H2V5</accession>
    <name>A0A180H2V5_PUCT1</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AMP deaminase</fullName>
            <ecNumber evidence="4">3.5.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Myoadenylate deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PTTG_07701</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="14">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.6" evidence="4"/>
    <dbReference type="EMBL" id="ADAS02000006">
        <property type="protein sequence ID" value="OAV98683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H2V5"/>
    <dbReference type="EnsemblFungi" id="PTTG_07701-t43_2">
        <property type="protein sequence ID" value="PTTG_07701-t43_2-p1"/>
        <property type="gene ID" value="PTTG_07701"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_07701"/>
    <dbReference type="OrthoDB" id="1723809at2759"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00663"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003876">
        <property type="term" value="F:AMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01319">
        <property type="entry name" value="AMPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.20:FF:000001">
        <property type="entry name" value="AMP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006650">
        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006329">
        <property type="entry name" value="AMPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01429">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359:SF0">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19326">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001251">
        <property type="entry name" value="AMP_deaminase_met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="95"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OAV98683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_07701-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="777" mass="90119" checksum="295D9C2840B2CD29" modified="2016-09-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H2X7</accession>
    <name>A0A180H2X7_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PTTG_12187</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="4">Isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="ADAS02000003">
        <property type="protein sequence ID" value="OAV99367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H2X7"/>
    <dbReference type="EnsemblFungi" id="PTTG_12187-t43_1">
        <property type="protein sequence ID" value="PTTG_12187-t43_1-p1"/>
        <property type="gene ID" value="PTTG_12187"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_12187"/>
    <dbReference type="OrthoDB" id="3267335at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OAV99367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_12187-t43_1-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="448" mass="48612" checksum="B13F25613E2FD2C0" modified="2016-09-07" version="1">MQGERGSSVKHMNFVIPECTPPGDYQVIFHELDRINGKQFYSIYSLPFHVLSPQEKAGTESVDAAEQSKCDKFTVPQAELRDSRPSKQPFVKKGVTGFKLQKGKIPLPKDEDIFVPEDEVTTLVVQGTATAKPASPAEDATPAAPNSKEKFTPPVEKSRPSVEKPTPPAETETPVPQPKPPAQAENPAPSLAQPTNRDQKAAETAVTDTAKSQVKKTPLLEQNSSQEAQERSKGKTTDPEDDHDEESLDSTLRISSGTSADSPELSRQAEVDKNVKIEAKKTKLSVGSPSPTAQLETPPPDSNSGNKKPAITFTESDYSLDKKPTKIQGDEEIEESEVENSKADLLRKASVIPDVEAPLVSSRLDPSISEAEKTGQNITVVEAVVNHGILGSASQGNMAILGQSTNQMVFSVPLIAPSTSDCNIMSLHRWKNTLFLPWALYSIIKLWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A180H5B4</accession>
    <name>A0A180H5B4_PUCT1</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Calpain catalytic domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PTTG_11706</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Puccinia triticina (isolate 1-1 / race 1 (BBBD))</name>
        <name type="common">Brown leaf rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="630390"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2009-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Ward D."/>
                <person name="Feldgarden M."/>
                <person name="Earl A."/>
                <person name="Young S.K."/>
                <person name="Zeng Q."/>
                <person name="Koehrsen M."/>
                <person name="Alvarado L."/>
                <person name="Berlin A."/>
                <person name="Bochicchio J."/>
                <person name="Borenstein D."/>
                <person name="Chapman S.B."/>
                <person name="Chen Z."/>
                <person name="Engels R."/>
                <person name="Freedman E."/>
                <person name="Gellesch M."/>
                <person name="Goldberg J."/>
                <person name="Griggs A."/>
                <person name="Gujja S."/>
                <person name="Heilman E."/>
                <person name="Heiman D."/>
                <person name="Hepburn T."/>
                <person name="Howarth C."/>
                <person name="Jen D."/>
                <person name="Larson L."/>
                <person name="Lewis B."/>
                <person name="Mehta T."/>
                <person name="Park D."/>
                <person name="Pearson M."/>
                <person name="Roberts A."/>
                <person name="Saif S."/>
                <person name="Shea T."/>
                <person name="Shenoy N."/>
                <person name="Sisk P."/>
                <person name="Stolte C."/>
                <person name="Sykes S."/>
                <person name="Thomson T."/>
                <person name="Walk T."/>
                <person name="White J."/>
                <person name="Yandava C."/>
                <person name="Izard J."/>
                <person name="Baranova O.V."/>
                <person name="Blanton J.M."/>
                <person name="Tanner A.C."/>
                <person name="Dewhirst F.E."/>
                <person name="Haas B."/>
                <person name="Nusbaum C."/>
                <person name="Birren B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Szabo L."/>
                <person name="Khalil H."/>
                <person name="Joly D."/>
                <person name="Goldberg J."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Fellers J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">1-1 BBBD Race 1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Isolate 1-1 / race 1 (BBBD)</strain>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblFungi"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">isolate 1-1 / race 1 (BBBD)</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family. PalB/RIM13 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="ADAS02000002">
        <property type="protein sequence ID" value="OAV99769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A180H5B4"/>
    <dbReference type="EnsemblFungi" id="PTTG_11706-t43_2">
        <property type="protein sequence ID" value="PTTG_11706-t43_2-p1"/>
        <property type="gene ID" value="PTTG_11706"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="FungiDB:PTTG_11706"/>
    <dbReference type="OrthoDB" id="2501585at2759"/>
    <dbReference type="Proteomes" id="UP000005240">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022683">
        <property type="entry name" value="Calpain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051297">
        <property type="entry name" value="PalB/RIM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46143">
        <property type="entry name" value="CALPAIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46143:SF1">
        <property type="entry name" value="CALPAIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="7">
        <location>
            <begin position="4"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OAV99769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblFungi" id="PTTG_11706-t43_2-p1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000005240"/>
        </source>
    </evidence>
    <sequence length="738" mass="82294" checksum="4B8DA0B255A7266D" modified="2016-09-07" version="1"> </sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>